The Experts below are selected from a list of 99 Experts worldwide ranked by ideXlab platform
Tao Wang - One of the best experts on this subject based on the ideXlab platform.
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Hantavirus like particles generated in cho cells induce specific immune responses in c57bl 6 mice
Vaccine, 2010Co-Authors: Feng Liu, Mifang Liang, Quanfu Zhang, Xiaofang Wang, Tao WangAbstract:A safe and effective Hantavirus Vaccine is highly desirable since Hantaviruses are distributed worldwide and cause an acute and often fatal disease (hemorrhagic fever with renal syndrome, HFRS). Virus-like particles (VLPs) displaying functional viral proteins could provide effective Vaccines against a few viruses, but their ability to induce Hantavirus-specific immune response has not been adequately investigated. To measure the immunogenicity of Hantaan virus-like particles (HTN-VLPs) Vaccine, we generated recombinant HTN-VLPs by co-expressing Hantaan virus nucleocapsid (N) protein and glycoproteins (Gn and Gc) in Chinese hamster ovary (CHO) cells. We compared intramuscular versus subcutaneous administration of HTN-VLPs for the ability to induce specific immune response against Hantaan virus infection. Mice that received both intramuscular and subcutaneous immunizations of HTN-VLPs were sufficiently stimulated specific antibody response against Hantaan virus N protein and glycoproteins, which was comparable to Chinese commercial inactivated bivalent Hantaviruses Vaccine. Moreover, vaccination with HTN-VLPs also resulted in the induction of higher levels of specific cellular response to N protein than that of inactivated Vaccine. Our results provide an important insight towards the development of Hantaviruses-like particles as a potential candidate Vaccine for the control and prevention of Hantaviruses infection.
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Hantavirus-like particles generated in CHO cells induce specific immune responses in C57BL/6 mice
Vaccine, 2010Co-Authors: Feng Liu, Mifang Liang, Quanfu Zhang, Xiaofang Wang, Tao WangAbstract:A safe and effective Hantavirus Vaccine is highly desirable since Hantaviruses are distributed worldwide and cause an acute and often fatal disease (hemorrhagic fever with renal syndrome, HFRS). Virus-like particles (VLPs) displaying functional viral proteins could provide effective Vaccines against a few viruses, but their ability to induce Hantavirus-specific immune response has not been adequately investigated. To measure the immunogenicity of Hantaan virus-like particles (HTN-VLPs) Vaccine, we generated recombinant HTN-VLPs by co-expressing Hantaan virus nucleocapsid (N) protein and glycoproteins (Gn and Gc) in Chinese hamster ovary (CHO) cells. We compared intramuscular versus subcutaneous administration of HTN-VLPs for the ability to induce specific immune response against Hantaan virus infection. Mice that received both intramuscular and subcutaneous immunizations of HTN-VLPs were sufficiently stimulated specific antibody response against Hantaan virus N protein and glycoproteins, which was comparable to Chinese commercial inactivated bivalent Hantaviruses Vaccine. Moreover, vaccination with HTN-VLPs also resulted in the induction of higher levels of specific cellular response to N protein than that of inactivated Vaccine. Our results provide an important insight towards the development of Hantaviruses-like particles as a potential candidate Vaccine for the control and prevention of Hantaviruses infection.
Feng Liu - One of the best experts on this subject based on the ideXlab platform.
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Hantavirus like particles generated in cho cells induce specific immune responses in c57bl 6 mice
Vaccine, 2010Co-Authors: Feng Liu, Mifang Liang, Quanfu Zhang, Xiaofang Wang, Tao WangAbstract:A safe and effective Hantavirus Vaccine is highly desirable since Hantaviruses are distributed worldwide and cause an acute and often fatal disease (hemorrhagic fever with renal syndrome, HFRS). Virus-like particles (VLPs) displaying functional viral proteins could provide effective Vaccines against a few viruses, but their ability to induce Hantavirus-specific immune response has not been adequately investigated. To measure the immunogenicity of Hantaan virus-like particles (HTN-VLPs) Vaccine, we generated recombinant HTN-VLPs by co-expressing Hantaan virus nucleocapsid (N) protein and glycoproteins (Gn and Gc) in Chinese hamster ovary (CHO) cells. We compared intramuscular versus subcutaneous administration of HTN-VLPs for the ability to induce specific immune response against Hantaan virus infection. Mice that received both intramuscular and subcutaneous immunizations of HTN-VLPs were sufficiently stimulated specific antibody response against Hantaan virus N protein and glycoproteins, which was comparable to Chinese commercial inactivated bivalent Hantaviruses Vaccine. Moreover, vaccination with HTN-VLPs also resulted in the induction of higher levels of specific cellular response to N protein than that of inactivated Vaccine. Our results provide an important insight towards the development of Hantaviruses-like particles as a potential candidate Vaccine for the control and prevention of Hantaviruses infection.
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Hantavirus-like particles generated in CHO cells induce specific immune responses in C57BL/6 mice
Vaccine, 2010Co-Authors: Feng Liu, Mifang Liang, Quanfu Zhang, Xiaofang Wang, Tao WangAbstract:A safe and effective Hantavirus Vaccine is highly desirable since Hantaviruses are distributed worldwide and cause an acute and often fatal disease (hemorrhagic fever with renal syndrome, HFRS). Virus-like particles (VLPs) displaying functional viral proteins could provide effective Vaccines against a few viruses, but their ability to induce Hantavirus-specific immune response has not been adequately investigated. To measure the immunogenicity of Hantaan virus-like particles (HTN-VLPs) Vaccine, we generated recombinant HTN-VLPs by co-expressing Hantaan virus nucleocapsid (N) protein and glycoproteins (Gn and Gc) in Chinese hamster ovary (CHO) cells. We compared intramuscular versus subcutaneous administration of HTN-VLPs for the ability to induce specific immune response against Hantaan virus infection. Mice that received both intramuscular and subcutaneous immunizations of HTN-VLPs were sufficiently stimulated specific antibody response against Hantaan virus N protein and glycoproteins, which was comparable to Chinese commercial inactivated bivalent Hantaviruses Vaccine. Moreover, vaccination with HTN-VLPs also resulted in the induction of higher levels of specific cellular response to N protein than that of inactivated Vaccine. Our results provide an important insight towards the development of Hantaviruses-like particles as a potential candidate Vaccine for the control and prevention of Hantaviruses infection.
Ziguo Yuan - One of the best experts on this subject based on the ideXlab platform.
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generation of e3 deleted canine adenovirus type 2 expressing the gc glycoprotein of seoul virus by gene insertion or deletion of related terminal region sequences
Journal of General Virology, 2010Co-Authors: Ziguo Yuan, Xiaohu Wang, Shengjun Luo, Liguo Yuan, Xiuxiang ZhangAbstract:Seoul virus (SEOV) is one of the four Hantaviruses known to cause haemorrhagic fever with renal syndrome. The medium genome segment encodes the Gn/Gc glycoproteins of SEOV, which form the major structural part of the virus envelope. Gc and/or Gn are the candidate antigens of Hantavirus for induction of a highly immunogenic response for Hantavirus Vaccine. In this study, the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the Gc protein of SEOV was evaluated in BALB/c mice. Sera from immunized mice contained neutralizing antibodies that could specifically recognize SEOV and neutralize its infectivity in vitro. Moreover, the recombinant virus induced complete protection against an intensive infectious challenge with ∼1000 50 % infective doses for SEOV strain CC-2. Protective-level neutralizing antibodies were maintained for at least 20 weeks. This recombinant virus is therefore a potential alternative to the inactivated Vaccine.
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Generation of E3-deleted canine adenovirus type 2 expressing the Gc glycoprotein of Seoul virus by gene insertion or deletion of related terminal region sequences.
The Journal of general virology, 2010Co-Authors: Ziguo Yuan, Xiaohu Wang, Shengjun Luo, Liguo Yuan, Xiuxiang ZhangAbstract:Seoul virus (SEOV) is one of the four Hantaviruses known to cause haemorrhagic fever with renal syndrome. The medium genome segment encodes the Gn/Gc glycoproteins of SEOV, which form the major structural part of the virus envelope. Gc and/or Gn are the candidate antigens of Hantavirus for induction of a highly immunogenic response for Hantavirus Vaccine. In this study, the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the Gc protein of SEOV was evaluated in BALB/c mice. Sera from immunized mice contained neutralizing antibodies that could specifically recognize SEOV and neutralize its infectivity in vitro. Moreover, the recombinant virus induced complete protection against an intensive infectious challenge with approximately 1000 50 % infective doses for SEOV strain CC-2. Protective-level neutralizing antibodies were maintained for at least 20 weeks. This recombinant virus is therefore a potential alternative to the inactivated Vaccine.
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development of recombinant canine adenovirus type 2 expressing the gn glycoprotein of seoul virus
Biologicals, 2008Co-Authors: Ziguo Yuan, Xiuxiang Zhang, Shoufeng Zhang, Ye Liu, Shengyan Gao, Fei Zhang, Xiaohu WangAbstract:Seoul virus glycoprotein Gn is a major structural protein and candidate antigen of Hantavirus that induces a highly immunogenic response for Hantavirus Vaccine. In this study, a replication-competent recombinant canine adenovirus type-2 expressing Gn was constructed by the in vitro ligation method. The Gn expression cassette, including the human cytomegalovirus (hCMV) promoter/enhancer and the SV40 early mRNA polyadenylation signal, was cloned into the SspI site of the E3 region which is not essential for proliferation of CAV-2. Expression of Gn was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting.
Xiuxiang Zhang - One of the best experts on this subject based on the ideXlab platform.
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generation of e3 deleted canine adenovirus type 2 expressing the gc glycoprotein of seoul virus by gene insertion or deletion of related terminal region sequences
Journal of General Virology, 2010Co-Authors: Ziguo Yuan, Xiaohu Wang, Shengjun Luo, Liguo Yuan, Xiuxiang ZhangAbstract:Seoul virus (SEOV) is one of the four Hantaviruses known to cause haemorrhagic fever with renal syndrome. The medium genome segment encodes the Gn/Gc glycoproteins of SEOV, which form the major structural part of the virus envelope. Gc and/or Gn are the candidate antigens of Hantavirus for induction of a highly immunogenic response for Hantavirus Vaccine. In this study, the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the Gc protein of SEOV was evaluated in BALB/c mice. Sera from immunized mice contained neutralizing antibodies that could specifically recognize SEOV and neutralize its infectivity in vitro. Moreover, the recombinant virus induced complete protection against an intensive infectious challenge with ∼1000 50 % infective doses for SEOV strain CC-2. Protective-level neutralizing antibodies were maintained for at least 20 weeks. This recombinant virus is therefore a potential alternative to the inactivated Vaccine.
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Generation of E3-deleted canine adenovirus type 2 expressing the Gc glycoprotein of Seoul virus by gene insertion or deletion of related terminal region sequences.
The Journal of general virology, 2010Co-Authors: Ziguo Yuan, Xiaohu Wang, Shengjun Luo, Liguo Yuan, Xiuxiang ZhangAbstract:Seoul virus (SEOV) is one of the four Hantaviruses known to cause haemorrhagic fever with renal syndrome. The medium genome segment encodes the Gn/Gc glycoproteins of SEOV, which form the major structural part of the virus envelope. Gc and/or Gn are the candidate antigens of Hantavirus for induction of a highly immunogenic response for Hantavirus Vaccine. In this study, the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the Gc protein of SEOV was evaluated in BALB/c mice. Sera from immunized mice contained neutralizing antibodies that could specifically recognize SEOV and neutralize its infectivity in vitro. Moreover, the recombinant virus induced complete protection against an intensive infectious challenge with approximately 1000 50 % infective doses for SEOV strain CC-2. Protective-level neutralizing antibodies were maintained for at least 20 weeks. This recombinant virus is therefore a potential alternative to the inactivated Vaccine.
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development of recombinant canine adenovirus type 2 expressing the gn glycoprotein of seoul virus
Biologicals, 2008Co-Authors: Ziguo Yuan, Xiuxiang Zhang, Shoufeng Zhang, Ye Liu, Shengyan Gao, Fei Zhang, Xiaohu WangAbstract:Seoul virus glycoprotein Gn is a major structural protein and candidate antigen of Hantavirus that induces a highly immunogenic response for Hantavirus Vaccine. In this study, a replication-competent recombinant canine adenovirus type-2 expressing Gn was constructed by the in vitro ligation method. The Gn expression cassette, including the human cytomegalovirus (hCMV) promoter/enhancer and the SV40 early mRNA polyadenylation signal, was cloned into the SspI site of the E3 region which is not essential for proliferation of CAV-2. Expression of Gn was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting.
Mifang Liang - One of the best experts on this subject based on the ideXlab platform.
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Hantavirus like particles generated in cho cells induce specific immune responses in c57bl 6 mice
Vaccine, 2010Co-Authors: Feng Liu, Mifang Liang, Quanfu Zhang, Xiaofang Wang, Tao WangAbstract:A safe and effective Hantavirus Vaccine is highly desirable since Hantaviruses are distributed worldwide and cause an acute and often fatal disease (hemorrhagic fever with renal syndrome, HFRS). Virus-like particles (VLPs) displaying functional viral proteins could provide effective Vaccines against a few viruses, but their ability to induce Hantavirus-specific immune response has not been adequately investigated. To measure the immunogenicity of Hantaan virus-like particles (HTN-VLPs) Vaccine, we generated recombinant HTN-VLPs by co-expressing Hantaan virus nucleocapsid (N) protein and glycoproteins (Gn and Gc) in Chinese hamster ovary (CHO) cells. We compared intramuscular versus subcutaneous administration of HTN-VLPs for the ability to induce specific immune response against Hantaan virus infection. Mice that received both intramuscular and subcutaneous immunizations of HTN-VLPs were sufficiently stimulated specific antibody response against Hantaan virus N protein and glycoproteins, which was comparable to Chinese commercial inactivated bivalent Hantaviruses Vaccine. Moreover, vaccination with HTN-VLPs also resulted in the induction of higher levels of specific cellular response to N protein than that of inactivated Vaccine. Our results provide an important insight towards the development of Hantaviruses-like particles as a potential candidate Vaccine for the control and prevention of Hantaviruses infection.
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Hantavirus-like particles generated in CHO cells induce specific immune responses in C57BL/6 mice
Vaccine, 2010Co-Authors: Feng Liu, Mifang Liang, Quanfu Zhang, Xiaofang Wang, Tao WangAbstract:A safe and effective Hantavirus Vaccine is highly desirable since Hantaviruses are distributed worldwide and cause an acute and often fatal disease (hemorrhagic fever with renal syndrome, HFRS). Virus-like particles (VLPs) displaying functional viral proteins could provide effective Vaccines against a few viruses, but their ability to induce Hantavirus-specific immune response has not been adequately investigated. To measure the immunogenicity of Hantaan virus-like particles (HTN-VLPs) Vaccine, we generated recombinant HTN-VLPs by co-expressing Hantaan virus nucleocapsid (N) protein and glycoproteins (Gn and Gc) in Chinese hamster ovary (CHO) cells. We compared intramuscular versus subcutaneous administration of HTN-VLPs for the ability to induce specific immune response against Hantaan virus infection. Mice that received both intramuscular and subcutaneous immunizations of HTN-VLPs were sufficiently stimulated specific antibody response against Hantaan virus N protein and glycoproteins, which was comparable to Chinese commercial inactivated bivalent Hantaviruses Vaccine. Moreover, vaccination with HTN-VLPs also resulted in the induction of higher levels of specific cellular response to N protein than that of inactivated Vaccine. Our results provide an important insight towards the development of Hantaviruses-like particles as a potential candidate Vaccine for the control and prevention of Hantaviruses infection.
