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Yukio Ishikawa - One of the best experts on this subject based on the ideXlab platform.
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chaperonin contributes to cold Hardiness of the onion maggot delia antiqua through repression of depolymerization of actin at low temperatures
PLOS ONE, 2009Co-Authors: Takumi Kayukawa, Yukio IshikawaAbstract:Winter-diapause and cold-acclimated non-diapause pupae of the onion maggot, Delia antiqua (Diptera: Anthomyiidae), show strong cold Hardiness. To obtain insights into the mechanisms involved in the enhancement of cold Hardiness, we investigated the expression patterns of genes encoding subunits of chaperonin (CCT) and the morphology of actin, a substrate of CCT, at low temperatures. Quantitative real-time PCR analyses showed the mRNA levels of CCT subunits in pupal tissues to be highly correlated with the cold Hardiness of the pupae. While actin in the Malpighian tubules of non-cold-hardy pupae showed extensive depolymerization after a cold treatment, actin in the same tissue of cold-hardy pupae was not depolymerized. Damage to cell membranes became apparent after the depolymerization of actin. Moreover, administration of Latrunculin B, an inhibitor of actin polymerization, to the larvae markedly decreased the cold Hardiness of the pupae obtained. These findings suggest that CCT contributes to the cold Hardiness of D. antiqua through the repression of depolymerization of actin at low temperatures.
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upregulation of a desaturase is associated with the enhancement of cold Hardiness in the onion maggot delia antiqua
Insect Biochemistry and Molecular Biology, 2007Co-Authors: Takumi Kayukawa, Bin Chen, Sugihiko Hoshizaki, Yukio IshikawaAbstract:Cold-acclimated non-diapause pupae, and summer- and winter-diapause pupae of the onion maggot, Delia antiqua (Diptera: Anthomyiidae), show marked cold Hardiness as compared with intact non-diapause pupae. Homeoviscous adaptation of cellular membranes is crucial to enhance the cold Hardiness of organisms, and Delta9-acyl-CoA desaturases have been assumed, albeit without experimental evidence in insects, to play a key role in the adaptation. We cloned the cDNA of a desaturase gene (Dadesat) from D. antiqua, which is most likely to encode Delta9-acyl-CoA desaturase. Expression of Dadesat mRNA in the brain, midgut, and Malpighian tubules of cold-acclimated and diapause pupae was upregulated 2-10 fold, correlating well with the increase in cold Hardiness. In the pupae with enhanced cold Hardiness, palmitoleic and oleic acids, the presumed products of Dadesat, in the phospholipids were significantly increased. These findings suggest that the increase in the expression of Dadesat contributes to enhanced cold Hardiness in D. antiqua through the production of these unsaturated fatty acids.
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expression of mrna for the t complex polypeptide 1 a subunit of chaperonin cct is upregulated in association with increased cold Hardiness in delia antiqua
Cell Stress & Chaperones, 2005Co-Authors: Takumi Kayukawa, Bin Chen, Shoichiro Miyazaki, Kyo Itoyama, Tetsuro Shinoda, Yukio IshikawaAbstract:Summer-diapause and winter-diapause pupae of the onion maggot, Delia antiqua (Diptera: Anthomyiidae), were significantly more cold hardy than nondiapause, prediapause, and postdiapause pupae. Moreover, cold acclimation of nondiapause pupae conferred strong cold Hardiness comparable with that of diapause pupae. Differential display analysis revealed that the expression of a gene encoding TCP-1 (the t-complex polypeptide–1), a subunit of chaperonin CCT, in D antiqua (DaTCP-1) is upregulated in the pupae that express enhanced cold Hardiness. Quantitative real-time polymerase chain reaction analyses showed that the levels of DaTCP-1 messenger RNA in pupal tissues, brain, and midgut in particular, are highly correlated with the cold Hardiness of the pupae. These findings suggest that the upregulation of DaTCP-1 expression is related to enhanced cold Hardiness in D antiqua. The upregulation of CCT in response to low temperature in an organism other than the yeast is newly reported.
Takumi Kayukawa - One of the best experts on this subject based on the ideXlab platform.
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chaperonin contributes to cold Hardiness of the onion maggot delia antiqua through repression of depolymerization of actin at low temperatures
PLOS ONE, 2009Co-Authors: Takumi Kayukawa, Yukio IshikawaAbstract:Winter-diapause and cold-acclimated non-diapause pupae of the onion maggot, Delia antiqua (Diptera: Anthomyiidae), show strong cold Hardiness. To obtain insights into the mechanisms involved in the enhancement of cold Hardiness, we investigated the expression patterns of genes encoding subunits of chaperonin (CCT) and the morphology of actin, a substrate of CCT, at low temperatures. Quantitative real-time PCR analyses showed the mRNA levels of CCT subunits in pupal tissues to be highly correlated with the cold Hardiness of the pupae. While actin in the Malpighian tubules of non-cold-hardy pupae showed extensive depolymerization after a cold treatment, actin in the same tissue of cold-hardy pupae was not depolymerized. Damage to cell membranes became apparent after the depolymerization of actin. Moreover, administration of Latrunculin B, an inhibitor of actin polymerization, to the larvae markedly decreased the cold Hardiness of the pupae obtained. These findings suggest that CCT contributes to the cold Hardiness of D. antiqua through the repression of depolymerization of actin at low temperatures.
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upregulation of a desaturase is associated with the enhancement of cold Hardiness in the onion maggot delia antiqua
Insect Biochemistry and Molecular Biology, 2007Co-Authors: Takumi Kayukawa, Bin Chen, Sugihiko Hoshizaki, Yukio IshikawaAbstract:Cold-acclimated non-diapause pupae, and summer- and winter-diapause pupae of the onion maggot, Delia antiqua (Diptera: Anthomyiidae), show marked cold Hardiness as compared with intact non-diapause pupae. Homeoviscous adaptation of cellular membranes is crucial to enhance the cold Hardiness of organisms, and Delta9-acyl-CoA desaturases have been assumed, albeit without experimental evidence in insects, to play a key role in the adaptation. We cloned the cDNA of a desaturase gene (Dadesat) from D. antiqua, which is most likely to encode Delta9-acyl-CoA desaturase. Expression of Dadesat mRNA in the brain, midgut, and Malpighian tubules of cold-acclimated and diapause pupae was upregulated 2-10 fold, correlating well with the increase in cold Hardiness. In the pupae with enhanced cold Hardiness, palmitoleic and oleic acids, the presumed products of Dadesat, in the phospholipids were significantly increased. These findings suggest that the increase in the expression of Dadesat contributes to enhanced cold Hardiness in D. antiqua through the production of these unsaturated fatty acids.
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expression of mrna for the t complex polypeptide 1 a subunit of chaperonin cct is upregulated in association with increased cold Hardiness in delia antiqua
Cell Stress & Chaperones, 2005Co-Authors: Takumi Kayukawa, Bin Chen, Shoichiro Miyazaki, Kyo Itoyama, Tetsuro Shinoda, Yukio IshikawaAbstract:Summer-diapause and winter-diapause pupae of the onion maggot, Delia antiqua (Diptera: Anthomyiidae), were significantly more cold hardy than nondiapause, prediapause, and postdiapause pupae. Moreover, cold acclimation of nondiapause pupae conferred strong cold Hardiness comparable with that of diapause pupae. Differential display analysis revealed that the expression of a gene encoding TCP-1 (the t-complex polypeptide–1), a subunit of chaperonin CCT, in D antiqua (DaTCP-1) is upregulated in the pupae that express enhanced cold Hardiness. Quantitative real-time polymerase chain reaction analyses showed that the levels of DaTCP-1 messenger RNA in pupal tissues, brain, and midgut in particular, are highly correlated with the cold Hardiness of the pupae. These findings suggest that the upregulation of DaTCP-1 expression is related to enhanced cold Hardiness in D antiqua. The upregulation of CCT in response to low temperature in an organism other than the yeast is newly reported.
Bin Chen - One of the best experts on this subject based on the ideXlab platform.
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upregulation of a desaturase is associated with the enhancement of cold Hardiness in the onion maggot delia antiqua
Insect Biochemistry and Molecular Biology, 2007Co-Authors: Takumi Kayukawa, Bin Chen, Sugihiko Hoshizaki, Yukio IshikawaAbstract:Cold-acclimated non-diapause pupae, and summer- and winter-diapause pupae of the onion maggot, Delia antiqua (Diptera: Anthomyiidae), show marked cold Hardiness as compared with intact non-diapause pupae. Homeoviscous adaptation of cellular membranes is crucial to enhance the cold Hardiness of organisms, and Delta9-acyl-CoA desaturases have been assumed, albeit without experimental evidence in insects, to play a key role in the adaptation. We cloned the cDNA of a desaturase gene (Dadesat) from D. antiqua, which is most likely to encode Delta9-acyl-CoA desaturase. Expression of Dadesat mRNA in the brain, midgut, and Malpighian tubules of cold-acclimated and diapause pupae was upregulated 2-10 fold, correlating well with the increase in cold Hardiness. In the pupae with enhanced cold Hardiness, palmitoleic and oleic acids, the presumed products of Dadesat, in the phospholipids were significantly increased. These findings suggest that the increase in the expression of Dadesat contributes to enhanced cold Hardiness in D. antiqua through the production of these unsaturated fatty acids.
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expression of mrna for the t complex polypeptide 1 a subunit of chaperonin cct is upregulated in association with increased cold Hardiness in delia antiqua
Cell Stress & Chaperones, 2005Co-Authors: Takumi Kayukawa, Bin Chen, Shoichiro Miyazaki, Kyo Itoyama, Tetsuro Shinoda, Yukio IshikawaAbstract:Summer-diapause and winter-diapause pupae of the onion maggot, Delia antiqua (Diptera: Anthomyiidae), were significantly more cold hardy than nondiapause, prediapause, and postdiapause pupae. Moreover, cold acclimation of nondiapause pupae conferred strong cold Hardiness comparable with that of diapause pupae. Differential display analysis revealed that the expression of a gene encoding TCP-1 (the t-complex polypeptide–1), a subunit of chaperonin CCT, in D antiqua (DaTCP-1) is upregulated in the pupae that express enhanced cold Hardiness. Quantitative real-time polymerase chain reaction analyses showed that the levels of DaTCP-1 messenger RNA in pupal tissues, brain, and midgut in particular, are highly correlated with the cold Hardiness of the pupae. These findings suggest that the upregulation of DaTCP-1 expression is related to enhanced cold Hardiness in D antiqua. The upregulation of CCT in response to low temperature in an organism other than the yeast is newly reported.
Keiichiro Matsukura - One of the best experts on this subject based on the ideXlab platform.
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changes in chemical components in the freshwater apple snail pomacea canaliculata gastropoda ampullariidae in relation to the development of its cold Hardiness
Cryobiology, 2008Co-Authors: Keiichiro Matsukura, Hisaaki Tsumuki, Yohei Izumi, Takashi WadaAbstract:The apple snail, Pomacea canaliculata, is an invasive freshwater snail. It increases its cold Hardiness before winter. However, the physiological mechanism of cold Hardiness in molluscs is poorly understood, especially in freshwater molluscs. In this study, we examined the changes in low molecular weight compounds, glycogen and lipids, in the body of P. canaliculata in association with the development of cold Hardiness. When snails without cold Hardiness were experimentally cold-acclimated, the amount of glycerol, glutamine, and carnosine increased, while glycogen and phenylalanine decreased. Overwintering cold-tolerant snails collected from a drained paddy field in November also showed increased glycerol in their bodies with decreasing glycogen concentration, compared to summer snails collected from a submerged field. Water content also decreased during the cold acclimation, although the water loss was minimal. These results indicate that the freshwater snail, P. canaliculata enhances cold Hardiness by accumulation of some kinds of low molecular weight compounds in its body as some insects do. However, the actual function of each low molecular compound is still unknown.
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seasonal changes in cold Hardiness of the invasive freshwater apple snail pomacea canaliculata lamarck gastropoda ampullariidae
Malacologia, 2007Co-Authors: Takashi Wada, Keiichiro MatsukuraAbstract:We investigated the cold Hardiness of a South American freshwater apple snail, Pomacea canaliculata, which began to invade Japanese paddy fields in the early 1980s. Pomacea canaliculata exhibited apparent seasonal fluctuation in its cold Hardiness. Snails collected from submerged paddy fields in summer were less tolerant to cold, and none survived exposure to 0°C for five days. With decreasing temperature, together with drainage of its habitat in autumn, P. canaliculata developed cold Hardiness, attaining almost 100% survivorship after exposure to 0°C for five days in December. The snails resting in drained fields were approximately nine times more cold tolerant than those collected from submerged fields, based on the time to 50% mortality at 0°C. Snails overwintering in aquatic conditions also acquired cold Hardiness.
Takashi Wada - One of the best experts on this subject based on the ideXlab platform.
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changes in chemical components in the freshwater apple snail pomacea canaliculata gastropoda ampullariidae in relation to the development of its cold Hardiness
Cryobiology, 2008Co-Authors: Keiichiro Matsukura, Hisaaki Tsumuki, Yohei Izumi, Takashi WadaAbstract:The apple snail, Pomacea canaliculata, is an invasive freshwater snail. It increases its cold Hardiness before winter. However, the physiological mechanism of cold Hardiness in molluscs is poorly understood, especially in freshwater molluscs. In this study, we examined the changes in low molecular weight compounds, glycogen and lipids, in the body of P. canaliculata in association with the development of cold Hardiness. When snails without cold Hardiness were experimentally cold-acclimated, the amount of glycerol, glutamine, and carnosine increased, while glycogen and phenylalanine decreased. Overwintering cold-tolerant snails collected from a drained paddy field in November also showed increased glycerol in their bodies with decreasing glycogen concentration, compared to summer snails collected from a submerged field. Water content also decreased during the cold acclimation, although the water loss was minimal. These results indicate that the freshwater snail, P. canaliculata enhances cold Hardiness by accumulation of some kinds of low molecular weight compounds in its body as some insects do. However, the actual function of each low molecular compound is still unknown.
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seasonal changes in cold Hardiness of the invasive freshwater apple snail pomacea canaliculata lamarck gastropoda ampullariidae
Malacologia, 2007Co-Authors: Takashi Wada, Keiichiro MatsukuraAbstract:We investigated the cold Hardiness of a South American freshwater apple snail, Pomacea canaliculata, which began to invade Japanese paddy fields in the early 1980s. Pomacea canaliculata exhibited apparent seasonal fluctuation in its cold Hardiness. Snails collected from submerged paddy fields in summer were less tolerant to cold, and none survived exposure to 0°C for five days. With decreasing temperature, together with drainage of its habitat in autumn, P. canaliculata developed cold Hardiness, attaining almost 100% survivorship after exposure to 0°C for five days in December. The snails resting in drained fields were approximately nine times more cold tolerant than those collected from submerged fields, based on the time to 50% mortality at 0°C. Snails overwintering in aquatic conditions also acquired cold Hardiness.
