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Chedly Abdelly - One of the best experts on this subject based on the ideXlab platform.
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cakile maritima a promising model for Halophyte studies and a putative cash crop for saline agriculture
2019Co-Authors: Delphine Arbeletbonnin, Chedly Abdelly, Ibtissem Benhamedlouati, Patrick Laurenti, Karim Benhamed, Francois BouteauAbstract:Abstract Agricultural intensification necessary for global food security as well as changes in temperature and precipitation patterns expected from climate change are likely to cause further deterioration irrigated lands in various part of the world. Salinization is spreading particularly in arid and semi-arid regions and urban sprawl is forcing agricultural production into marginal areas. Many salted marginal areas could support biomass production if Halophytes able to tolerate high salinity were used. This chapter presents general data on Cakile maritima, a Halophyte, concerning its ecological characteristics, diversity and distribution, adaptation to environment and its potential uses as medicinal plant, for oilseed production or phytoremediation. In a second part we present what have been uncovered in C. maritima in term of mechanisms and physiological adaptations to salinity when compared to other plants. Cakile maritima appears worthy of attention since it meets numerous criteria for being a good genetic model of Halophyte. It has a small diploid genome, a short life cycle and produces large amount of seeds. Furthermore Cakile maritima represents a promising species owing its large geographical and ecological amplitude, its economic potential because of its ability to produce numerous secondary compounds and as an oilseed and energy crop. This renders the cultivation of this plant on salted marginal soil of practical significance in the context of the necessary development of biosaline agriculture in the future.
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physiological and antioxidant responses of the sabkha biotope Halophyte limonium delicatulum to seasonal changes in environmental conditions
Plant Physiology and Biochemistry, 2018Co-Authors: Aymen Souid, Chedly Abdelly, Abderrazak Smaoui, Walid Zorrig, Lorenza Bellani, Christian Magne, Vincenzo Longo, Karim Ben HamedAbstract:Abstract In saline biotopes, different mechanisms may contribute to the tolerance of Halophytes to high soil salinity and temperature, drought and other abiotic stresses, but their relative responses and their ecological plasticity for a given species remain unknown. In this study, we examined the responses of the salt marsh Halophyte Limonium delicatulum to changing environmental conditions of its natural habitats (Sabkha “El Kalbia”, Tunisia). The specific aim of the work was to check whether statistically significant correlations could be established between particular stress response mechanisms (ion uptake, activation of antioxidant systems) and soil parameters and climatic data associated with environmental stress. The results showed that the salinity of the soil increased during the months of June, July and August (dry period), concomitantly with a strong accumulation of salt ions in the aerial parts of the plant. Moreover, the highest antioxidant capacity of this Halophyte (enzymes and antioxidant molecules) was reached during the same period of the year. The remaining periods (corresponding to the rainy season) did not show a significant difference in enzymes activities and level of antioxidants, with a minimum observed in the months of January and February. These results show a remarkable effect of salinity in the natural habitat on the activity of enzymes and antioxidant molecules. Other edaphic and climatic factors could also be involved to increase antioxidant capacity, such as nutrient deficiency, temperature and precipitation.
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Quadrupole time-of-flight mass spectrometry analysis of glycerophospholipid molecular species in the two Halophyte seed oils: Eryngium maritimum and Cakile maritima.
Food chemistry, 2016Co-Authors: Manel Zitouni, Chedly Abdelly, Vera Wewer, Peter Dörmann, Nabil Ben YoussefAbstract:Future applications of lipids in clinical cohort studies demand detailed glycerophospholipid molecule information and the application of high-throughput lipidomics platforms. In the present work, a novel sensitive technique with high mass resolution and accuracy was applied to accomplish phospholipid analysis. Nanospray ionization quadrupole time-of-flight mass spectrometry was used to separate and quantify the glycerophospholipid classes as well as molecular species in two Halophyte seed oils from Cakile maritima and Eryngium maritimum. Precursor or neutral loss scans of their polar head groups allowed the detection of molecular species within particular glycerophospholipid classes. Phosphatidylcholine was found to be the most abundant glycerophospholipid in both seed oils whereas phosphatidylethanolamine and phosphatidic acid were less abundant. Phosphatidylinositol, phosphatidylserine and phosphatidylglycerol were minor glycerophospholipids. Several molecular species within each class were detected and the main molecular species (C36:4, C36:3, C36:2, 34:2 and C34:1) were quantitatively different between the two Halophytes and the different glycerophospholipids.
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Moderate salinity reduced phenanthrene-induced stress in the Halophyte plant model Thellungiella salsuginea compared to its glycophyte relative Arabidopsis thaliana: Cross talk and metabolite profiling
Chemosphere, 2016Co-Authors: Moez Shiri, Chedly Abdelly, Mokded Rabhi, Alain Bouchereau, Abdelhak El AmraniAbstract:It was shown that Halophytes experience higher cross-tolerance to stresses than glycophytes, which was often associated with their more powerful antioxidant systems. Moreover, salinity was reported to enhance Halophyte tolerance to several stresses. The aim of the present work was to investigate whether a moderate salinity enhances phenanthrene stress tolerance in the Halophyte Thellungiella salsuginea. The model plant Arabidopsis thaliana, considered as its glycophyte relative, was used as reference. Our study was based on morpho-physiological, antioxidant, and metabolomic parameters. Results showed that T. salsuginea was more tolerant to phenanthrene stress as compared to A. thaliana. An improvement of phenanthrene-induced responses was recorded in the two plants in the presence of 25 mM NaCl, but the effect was significantly more obvious in the Halophyte. This observation was particularly related to the higher antioxidant activities and the induction of more adapted metabolism in the Halophyte. Gas Chromatography coupled with Mass Spectrometry (GC-MS) was used to quantify alcohols, ammonium, sugars, and organic acids. It showed the accumulation of several metabolites, many of them are known to be involved in signaling and abiotic stress tolerance. Moderate salinity and phenanthrene cross-tolerance involved in these two stresses was discussed
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insights into the ecology and the salt tolerance of the Halophyte cakile maritima using multidisciplinary approaches
Halophytes for Food Security in Dry Lands, 2016Co-Authors: Karim Ben Hamed, Francois Bouteau, Ibtissem Ben Hamad, Chedly AbdellyAbstract:We review information on the Halophyte Cakile maritima, member of the Brassicaceae family and native of coastal ecosystems. The first investigations focused on the ecology of the species and demonstrated latitudinal range, dispersal and environmental adaptation that make Cakile an ideal model system in which to study phenological adaptation to climate. Cakile maritima can be used as a model plant to learn about plant reactions that are not observed or experimentally reproduced in traditional glycophytic models. Ion homeostasis, osmotic adjustment, and antioxidant protection are the most studied mechanisms of salt tolerance in this species. By contrasting stress responses of C. maritima to those of stress-sensitive models such as Arabidopsis, we are convinced that differences between both plant types lie mainly in the mechanisms that control how stress signals are perceived, transduced and how adaptive processes are controlled within the plant. The establishment of cell suspension culture in C. maritima will help to evaluate salt tolerance at the cellular level. Preliminary results showed that cells exhibited similar growth and ion response to those of Halophytes.
Pritish Kumar Varadwaj - One of the best experts on this subject based on the ideXlab platform.
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Primary responses to salt stress in a Halophyte, smooth cordgrass (Spartina alterniflora Loisel.)
Functional & Integrative Genomics, 2008Co-Authors: Niranjan Baisakh, Prasanta K Subudhi, Pritish Kumar VaradwajAbstract:The response of a grass Halophyte Spartina alterniflora at early stages of salt stress was investigated through generation and systematic analysis of expressed sequence tags (ESTs) from both leaf and root tissues. Random EST sequencing produced 1,227 quality ESTs, which were clustered into 127 contigs, and 368 were singletons. Of the 495 unigenes, 27% represented genes for stress response. Comparison of the 368 singletons against the Oryza sativa gene index showed that >85% of these genes had similarity with the rice unigenes. Moreover, the phylogenetic analysis of an EST similar to myo -inositol 1-phosphate synthase of Spartina and some selected grasses and Halophytes showed closeness of Spartina with maize and rice. Transcript abundance analysis involving eight known genes of various metabolic pathways and nine transcription factor genes showed temporal and tissue-dependent variation in expression under salinity. Reverse northern analysis of a few selected unknown and ribosomal genes exhibited much higher abundance of transcripts in response to salt stress. The results provide evidence that, in addition to several unknown genes discovered in this study, genes involved in ion transport, osmolyte production, and house-keeping functions may play an important role in the primary responses to salt stress in this grass Halophyte.
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primary responses to salt stress in a Halophyte smooth cordgrass spartina alterniflora loisel
Functional & Integrative Genomics, 2008Co-Authors: Niranjan Baisakh, Prasanta K Subudhi, Pritish Kumar VaradwajAbstract:The response of a grass Halophyte Spartina alterniflora at early stages of salt stress was investigated through generation and systematic analysis of expressed sequence tags (ESTs) from both leaf and root tissues. Random EST sequencing produced 1,227 quality ESTs, which were clustered into 127 contigs, and 368 were singletons. Of the 495 unigenes, 27% represented genes for stress response. Comparison of the 368 singletons against the Oryza sativa gene index showed that >85% of these genes had similarity with the rice unigenes. Moreover, the phylogenetic analysis of an EST similar to myo-inositol 1-phosphate synthase of Spartina and some selected grasses and Halophytes showed closeness of Spartina with maize and rice. Transcript abundance analysis involving eight known genes of various metabolic pathways and nine transcription factor genes showed temporal and tissue-dependent variation in expression under salinity. Reverse northern analysis of a few selected unknown and ribosomal genes exhibited much higher abundance of transcripts in response to salt stress. The results provide evidence that, in addition to several unknown genes discovered in this study, genes involved in ion transport, osmolyte production, and house-keeping functions may play an important role in the primary responses to salt stress in this grass Halophyte.
Sheng Qin - One of the best experts on this subject based on the ideXlab platform.
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root exudates driven rhizosphere recruitment of the plant growth promoting rhizobacterium bacillus flexus klbmp 4941 and its growth promoting effect on the coastal Halophyte limonium sinense under salt stress
Ecotoxicology and Environmental Safety, 2020Co-Authors: Youwei Xiong, Ke Xing, Tiantian Wang, Yuan Gong, Chunmei Zhang, Sheng QinAbstract:Halophytes play an important role in the bioremediation of saline soils. Increased evidence has revealed that plant growth-promoting rhizobacteria (PGPR) have colonized the halophytic rhizosphere, and they have evolved the capacity to reduce salt stress damage to the host. However, the mechanism by which Halophytes attract and recruit beneficial PGPR has rarely been reported. This study reports the interaction between the Halophyte Limonium sinense and its rhizosphere PGPR strain Bacillus flexus KLBMP 4941, as well as the mechanism by which KLBMP 4941 promotes host plant growth under salt stress. After salt stress treatment, we collected the root exudates (REs) of L. sinense and found that the REs could promote the growth and chemotaxis of the bacterium KLBMP 4941. In addition, the components of the REs under salt stress were analyzed, and some organic acids (2-methylbutyric acid, stearic acid, palmitic acid, palmitoleic acid, and oleic acid) were detected as the major components. Further assessment showed that each of these components had positive effects on the growth, motility, chemotaxis, and root colonization of strain KLBMP 4941. Further pot experiments revealed the potential PGP mechanisms induced by strain KLBMP 4941 on the host plant under salt stress. Inoculation with KLBMP 4941 promoted the accumulation of chlorophyll to enhance photosynthesis, increased osmotic regulator contents, enhanced flavonoid and antioxidant enzymes, and regulated Na+/K+ homeostasis to help the host ameliorate salinity stress damage. Our findings indicate that the Halophyte L. sinense can attract and recruit beneficial rhizosphere bacteria by REs under salt stress, and the recruited B. flexus KLBMP 4941 elicited PGP effects under salinity stress through complex plant physiological regulatory mechanisms. This study provides a foundation for the enhancement of the rhizosphere colonization ability of the PGP strain KLBMP 4941, which shows potential applications in phytoremediation of saline soils.
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diversity of bacterial microbiota of coastal Halophyte limonium sinense and amelioration of salinity stress damage by symbiotic plant growth promoting actinobacterium glutamicibacter halophytocola klbmp 5180
Applied and Environmental Microbiology, 2018Co-Authors: Sheng Qin, Yueji Zhang, Youwei Xiong, Tiantian Wang, Weiwei Feng, Ke XingAbstract:ABSTRACT Plant-associated microorganisms are considered a key determinant of plant health and growth. However, little information is available regarding the composition and ecological function of the roots9 and leaves9 bacterial microbiota of Halophytes. Here, using both culture-dependent and culture-independent techniques, we characterized the bacterial communities of the roots and leaves as well as the rhizosphere and bulk soils of the coastal Halophyte Limonium sinense in Jiangsu Province, China. We identified 49 representative bacterial strains belonging to 17 genera across all samples, with Glutamicibacter as the most dominant genus. All Glutamicibacter isolates showed multiple potential plant growth promotion traits and tolerated a high concentration of NaCl and a wide pH range. Interestingly, further inoculation experiments showed that the Glutamicibacter halophytocola strain KLBMP 5180 isolated from root tissue significantly promoted host growth under NaCl stress. Indeed, KLBMP 5180 inoculation increased the concentrations of total chlorophyll, proline, antioxidative enzymes, flavonoids, K+, and Ca2+ in the leaves; the concentrations of malondialdehyde (MDA) and Na+ were reduced. A transcriptome analysis identified 1,359 and 328 differentially expressed genes (DEGs) in inoculated seedlings treated with 0 and 250 mM NaCl, respectively. We found that pathways related to phenylpropanoid and flavonoid biosynthesis and ion transport and metabolism might play more important roles in host salt stress tolerance induced by KLBMP 5180 inoculation compared to that in noninoculated leaves. Our results provide novel insights into the complex composition and function of the bacterial microbiota of the coastal Halophyte L. sinense and suggest that Halophytes might recruit specific bacteria to enhance their tolerance of harsh environments. IMPORTANCE Halophytes are important coastal plants often used for the remediation of saline coastal soils. Limonium sinense is well known for its medical properties and phytoremediation of saline soils. However, excessive exploitation and utilization have made the wild resource endangered. The use of endophytic and rhizosphere bacteria may be one of the suitable ways to solve the problem. This study was undertaken to develop approaches to improve the growth of L. sinense using endophytes. The application of actinobacterial endophytes ameliorated salt stress damage of the host via complex physiological and molecular mechanisms. The results also highlight the potential of using habitat-adapted, symbiotic, indigenous endophytic bacteria to enhance the growth and ameliorate abiotic stress damage of host plants growing in special habitats.
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isolation of acc deaminase producing habitat adapted symbiotic bacteria associated with Halophyte limonium sinense girard kuntze and evaluating their plant growth promoting activity under salt stress
Plant and Soil, 2014Co-Authors: Sheng Qin, Yueji Zhang, Bo Yuan, Ke Xing, Jun Wang, Jihong JiangAbstract:Background and aims Many plant growth-promoting endophytes (PGPE) possessing 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity can reduce the level of stress ethylene and assist their host plants cope with various biotic and abiotic stresses. However, information about the endophytic bacteria colonizing in the coastal Halophytes is still very scarce. This study aims at isolating efficient ACC deaminase-producing plant growth-promoting (PGP) bacterial strains from the inner tissues of a traditional Chinese folk medicine Limonium sinense (Girard) Kuntze, a Halophyte which has high economic and medicinal values grown in the coastal saline soils. Their PGP activity and effects on host seed germination and seedling growth under salinity stress were also evaluated.
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isolation of acc deaminase producing habitat adapted symbiotic bacteria associated with Halophyte limonium sinense girard kuntze and evaluating their plant growth promoting activity under salt stress
Plant and Soil, 2014Co-Authors: Sheng Qin, Yueji Zhang, Bo Yuan, Ke Xing, Jun Wang, Jihong JiangAbstract:Many plant growth-promoting endophytes (PGPE) possessing 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity can reduce the level of stress ethylene and assist their host plants cope with various biotic and abiotic stresses. However, information about the endophytic bacteria colonizing in the coastal Halophytes is still very scarce. This study aims at isolating efficient ACC deaminase-producing plant growth-promoting (PGP) bacterial strains from the inner tissues of a traditional Chinese folk medicine Limonium sinense (Girard) Kuntze, a Halophyte which has high economic and medicinal values grown in the coastal saline soils. Their PGP activity and effects on host seed germination and seedling growth under salinity stress were also evaluated. A total of 126 isolates were obtained from the surface sterilized roots, stems and leaves of L. sinense (Girard) Kuntze. They were initially selected for their ability to produce ACC deaminase as well as other PGP properties such as production of indole-3-acetic acid (IAA), N2-fixation, and phosphate-solubilizing activities and subsequently identified by the 16S rRNA gene sequencing. For selected strains, seed germination, seedling growth, and flavonoids production in axenically growth L. sinense (Girard) Kuntze seedlings at different NaCl concentrations (0–500 mM) were quantified. Thirteen isolates possessing ACC deaminase activity were obtained. The 16S rRNA gene sequencing analysis showed them to belong to eight genera: Bacillus, Pseudomonas, Klebsiella, Serratia, Arthrobacter, Streptomyces, Isoptericola, and Microbacterium. Inoculation with four of the selected ACC deaminase-producing strains not only stimulated the growth of the host plant but also influenced the flavonoids accumulation. All four strains could colonize and can be re-isolated from the host plant interior tissues. These results demonstrate that ACC deaminase-producing habitat-adapted symbiotic bacteria isolated from Halophyte could enhance plant growth under saline stress conditions and the PGPE strains could be appropriate as bioinoculants to enhance soil fertility and protect the plants against salt stress.
Ke Xing - One of the best experts on this subject based on the ideXlab platform.
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root exudates driven rhizosphere recruitment of the plant growth promoting rhizobacterium bacillus flexus klbmp 4941 and its growth promoting effect on the coastal Halophyte limonium sinense under salt stress
Ecotoxicology and Environmental Safety, 2020Co-Authors: Youwei Xiong, Ke Xing, Tiantian Wang, Yuan Gong, Chunmei Zhang, Sheng QinAbstract:Halophytes play an important role in the bioremediation of saline soils. Increased evidence has revealed that plant growth-promoting rhizobacteria (PGPR) have colonized the halophytic rhizosphere, and they have evolved the capacity to reduce salt stress damage to the host. However, the mechanism by which Halophytes attract and recruit beneficial PGPR has rarely been reported. This study reports the interaction between the Halophyte Limonium sinense and its rhizosphere PGPR strain Bacillus flexus KLBMP 4941, as well as the mechanism by which KLBMP 4941 promotes host plant growth under salt stress. After salt stress treatment, we collected the root exudates (REs) of L. sinense and found that the REs could promote the growth and chemotaxis of the bacterium KLBMP 4941. In addition, the components of the REs under salt stress were analyzed, and some organic acids (2-methylbutyric acid, stearic acid, palmitic acid, palmitoleic acid, and oleic acid) were detected as the major components. Further assessment showed that each of these components had positive effects on the growth, motility, chemotaxis, and root colonization of strain KLBMP 4941. Further pot experiments revealed the potential PGP mechanisms induced by strain KLBMP 4941 on the host plant under salt stress. Inoculation with KLBMP 4941 promoted the accumulation of chlorophyll to enhance photosynthesis, increased osmotic regulator contents, enhanced flavonoid and antioxidant enzymes, and regulated Na+/K+ homeostasis to help the host ameliorate salinity stress damage. Our findings indicate that the Halophyte L. sinense can attract and recruit beneficial rhizosphere bacteria by REs under salt stress, and the recruited B. flexus KLBMP 4941 elicited PGP effects under salinity stress through complex plant physiological regulatory mechanisms. This study provides a foundation for the enhancement of the rhizosphere colonization ability of the PGP strain KLBMP 4941, which shows potential applications in phytoremediation of saline soils.
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diversity of bacterial microbiota of coastal Halophyte limonium sinense and amelioration of salinity stress damage by symbiotic plant growth promoting actinobacterium glutamicibacter halophytocola klbmp 5180
Applied and Environmental Microbiology, 2018Co-Authors: Sheng Qin, Yueji Zhang, Youwei Xiong, Tiantian Wang, Weiwei Feng, Ke XingAbstract:ABSTRACT Plant-associated microorganisms are considered a key determinant of plant health and growth. However, little information is available regarding the composition and ecological function of the roots9 and leaves9 bacterial microbiota of Halophytes. Here, using both culture-dependent and culture-independent techniques, we characterized the bacterial communities of the roots and leaves as well as the rhizosphere and bulk soils of the coastal Halophyte Limonium sinense in Jiangsu Province, China. We identified 49 representative bacterial strains belonging to 17 genera across all samples, with Glutamicibacter as the most dominant genus. All Glutamicibacter isolates showed multiple potential plant growth promotion traits and tolerated a high concentration of NaCl and a wide pH range. Interestingly, further inoculation experiments showed that the Glutamicibacter halophytocola strain KLBMP 5180 isolated from root tissue significantly promoted host growth under NaCl stress. Indeed, KLBMP 5180 inoculation increased the concentrations of total chlorophyll, proline, antioxidative enzymes, flavonoids, K+, and Ca2+ in the leaves; the concentrations of malondialdehyde (MDA) and Na+ were reduced. A transcriptome analysis identified 1,359 and 328 differentially expressed genes (DEGs) in inoculated seedlings treated with 0 and 250 mM NaCl, respectively. We found that pathways related to phenylpropanoid and flavonoid biosynthesis and ion transport and metabolism might play more important roles in host salt stress tolerance induced by KLBMP 5180 inoculation compared to that in noninoculated leaves. Our results provide novel insights into the complex composition and function of the bacterial microbiota of the coastal Halophyte L. sinense and suggest that Halophytes might recruit specific bacteria to enhance their tolerance of harsh environments. IMPORTANCE Halophytes are important coastal plants often used for the remediation of saline coastal soils. Limonium sinense is well known for its medical properties and phytoremediation of saline soils. However, excessive exploitation and utilization have made the wild resource endangered. The use of endophytic and rhizosphere bacteria may be one of the suitable ways to solve the problem. This study was undertaken to develop approaches to improve the growth of L. sinense using endophytes. The application of actinobacterial endophytes ameliorated salt stress damage of the host via complex physiological and molecular mechanisms. The results also highlight the potential of using habitat-adapted, symbiotic, indigenous endophytic bacteria to enhance the growth and ameliorate abiotic stress damage of host plants growing in special habitats.
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isolation of acc deaminase producing habitat adapted symbiotic bacteria associated with Halophyte limonium sinense girard kuntze and evaluating their plant growth promoting activity under salt stress
Plant and Soil, 2014Co-Authors: Sheng Qin, Yueji Zhang, Bo Yuan, Ke Xing, Jun Wang, Jihong JiangAbstract:Background and aims Many plant growth-promoting endophytes (PGPE) possessing 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity can reduce the level of stress ethylene and assist their host plants cope with various biotic and abiotic stresses. However, information about the endophytic bacteria colonizing in the coastal Halophytes is still very scarce. This study aims at isolating efficient ACC deaminase-producing plant growth-promoting (PGP) bacterial strains from the inner tissues of a traditional Chinese folk medicine Limonium sinense (Girard) Kuntze, a Halophyte which has high economic and medicinal values grown in the coastal saline soils. Their PGP activity and effects on host seed germination and seedling growth under salinity stress were also evaluated.
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isolation of acc deaminase producing habitat adapted symbiotic bacteria associated with Halophyte limonium sinense girard kuntze and evaluating their plant growth promoting activity under salt stress
Plant and Soil, 2014Co-Authors: Sheng Qin, Yueji Zhang, Bo Yuan, Ke Xing, Jun Wang, Jihong JiangAbstract:Many plant growth-promoting endophytes (PGPE) possessing 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity can reduce the level of stress ethylene and assist their host plants cope with various biotic and abiotic stresses. However, information about the endophytic bacteria colonizing in the coastal Halophytes is still very scarce. This study aims at isolating efficient ACC deaminase-producing plant growth-promoting (PGP) bacterial strains from the inner tissues of a traditional Chinese folk medicine Limonium sinense (Girard) Kuntze, a Halophyte which has high economic and medicinal values grown in the coastal saline soils. Their PGP activity and effects on host seed germination and seedling growth under salinity stress were also evaluated. A total of 126 isolates were obtained from the surface sterilized roots, stems and leaves of L. sinense (Girard) Kuntze. They were initially selected for their ability to produce ACC deaminase as well as other PGP properties such as production of indole-3-acetic acid (IAA), N2-fixation, and phosphate-solubilizing activities and subsequently identified by the 16S rRNA gene sequencing. For selected strains, seed germination, seedling growth, and flavonoids production in axenically growth L. sinense (Girard) Kuntze seedlings at different NaCl concentrations (0–500 mM) were quantified. Thirteen isolates possessing ACC deaminase activity were obtained. The 16S rRNA gene sequencing analysis showed them to belong to eight genera: Bacillus, Pseudomonas, Klebsiella, Serratia, Arthrobacter, Streptomyces, Isoptericola, and Microbacterium. Inoculation with four of the selected ACC deaminase-producing strains not only stimulated the growth of the host plant but also influenced the flavonoids accumulation. All four strains could colonize and can be re-isolated from the host plant interior tissues. These results demonstrate that ACC deaminase-producing habitat-adapted symbiotic bacteria isolated from Halophyte could enhance plant growth under saline stress conditions and the PGPE strains could be appropriate as bioinoculants to enhance soil fertility and protect the plants against salt stress.
Brent L Nielsen - One of the best experts on this subject based on the ideXlab platform.
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transcriptome assembly profiling and differential gene expression analysis of the Halophyte suaeda fruticosa provides insights into salt tolerance
BMC Genomics, 2015Co-Authors: Joann Dirayarce, Mark J Clement, Ajmal M Khan, Brent L NielsenAbstract:Improvement of crop production is needed to feed the growing world population as the amount and quality of agricultural land decreases and soil salinity increases. This has stimulated research on salt tolerance in plants. Most crops tolerate a limited amount of salt to survive and produce biomass, while Halophytes (salt-tolerant plants) have the ability to grow with saline water utilizing specific biochemical mechanisms. However, little is known about the genes involved in salt tolerance. We have characterized the transcriptome of Suaeda fruticosa, a Halophyte that has the ability to sequester salts in its leaves. Suaeda fruticosa is an annual shrub in the family Chenopodiaceae found in coastal and inland regions of Pakistan and Mediterranean shores. This plant is an obligate Halophyte that grows optimally from 200–400 mM NaCl and can grow at up to 1000 mM NaCl. High throughput sequencing technology was performed to provide understanding of genes involved in the salt tolerance mechanism. De novo assembly of the transcriptome and analysis has allowed identification of differentially expressed and unique genes present in this non-conventional crop. Twelve sequencing libraries prepared from control (0 mM NaCl treated) and optimum (300 mM NaCl treated) plants were sequenced using Illumina Hiseq 2000 to investigate differential gene expression between shoots and roots of Suaeda fruticosa. The transcriptome was assembled de novo using Velvet and Oases k-45 and clustered using CDHIT-EST. There are 54,526 unigenes; among these 475 genes are downregulated and 44 are upregulated when samples from plants grown under optimal salt are compared with those grown without salt. BLAST analysis identified the differentially expressed genes, which were categorized in gene ontology terms and their pathways. This work has identified potential genes involved in salt tolerance in Suaeda fruticosa, and has provided an outline of tools to use for de novo transcriptome analysis. The assemblies that were used provide coverage of a considerable proportion of the transcriptome, which allows analysis of differential gene expression and identification of genes that may be involved in salt tolerance. The transcriptome may serve as a reference sequence for study of other succulent Halophytes.
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transcriptome assembly profiling and differential gene expression analysis of the Halophyte suaeda fruticosa provides insights into salt tolerance
BMC Genomics, 2015Co-Authors: Joann Dirayarce, Mark J Clement, Ajmal M Khan, Brent L NielsenAbstract:Improvement of crop production is needed to feed the growing world population as the amount and quality of agricultural land decreases and soil salinity increases. This has stimulated research on salt tolerance in plants. Most crops tolerate a limited amount of salt to survive and produce biomass, while Halophytes (salt-tolerant plants) have the ability to grow with saline water utilizing specific biochemical mechanisms. However, little is known about the genes involved in salt tolerance. We have characterized the transcriptome of Suaeda fruticosa, a Halophyte that has the ability to sequester salts in its leaves. Suaeda fruticosa is an annual shrub in the family Chenopodiaceae found in coastal and inland regions of Pakistan and Mediterranean shores. This plant is an obligate Halophyte that grows optimally from 200–400 mM NaCl and can grow at up to 1000 mM NaCl. High throughput sequencing technology was performed to provide understanding of genes involved in the salt tolerance mechanism. De novo assembly of the transcriptome and analysis has allowed identification of differentially expressed and unique genes present in this non-conventional crop. Twelve sequencing libraries prepared from control (0 mM NaCl treated) and optimum (300 mM NaCl treated) plants were sequenced using Illumina Hiseq 2000 to investigate differential gene expression between shoots and roots of Suaeda fruticosa. The transcriptome was assembled de novo using Velvet and Oases k-45 and clustered using CDHIT-EST. There are 54,526 unigenes; among these 475 genes are downregulated and 44 are upregulated when samples from plants grown under optimal salt are compared with those grown without salt. BLAST analysis identified the differentially expressed genes, which were categorized in gene ontology terms and their pathways. This work has identified potential genes involved in salt tolerance in Suaeda fruticosa, and has provided an outline of tools to use for de novo transcriptome analysis. The assemblies that were used provide coverage of a considerable proportion of the transcriptome, which allows analysis of differential gene expression and identification of genes that may be involved in salt tolerance. The transcriptome may serve as a reference sequence for study of other succulent Halophytes.
