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Suzana Beatriz Verissimo De Mello - One of the best experts on this subject based on the ideXlab platform.
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effect of isolated fractions of harpagophytum procumbens d c devil s claw on cox 1 cox 2 activity and nitric oxide production on whole blood assay
Phytotherapy Research, 2010Co-Authors: Maria Cecilia Anauate, Luce Maria Brandao Torres, Suzana Beatriz Verissimo De MelloAbstract:The present study evaluates the effect of isolated fractions of Harpagophytum procumbens (devil's claw) on cyclooxygenase (COX-1 and COX-2) activities and NO production using a whole blood assay. The activity of COX-1 was quantified as platelet thromboxane B2 production in blood clotting and COX-2 as prostaglandin E2 production in LPS-stimulated whole blood. Total NO2−/NO3− concentration was determined by Griess reaction in LPS stimulated blood. Assays were performed by incubation of isolated fractions obtained by flash chromatography monitored with HPLC, TLC and identified by 1HNMR, containing different amounts of Harpagoside with blood from healthy donors. Indomethacin and etoricoxib were the positive controls of COX-1 and COX-2 Inhibition. Data shows that fraction containing the highest concentration of Harpagoside inhibited indistinctively COX-1 and COX-2 (37.2 and 29.5% respectively) activity and greatly inhibited NO production (66%). In contrast the fraction including iridoid pool increased COX-2 and did not alter NO and COX-1 activities. The fraction containing cinnamic acid was able to reduce only NO production (67%). Our results demonstrated that the Harpagoside fraction is the main responsible for the effect of devils claw on these enzyme activities. However, other components from devil's claw crude extract could antagonize or increase the synthesis of inflammatory mediators. Copyright © 2010 John Wiley & Sons, Ltd.
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Effect of Isolated Fractions of Harpagophytum procumbens DC (Devil`s Claw) on COX-1, COX-2 Activity and Nitric Oxide Production on Whole-Blood Assay
WILEY-BLACKWELL, 2010Co-Authors: Anauate, Maria Cecilia, Torres, Luce Maria, Suzana Beatriz Verissimo De MelloAbstract:The present study evaluates the effect of isolated fractions of Harpagophytum procumbens (devil`s claw) on cyclooxygenase (COX-1 and COX-2) activities and NO production using a whole blood assay. The activity of COX-1 was quantified as platelet thromboxane B(2) production in blood clotting and COX-2 as prostaglandin E(2) production in LPS-stimulated whole blood. Total NO(2)(-)/NO(3)(-) concentration was determined by Griess reaction in LPS stimulated blood. Assays were performed by incubation of isolated fractions obtained by flash chromatography monitored with HPLC, TLC and identified by (1)HNMR, containing different amounts of Harpagoside with blood from healthy donors. Indomethacin and etoricoxib were the positive controls of COX-1 and COX-2 Inhibition. Data shows that fraction containing the highest concentration of Harpagoside inhibited indistinctively COX-1 and COX-2 (37.2 and 29.5% respectively) activity and greatly inhibited NO production (66%). In contrast the fraction including iridoid pool increased COX-2 and did not alter NO and COX-1 activities. The fraction containing cinnamic acid was able to reduce only NO production (67%). Our results demonstrated that the Harpagoside fraction is the main responsible for the effect of devils claw on these enzyme activities. However, other components from devil`s claw crude extract could antagonize or increase the synthesis of inflammatory mediators. Copyright (C) 2010 John Wiley & Sons, Ltd.FAPESP[04/02452-7
Taomin Huang - One of the best experts on this subject based on the ideXlab platform.
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infrared assisted extraction followed by high performance liquid chromatography to determine angoroside c cinnamic acid and Harpagoside content in scrophularia ningpoensis
BMC Complementary and Alternative Medicine, 2019Co-Authors: Yinghui Deng, Nianzu Chen, Xiuwen Zhang, Taomin HuangAbstract:Angoroside C, cinnamic acid, and Harpagoside are bioactive constituents in Scrophularia ningpoensis. Currently, an infrared-assisted extraction (IRAE) method coupled with high-performance liquid chromatography with ultraviolet detection (HPLC-UV) for the analysis of bioactive constituents in this plant is lacking. A method based on HPLC following IRAE has been developed for quantifying angoroside C, cinnamic acid, and Harpagoside in Scrophularia ningpoensis. Four main factors, namely, extraction solvent, solid/liquid ratio, illumination time, and distance between the infrared lamp and the round-bottom flask, were optimized for extraction. Furthermore, conventional ultrasonic extraction (USE) and microwave-assisted extraction (MAE) were also investigated to validate the developed method. The optimal extraction conditions were as follows: ethanol concentration, 37.5%; solid/liquid ratio, 1:25; illumination time, 10 min; and distance between infrared lamp and round-bottom flask, 3 cm. The results of method validation demonstrated that the developed method meets the requirement of analysis. The results show that the IRAE-HPLC is a simple, accurate, and green analytical preparatory method for the potential extraction and quantification of angoroside C, cinnamic acid, and Harpagoside in Scrophularia ningpoensis.
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rapid determination of cinnamic acid and Harpagoside in a traditional chinese medicine of scrophularia ningpoensis by microwave assisted extraction followed by high performance liquid chromatography hplc
Journal of Medicinal Plants Research, 2011Co-Authors: Taomin Huang, Nianzu Chen, Yonghua Lai, Donglei Wang, Jinchao YanAbstract:Cinnamic acid and Harpagoside are important active components present in Scrophularia ningpoensis, which has been used in the treatment of several diseases such as pharyngalgia. In the present study, a novel method based on high performance liquid chromatography (HPLC) following microwave-assisted extraction (MAE) was developed for the determination of cinnamic acid and Harpagoside in a traditional Chinese medicine (TCM) of S. ningpoensis. Effective chromatographic separation was achieved on a C18 column at the detection wavelength of 278 nm. Microwave power of 400 W and irradiation time of 4 min were found to be the optimum conditions for the MAE process. In addition, to demonstrate the feasibility of the MAE-HPLC method, the conventional ultrasound extraction (USE) method was used for the analysis of cinnamic acid and Harpagoside in the TCM. The results indicated that MAE-HPLC is a simple, rapid, efficient, and low cost method for the determination of cinnamic acid and Harpagoside in TCM as well as a potential tool for the assessment of TCM quality. Key words: Scrophularia ningpoensis, Cinnamic acid, Harpagoside, microwave-assisted extraction, liquid chromatography, traditional Chinese medicine.
Sigrun Chrubasik - One of the best experts on this subject based on the ideXlab platform.
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Harpagoside suppresses lipopolysaccharide induced inos and cox 2 expression through inhibition of nf κb activation
Journal of Ethnopharmacology, 2006Co-Authors: Tom Hsunwei Huang, Sigrun Chrubasik, Van Hoan Tran, Rujee K Duke, Sharon Tan, Basil D Roufogalis, Colin C DukeAbstract:Preparations of Harpagophytum procumbens, known as devil's claw, are used as an adjunctive therapy for the treatment of pain and osteoarthritis. Pharmacological evaluations have proven the effectiveness of this herbal drug as an anti-inflammatory and analgesic agent. The present study has investigated the mechanism of action of Harpagoside, one of the major components of Harpagophytum procumbens, using human HepG2 hepatocarcinoma and RAW 264.7 macrophage cell lines. Harpagoside inhibited lipopolysaccharide-induced mRNA levels and protein expression of cyclooxygenase-2 and inducible nitric oxide in HepG2 cells. These inhibitions appeared to correlate with the suppression of NF-κB activation by Harpagoside, as pre-treating cells with Harpagoside blocked the translocation of NF-κB into the nuclear compartments and degradation of the inhibitory subunit IκB-α. Furthermore, Harpagoside dose-dependently inhibited LPS-stimulated NF-κB promoter activity in a gene reporter assay in RAW 264.7 cells, indicating that Harpagoside interfered with the activation of gene transcription. These results suggest that the inhibition of the expression of cyclooxygenase-2 and inducible nitric oxide by Harpagoside involves suppression of NF-κB activation, thereby inhibiting downstream inflammation and subsequent pain events.
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Physicochemical properties of Harpagoside and its in vitro release from Harpagophytum procumbens extract tablets.
Phytomedicine, 2000Co-Authors: Sigrun Chrubasik, Frank Sporer, R. Dillmann-marschner, A. Friedmann, Michael WinkAbstract:Summary The objective of this investigation was to characterize the active-component Harpagoside of Harpagophytum extract from a physico-chemical perspective and to determine its in-vitro release from tablets according to DAB 1996. It was found that both pure Harpagoside and Harpagoside in Harpagophytum extract have an octanol-water distribution coefficient of approximately 4 which is neither dependent on temperature nor on pH. The mean Harpagoside content in Harpagophytum tablets of Batch 9102 was 16.4 mg (S.D. 0.2; S.E. 0.03). Related to a tablet weight of 365 mg (100%), this corresponds to a haragoside content of 4.5% (S.D. 0.049; S.E. 0.006). On average the tablets disintegrate after 18 ± 3 minutes (mean ± SD). The tablets taken from Batch 9102 released the active component Harpagoside well, with a t50 of 13.5 min, a t90 of 23 min and a t95 of 25 min in relation to 16.5 mg of Harpagoside per dose. Harpagoside content decreased by about 10% in artificial gastric fluid within a period of 3 hours and remained stable in artificial intestinal fluid for a period of 6 hours.
P.c. Schmidt - One of the best experts on this subject based on the ideXlab platform.
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high anti inflammatory activity of Harpagoside enriched extracts obtained from solvent modified super and subcritical carbon dioxide extractions of the roots of harpagophytum procumbens
Phytochemical Analysis, 2006Co-Authors: Marcel Gunther, Stefan Laufer, P.c. SchmidtAbstract:Solvent-modified carbon dioxide extractions of the roots of Harpagophytum procumbens have been investigated with respect to extraction efficiency and content of Harpagoside, and compared with a conventional extract. The effects of pressure, temperature, type and concentration of the modifier have been examined. Two extraction steps were necessary in order to achievehigh anti-inflammatory Harpagoside-enriched extracts. The first extraction step was carried out in the supercritical state using carbon dioxide modified with n-propanol to remove undesired lipophilic substances. The main extraction was performed either in the supercritical or in the subcritical state with carbon dioxide modified with ethanol. The supercritical fluid extraction resulted in extracts containing up to 30% Harpagoside. The subcritical extracts showed a Harpagoside content of ca. 20%, but the extraction yield was nearly three times greater compared with supercritical conditions. The total Harpagoside recovery resulting from the sum of the extract and the crude drug residue was greater than 99% in all experiments. The conventional extract and two carbon dioxide extracts were tested for in-vitro inhibition of 5-lipoxygenase or cyclooxygenase-2 biosynthesis. Both carbon dioxide extracts showed total inhibition on 5-lipoxygenase biosynthesis at a concentration of 51.8 mg/L. In contrast, the conventional extract failed to show any inhibition of 5-lipoxygenase biosynthesis.
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Comparison between HPLC and HPTLC-densitometry for the determination of Harpagoside from Harpagophytum procumbens CO2-extracts
Journal of Pharmaceutical and Biomedical Analysis, 2005Co-Authors: M. Günther, P.c. SchmidtAbstract:Abstract Carbon dioxide (CO 2 ) extracts of the secondary roots of Harpagophytum procumbens were quantified by high performance liquid chromatography (HPLC) and high performance thin layer chromatography (HPTLC). An isocratic HPLC method was used for the quantification of the iridoid glucoside Harpagoside at 278 nm. A HPTLC assay was developed for the determination of Harpagoside after coloration at 509 nm. The diode array detection of both analytical assays were used to examine the purity of Harpagoside peaks and compared with the standards, respectively. The assays provide good accuracy, reproducibility and selectivity for the quantitative analysis of Harpagoside. The Harpagoside contents of 15 different CO 2 -extracts were compared by HPLC and HPTLC-densitometry. The quantitative results of both analytical methods did not show any statistical significance between each other, although a trend to slightly lower mean values could be found for the HPTLC method.
Evelyne Ollivier - One of the best experts on this subject based on the ideXlab platform.
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Formation of nitrogen-containing metabolites from the main iridoids of Harpagophytum procumbens and H. zeyheri by human intestinal bacteria.
Planta Medica, 1999Co-Authors: Béatrice Baghdikian, Evelyne Ollivier, H. Guiraud-dauriac, Annie N'guyen, Gérard Duménil, Guy BalansardAbstract:Abstract The study of the metabolism of iridoid glycosides from Harpagophytum procumbens and Harpagophytum zeyheri by human intestinal bacteria, was realized in order to elucidate compounds responsible for the pharmacological activities of Harpagophytum. Harpagide, Harpagoside and 8-O-p-coumaroyl-harpagide were transformed into the pyridine monoterpene alkaloid aucubinine B by human fecal flora and by bacteria isolated from this flora. Aucubinine B was also prepared from harpagide, Harpagoside and 8-O-p-coumaroylharpagide, by beta-glucosidase in the presence of NH4+.
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reverse phase high pressure liquid chromatographic analysis of Harpagoside scorodioside and verbascoside from scrophularia scorodonia quantitative determination of Harpagoside
Planta Medica, 1998Co-Authors: A M Diaz, Evelyne Ollivier, Lidia Fernandez, T Martin, Lucinda Villaescusa, G BalansardAbstract:A reversed-phase high performance liquid chromatographic method has been developed for the determination of the main compounds (Harpagoside, scorodioside, and verbascoside) from different samples of Scrophularia scorodonia. The chromatographic method has been validated and applied for quantitative determination of Harpagoside. The results show the highest levels of Harpagoside in the leaf extract. The purity and identity of peaks were controlled by diode-array detection and comparison with standards.
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An analytical study, anti-inflammatory and analgesic effects of Harpagophytum procumbens and Harpagophytum zeyheri.
Planta Medica, 1997Co-Authors: Béatrice Baghdikian, Marie-claire Lanhers, Jacques Fleurentin, Evelyne Ollivier, C. Maillard, Guy Balansard, François MortierAbstract:Abstract The iridoids of Harpagophytum procumbens and Harpagophytum zeyheri were studied by CLHP. Harpagoside is the main iridoid for both drugs whereas 8-p-coumaroylharpagide is a representative iridoid of Harpagophytum zeyheri only. The ratio Harpagoside/8-p-coumaroylharpagide can be used to distinguish chemically both species. For commercial dried aqueous extracts this ratio is intermediate because they are probably prepared from a mixture of H. procumbens and H. zeyheri drugs. The aqueous extracts of both drugs show similar analgesic and anti-inflammatory properties. Harpagophytum procumbens and Harpagophytum zeyheri should be accepted as sources for the drug Harpagophyti radix.
