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Bingru Huang - One of the best experts on this subject based on the ideXlab platform.
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abscisic acid mediation of drought priming enhanced Heat Tolerance in tall fescue festuca arundinacea and arabidopsis
Physiologia Plantarum, 2019Co-Authors: Xiaxiang Zhang, Xiuyun Wang, Lili Zhuang, Yanli Gao, Bingru HuangAbstract:Abscisic acid (ABA) may play roles in mediating cross stress Tolerance in plants. The objectives of this study were to investigate the priming effects of drought and ABA on Heat Tolerance and to determine how ABA may be involved in enhanced Heat Tolerance by drought. Focusing on the transcriptional level, two independent experiments were conducted, using a perennial grass species, tall fescue (Festuca arundinacea) and Arabidopsis. In experiment 1, tall fescue plants were exposed to mild drought by withholding irrigation for 8 days (drought priming) and foliar sprayed with ABA or an ABA-synthesis inhibitor (fluridone). After that they were subsequently subjected to Heat stress (38/33°C day/night) for 25 days in growth chambers. In experiment 2, Arabidopsis Columbia ecotype (wild-type) and ABA-deficient mutant (aba3-1, CS157) were pre-treated with drought priming and then exposed to Heat stress (45/40°C) for 3 days. The physiological analysis demonstrated that both drought priming and foliar application of ABA-enhanced Heat Tolerance in tall fescue, while drought priming had no significant effects on Heat Tolerance in ABA-deficient Arabidopsis plants. Application of fluridone to tall fescue and ABA-deficient mutants of Arabidopsis exhibited diminished or attenuated positive effects of drought priming on Heat Tolerance. ABA mediation of acquired Heat Tolerance by drought priming was associated with the upregulation of CDPK3, MPK3, DREB2A, AREB3, MYB2, MYC4, HsfA2, HSP18, and HSP70. Our study revealed the roles of ABA in drought priming-enhanced Heat Tolerance, which may involve transcriptional regulation for stress signaling, ABA responses and Heat protection.
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lipidomic reprogramming associated with drought stress priming enhanced Heat Tolerance in tall fescue festuca arundinacea
Plant Cell and Environment, 2019Co-Authors: Xiaxiang Zhang, Bingru HuangAbstract:Stress priming by exposing plants to a mild or moderate drought could enhance plant Tolerance to subsequent Heat stress. Lipids play vital roles in stress adaptation, but how lipidomic profiles change, affecting the cross-stress Tolerance, is largely unknown. The objectives of this study were to perform lipidomics, to analyse the content, composition, and saturation levels of lipids in leaves of tall fescue (Festuca arundinacea) following drought priming and subsequent Heat stress, and to identify major lipids and molecular species associated with priming-enhanced Heat Tolerance. Plants were initially exposed to drought for 8 days by withholding irrigation and subsequently subjected to 25 days of Heat stress (38/33°C day/night) in growth chambers. Drought-primed plants maintained significantly higher leaf relative water content, chlorophyll content, photochemical efficiency, and lower electrolyte leakage than nonprimed plants under Heat stress. Drought priming enhanced the accumulation of phospholipids and glycolipids involved in membrane stabilization and stress signalling (phosphatidic acid, phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol, and digalactosyl diacylglycerol) during subsequent exposure to Heat stress. The reprogramming of lipid metabolism for membrane stabilization and signalling in response to drought priming and subsequent exposure to Heat stress could contribute to drought priming-enhanced Heat Tolerance in cool-season grass species.
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characterization and functional analysis of fahsfc1b from festuca arundinacea conferring Heat Tolerance in arabidopsis
International Journal of Molecular Sciences, 2018Co-Authors: Lili Zhuang, Zhi Min Yang, Wei Cao, Jian Wang, Bingru HuangAbstract:Heat transcription factors (Hsfs) belong to a large gene family classified into A, B, and C groups, with classes A and B Hsfs being well-characterized and known for their roles in plant Tolerance to abiotic stresses. The functions and roles of Class C Hsfs are not well-documented. The objectives of this study were to characterize a class C Hsf gene (FaHsfC1b) cloned from tall fescue (Festuca arundinacea), a perennial grass species, and to determine the physiological functions of FaHsfC1b in regulating Heat Tolerance by overexpressing FaHsfC1b in Arabidopsis thaliana. Full length cDNA of FaHsfC1b was cloned and the sequence alignment showed that it had high similarity to OsHsfC1b with typical DNA binding domain, hydrophobic oligomerization domain, and a nucleus localization signal. Transient expression with FaHsfC1b-eGFP in protoplasts of Arabidopsis leaves indicated its nucleus localization. qRT-PCR analysis showed that FaHsfC1b responded to Heat, osmotic, salt, and cold stress in leaves and roots during 48-h treatment. Physiological analysis showed that FaHsfC1b overexpression enhanced plant survival rate, chlorophyll content, and photochemical efficiency, while it resulted in decreases in electrolyte leakage, H2O2 and O2− content under Heat stress. qRT-PCR showed that endogenous HsfC1 was induced in transgenic plants and the expression levels of Heat protection protein genes, including several HSPs, AtGalSyn1, AtRof1, and AtHSA32, as well as ABA-synthesizing gene (NCED3) were significantly upregulated in transgenic plants overexpressing FaHsfC1b under Heat stress. Our results first demonstrate that HsfC1b plays positive roles in plant Tolerance to Heat stress in association with the induction and upregulation of Heat-protective genes. HsfC1b may be used as a candidate gene for genetic modification of cool-season plant species for improving Heat Tolerance.
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butanediol enhanced Heat Tolerance in agrostis stolonifera in association with alteration in stress related gene expression and metabolic profiles
Environmental and Experimental Botany, 2018Co-Authors: Jing Zhang, Yi Shi, Bingru HuangAbstract:Abstract Butanediol (BD) is a bacterial volatile compound which can activate induced-systemic resistance to diseases in plants, but its effects on abiotic stress Tolerance are not well-known. The objectives of this study were to examine physiological effects of BD on Heat Tolerance in creeping bentgrass and to identify BD-responsive metabolites and genes contributing to effects of BD on Heat Tolerance. Creeping bentgrass plants (cv.’ PennA4’ and’ Penncross’) were treated with 2,3 -butanediol or water through foliar spray and were exposed to Heat stress (35/30 °C, day/night) or optimal temperature (20/15 °C) in growth chambers. Creeping bentgrass plants treated with BD exhibited improved Heat Tolerance, demonstrated by higher visual quality and leaf photochemical efficiency when compared with the untreated control plants. Real-time PCR revealed that BD application resulted in up-regulation of genes related to cell elongation, metabolism, and stress responses in plants exposed to Heat stress. Metabolite profiling identified a number of organic acids, sugars and sugar acids that accumulated due to BD treatment under Heat stress. Results of the current study suggest that BD is effective in improving Heat Tolerance in creeping bentgrass, mainly through the enhancement of gene expression and metabolite accumulation involved in energy metabolism and stress signaling.
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metabolic pathways involved in carbon dioxide enhanced Heat Tolerance in bermudagrass
Frontiers in Plant Science, 2017Co-Authors: Ningli Fan, Zhi Min Yang, Bingru HuangAbstract:Global climate changes involve elevated temperature and CO2 concentration, imposing significant impact on plant growth of various plant species. Elevated temperature exacerbates Heat damages, but elevated CO2 has positive effects on promoting plant growth and Heat Tolerance. The objective of this study was to identify metabolic pathways affected by elevated CO2 conferring the improvement of Heat Tolerance in a C4 perennial grass species, bermudagrass (Cynodon dactylon Pers.). Plants were planted under either ambient CO2 concentration (400 μmol⋅mol-1) or elevated CO2 concentration (800 μmol⋅mol-1) and subjected to ambient temperature (30/25°C, day/night) or Heat stress (45/40°C, day/night). Elevated CO2 concentration suppressed Heat-induced damages and improved Heat Tolerance in bermudagrass. The enhanced Heat Tolerance under elevated CO2 was attributed to some important metabolic pathways during which proteins and metabolites were up-regulated, including light reaction (ATP synthase subunit and photosystem I reaction center subunit) and carbon fixation [(glyceraldehyde-3-phosphate dehydrogenase, GAPDH), fructose-bisphosphate aldolase, phosphoglycerate kinase, sedoheptulose-1,7-bisphosphatase and sugars) of photosynthesis, glycolysis (GAPDH, glucose, fructose, and galactose) and TCA cycle (pyruvic acid, malic acid and malate dehydrogenase) of respiration, amino acid metabolism (aspartic acid, methionine, threonine, isoleucine, lysine, valine, alanine, and isoleucine) as well as the GABA shunt (GABA, glutamic acid, alanine, proline and 5-oxoproline). The up-regulation of those metabolic processes by elevated CO2 could at least partially contribute to the improvement of Heat Tolerance in perennial grass species.
Xiaxiang Zhang - One of the best experts on this subject based on the ideXlab platform.
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abscisic acid mediation of drought priming enhanced Heat Tolerance in tall fescue festuca arundinacea and arabidopsis
Physiologia Plantarum, 2019Co-Authors: Xiaxiang Zhang, Xiuyun Wang, Lili Zhuang, Yanli Gao, Bingru HuangAbstract:Abscisic acid (ABA) may play roles in mediating cross stress Tolerance in plants. The objectives of this study were to investigate the priming effects of drought and ABA on Heat Tolerance and to determine how ABA may be involved in enhanced Heat Tolerance by drought. Focusing on the transcriptional level, two independent experiments were conducted, using a perennial grass species, tall fescue (Festuca arundinacea) and Arabidopsis. In experiment 1, tall fescue plants were exposed to mild drought by withholding irrigation for 8 days (drought priming) and foliar sprayed with ABA or an ABA-synthesis inhibitor (fluridone). After that they were subsequently subjected to Heat stress (38/33°C day/night) for 25 days in growth chambers. In experiment 2, Arabidopsis Columbia ecotype (wild-type) and ABA-deficient mutant (aba3-1, CS157) were pre-treated with drought priming and then exposed to Heat stress (45/40°C) for 3 days. The physiological analysis demonstrated that both drought priming and foliar application of ABA-enhanced Heat Tolerance in tall fescue, while drought priming had no significant effects on Heat Tolerance in ABA-deficient Arabidopsis plants. Application of fluridone to tall fescue and ABA-deficient mutants of Arabidopsis exhibited diminished or attenuated positive effects of drought priming on Heat Tolerance. ABA mediation of acquired Heat Tolerance by drought priming was associated with the upregulation of CDPK3, MPK3, DREB2A, AREB3, MYB2, MYC4, HsfA2, HSP18, and HSP70. Our study revealed the roles of ABA in drought priming-enhanced Heat Tolerance, which may involve transcriptional regulation for stress signaling, ABA responses and Heat protection.
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lipidomic reprogramming associated with drought stress priming enhanced Heat Tolerance in tall fescue festuca arundinacea
Plant Cell and Environment, 2019Co-Authors: Xiaxiang Zhang, Bingru HuangAbstract:Stress priming by exposing plants to a mild or moderate drought could enhance plant Tolerance to subsequent Heat stress. Lipids play vital roles in stress adaptation, but how lipidomic profiles change, affecting the cross-stress Tolerance, is largely unknown. The objectives of this study were to perform lipidomics, to analyse the content, composition, and saturation levels of lipids in leaves of tall fescue (Festuca arundinacea) following drought priming and subsequent Heat stress, and to identify major lipids and molecular species associated with priming-enhanced Heat Tolerance. Plants were initially exposed to drought for 8 days by withholding irrigation and subsequently subjected to 25 days of Heat stress (38/33°C day/night) in growth chambers. Drought-primed plants maintained significantly higher leaf relative water content, chlorophyll content, photochemical efficiency, and lower electrolyte leakage than nonprimed plants under Heat stress. Drought priming enhanced the accumulation of phospholipids and glycolipids involved in membrane stabilization and stress signalling (phosphatidic acid, phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol, and digalactosyl diacylglycerol) during subsequent exposure to Heat stress. The reprogramming of lipid metabolism for membrane stabilization and signalling in response to drought priming and subsequent exposure to Heat stress could contribute to drought priming-enhanced Heat Tolerance in cool-season grass species.
Luis E Castaneda - One of the best experts on this subject based on the ideXlab platform.
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gut microbiota of drosophila subobscura contributes to its Heat Tolerance and is sensitive to transient thermal stress
Frontiers in Microbiology, 2021Co-Authors: Angelica Jaramillo, Luis E CastanedaAbstract:The gut microbiota can contribute to host physiology leading to an increase of resistance to abiotic stress conditions. For instance, temperature has profound effects on ectotherms, and the role of the gut microbiota on the thermal Tolerance of ectotherms is a matter of recent research. However, most of these studies have been focused on single static temperatures instead of evaluating thermal Tolerance in a wide range of stressful temperatures. Additionally, there is evidence supporting that the gut microbiota is sensitive to environmental temperature, which induces changes in its composition and diversity. These studies have evaluated the effects of thermal acclimation (>2 weeks) on the gut microbiota, but we know little about the impact of transient thermal stress on the composition and diversity of the gut microbiota. Thus, we investigated the role of the gut microbiota on the Heat Tolerance of Drosophila subobscura by measuring the Heat Tolerance of conventional and axenic flies exposed to different Heat stressful temperatures (35, 36, 37, and 38°C) and estimating the Heat Tolerance landscape for both microbiota treatments. Conventional flies exposed to mild Heat conditions exhibited higher thermal Tolerance than axenic flies, whereas at higher stressful temperatures there were no differences between axenic and conventional flies. We also assessed the impact of transient Heat stress on the taxonomical abundance, diversity, and community structure of the gut microbiota, comparing non-stressed flies (exposed to 21°C) and Heat-stressed flies (exposed to 34°C) from both sexes. Bacterial diversity indices, bacterial abundances, and community structure changed between non-stressed and Heat-stressed flies, and this response was sex-dependent. In general, our findings provide evidence that the gut microbiota influences Heat Tolerance and that Heat stress modifies the gut microbiota at the taxonomical and structural levels. These results demonstrate that the gut microbiota contributes to Heat Tolerance and is also highly sensitive to transient Heat stress, which could have important consequences on host fitness, population risk extinction, and the vulnerability of ectotherms to current and future climatic conditions.
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gut microbiota of drosophila subobscura contributes to its Heat Tolerance but is sensitive to transient thermal stress
bioRxiv, 2021Co-Authors: Angelica Jaramillo, Luis E CastanedaAbstract:Global warming impacts animal fitness, leading to increasing extinction risk in ectotherm species. Gut microbiota can contribute to host physiology leading to an increase of resistance to abiotic stress conditions. Temperature has profound effects on ectotherms and gut microbiota can influence cold and Heat Tolerance of ectotherm species. Additionally, the gut microbiota is sensitive to environmental temperature, which induces changes in its composition and diversity. Here, we investigated the role of the gut microbiota on Heat Tolerance of Drosophila subobscura comparing the knockdown time between conventional and axenic, and we also assessed the impact of Heat stress on the diversity and community structure of the gut microbiota, comparing non-stressed and Heat-stressed flies. Our findings provide evidence that gut microbiota influences Heat Tolerance, and that Heat stress modifies the gut microbiota at taxonomical and structural levels. These results demonstrate that gut microbiota contributes to Heat Tolerance but it is also highly sensitive to transient Heat stress, which could have important effects on host fitness, population risk extinction, and vulnerability of ectotherms to current and future climatic conditions.
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correlated evolution between Heat Tolerance and thermal performance curves in drosophila subobscura
bioRxiv, 2019Co-Authors: Andres Mesas, Angelica Jaramillo, Luis E CastanedaAbstract:Global warming imposes important challenges for ectotherm organisms, which can avoid the negative effects of thermal stress via evolutionary adaptation of their upper thermal limits (CTmax). In this sense, the estimation of CTmax and its evolutionary capacity is crucial to determine the vulnerability of natural populations to climate change. However, these estimates depend on the thermal stress intensity and it is not completely clear whether this thermal stress intensity can impact the evolutionary response of CTmax and thermal reaction norms (i.e. thermal performance curve, TPC). Here we performed an evolutionary experiment by selecting high Heat Tolerance using acute and chronic thermal stress in Drosophila subobscura. After artificial selection, we found that knockdown temperatures (a CTmax proxy) evolved in selected lines compared to control lines, whereas the realized heritability and evolutionary rate change of Heat Tolerance did not differ between acute-selected and chronic-selected lines. From TPC analysis, we found acute-selected lines evolved a higher optimal performance temperature (Topt) compared to acute-control lines, whereas this TPC parameter was not different between chronic-selected and chronic-control lines. The evolutionary response of Topt caused a displacement of entire TPC to high temperatures suggesting a shared genetic architecture between Heat Tolerance and high-temperature performance, which only arose in the acute-selected lines. In conclusion, thermal stress intensity has important effects on the evolution of thermal physiology in ectotherms, indicating that different thermal scenarios conduce to similar evolutionary responses of Heat Tolerance but do not for thermal performance. Therefore, thermal stress intensity could have important consequences on the estimations of the vulnerability of ectotherms to global warming.
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evolutionary potential of thermal preference and Heat Tolerance in drosophila subobscura
Journal of Evolutionary Biology, 2019Co-Authors: Luis E Castaneda, Valeria Romerosoriano, Andres Mesas, Derek A Roff, Mauro SantosAbstract:Evolutionary change of thermal traits (i.e., Heat Tolerance and behavioural thermoregulation) is one of the most important mechanisms exhibited by organisms to respond to global warming. However, the evolutionary potential of Heat Tolerance, estimated as narrow-sense heritability, depends on the methodology employed. An alternative adaptive mechanism to buffer extreme temperatures is behavioural thermoregulation, although the association between Heat Tolerance and thermal preference is not clearly understood. We suspect that methodological effects associated with the duration of Heat stress during thermal Tolerance assays are responsible for missing this genetic association. To test this hypothesis, we estimated the heritabilities and genetic correlations for thermal traits in Drosophila subobscura, using high-temperature static and slow ramping assays. We found that heritability for Heat Tolerance was higher in static assays (h2 = 0.134) than in slow ramping assays (h2 = 0.084), suggesting that fast assays may provide a more precise estimation of the genetic variation of Heat Tolerance. In addition, thermal preference exhibited a low heritability (h2 = 0.066), suggesting a reduced evolutionary response for this trait. We also found that the different estimates of Heat Tolerance and thermal preference were not genetically correlated, regardless of how Heat Tolerance was estimated. In conclusion, our data suggest that these thermal traits can evolve independently in this species. In agreement with previous evidence, these results indicate that methodology may have an important impact on genetic estimates of Heat Tolerance and that fast assays are more likely to detect the genetic component of Heat Tolerance.
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Heat Tolerance in drosophila subobscura along a latitudinal gradient contrasting patterns between plastic and genetic responses
Evolution, 2015Co-Authors: Luis E Castaneda, Enrico L Rezende, Mauro SantosAbstract:Susceptibility to global warming relies on how thermal Tolerances respond to increasing temperatures through plasticity or evolution. Climatic adaptation can be assessed by examining the geographic variation in thermal-related traits. We studied latitudinal patterns in Heat Tolerance in Drosophila subobscura reared at two temperatures. We used four static stressful temperatures to estimate the thermal death time (TDT) curves, and two ramping assays with fast and slow Heating rates. Thermal death time curves allow estimation of the critical thermal maximum (CT(max)), by extrapolating to the temperature that would knock down the flies almost "instantaneously," and the thermal sensitivity to increasing stressful temperatures. We found a positive latitudinal cline for CT(max), but no clinal pattern for knockdown temperatures estimated from the ramping assays. Although high-latitude populations were more tolerant to an acute Heat stress, they were also more sensitive to prolonged exposure to less stressful temperatures, supporting a trade-off between acute and chronic Heat Tolerances. Conversely, developmental plasticity did not affect CT(max) but increased the Tolerance to chronic Heat exposition. The patterns observed from the TDT curves help to understand why the relationship between Heat Tolerance and latitude depends on the methodology used and, therefore, these curves provide a more complete and reliable measurement of Heat Tolerance.
Salvador Herrandoperez - One of the best experts on this subject based on the ideXlab platform.
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water deprivation drives intraspecific variability in lizard Heat Tolerance
Basic and Applied Ecology, 2020Co-Authors: Salvador Herrandoperez, Josabel Belliure, Francisco Ferriyanez, Matthijs P Van Den Burg, Wouter Beukema, Miguel B Araujo, J Terblanche, David R VieitesAbstract:Abstract Quantifying intraspecific variation in Heat Tolerance is critical to understand how species respond to climate change. In a previous study, we recorded variability in critical thermal maxima (CTmax) by 3 °C among populations of small Iberian lizard species, which could substantially influence predictions of climate-driven activity restriction. Here, we undertake experiments to examine whether we could reproduce similar levels of Heat-Tolerance variability in response to water deficit. We hypothesized that deprivation of drinking water should increase variability in CTmax between populations more than deprivation of food under the theoretical expectation that the variation of the more limiting resource must trigger stronger variation in physiological performance. We measured CTmax after manipulating availability of live prey and drinking water in two populations of an arid and a mesic lizard species from the Iberian Peninsula. We quantified a mean CTmax across all studied lizards of 44.2 °C ± 0.2 SE for the arid species and 41.7 °C ± 0.3 SE for the mesic species. Using multimodel inference, we found that water deprivation (combined with food supply) caused population differences in CTmax by 3 to 4 °C which were two to three times wider than population differences due to food deprivation (combined with water supply) or to food and water provision. To highlight the need for more thermo-hydroregulatory research, we examined bias in research effort towards thermal versus hydric environmental effects on Heat Tolerance through a systematic literature review. We show that environmental temperature has been used five times more frequently than precipitation in ecological studies of Heat Tolerance of terrestrial species. Studies linking thermal Tolerance of ectotherms to the interplay of air temperature and water availability are needed in the face of projected increases in aridity and drought in the 21st century, because the balance of body temperature and water resources are functionally interlinked.
David R Vieites - One of the best experts on this subject based on the ideXlab platform.
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water deprivation drives intraspecific variability in lizard Heat Tolerance
Basic and Applied Ecology, 2020Co-Authors: Salvador Herrandoperez, Josabel Belliure, Francisco Ferriyanez, Matthijs P Van Den Burg, Wouter Beukema, Miguel B Araujo, J Terblanche, David R VieitesAbstract:Abstract Quantifying intraspecific variation in Heat Tolerance is critical to understand how species respond to climate change. In a previous study, we recorded variability in critical thermal maxima (CTmax) by 3 °C among populations of small Iberian lizard species, which could substantially influence predictions of climate-driven activity restriction. Here, we undertake experiments to examine whether we could reproduce similar levels of Heat-Tolerance variability in response to water deficit. We hypothesized that deprivation of drinking water should increase variability in CTmax between populations more than deprivation of food under the theoretical expectation that the variation of the more limiting resource must trigger stronger variation in physiological performance. We measured CTmax after manipulating availability of live prey and drinking water in two populations of an arid and a mesic lizard species from the Iberian Peninsula. We quantified a mean CTmax across all studied lizards of 44.2 °C ± 0.2 SE for the arid species and 41.7 °C ± 0.3 SE for the mesic species. Using multimodel inference, we found that water deprivation (combined with food supply) caused population differences in CTmax by 3 to 4 °C which were two to three times wider than population differences due to food deprivation (combined with water supply) or to food and water provision. To highlight the need for more thermo-hydroregulatory research, we examined bias in research effort towards thermal versus hydric environmental effects on Heat Tolerance through a systematic literature review. We show that environmental temperature has been used five times more frequently than precipitation in ecological studies of Heat Tolerance of terrestrial species. Studies linking thermal Tolerance of ectotherms to the interplay of air temperature and water availability are needed in the face of projected increases in aridity and drought in the 21st century, because the balance of body temperature and water resources are functionally interlinked.