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Shuang-jiang Liu - One of the best experts on this subject based on the ideXlab platform.
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Halorubrum litoreum sp. nov., an extremely Halophilic Archaeon from a solar saltern.
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Heng-lin Cui, Pei-jin Zhou, Ze-ying Lin, Ying Dong, Shuang-jiang LiuAbstract:An extremely Halophilic Archaeon, strain Fa-1(T), was isolated from a marine solar saltern in Fujian, China. Strain Fa-1(T) required Mg2+ and at least 2.0 M NaCl for growth. It was able to grow at pH 6.5-9.0 (optimally at pH 7.0-7.5) and at 20-55 degrees C (optimally at 37-42 degrees C). The major polar lipids of strain Fa-1(T) were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and a sulfated diglycosyl diether. On the basis of a 16S rRNA gene sequence analysis, strain Fa-1(T) was closely related to nine species of the genus Halorubrum, showing sequence similarities of 97.4-98.4 %. The G+C content of the DNA of strain Fa-1(T) is 64.9 mol% (Tm). DNA-DNA hybridization values between strain Fa-1(T) and the most closely related members of the genus Halorubrum were below 51 %. On the basis of the data from this study, strain Fa-1(T) represents a novel species of the genus Halorubrum, for which the name Halorubrum litoreum sp. nov. is proposed. The type strain is Fa-1(T) (=CGMCC 1.5336(T) =JCM 13561(T)).
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haloarcula amylolytica sp nov an extremely Halophilic Archaeon isolated from aibi salt lake in xin jiang china
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Yong Yang, Pei-jin Zhou, Heng-lin Cui, Shuang-jiang LiuAbstract:A starch-hydrolysing and extremely Halophilic Archaeon (strain BD-3(T)), isolated from Aibi salt lake in Xin-Jiang, China, was characterized phenotypically and genotypically in order to determine its taxonomic status. On the basis of its polar lipid composition, nucleotide sequences of its 16S rRNA genes, genomic DNA G+C content (62.4 mol%) and growth characteristics, the organism could be assigned to the genus Haloarcula. Phenotypic differences and low DNA-DNA hybridization values to related Haloarcula species distinguished strain BD-3(T) from recognized Haloarcula species. It is therefore concluded that strain BD-3(T) represents a novel species, for which the name Haloarcula amylolytica sp. nov. is proposed. The type strain is BD-3(T) (=CGMCC 1.5335(T)=JCM 13557(T)).
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halobacterium jilantaiense sp nov a Halophilic Archaeon isolated from a saline lake in inner mongolia china
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Yong Yang, Pei-jin Zhou, Heng-lin Cui, Shuang-jiang LiuAbstract:A novel Halophilic Archaeon, NG4(T), was isolated from Jilantai salt lake in Inner Mongolia, China. The taxonomy of strain NG4(T) was studied by polyphasic methods. Strain NG4(T) grew at pH 5.5-8.5 and at a temperature of 22-55 degrees C. It was chemo-organotrophic, aerobic and required concentrations of 2.7-5.2 M NaCl and 0.05-0.3 M Mg(2+) for growth. Cells were Gram-negative, slender rods. Colonies on agar plates containing 25 % (w/v) total salts were red, elevated and round. According to 16S rRNA gene sequence similarity, strain NG4(T) was phylogenetically related to Halobacterium salinarum DSM 3754(T) (98.2 %) and Halobacterium noricense A1(T) (97.3 %). The DNA G+C content was 64.2 mol%. DNA-DNA relatedness values with Hbt. salinarum DSM 3754(T) and Hbt. noricense A1(T) were 47 and 35 %, respectively. The polar lipids of strain NG4(T) consisted of phosphatidylglycerol, methylated phosphatidylglycerol phosphate, phosphatidylglycerol sulfate, triglycosyl diether, sulfated triglycosyl diether and sulfated tetraglycosyl diether. It was concluded that strain NG4(T) represents a novel species of the genus Halobacterium, for which the name Halobacterium jilantaiense sp. nov. is proposed. The type strain is NG4(T) (=CGMCC 1.5337(T)=JCM 13558(T)).
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natronorubrum aibiense sp nov an extremely Halophilic Archaeon isolated from aibi salt lake in xin jiang china and emended description of the genus natronorubrum
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Heng-lin Cui, Pei-jin Zhou, Dilbr Tohty, Jie Feng, Shuang-jiang LiuAbstract:An extremely Halophilic Archaeon (strain 7-3(T)) was isolated from Aibi salt lake in Xin-Jiang, China. It formed small (<1.0 mm), red, elevated colonies on salt-milk agar medium. Strain 7-3(T) was able to grow at pH 6.5-9.5 (optimum pH 8.0) and at 20-50 degrees C (optimum 45 degrees C). Analysis of its 16S rRNA gene sequence indicated that strain 7-3(T) was phylogenetically related to members of the genus Natronorubrum, with sequence similarities of 97.0 % to Natronorubrum bangense A33(T) and 95.2 % to Natronorubrum tibetense GA33(T). The G+C content of its DNA was 61.2 mol% (T(m)). Levels of DNA-DNA relatedness to Nrr. bangense and Nrr. tibetense were 45 and 37 %, respectively. It is concluded that strain 7-3(T) (=CGMCC 1.4299(T)=JCM 13488(T)) should be classified as the type strain of a novel species of the genus Natronorubrum, for which the name Natronorubrum aibiense sp. nov. is proposed. Based on the properties of Nrr. aibiense given here and of those of Nrr. bangense and Nrr. tibetense described previously, an emended description of the genus Natronorubrum is presented.
Heng-lin Cui - One of the best experts on this subject based on the ideXlab platform.
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complete genome sequence of salinigranum rubrum gx10t an extremely Halophilic Archaeon isolated from a marine solar saltern
Marine Genomics, 2019Co-Authors: Shuaibo Han, Heng-lin Cui, Xinjun Hou, Zhe Zhao, Ran Zhang, Lawrence Jordan KeenAbstract:Abstract Since the first genome of a Halophilic Archaeon was sequenced in 2000, microbes inhabiting hypersaline environments have been investigated largely based on genomic characteristics. Salinigranum rubrum GX10T, the type species of the genus Salinigranum belonging to the euryarchaeal family Haloferacaceae, was isolated from the brine of Gangxi marine solar saltern near Weihai, China. Similar with most members of the class Halobacteria, S. rubrum GX10T is an extreme halophile requiring at least 1.5 M NaCl for growth and 3.1 M NaCl for optimum growth. We sequenced and annotated the complete genome of S. rubrum GX10T, which was found to be 4,973,118 bp and comprise one chromosome and five plasmids. A total of 4966 protein coding genes, 47 tRNA genes and 6 rRNA genes were obtained. The isoelectric point distribution for the predict proteins was observed with an acidic peak, which reflected the adaption of S. rubrum GX10T to the Halophilic environment. Genes related to potassium uptake, sodium efflux as well as compatible-solute biosynthesis and transport were identified, which were responsible for the resistance to osmotic stress. Genes related to heavy metal resistance, CRISPR-Cas system and light transform system were also detected. This study reports the first genome in the genus Salinigranum and provides a basis for understanding resistance strategies to harsh environment at the genomic level.
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purification and characterisation of a salt stable protease from the Halophilic Archaeon halogranum rubrum
Journal of the Science of Food and Agriculture, 2017Co-Authors: Ruichang Gao, Heng-lin Cui, Tong Shi, Xiangdong Liu, Mengqin Zhao, Li YuanAbstract:Background: Because proteases play an important role in the fermentation of fish sauce, the purification and characterisation of an extracellular protease from the Halophilic Archaeon Halogranum rubrum was investigated.; Results: The molecular mass of the protease was estimated to be approximately 47 kDa based on sodium dodecyl sulfate-polyacrylamide gel electropheresis (SDS-PAGE) and native-PAGE analysis. The optimum conditions for catalytic activity were pH 8.0 and 50°C. The protease showed alkaline stability (pH 7.0-10.0). The protease also exhibited novel catalytic ability over a broad range of salinity (NaCl 0-3 mol L-1 ). Calcium ion enhanced the proteolytic activity of the enzyme. The Km and Vmax values of the purified protease for casein were calculated to be 4.89 mg mL-1 and 1111.11 U mL-1 , respectively. The protease was strongly inhibited by ethylenediamine tetraacetic acid (EDTA) and phenylmethanesulfonyl fluoride (PMSF). Meanwhile, the protease was stable in the presence of Triton X-100, isopropanol, ethanol or dithio-bis-nitrobenzoic (DTNB), but was inhibited by sodium dodecyl sulfate (SDS), dimethyl sulfoxide (DMSO) or methanol. MALDI -TOF/TOF MS analysis revealed that the protease shared some functional traits with protease produced by Halogranum salarium. Furthermore, it exhibited high hydrolytic activity on silver carp myosin protein.; Conclusion: The protease is an alkaline and salt-tolerant enzyme that hydrolyses silver carp myosin with high efficiency. These excellent characteristics make this protease an attractive candidate for industrial use in low-salt fish sauce fermentation. © 2016 Society of Chemical Industry.; © 2016 Society of Chemical Industry.
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halopelagius fulvigenes sp nov a Halophilic Archaeon isolated from a lake
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Heng-lin Cui, Bingbing Liu, Shukun Tang, Yongguang Zhang, Yuanming Zhang, Lili ZhangAbstract:Two extremely Halophilic archaea, designated YIM 94188T and YIM 94189, were isolated from Qijiaojing lake in Xinjiang province, north-west China and subjected to taxonomic characterization using a polyphasic approach. The cells of the two strains were coccoid, non-motile and Gram-stain-negative. Colonies were pink–white-pigmented and aerobic. Growth occurred at 10–30 % (w/v) NaCl, 20–55 °C and pH 6.0–8.0 (optimum: 20–25 % NaCl, 37–42 °C, pH 6.5–7.0). Magnesium was necessary for growth in the range of 0.2–1.2 M. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the two strains belonged to the genus Halopelagius showing 98.5 % sequence similarity to the closest phylogenetic neighbour, Halopelagius inordinatus RO5-2T. In addition, the DNA–DNA hybridization values of strains YIM 94188T and YIM 94189 to Halopelagius inordinatus RO5-2T were 35.7 % and 37.7 %, respectively. Polar lipid analyses revealed that the two strains contained phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1). The DNA G+C contents of strains YIM 94188T and YIM 94189 were 66.3 mol% and 64.6 mol%, respectively. On the basis of physiological and chemotaxonomic data, and phylogenetic analysis, strains YIM 94188T and YIM 94189 were classified as representing a novel species in the genus Halopelagius . The name Halopelagius fulvigenes sp. nov. is proposed, with YIM 94188T ( = CCTCC AB 2010456T = JCM 17506T) as the type strain.
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halosarcina limi sp nov a Halophilic Archaeon from a marine solar saltern and emended description of the genus halosarcina
International Journal of Systematic and Evolutionary Microbiology, 2010Co-Authors: Heng-lin Cui, Hongcan Liu, Xia Gao, Yuguang Zhou, Pei-jin ZhouAbstract:A Halophilic Archaeon, strain RO1-6T, was isolated from a marine solar saltern in eastern China. Cells of strain RO1-6T were pleomorphic and motile and stained Gram-negative. Strain RO1-6T grew well on complex medium and colonies were red-pigmented. It was able to grow at 20–50 °C (optimum 37 °C), in 2.1–5.1 M NaCl (optimum 3.9 M NaCl), in 0.05–0.70 M MgCl2 (optimum 0.30 M MgCl2) and at pH 6.5–8.0 (optimum pH 7.0). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of strain RO1-6T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two glycolipids that were chromatographically identical to S-DGD-1 and S2-DGD. The 16S rRNA gene sequence of strain RO1-6T showed similarities of 96.9 and 96.4 % to those of the type strains of Halosarcina pallida and Halogeometricum borinquense, respectively, members of the most closely related recognized genera within the family Halobacteriaceae. The DNA G+C content of strain RO1-6T was 61.2 mol%. Phenotypic characterization and phylogenetic analysis revealed that strain RO1-6T is related to Halosarcina pallida and represents a novel species of the genus Halosarcina, for which the name Halosarcina limi sp. nov. is proposed; the type strain is RO1-6T (=CGMCC 1.8711T =JCM 16054T).
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haloplanus vescus sp nov an extremely Halophilic Archaeon from a marine solar saltern and emended description of the genus haloplanus
International Journal of Systematic and Evolutionary Microbiology, 2010Co-Authors: Heng-lin Cui, Hongcan Liu, Xia Gao, Yuguang Zhou, Pei-jin ZhouAbstract:An extremely Halophilic Archaeon, strain RO5-8T, was isolated from a disused marine solar saltern in China. The cells were pleomorphic and flat. In static liquid medium, cells floated to the surface. Strain RO5-8T stained Gram-negative and colonies were pink-pigmented. It was able to grow at 30–50 °C (optimum 40 °C), at 2.6–4.3 M NaCl (optimum 3.1 M NaCl), at 0.03–0.5 M MgCl2 (optimum 0.03 M MgCl2) and at pH 5.5–7.5 (optimum pH 6.0–6.5). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of strain RO5-8T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and one major glycolipid chromatographically identical to the sulfated mannosyl glucosyl diether S-DGD-1. On the basis of 16S rRNA gene sequence analysis, strain RO5-8T was closely related to three strains of Haloplanus natans with similarities of 97.3–97.6 %. The DNA G+C content of strain RO5-8T was 62.1 mol%. The DNA–DNA hybridization value between strain RO5-8T and Haloplanus natans JCM 14081T was 51.6 %. It was concluded that strain RO5-8T represents a novel species of the genus Haloplanus, for which the name Haloplanus vescus sp. nov. is proposed. The type strain is RO5-8T (=CGMCC 1.8712T =JCM 16055T).
Frank Siedler - One of the best experts on this subject based on the ideXlab platform.
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the protein interaction network of a taxis signal transduction system in a Halophilic Archaeon
BMC Microbiology, 2012Co-Authors: Matthias Schlesner, Huseyin Besir, Michalis Aivaliotis, Judith Streif, Beatrix Scheffer, Arthur Miller, Frank SiedlerAbstract:Background The taxis signaling system of the extreme Halophilic Archaeon Halobacterium (Hbt.) salinarum differs in several aspects from its model bacterial counterparts Escherichia coli and Bacillus subtilis. We studied the protein interactions in the Hbt. salinarum taxis signaling system to gain an understanding of its structure, to gain knowledge about its known components and to search for new members.
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the protein interaction network of a taxis signal transduction system in a Halophilic Archaeon
BMC Microbiology, 2012Co-Authors: Matthias Schlesner, Huseyin Besir, Michalis Aivaliotis, Judith Streif, Beatrix Scheffer, Arthur Miller, Frank SiedlerAbstract:The taxis signaling system of the extreme Halophilic Archaeon Halobacterium (Hbt.) salinarum differs in several aspects from its model bacterial counterparts Escherichia coli and Bacillus subtilis. We studied the protein interactions in the Hbt. salinarum taxis signaling system to gain an understanding of its structure, to gain knowledge about its known components and to search for new members. The interaction analysis revealed that the core signaling proteins are involved in different protein complexes and our data provide evidence for dynamic interchanges between them. Fifteen of the eighteen taxis receptors (halobacterial transducers, Htrs) can be assigned to four different groups depending on their interactions with the core signaling proteins. Only one of these groups, which contains six of the eight Htrs with known signals, shows the composition expected for signaling complexes (receptor, kinase CheA, adaptor CheW, response regulator CheY). From the two Hbt. salinarum CheW proteins, only CheW1 is engaged in signaling complexes with Htrs and CheA, whereas CheW2 interacts with Htrs but not with CheA. CheY connects the core signaling structure to a subnetwork consisting of the two CheF proteins (which build a link to the flagellar apparatus), CheD (the hub of the subnetwork), two CheC complexes and the receptor methylesterase CheB. Based on our findings, we propose two hypotheses. First, Hbt. salinarum might have the capability to dynamically adjust the impact of certain Htrs or Htr clusters depending on its current needs or environmental conditions. Secondly, we propose a hypothetical feedback loop from the response regulator to Htr methylation made from the CheC proteins, CheD and CheB, which might contribute to adaptation analogous to the CheC/CheD system of B. subtilis.
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life style changes of a Halophilic Archaeon analyzed by quantitative proteomics
Proteomics, 2009Co-Authors: Andreas Tebbe, Michalis Aivaliotis, Frank Siedler, Alexander Schmidt, Kosta Konstantinidis, Michaela Falb, Birgit Bisle, Christian Klein, Josef Kellermann, Friedhelm PfeifferAbstract:Quantitative proteomics based on isotopic labeling has become the method of choice to accurately determine changes in protein abundance in highly complex mixtures. Isotope-coded protein labeling (ICPL), which is based on the nicotinoylation of proteins at lysine residues and free N-termini was used as a simple, reliable and fast method for the comparative analysis of three different cellular states of the Halophilic Archaeon Halobacterium salinarum through pairwise comparison. The labeled proteins were subjected to SDS-PAGE, in-gel digested and the proteolytic peptides were separated by LC and analyzed by MALDI-TOF/TOF MS. Automated quantitation was performed by comparing the MS peptide signals of (12)C and (13)C nicotinoylated isotopic peptide pairs. The transitions between (i) aerobic growth in complex versus synthetic medium and (ii) aerobic versus anaerobic/phototrophic growth, both in complex medium, provide a wide span in nutrient and energy supply for the cell and thus allowed optimal studies of proteome changes. In these two studies, 559 and 643 proteins, respectively, could be quantified allowing a detailed analysis of the adaptation of H. salinarum to changes of its living conditions. The subtle cellular response to a wide variation of nutrient and energy supply demonstrates a fine tuning of the cellular protein inventory.
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quantitative profiling of the membrane proteome in a Halophilic Archaeon
Molecular & Cellular Proteomics, 2006Co-Authors: Birgit Bisle, Frank Siedler, Friedhelm Pfeiffer, Andreas Tebbe, Alexander Schmidt, Christian Klein, Josef Kellermann, Burghardt Scheibe, Friedrich Lottspeich, Dieter OesterheltAbstract:We present a large scale quantitation study of the membrane proteome from Halobacterium salinarum. To overcome problems generally encountered with membrane proteins, we established a membrane preparation protocol that allows the application of most proteomic techniques originally developed for soluble proteins. Proteins were quantified using two complementary approaches. For gel-based quantitation, DIGE labeling was combined with two-dimensional gel electrophoresis on an improved 16-benzyldimethyl-n-hexadecylammonium chloride/SDS system. MS-based quantitation was carried out by combining gel-free separation with the recently developed isotope-coded protein labeling technique. Good correlations between these two independent quantitation strategies were obtained. From computational analysis we conclude that labeling of free amino groups by isotope-coded protein labeling (Lys and free N termini) is better suited for membrane proteins than Cys-based labeling strategies but that quantitation of integral membrane proteins remains cumbersome compared with soluble proteins. Nevertheless we could quantify 155 membrane proteins; 101 of these had transmembrane domains. We compared two growth states that strongly affect the energy supply of the cells: aerobic versus anaerobic/phototrophic conditions. The photosynthetic protein bacteriorhodopsin is the most highly regulated protein. As expected, several other membrane proteins involved in aerobic or anaerobic energy metabolism were found to be regulated, but in total, however, the number of regulated proteins is rather small.
Matthias Schlesner - One of the best experts on this subject based on the ideXlab platform.
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the protein interaction network of a taxis signal transduction system in a Halophilic Archaeon
BMC Microbiology, 2012Co-Authors: Matthias Schlesner, Huseyin Besir, Michalis Aivaliotis, Judith Streif, Beatrix Scheffer, Arthur Miller, Frank SiedlerAbstract:Background The taxis signaling system of the extreme Halophilic Archaeon Halobacterium (Hbt.) salinarum differs in several aspects from its model bacterial counterparts Escherichia coli and Bacillus subtilis. We studied the protein interactions in the Hbt. salinarum taxis signaling system to gain an understanding of its structure, to gain knowledge about its known components and to search for new members.
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the protein interaction network of a taxis signal transduction system in a Halophilic Archaeon
BMC Microbiology, 2012Co-Authors: Matthias Schlesner, Huseyin Besir, Michalis Aivaliotis, Judith Streif, Beatrix Scheffer, Arthur Miller, Frank SiedlerAbstract:The taxis signaling system of the extreme Halophilic Archaeon Halobacterium (Hbt.) salinarum differs in several aspects from its model bacterial counterparts Escherichia coli and Bacillus subtilis. We studied the protein interactions in the Hbt. salinarum taxis signaling system to gain an understanding of its structure, to gain knowledge about its known components and to search for new members. The interaction analysis revealed that the core signaling proteins are involved in different protein complexes and our data provide evidence for dynamic interchanges between them. Fifteen of the eighteen taxis receptors (halobacterial transducers, Htrs) can be assigned to four different groups depending on their interactions with the core signaling proteins. Only one of these groups, which contains six of the eight Htrs with known signals, shows the composition expected for signaling complexes (receptor, kinase CheA, adaptor CheW, response regulator CheY). From the two Hbt. salinarum CheW proteins, only CheW1 is engaged in signaling complexes with Htrs and CheA, whereas CheW2 interacts with Htrs but not with CheA. CheY connects the core signaling structure to a subnetwork consisting of the two CheF proteins (which build a link to the flagellar apparatus), CheD (the hub of the subnetwork), two CheC complexes and the receptor methylesterase CheB. Based on our findings, we propose two hypotheses. First, Hbt. salinarum might have the capability to dynamically adjust the impact of certain Htrs or Htr clusters depending on its current needs or environmental conditions. Secondly, we propose a hypothetical feedback loop from the response regulator to Htr methylation made from the CheC proteins, CheD and CheB, which might contribute to adaptation analogous to the CheC/CheD system of B. subtilis.
Pei-jin Zhou - One of the best experts on this subject based on the ideXlab platform.
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halosarcina limi sp nov a Halophilic Archaeon from a marine solar saltern and emended description of the genus halosarcina
International Journal of Systematic and Evolutionary Microbiology, 2010Co-Authors: Heng-lin Cui, Hongcan Liu, Xia Gao, Yuguang Zhou, Pei-jin ZhouAbstract:A Halophilic Archaeon, strain RO1-6T, was isolated from a marine solar saltern in eastern China. Cells of strain RO1-6T were pleomorphic and motile and stained Gram-negative. Strain RO1-6T grew well on complex medium and colonies were red-pigmented. It was able to grow at 20–50 °C (optimum 37 °C), in 2.1–5.1 M NaCl (optimum 3.9 M NaCl), in 0.05–0.70 M MgCl2 (optimum 0.30 M MgCl2) and at pH 6.5–8.0 (optimum pH 7.0). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of strain RO1-6T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two glycolipids that were chromatographically identical to S-DGD-1 and S2-DGD. The 16S rRNA gene sequence of strain RO1-6T showed similarities of 96.9 and 96.4 % to those of the type strains of Halosarcina pallida and Halogeometricum borinquense, respectively, members of the most closely related recognized genera within the family Halobacteriaceae. The DNA G+C content of strain RO1-6T was 61.2 mol%. Phenotypic characterization and phylogenetic analysis revealed that strain RO1-6T is related to Halosarcina pallida and represents a novel species of the genus Halosarcina, for which the name Halosarcina limi sp. nov. is proposed; the type strain is RO1-6T (=CGMCC 1.8711T =JCM 16054T).
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haloplanus vescus sp nov an extremely Halophilic Archaeon from a marine solar saltern and emended description of the genus haloplanus
International Journal of Systematic and Evolutionary Microbiology, 2010Co-Authors: Heng-lin Cui, Hongcan Liu, Xia Gao, Yuguang Zhou, Pei-jin ZhouAbstract:An extremely Halophilic Archaeon, strain RO5-8T, was isolated from a disused marine solar saltern in China. The cells were pleomorphic and flat. In static liquid medium, cells floated to the surface. Strain RO5-8T stained Gram-negative and colonies were pink-pigmented. It was able to grow at 30–50 °C (optimum 40 °C), at 2.6–4.3 M NaCl (optimum 3.1 M NaCl), at 0.03–0.5 M MgCl2 (optimum 0.03 M MgCl2) and at pH 5.5–7.5 (optimum pH 6.0–6.5). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of strain RO5-8T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and one major glycolipid chromatographically identical to the sulfated mannosyl glucosyl diether S-DGD-1. On the basis of 16S rRNA gene sequence analysis, strain RO5-8T was closely related to three strains of Haloplanus natans with similarities of 97.3–97.6 %. The DNA G+C content of strain RO5-8T was 62.1 mol%. The DNA–DNA hybridization value between strain RO5-8T and Haloplanus natans JCM 14081T was 51.6 %. It was concluded that strain RO5-8T represents a novel species of the genus Haloplanus, for which the name Haloplanus vescus sp. nov. is proposed. The type strain is RO5-8T (=CGMCC 1.8712T =JCM 16055T).
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Halorubrum litoreum sp. nov., an extremely Halophilic Archaeon from a solar saltern.
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Heng-lin Cui, Pei-jin Zhou, Ze-ying Lin, Ying Dong, Shuang-jiang LiuAbstract:An extremely Halophilic Archaeon, strain Fa-1(T), was isolated from a marine solar saltern in Fujian, China. Strain Fa-1(T) required Mg2+ and at least 2.0 M NaCl for growth. It was able to grow at pH 6.5-9.0 (optimally at pH 7.0-7.5) and at 20-55 degrees C (optimally at 37-42 degrees C). The major polar lipids of strain Fa-1(T) were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and a sulfated diglycosyl diether. On the basis of a 16S rRNA gene sequence analysis, strain Fa-1(T) was closely related to nine species of the genus Halorubrum, showing sequence similarities of 97.4-98.4 %. The G+C content of the DNA of strain Fa-1(T) is 64.9 mol% (Tm). DNA-DNA hybridization values between strain Fa-1(T) and the most closely related members of the genus Halorubrum were below 51 %. On the basis of the data from this study, strain Fa-1(T) represents a novel species of the genus Halorubrum, for which the name Halorubrum litoreum sp. nov. is proposed. The type strain is Fa-1(T) (=CGMCC 1.5336(T) =JCM 13561(T)).
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haloarcula amylolytica sp nov an extremely Halophilic Archaeon isolated from aibi salt lake in xin jiang china
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Yong Yang, Pei-jin Zhou, Heng-lin Cui, Shuang-jiang LiuAbstract:A starch-hydrolysing and extremely Halophilic Archaeon (strain BD-3(T)), isolated from Aibi salt lake in Xin-Jiang, China, was characterized phenotypically and genotypically in order to determine its taxonomic status. On the basis of its polar lipid composition, nucleotide sequences of its 16S rRNA genes, genomic DNA G+C content (62.4 mol%) and growth characteristics, the organism could be assigned to the genus Haloarcula. Phenotypic differences and low DNA-DNA hybridization values to related Haloarcula species distinguished strain BD-3(T) from recognized Haloarcula species. It is therefore concluded that strain BD-3(T) represents a novel species, for which the name Haloarcula amylolytica sp. nov. is proposed. The type strain is BD-3(T) (=CGMCC 1.5335(T)=JCM 13557(T)).
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halobacterium jilantaiense sp nov a Halophilic Archaeon isolated from a saline lake in inner mongolia china
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Yong Yang, Pei-jin ZhouAbstract:A novel Halophilic Archaeon, NG4T, was isolated from Jilantai salt lake in Inner Mongolia, China. The taxonomy of strain NG4T was studied by polyphasic methods. Strain NG4T grew at pH 5.5–8.5 and at a temperature of 22–55 °C. It was chemo-organotrophic, aerobic and required concentrations of 2.7–5.2 M NaCl and 0.05–0.3 M Mg2+ for growth. Cells were Gram-negative, slender rods. Colonies on agar plates containing 25 % (w/v) total salts were red, elevated and round. According to 16S rRNA gene sequence similarity, strain NG4T was phylogenetically related to Halobacterium salinarum DSM 3754T (98.2 %) and Halobacterium noricense A1T (97.3 %). The DNA G+C content was 64.2 mol%. DNA–DNA relatedness values with Hbt. salinarum DSM 3754T and Hbt. noricense A1T were 47 and 35 %, respectively. The polar lipids of strain NG4T consisted of phosphatidylglycerol, methylated phosphatidylglycerol phosphate, phosphatidylglycerol sulfate, triglycosyl diether, sulfated triglycosyl diether and sulfated tetraglycosyl diether. It was concluded that strain NG4T represents a novel species of the genus Halobacterium, for which the name Halobacterium jilantaiense sp. nov. is proposed. The type strain is NG4T (=CGMCC 1.5337T=JCM 13558T).
