The Experts below are selected from a list of 132 Experts worldwide ranked by ideXlab platform
J.p. Bhatt - One of the best experts on this subject based on the ideXlab platform.
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Photoelectrochemical studies on Halobacterium halobium for continuous production of hydrogen
International Journal of Hydrogen Energy, 2003Co-Authors: M.m.taqui Khan, J.p. BhattAbstract:Continuous hydrogen evolution was obtained photoelectrochemically using Halobacterium halobium as catalyst at −2 V vs SCE. Deacedite FF resin was used to control the pH of the system.
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Large scale photobiological solar hydrogen generation using Halobacterium halobium MMT22 and silicon cell
International Journal of Hydrogen Energy, 1992Co-Authors: M.m.taqui Khan, M.r. Adiga, J.p. BhattAbstract:Abstract This biophotoelectrochemical system produces about twice the amount of H 2 compared with that of a solar photovoltaic hydrogen system due to the low requirement of reduction potential of ≥ − 0.41 V. Halobacterium halobium MMT 22 covered on 1 m 2 area produces about 11.36 1 h −1 under solar insolation of 70–80 mW cm −2 .
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Photosensitized continuous production of hydrogen by Halobacterium halobium MMT22 coupled to Escherichia coli
International Journal of Hydrogen Energy, 1991Co-Authors: M.m.taqui Khan, J.p. BhattAbstract:Abstract Continuous evolution of hydrogen was obtained for 12 weeks in a specially designed “chemostat” by Halobacterium halobium MMT22 coupled to Escherichia coli.
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Enhanced production of formic acid by Halobacterium halobium MMT22 by reduction of hydrogencarbonate in aqueous solution
Bulletin of the Chemical Society of Japan, 1991Co-Authors: Mirza Mohammad Taqui Khan, J.p. BhattAbstract:Enhanced production of formic acid was observed by reduction of hydrogencarbonate in aqueous solution by Halobacterium halobium MMT22. A one and half fold increase in the production of formic acid was observed in the presence of ascorbic acid as electron donor and [Ru(Bipy)3]2+ as photosensitizer.
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Polyethylene glycol mediated fusion of Halobacterium halobium MMT22 and Escherichia coli for enhancement of hydrogen production
International Journal of Hydrogen Energy, 1991Co-Authors: M.m.taqui Khan, J.p. BhattAbstract:Abstract A simplified system with enhanced rates of hydrogen production was obtained by polyethylene glycol mediated fusion of Halobacterium halobium MMT22 and Escherichia coli. A continuous evolution of hydrogen was observed at the rate of 60.2 μmol mg−1 cells min−1 for about 2 weeks by fusion product 1.
Ignat Ignatov - One of the best experts on this subject based on the ideXlab platform.
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improved method for isolation of photochrome transmembrane protein bacteriorhodopsin from purple membranes of Halobacterium Halobacterium halobium
Journal of Medicine Physiology and Biophysics, 2015Co-Authors: Oleg Mosin, Ignat IgnatovAbstract:This paper presents improved method for isolation of photochrome transmembraine protein bacteriorhodopsin (output 5 mg from 100 g of wet biomass) capable to transform light energy to electrochemical energy of generated protons H + and ??P. The protein was isolated from purple membranes of photo-organotrophic Halobacterium Halobacterium halobium by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of low and high-weight molecular impurities, cellular RNA, carotenoids and lipids, solubilization with 0.5% (w/v) SDS-Na, fractionation by MeOH and column g el permeation chromatography (GPC) of the final protein on Sephadex G-200 with 0.1% (w/v) SDS-Na and 2.5 mM ETDA. The homogeneity of the isolated BR was proved by combination of preparative and analytical methods including elecrtophoresis in 12.5% (w/v) PAAG with 0.1% (w/v) SDS-Na and regeneration of apomembranes with 13- trans -retinal. Keywords: Halobacterium halobium , purple membranes, bacteriorhodopsin, biosynthesis, biomolecular electronics
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The Natural Phototransforming Photochrome Membrane Protein Bacteriorhodopsin From Purple Membranes of Halobacterium Halobacterium halobium
Nanotechnology Research and Practice, 2014Co-Authors: Oleg Mosin, Ignat IgnatovAbstract:The article presents the technology of microbiological synthesis of natural phototransforming photochrome transmembraine protein bacteriorhodopsin (output 8-10 mg) from purple membranes of photo-organotrophic Halobacterium Halobacterium halobium, which is capable to transform light energy to electrochemical energy of generated protons H + and adenosine-5 ' -triphosphate (АТP). These data are important for nanoindustry of new modern domestic phototransforming nanomaterials on the base of bacteriorhodopsin and molecular bioelectronics.
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photochrome transmembrane protein bacteriorhodopsin from purple membranes of Halobacterium halobium in nano and biotechnologies
Journal of Medicine Physiology and Biophysics, 2014Co-Authors: Oleg Mosin, Ignat IgnatovAbstract:This paper presents improved method for isolation of photochrome transmembraine protein bacteriorhodopsin (output 5 mg from 100 g of wet biomass) capable to transform light energy to electrochemical energy of generated protons H + and ??P. The protein was isolated from purple membranes of photo-organotrophic Halobacterium Halobacterium halobium by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of low and high-weight molecular impurities, cellular RNA, carotenoids and lipids, solubilization with 0.5% (w/v) SDS-Na, fractionation by MeOH and column g el permeation chromatography (GPC) of the final protein on Sephadex G-200 with 0.1% (w/v) SDS-Na and 2.5 mM ETDA. The homogeneity of the isolated BR was proved by combination of preparative and analytical methods including elecrtophoresis in 12.5% (w/v) PAAG with 0.1% (w/v) SDS-Na and regeneration of apomembranes with 13- trans -retinal. Keywords: Halobacterium halobium , purple membranes, bacteriorhodopsin, biosynthesis, biomolecular electronics
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isolation of photochrome transmembrane protein bacteriorhodopsin from purple membranes of Halobacterium Halobacterium halobium new method for isolation
Journal of Health Medicine and Nursing, 2014Co-Authors: Oleg Mosin, Ignat IgnatovAbstract:This paper presents improved method for isolation of photochrome transmembraine protein bacteriorhodopsin (output 5 mg from 100 g of wet biomass) capable to transform light energy to electrochemical energy of generated protons H + and ??P. The protein was isolated from purple membranes of photo-organotrophic Halobacterium Halobacterium halobium by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of low and high-weight molecular impurities, cellular RNA, carotenoids and lipids, solubilization with 0.5% (w/v) SDS-Na, fractionation by MeOH and column g el permeation chromatography (GPC) of the final protein on Sephadex G-200 with 0.1% (w/v) SDS-Na and 2.5 mM ETDA. The homogeneity of the isolated BR was proved by combination of preparative and analytical methods including elecrtophoresis in 12.5% (w/v) PAAG with 0.1% (w/v) SDS-Na and regeneration of apomembranes with 13- trans -retinal. Keywords: Halobacterium halobium , purple membranes, bacteriorhodopsin, biosynthesis, biomolecular electronics
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Halobacterium Halobacterium halobium. Biosynthesis of Deuterium-labeled Transmembrane Protein bacteriorhodopsin from Halobacterium halobium
Journal of Health Medicine and Nursing, 2014Co-Authors: Oleg Mosin, Ignat IgnatovAbstract:The semi-preparative biosynthesis of photochrome transmembrane protein bacteriorhodopsin (output 8 - 10 mg) , labeled with deuterium on functionally important amino acid residues - [2,3,4,5,6- 2 H 5 ]phenylalanine, [3,5- 2 H 2 ]tyrosine, and [2,4,5,6,7- 2 H 5 ]tryptophan was carried out with using a photo-organotrophic Halobacterium Halobacterium halobium . The protein was isolated from purple membranes by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of low and high-weight molecular impurities, cellular RNA, carotenoids and lipids, with the subsequent s olubilization of final product with 0.5% (w/v) SDS-Na and fractionation by methanol. T he homogeneity of the synthesized product, and the selectivity of deuterium incorporation into the molecule was proved by combination of preparative and analytical protein methods including elecrtophoresis in 12.5% (w/v) PAAG with 0.1% (w/v) SDS-Na, gel filtration chromatography on Sephadex G-200, and electron impact mass-spectrometry of methyl esters of N-Dns-[ 2 H ]derivatives of amino acids after their separation by reverse-phase HPLC. Keywords : Halobacterium halobium, b acteriorhodopsin, [2,3,4,5,6- 2 H 5 ]Phe, [3,5- 2 H 2 ]Tyr, [2,4,5,6,7- 2 H 5 ]Trp, biosynthesis, EI mass-spectrometry, RP-HPLC.
M L Salin - One of the best experts on this subject based on the ideXlab platform.
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Elevation of superoxide dismutase in Halobacterium halobium by heat shock.
Journal of bacteriology, 1991Co-Authors: G B Begonia, M L SalinAbstract:Abstract Exposure of Halobacterium halobium to 50 degrees C for 2.5 h in an aerobic environment resulted in a greater than twofold increase in the activity of the manganese-containing superoxide dismutase. Nondenaturing polyacrylamide gels stained for enzymatic activity did not reveal any additional isozymes of superoxide dismutase induced by the heat shock. The maximal effect was observed at 50 degrees C, and the elevated levels of activity remained constant during 5 h of recovery at 40 degrees C. The induction of enzymatic activity was sensitive to protein synthesis inhibitors. The results are discussed relative to heat shock and stress-related proteins as well as alterations in metabolism brought about by elevated temperatures.
Janos K Lanyi - One of the best experts on this subject based on the ideXlab platform.
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Halorhodopsin — a second retinal pigment in Halobacterium halobium
Trends in Biochemical Sciences, 2003Co-Authors: Janos K LanyiAbstract:Abstract A light-driven sodium pump has been identified in Halobacterium halobium membranes. It appears to be a retinal protein, with properties similar to those of bacteriorhodopsin. The pigment may play an important role in the presence of this microorganism to illumination.
Oleg Mosin - One of the best experts on this subject based on the ideXlab platform.
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improved method for isolation of photochrome transmembrane protein bacteriorhodopsin from purple membranes of Halobacterium Halobacterium halobium
Journal of Medicine Physiology and Biophysics, 2015Co-Authors: Oleg Mosin, Ignat IgnatovAbstract:This paper presents improved method for isolation of photochrome transmembraine protein bacteriorhodopsin (output 5 mg from 100 g of wet biomass) capable to transform light energy to electrochemical energy of generated protons H + and ??P. The protein was isolated from purple membranes of photo-organotrophic Halobacterium Halobacterium halobium by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of low and high-weight molecular impurities, cellular RNA, carotenoids and lipids, solubilization with 0.5% (w/v) SDS-Na, fractionation by MeOH and column g el permeation chromatography (GPC) of the final protein on Sephadex G-200 with 0.1% (w/v) SDS-Na and 2.5 mM ETDA. The homogeneity of the isolated BR was proved by combination of preparative and analytical methods including elecrtophoresis in 12.5% (w/v) PAAG with 0.1% (w/v) SDS-Na and regeneration of apomembranes with 13- trans -retinal. Keywords: Halobacterium halobium , purple membranes, bacteriorhodopsin, biosynthesis, biomolecular electronics
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The Natural Phototransforming Photochrome Membrane Protein Bacteriorhodopsin From Purple Membranes of Halobacterium Halobacterium halobium
Nanotechnology Research and Practice, 2014Co-Authors: Oleg Mosin, Ignat IgnatovAbstract:The article presents the technology of microbiological synthesis of natural phototransforming photochrome transmembraine protein bacteriorhodopsin (output 8-10 mg) from purple membranes of photo-organotrophic Halobacterium Halobacterium halobium, which is capable to transform light energy to electrochemical energy of generated protons H + and adenosine-5 ' -triphosphate (АТP). These data are important for nanoindustry of new modern domestic phototransforming nanomaterials on the base of bacteriorhodopsin and molecular bioelectronics.
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photochrome transmembrane protein bacteriorhodopsin from purple membranes of Halobacterium halobium in nano and biotechnologies
Journal of Medicine Physiology and Biophysics, 2014Co-Authors: Oleg Mosin, Ignat IgnatovAbstract:This paper presents improved method for isolation of photochrome transmembraine protein bacteriorhodopsin (output 5 mg from 100 g of wet biomass) capable to transform light energy to electrochemical energy of generated protons H + and ??P. The protein was isolated from purple membranes of photo-organotrophic Halobacterium Halobacterium halobium by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of low and high-weight molecular impurities, cellular RNA, carotenoids and lipids, solubilization with 0.5% (w/v) SDS-Na, fractionation by MeOH and column g el permeation chromatography (GPC) of the final protein on Sephadex G-200 with 0.1% (w/v) SDS-Na and 2.5 mM ETDA. The homogeneity of the isolated BR was proved by combination of preparative and analytical methods including elecrtophoresis in 12.5% (w/v) PAAG with 0.1% (w/v) SDS-Na and regeneration of apomembranes with 13- trans -retinal. Keywords: Halobacterium halobium , purple membranes, bacteriorhodopsin, biosynthesis, biomolecular electronics
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isolation of photochrome transmembrane protein bacteriorhodopsin from purple membranes of Halobacterium Halobacterium halobium new method for isolation
Journal of Health Medicine and Nursing, 2014Co-Authors: Oleg Mosin, Ignat IgnatovAbstract:This paper presents improved method for isolation of photochrome transmembraine protein bacteriorhodopsin (output 5 mg from 100 g of wet biomass) capable to transform light energy to electrochemical energy of generated protons H + and ??P. The protein was isolated from purple membranes of photo-organotrophic Halobacterium Halobacterium halobium by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of low and high-weight molecular impurities, cellular RNA, carotenoids and lipids, solubilization with 0.5% (w/v) SDS-Na, fractionation by MeOH and column g el permeation chromatography (GPC) of the final protein on Sephadex G-200 with 0.1% (w/v) SDS-Na and 2.5 mM ETDA. The homogeneity of the isolated BR was proved by combination of preparative and analytical methods including elecrtophoresis in 12.5% (w/v) PAAG with 0.1% (w/v) SDS-Na and regeneration of apomembranes with 13- trans -retinal. Keywords: Halobacterium halobium , purple membranes, bacteriorhodopsin, biosynthesis, biomolecular electronics
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Halobacterium Halobacterium halobium. Biosynthesis of Deuterium-labeled Transmembrane Protein bacteriorhodopsin from Halobacterium halobium
Journal of Health Medicine and Nursing, 2014Co-Authors: Oleg Mosin, Ignat IgnatovAbstract:The semi-preparative biosynthesis of photochrome transmembrane protein bacteriorhodopsin (output 8 - 10 mg) , labeled with deuterium on functionally important amino acid residues - [2,3,4,5,6- 2 H 5 ]phenylalanine, [3,5- 2 H 2 ]tyrosine, and [2,4,5,6,7- 2 H 5 ]tryptophan was carried out with using a photo-organotrophic Halobacterium Halobacterium halobium . The protein was isolated from purple membranes by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of low and high-weight molecular impurities, cellular RNA, carotenoids and lipids, with the subsequent s olubilization of final product with 0.5% (w/v) SDS-Na and fractionation by methanol. T he homogeneity of the synthesized product, and the selectivity of deuterium incorporation into the molecule was proved by combination of preparative and analytical protein methods including elecrtophoresis in 12.5% (w/v) PAAG with 0.1% (w/v) SDS-Na, gel filtration chromatography on Sephadex G-200, and electron impact mass-spectrometry of methyl esters of N-Dns-[ 2 H ]derivatives of amino acids after their separation by reverse-phase HPLC. Keywords : Halobacterium halobium, b acteriorhodopsin, [2,3,4,5,6- 2 H 5 ]Phe, [3,5- 2 H 2 ]Tyr, [2,4,5,6,7- 2 H 5 ]Trp, biosynthesis, EI mass-spectrometry, RP-HPLC.
