The Experts below are selected from a list of 12051 Experts worldwide ranked by ideXlab platform
Andre Mickel - One of the best experts on this subject based on the ideXlab platform.
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comparison of il 1β tnf α HBD 2 and HBD 3 expression in the dental pulp of smokers versus nonsmokers
Journal of Endodontics, 2017Co-Authors: Caroline Ghattas Ayoub, Anita Aminoshariae, Mohammed Bakkar, Santosh K. Ghosh, Tracey L. Bonfield, Catherine A. Demko, Thomas A. Montagnese, Andre MickelAbstract:INTRODUCTION To date, the endodontic literature lacks research on the effect of smoking on cytokine and defensin expression in the dental pulp. Therefore, the aim of this study was to investigate the expression of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, human beta defensin (HBD)-2 and HBD-3 in the dental pulp of smokers and compare them with nonsmokers. We hypothesized that cytokine and defensin expression would be reduced in smokers as compared with nonsmokers. METHODS Thirty-two smokers and 37 nonsmokers with endodontic pulpal diagnoses of normal, symptomatic irreversible pulpitis and asymptomatic irreversible pulpitis were included in this cross-sectional study. Samples from pulp chambers were collected and stored in phosphate-buffered saline at -80°C. Luminex was used to measure IL-1β and TNF-α levels. The levels of HBD-2 and HBD-3 were measured using enzyme-linked immunosorbent assay. Marker levels were normalized to protein concentrations and data were analyzed using Kruskal-Wallis test, Mann-Whitney U test, and 2-way analysis of variance (α = 0.05). RESULTS Pulpal concentrations of TNF-α and HBD-2 were significantly lower among smokers (P < .01), whereas no significant difference was observed for IL-1β, or HBD-3. Two-way analysis of covariance revealed that smoking status (P < .001), not endodontic diagnosis (pulpal status), significantly affected TNF-α and HBD-2 levels. CONCLUSIONS This study reported that smokers are immunologically deficient in TNF-α and HBD-2, suggesting that dental pulps of smokers possess limited defense mechanisms, affecting their endodontic prognosis and indicating a cause for their reported inferior outcome.
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Comparison of IL-1β, TNF-α, HBD-2, and HBD-3 Expression in the Dental Pulp of Smokers Versus Nonsmokers.
Journal of endodontics, 2017Co-Authors: Caroline Ghattas Ayoub, Anita Aminoshariae, Mohammed Bakkar, Santosh K. Ghosh, Tracey L. Bonfield, Catherine A. Demko, Thomas A. Montagnese, Andre MickelAbstract:INTRODUCTION To date, the endodontic literature lacks research on the effect of smoking on cytokine and defensin expression in the dental pulp. Therefore, the aim of this study was to investigate the expression of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, human beta defensin (HBD)-2 and HBD-3 in the dental pulp of smokers and compare them with nonsmokers. We hypothesized that cytokine and defensin expression would be reduced in smokers as compared with nonsmokers. METHODS Thirty-two smokers and 37 nonsmokers with endodontic pulpal diagnoses of normal, symptomatic irreversible pulpitis and asymptomatic irreversible pulpitis were included in this cross-sectional study. Samples from pulp chambers were collected and stored in phosphate-buffered saline at -80°C. Luminex was used to measure IL-1β and TNF-α levels. The levels of HBD-2 and HBD-3 were measured using enzyme-linked immunosorbent assay. Marker levels were normalized to protein concentrations and data were analyzed using Kruskal-Wallis test, Mann-Whitney U test, and 2-way analysis of variance (α = 0.05). RESULTS Pulpal concentrations of TNF-α and HBD-2 were significantly lower among smokers (P
Veronica Zagaclavellina - One of the best experts on this subject based on the ideXlab platform.
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tissue specific human beta defensins HBD 1 HBD 2 and HBD 3 secretion profile from human amniochorionic membranes stimulated with candida albicans in a two compartment tissue culture system
Reproductive Biology and Endocrinology, 2012Co-Authors: Veronica Zagaclavellina, Pilar Floresespinosa, Martha Ruiz, Rodrigo Vegasanchez, Arturo Florespliego, Guadalupe Estradagutierrez, Irma Sosagonzalez, Iyari Moralesmendez, Mauricio OsoriocaballeroAbstract:Background During intrauterine infection, amniochorionic membranes represent a mechanical and immunological barrier against dissemination of infection. Human beta defensins (HBD)-1, HBD-2, and HBD-3 are key elements of innate immunity that represent the first line of defense against different pathogen microorganisms associated with preterm labor. The aim of this work was to characterize the individual contribution of the amnion (AMN) and choriodecidua (CHD) regions to the secretion of HBD-1, HBD-2 and HBD-3, after stimulation with Candida albicans.
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in vitro secretion profile of pro inflammatory cytokines il 1β tnf α il 6 and of human beta defensins HBD 1 HBD 2 and HBD 3 from human chorioamniotic membranes after selective stimulation with gardnerella vaginalis
American Journal of Reproductive Immunology, 2012Co-Authors: Veronica Zagaclavellina, Ruiz Velascomunoz Martha, Pilar FloresespinosaAbstract:Citation Zaga-Clavellina V, Martha RV-M, Flores-Espinosa P. In vitro secretion profile of pro-inflammatory cytokines IL-1β, TNF-α, IL-6, and of human beta-defensins (HBD)-1, HBD-2, and HBD-3 from human chorioamniotic membranes after selective stimulation with Gardnerella vaginalis. Am J Reprod Immunol 2012; 67: 34–43 Problem Preterm labor associated with infection is a major clinical condition; in this work, we analyze the response of human chorioamniotic membranes stimulated with Gardnerella vaginalis. Method of study Using a two-compartment experimental model, 1 × 106 CFU/mL of G. vaginalis were added to either the amnion or choriodecidua face or to both. Concentrations of IL-1β, TNF-α, and IL-6, as well as human beta defensins (HBD) 1-3 were quantified by ELISA. Results In comparison with control conditions and regardless of the stimulation modality, IL-1β and IL-6 increased 4-fold and 28-fold, respectively, in the choriodecidual compartment. HBD-1 increased 2-fold mainly in the amniotic compartment when the stimulus was applied directly to this region. HBD-2 and HBD-3 increased an average of 2- and 8-fold, respectively, in the choriodecidual region. Conclusions Stimulation with G. vaginalis induced a tissue-specific secretion profile of 1L-1β, IL-6, and HBD 1-3 in the chorioamniotic membranes.
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tissue specific human beta defensins HBD 1 HBD2 and HBD3 secretion from human extra placental membranes stimulated with escherichia coli
Reproductive Biology and Endocrinology, 2010Co-Authors: Guadalupe Garcialopez, Pilar Floresespinosa, Veronica ZagaclavellinaAbstract:Background During an ascending infection along the reproductive tract, the extra-placental membranes must act as a selective and competent barrier against pathogens. Human beta defensins (HBD)1, HBD2, and HBD3 are key elements of innate immunity that are secreted to neutralize/control the progression of infection.
Ko Okumura - One of the best experts on this subject based on the ideXlab platform.
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antimicrobial peptides human β defensin HBD 3 and HBD 4 activate mast cells and increase skin vascular permeability
European Journal of Immunology, 2007Co-Authors: Xuejun Chen, François Niyonsaba, Hiroko Ushio, Mutsuko Hara, Hidenori Yokoi, Kenji Matsumoto, Hirohisa Saito, Isao Nagaoka, Shigaku Ikeda, Ko OkumuraAbstract:Antimicrobial peptides human b-defensins (HBD) are mainly produced by epithelia of several organs including skin, and participate in innate immunity by killing invading pathogens. Besides their microbicidal activities, HBD activate several inflammatory and immune cells. Since HBD are generated by tissues where mast cells are present, we hypothesized that these peptides could activate mast cells. In this study, we demonstrated that both HBD-3 and HBD-4 induced mast cell degranulation, prostaglandin D2 production, intracellular Ca 2+ mobilization and chemotaxis. Furthermore, HBD-3- and HBD-4-induced activation of mast cells was suppressed by pertussis toxin and U-73122, inhibitors for G protein and phospholipase C, respectively. We further revealed that HBD-3 and HBD-4 increased vascular permeability in the skin, which was dependent on the presence of mast cells, because HBD-3 and HBD-4 failed to enhance vascular permeability in mast cell-deficient Ws/Ws rats. We also demonstrated that HBD-3 and HBD-4 induced phosphorylation of MAPK p38 and ERK1/2, which were further required for HBD-mediated mast cell activation, as evidenced by the inhibitory effects of p38 and ERK1/2 inhibitors on mast cell degranulation. Together, these findings suggest the key role of HBD in inflammatory responses by recruiting and activating mast cells, and increasing vascular permeability.
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Antimicrobial peptides human β‐defensin (HBD)‐3 and HBD‐4 activate mast cells and increase skin vascular permeability
European journal of immunology, 2007Co-Authors: Xuejun Chen, François Niyonsaba, Hiroko Ushio, Mutsuko Hara, Hidenori Yokoi, Kenji Matsumoto, Hirohisa Saito, Isao Nagaoka, Shigaku Ikeda, Ko OkumuraAbstract:Antimicrobial peptides human b-defensins (HBD) are mainly produced by epithelia of several organs including skin, and participate in innate immunity by killing invading pathogens. Besides their microbicidal activities, HBD activate several inflammatory and immune cells. Since HBD are generated by tissues where mast cells are present, we hypothesized that these peptides could activate mast cells. In this study, we demonstrated that both HBD-3 and HBD-4 induced mast cell degranulation, prostaglandin D2 production, intracellular Ca 2+ mobilization and chemotaxis. Furthermore, HBD-3- and HBD-4-induced activation of mast cells was suppressed by pertussis toxin and U-73122, inhibitors for G protein and phospholipase C, respectively. We further revealed that HBD-3 and HBD-4 increased vascular permeability in the skin, which was dependent on the presence of mast cells, because HBD-3 and HBD-4 failed to enhance vascular permeability in mast cell-deficient Ws/Ws rats. We also demonstrated that HBD-3 and HBD-4 induced phosphorylation of MAPK p38 and ERK1/2, which were further required for HBD-mediated mast cell activation, as evidenced by the inhibitory effects of p38 and ERK1/2 inhibitors on mast cell degranulation. Together, these findings suggest the key role of HBD in inflammatory responses by recruiting and activating mast cells, and increasing vascular permeability.
Caroline Ghattas Ayoub - One of the best experts on this subject based on the ideXlab platform.
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comparison of il 1β tnf α HBD 2 and HBD 3 expression in the dental pulp of smokers versus nonsmokers
Journal of Endodontics, 2017Co-Authors: Caroline Ghattas Ayoub, Anita Aminoshariae, Mohammed Bakkar, Santosh K. Ghosh, Tracey L. Bonfield, Catherine A. Demko, Thomas A. Montagnese, Andre MickelAbstract:INTRODUCTION To date, the endodontic literature lacks research on the effect of smoking on cytokine and defensin expression in the dental pulp. Therefore, the aim of this study was to investigate the expression of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, human beta defensin (HBD)-2 and HBD-3 in the dental pulp of smokers and compare them with nonsmokers. We hypothesized that cytokine and defensin expression would be reduced in smokers as compared with nonsmokers. METHODS Thirty-two smokers and 37 nonsmokers with endodontic pulpal diagnoses of normal, symptomatic irreversible pulpitis and asymptomatic irreversible pulpitis were included in this cross-sectional study. Samples from pulp chambers were collected and stored in phosphate-buffered saline at -80°C. Luminex was used to measure IL-1β and TNF-α levels. The levels of HBD-2 and HBD-3 were measured using enzyme-linked immunosorbent assay. Marker levels were normalized to protein concentrations and data were analyzed using Kruskal-Wallis test, Mann-Whitney U test, and 2-way analysis of variance (α = 0.05). RESULTS Pulpal concentrations of TNF-α and HBD-2 were significantly lower among smokers (P < .01), whereas no significant difference was observed for IL-1β, or HBD-3. Two-way analysis of covariance revealed that smoking status (P < .001), not endodontic diagnosis (pulpal status), significantly affected TNF-α and HBD-2 levels. CONCLUSIONS This study reported that smokers are immunologically deficient in TNF-α and HBD-2, suggesting that dental pulps of smokers possess limited defense mechanisms, affecting their endodontic prognosis and indicating a cause for their reported inferior outcome.
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Comparison of IL-1β, TNF-α, HBD-2, and HBD-3 Expression in the Dental Pulp of Smokers Versus Nonsmokers.
Journal of endodontics, 2017Co-Authors: Caroline Ghattas Ayoub, Anita Aminoshariae, Mohammed Bakkar, Santosh K. Ghosh, Tracey L. Bonfield, Catherine A. Demko, Thomas A. Montagnese, Andre MickelAbstract:INTRODUCTION To date, the endodontic literature lacks research on the effect of smoking on cytokine and defensin expression in the dental pulp. Therefore, the aim of this study was to investigate the expression of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, human beta defensin (HBD)-2 and HBD-3 in the dental pulp of smokers and compare them with nonsmokers. We hypothesized that cytokine and defensin expression would be reduced in smokers as compared with nonsmokers. METHODS Thirty-two smokers and 37 nonsmokers with endodontic pulpal diagnoses of normal, symptomatic irreversible pulpitis and asymptomatic irreversible pulpitis were included in this cross-sectional study. Samples from pulp chambers were collected and stored in phosphate-buffered saline at -80°C. Luminex was used to measure IL-1β and TNF-α levels. The levels of HBD-2 and HBD-3 were measured using enzyme-linked immunosorbent assay. Marker levels were normalized to protein concentrations and data were analyzed using Kruskal-Wallis test, Mann-Whitney U test, and 2-way analysis of variance (α = 0.05). RESULTS Pulpal concentrations of TNF-α and HBD-2 were significantly lower among smokers (P
Pilar Floresespinosa - One of the best experts on this subject based on the ideXlab platform.
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tissue specific human beta defensins HBD 1 HBD 2 and HBD 3 secretion profile from human amniochorionic membranes stimulated with candida albicans in a two compartment tissue culture system
Reproductive Biology and Endocrinology, 2012Co-Authors: Veronica Zagaclavellina, Pilar Floresespinosa, Martha Ruiz, Rodrigo Vegasanchez, Arturo Florespliego, Guadalupe Estradagutierrez, Irma Sosagonzalez, Iyari Moralesmendez, Mauricio OsoriocaballeroAbstract:Background During intrauterine infection, amniochorionic membranes represent a mechanical and immunological barrier against dissemination of infection. Human beta defensins (HBD)-1, HBD-2, and HBD-3 are key elements of innate immunity that represent the first line of defense against different pathogen microorganisms associated with preterm labor. The aim of this work was to characterize the individual contribution of the amnion (AMN) and choriodecidua (CHD) regions to the secretion of HBD-1, HBD-2 and HBD-3, after stimulation with Candida albicans.
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in vitro secretion profile of pro inflammatory cytokines il 1β tnf α il 6 and of human beta defensins HBD 1 HBD 2 and HBD 3 from human chorioamniotic membranes after selective stimulation with gardnerella vaginalis
American Journal of Reproductive Immunology, 2012Co-Authors: Veronica Zagaclavellina, Ruiz Velascomunoz Martha, Pilar FloresespinosaAbstract:Citation Zaga-Clavellina V, Martha RV-M, Flores-Espinosa P. In vitro secretion profile of pro-inflammatory cytokines IL-1β, TNF-α, IL-6, and of human beta-defensins (HBD)-1, HBD-2, and HBD-3 from human chorioamniotic membranes after selective stimulation with Gardnerella vaginalis. Am J Reprod Immunol 2012; 67: 34–43 Problem Preterm labor associated with infection is a major clinical condition; in this work, we analyze the response of human chorioamniotic membranes stimulated with Gardnerella vaginalis. Method of study Using a two-compartment experimental model, 1 × 106 CFU/mL of G. vaginalis were added to either the amnion or choriodecidua face or to both. Concentrations of IL-1β, TNF-α, and IL-6, as well as human beta defensins (HBD) 1-3 were quantified by ELISA. Results In comparison with control conditions and regardless of the stimulation modality, IL-1β and IL-6 increased 4-fold and 28-fold, respectively, in the choriodecidual compartment. HBD-1 increased 2-fold mainly in the amniotic compartment when the stimulus was applied directly to this region. HBD-2 and HBD-3 increased an average of 2- and 8-fold, respectively, in the choriodecidual region. Conclusions Stimulation with G. vaginalis induced a tissue-specific secretion profile of 1L-1β, IL-6, and HBD 1-3 in the chorioamniotic membranes.
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tissue specific human beta defensins HBD 1 HBD2 and HBD3 secretion from human extra placental membranes stimulated with escherichia coli
Reproductive Biology and Endocrinology, 2010Co-Authors: Guadalupe Garcialopez, Pilar Floresespinosa, Veronica ZagaclavellinaAbstract:Background During an ascending infection along the reproductive tract, the extra-placental membranes must act as a selective and competent barrier against pathogens. Human beta defensins (HBD)1, HBD2, and HBD3 are key elements of innate immunity that are secreted to neutralize/control the progression of infection.
