The Experts below are selected from a list of 24 Experts worldwide ranked by ideXlab platform
Hans Peter Luther - One of the best experts on this subject based on the ideXlab platform.
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Expression of α1-adrenergic receptor subtypes in Heart Cell Culture
Molecular and Cellular Biochemistry, 2001Co-Authors: Svenia Podlowski, Wolfgang Schulze, Rosemarie Morwinski, Igor Buchwalow, Gert Baumann, Hans Peter LutherAbstract:Three α_1AR subtypes have been cloned so far and are designated as α_1a, α_1b, and α_1d. Organspecific distribution pattern and subtype-specific effects are known but not fully understood. To address a Cell-type specific expression pattern in the Heart we investigated expression pattern of α_1AR subtypes on RNA and proteinlevel in Heart tissue, Cultured cardiomyocytes and nonmyocytes of the rat. Each α_1ARsubtype mRNA was present in neonatal and adult rat Heart Culture but the relative distribution pattern was significantly different. While the α_1aAR subtype is preferentially expressed in adult cardiomyocytes, the α_1bAR subtype was preferentially expressed in the nonmyocyte Cell fraction. The RTPCR results were confirmed by Westernblotting (α_1b) and immunocytochemical studies. Incubation with an α_1agonist (phenylephrine) for 72 h led to a significant reduction of the α_1bAR in neonatal Heart Cell Culture on both mRNA and protein level. In contrast, incubation with an α_1antagonist (prazosin) induced a 1.6 fold upregulation of the α_1aAR mRNA without significant effects on radioligand binding and functional assay. The results indicate a distribution pattern of the α_1AR subtype which is specific for Cell type and ontogeny of the rat Heart and may be regulated by adrenergic agents.
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Expression of alpha1-adrenergic receptor subtypes in Heart Cell Culture.
Molecular and cellular biochemistry, 2001Co-Authors: Hans Peter Luther, Svenia Podlowski, Wolfgang Schulze, Rosemarie Morwinski, Igor Buchwalow, Gert Baumann, G WallukatAbstract:Three alpha1-AR subtypes have been cloned so far and are designated as alpha1a, alpha1b,, and alpha1d. Organ-specific distribution pattern and subtype-specific effects are known but not fully understood. To address a Cell-type specific expression pattern in the Heart we investigated expression pattern of alpha1-AR subtypes on RNA- and protein-level in Heart tissue, Cultured cardiomyocytes and non-myocytes of the rat. Each alpha1AR-subtype mRNA was present in neonatal and adult rat Heart Culture but the relative distribution pattern was significantly different. While the alpha1a-AR subtype is preferentially expressed in adult cardiomyocytes, the alpha1b-AR subtype was preferentially expressed in the non-myocyte Cell fraction. The RT-PCR results were confirmed by Western-blotting (alpha1b) and immunocytochemical studies. Incubation with an alpha1-agonist (phenylephrine) for 72 h led to a significant reduction of the alpha1b-AR in neonatal Heart Cell Culture on both mRNA and protein level. In contrast, incubation with an alpha1-antagonist (prazosin) induced a 1.6 fold upregulation of the alpha1a-AR mRNA without significant effects on radioligand binding and functional assay. The results indicate a distribution pattern of the alpha1-AR subtype which is specific for Cell type and ontogeny of the rat Heart and may be regulated by adrenergic agents.
Jonas B Galper - One of the best experts on this subject based on the ideXlab platform.
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co Culture of embryonic chick Heart Cells and ciliary ganglia induces parasympathetic responsiveness in embryonic chick Heart Cells
Biochemical Journal, 1993Co-Authors: Joey V Barnett, M Taniuchi, M B Yang, Jonas B GalperAbstract:We have developed a system for the co-Culture of embryonic chick Heart Cells obtained from embryos at 3.5 days in ovo with ciliary ganglia from chick embryos at 7 days in vivo. After 3 days of co-Culture, removal of the ciliary ganglia resulted in complete degeneration of axons within 6-8 h, leaving the post-innervated Heart Cell Culture devoid of neurons. Embryonic chick Heart Cells at 3.5 days in ovo are unresponsive to muscarinic stimulation. However, following 3 days of co-Culture with ciliary ganglia, the Heart Cells developed a negative chronotropic response to muscarinic stimulation (paired t test, P < 0.02) which persisted for at least 24 h after removal of the ciliary ganglion. The development of muscarinic responsiveness was associated with an increase in the levels of specific alpha-subunits of the guanine nucleotide binding proteins (G-proteins), with a 3-fold increase in the level of alpha 39 (39 kDa subunit) and a 2.5-fold increase in the level of alpha 41. The level of the G-protein subunit alpha s remained unchanged. Culture of embryonic chick Heart Cells at 3.5 days in ovo with medium conditioned by the growth of embryonic chick Heart Cells and ciliary ganglia had an effect on the chronotropic response to muscarinic stimulation and on alpha 39 and alpha 41 levels identical to that of co-Culture. These data suggest that a soluble factor released during the co-Culture of embryonic chick Heart Cells and ciliary ganglia is capable of inducing muscarinic responsiveness. These studies suggest that innervation of the Heart may induce parasympathetic responsiveness by increasing the availability of G-proteins which couple the muscarinic receptor to a physiological response.
Svenia Podlowski - One of the best experts on this subject based on the ideXlab platform.
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Expression of α1-adrenergic receptor subtypes in Heart Cell Culture
Molecular and Cellular Biochemistry, 2001Co-Authors: Svenia Podlowski, Wolfgang Schulze, Rosemarie Morwinski, Igor Buchwalow, Gert Baumann, Hans Peter LutherAbstract:Three α_1AR subtypes have been cloned so far and are designated as α_1a, α_1b, and α_1d. Organspecific distribution pattern and subtype-specific effects are known but not fully understood. To address a Cell-type specific expression pattern in the Heart we investigated expression pattern of α_1AR subtypes on RNA and proteinlevel in Heart tissue, Cultured cardiomyocytes and nonmyocytes of the rat. Each α_1ARsubtype mRNA was present in neonatal and adult rat Heart Culture but the relative distribution pattern was significantly different. While the α_1aAR subtype is preferentially expressed in adult cardiomyocytes, the α_1bAR subtype was preferentially expressed in the nonmyocyte Cell fraction. The RTPCR results were confirmed by Westernblotting (α_1b) and immunocytochemical studies. Incubation with an α_1agonist (phenylephrine) for 72 h led to a significant reduction of the α_1bAR in neonatal Heart Cell Culture on both mRNA and protein level. In contrast, incubation with an α_1antagonist (prazosin) induced a 1.6 fold upregulation of the α_1aAR mRNA without significant effects on radioligand binding and functional assay. The results indicate a distribution pattern of the α_1AR subtype which is specific for Cell type and ontogeny of the rat Heart and may be regulated by adrenergic agents.
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Expression of alpha1-adrenergic receptor subtypes in Heart Cell Culture.
Molecular and cellular biochemistry, 2001Co-Authors: Hans Peter Luther, Svenia Podlowski, Wolfgang Schulze, Rosemarie Morwinski, Igor Buchwalow, Gert Baumann, G WallukatAbstract:Three alpha1-AR subtypes have been cloned so far and are designated as alpha1a, alpha1b,, and alpha1d. Organ-specific distribution pattern and subtype-specific effects are known but not fully understood. To address a Cell-type specific expression pattern in the Heart we investigated expression pattern of alpha1-AR subtypes on RNA- and protein-level in Heart tissue, Cultured cardiomyocytes and non-myocytes of the rat. Each alpha1AR-subtype mRNA was present in neonatal and adult rat Heart Culture but the relative distribution pattern was significantly different. While the alpha1a-AR subtype is preferentially expressed in adult cardiomyocytes, the alpha1b-AR subtype was preferentially expressed in the non-myocyte Cell fraction. The RT-PCR results were confirmed by Western-blotting (alpha1b) and immunocytochemical studies. Incubation with an alpha1-agonist (phenylephrine) for 72 h led to a significant reduction of the alpha1b-AR in neonatal Heart Cell Culture on both mRNA and protein level. In contrast, incubation with an alpha1-antagonist (prazosin) induced a 1.6 fold upregulation of the alpha1a-AR mRNA without significant effects on radioligand binding and functional assay. The results indicate a distribution pattern of the alpha1-AR subtype which is specific for Cell type and ontogeny of the rat Heart and may be regulated by adrenergic agents.
Wolfgang Schulze - One of the best experts on this subject based on the ideXlab platform.
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Expression of α1-adrenergic receptor subtypes in Heart Cell Culture
Molecular and Cellular Biochemistry, 2001Co-Authors: Svenia Podlowski, Wolfgang Schulze, Rosemarie Morwinski, Igor Buchwalow, Gert Baumann, Hans Peter LutherAbstract:Three α_1AR subtypes have been cloned so far and are designated as α_1a, α_1b, and α_1d. Organspecific distribution pattern and subtype-specific effects are known but not fully understood. To address a Cell-type specific expression pattern in the Heart we investigated expression pattern of α_1AR subtypes on RNA and proteinlevel in Heart tissue, Cultured cardiomyocytes and nonmyocytes of the rat. Each α_1ARsubtype mRNA was present in neonatal and adult rat Heart Culture but the relative distribution pattern was significantly different. While the α_1aAR subtype is preferentially expressed in adult cardiomyocytes, the α_1bAR subtype was preferentially expressed in the nonmyocyte Cell fraction. The RTPCR results were confirmed by Westernblotting (α_1b) and immunocytochemical studies. Incubation with an α_1agonist (phenylephrine) for 72 h led to a significant reduction of the α_1bAR in neonatal Heart Cell Culture on both mRNA and protein level. In contrast, incubation with an α_1antagonist (prazosin) induced a 1.6 fold upregulation of the α_1aAR mRNA without significant effects on radioligand binding and functional assay. The results indicate a distribution pattern of the α_1AR subtype which is specific for Cell type and ontogeny of the rat Heart and may be regulated by adrenergic agents.
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Expression of alpha1-adrenergic receptor subtypes in Heart Cell Culture.
Molecular and cellular biochemistry, 2001Co-Authors: Hans Peter Luther, Svenia Podlowski, Wolfgang Schulze, Rosemarie Morwinski, Igor Buchwalow, Gert Baumann, G WallukatAbstract:Three alpha1-AR subtypes have been cloned so far and are designated as alpha1a, alpha1b,, and alpha1d. Organ-specific distribution pattern and subtype-specific effects are known but not fully understood. To address a Cell-type specific expression pattern in the Heart we investigated expression pattern of alpha1-AR subtypes on RNA- and protein-level in Heart tissue, Cultured cardiomyocytes and non-myocytes of the rat. Each alpha1AR-subtype mRNA was present in neonatal and adult rat Heart Culture but the relative distribution pattern was significantly different. While the alpha1a-AR subtype is preferentially expressed in adult cardiomyocytes, the alpha1b-AR subtype was preferentially expressed in the non-myocyte Cell fraction. The RT-PCR results were confirmed by Western-blotting (alpha1b) and immunocytochemical studies. Incubation with an alpha1-agonist (phenylephrine) for 72 h led to a significant reduction of the alpha1b-AR in neonatal Heart Cell Culture on both mRNA and protein level. In contrast, incubation with an alpha1-antagonist (prazosin) induced a 1.6 fold upregulation of the alpha1a-AR mRNA without significant effects on radioligand binding and functional assay. The results indicate a distribution pattern of the alpha1-AR subtype which is specific for Cell type and ontogeny of the rat Heart and may be regulated by adrenergic agents.
Rosemarie Morwinski - One of the best experts on this subject based on the ideXlab platform.
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Expression of α1-adrenergic receptor subtypes in Heart Cell Culture
Molecular and Cellular Biochemistry, 2001Co-Authors: Svenia Podlowski, Wolfgang Schulze, Rosemarie Morwinski, Igor Buchwalow, Gert Baumann, Hans Peter LutherAbstract:Three α_1AR subtypes have been cloned so far and are designated as α_1a, α_1b, and α_1d. Organspecific distribution pattern and subtype-specific effects are known but not fully understood. To address a Cell-type specific expression pattern in the Heart we investigated expression pattern of α_1AR subtypes on RNA and proteinlevel in Heart tissue, Cultured cardiomyocytes and nonmyocytes of the rat. Each α_1ARsubtype mRNA was present in neonatal and adult rat Heart Culture but the relative distribution pattern was significantly different. While the α_1aAR subtype is preferentially expressed in adult cardiomyocytes, the α_1bAR subtype was preferentially expressed in the nonmyocyte Cell fraction. The RTPCR results were confirmed by Westernblotting (α_1b) and immunocytochemical studies. Incubation with an α_1agonist (phenylephrine) for 72 h led to a significant reduction of the α_1bAR in neonatal Heart Cell Culture on both mRNA and protein level. In contrast, incubation with an α_1antagonist (prazosin) induced a 1.6 fold upregulation of the α_1aAR mRNA without significant effects on radioligand binding and functional assay. The results indicate a distribution pattern of the α_1AR subtype which is specific for Cell type and ontogeny of the rat Heart and may be regulated by adrenergic agents.
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Expression of alpha1-adrenergic receptor subtypes in Heart Cell Culture.
Molecular and cellular biochemistry, 2001Co-Authors: Hans Peter Luther, Svenia Podlowski, Wolfgang Schulze, Rosemarie Morwinski, Igor Buchwalow, Gert Baumann, G WallukatAbstract:Three alpha1-AR subtypes have been cloned so far and are designated as alpha1a, alpha1b,, and alpha1d. Organ-specific distribution pattern and subtype-specific effects are known but not fully understood. To address a Cell-type specific expression pattern in the Heart we investigated expression pattern of alpha1-AR subtypes on RNA- and protein-level in Heart tissue, Cultured cardiomyocytes and non-myocytes of the rat. Each alpha1AR-subtype mRNA was present in neonatal and adult rat Heart Culture but the relative distribution pattern was significantly different. While the alpha1a-AR subtype is preferentially expressed in adult cardiomyocytes, the alpha1b-AR subtype was preferentially expressed in the non-myocyte Cell fraction. The RT-PCR results were confirmed by Western-blotting (alpha1b) and immunocytochemical studies. Incubation with an alpha1-agonist (phenylephrine) for 72 h led to a significant reduction of the alpha1b-AR in neonatal Heart Cell Culture on both mRNA and protein level. In contrast, incubation with an alpha1-antagonist (prazosin) induced a 1.6 fold upregulation of the alpha1a-AR mRNA without significant effects on radioligand binding and functional assay. The results indicate a distribution pattern of the alpha1-AR subtype which is specific for Cell type and ontogeny of the rat Heart and may be regulated by adrenergic agents.
