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Attakorn Palasuwan - One of the best experts on this subject based on the ideXlab platform.

  • inhibitory activity of Heinz Body induction in vitro antioxidant model and tannin concentration of thai mimosaceous plant extracts
    Journal of Medicinal Plants Research, 2012
    Co-Authors: Tanasorn Tunsaringkarn, Suphan Soogarun, Anusorn Rungsiyothin, Attakorn Palasuwan
    Abstract:

    Medicinal plants have been used for traditional treatment and known to possess antioxidant activities that may help to reduce the risk of oxidative stress-related diseases. Chemically-induced oxidative hemolysis is usually associated with the formation of Heinz bodies-insoluble precipitates within red blood cells, indicating the oxidative destruction of hemoglobin. In the present study, antioxidant activity was investigated by Heinz Body induction in vitro model for providing preliminary data selection of plant extracts compared to tannin concentration in Thai mimosaceous plant extracts. Twenty-one Thai mimosaceous plants, extracted by ethanol, were screened for tannin concentration and antioxidant activity of inhibitory Heinz Body induction under in vitro condition. The study showed that the percentage of Heinz Body inhibition activity from Parkia speciosa seed coat was the highest and followed by Xylia xylocarpa bark, P. speciosa pericarp, and Entada rheedii seed coat, respectively with inhibition concentration25 (IC25) of 2.68, 15.71, and 28.14 mg/ml, respectively. In addition, X. xylocarpa bark, E. rheedii seed coat and P. speciosa Hassk. seed coat also contained high tannin concentration. The percentage of Heinz Body inhibition activity from plant extracts at dilution 1: 2 and 1: 10 (50.00 and 10.00 mg/ml) were shown correlation with tannin concentration (p < 0.01, r = 0.658 and p < 0.05, r= 0.536, respectively). This study also found that tannin could be found in all plant extracts and also inhibited the Heinz Body induction at the minimum level of 0.625 mg/ml. Mimosaceous plants contained antioxidant activity demonstrated by Heinz Body inhibition activity and most of the plant extracts with high Heinz Body inhibition activities might be caused by high tannin concentration. This data would be valuable sources for searching potent medicine in the future.   Key words: Heinz Body inhibition, antioxidant, mimosaceous plant.

  • inhibition of Heinz Body induction in an in vitro model and total antioxidant activity of medicinal thai plants
    Asian Pacific Journal of Cancer Prevention, 2005
    Co-Authors: Attakorn Palasuwan, Suphan Soogarun, Tamaporn Lertlum, Paweena Pradniwat, Viroj Wiwanitkit
    Abstract:

    Herbs have been used for medicinal purposes for centuries and known to possess antioxidant properties that may help to reduce the risk of chronic diseases, cardiovascular disease, and cancer. We screen aqueous extracts from 20 medicinal plants in Thailand that were believed to possess anti-tumor activity, help immune-stimulating property and maintain blood stasis. The antioxidant activities were investigated in two bioassays. Firstly, we demonstrated inhibition of Heinz bodies induction caused by oxidants under in vitro condition. The percentages of Heinz Body inhibition activity in plant extracts from Terminalia citrina, Cassia timoriensis, and Derris elliptica were the highest followed by Anamirta cocculus, and Oroxylum indicum respectively. In addition, we investigated total antioxidant activity in plant extracts by improved ABTS radical cation decolorization assay. The total antioxidant activity of the extract from Terminalia citrina was also the highest activity followed by Ficus pubigera, Derris elliptica, Anamirta cocculus, Caesalpinia sappan, and Oroxylum indicum respectively. Our results suggest medicinal Thai plants as valuable sources of antioxidants, which may have a potential anti-carcinogenic activity.

Suphan Soogarun - One of the best experts on this subject based on the ideXlab platform.

  • inhibitory activity of Heinz Body induction in vitro antioxidant model and tannin concentration of thai mimosaceous plant extracts
    Journal of Medicinal Plants Research, 2012
    Co-Authors: Tanasorn Tunsaringkarn, Suphan Soogarun, Anusorn Rungsiyothin, Attakorn Palasuwan
    Abstract:

    Medicinal plants have been used for traditional treatment and known to possess antioxidant activities that may help to reduce the risk of oxidative stress-related diseases. Chemically-induced oxidative hemolysis is usually associated with the formation of Heinz bodies-insoluble precipitates within red blood cells, indicating the oxidative destruction of hemoglobin. In the present study, antioxidant activity was investigated by Heinz Body induction in vitro model for providing preliminary data selection of plant extracts compared to tannin concentration in Thai mimosaceous plant extracts. Twenty-one Thai mimosaceous plants, extracted by ethanol, were screened for tannin concentration and antioxidant activity of inhibitory Heinz Body induction under in vitro condition. The study showed that the percentage of Heinz Body inhibition activity from Parkia speciosa seed coat was the highest and followed by Xylia xylocarpa bark, P. speciosa pericarp, and Entada rheedii seed coat, respectively with inhibition concentration25 (IC25) of 2.68, 15.71, and 28.14 mg/ml, respectively. In addition, X. xylocarpa bark, E. rheedii seed coat and P. speciosa Hassk. seed coat also contained high tannin concentration. The percentage of Heinz Body inhibition activity from plant extracts at dilution 1: 2 and 1: 10 (50.00 and 10.00 mg/ml) were shown correlation with tannin concentration (p < 0.01, r = 0.658 and p < 0.05, r= 0.536, respectively). This study also found that tannin could be found in all plant extracts and also inhibited the Heinz Body induction at the minimum level of 0.625 mg/ml. Mimosaceous plants contained antioxidant activity demonstrated by Heinz Body inhibition activity and most of the plant extracts with high Heinz Body inhibition activities might be caused by high tannin concentration. This data would be valuable sources for searching potent medicine in the future.   Key words: Heinz Body inhibition, antioxidant, mimosaceous plant.

  • Inhibitory activity of Heinz Body induction in vitro antioxidant model and tannin concentration of Thai mimosaceous plant extracts
    Journal of Medicinal Plants Research, 2012
    Co-Authors: Tanasorn Tunsaringkarn, Suphan Soogarun, Anusorn Rungsiyothin
    Abstract:

    Medicinal plants have been used for traditional treatment and known to possess antioxidant activities that may help to reduce the risk of oxidative stress-related diseases. Chemically-induced oxidative hemolysis is usually associated with the formation of Heinz bodies-insoluble precipitates within red blood cells, indicating the oxidative destruction of hemoglobin. In the present study, antioxidant activity was investigated by Heinz Body induction

  • inhibition of Heinz Body induction in an in vitro model and total antioxidant activity of medicinal thai plants
    Asian Pacific Journal of Cancer Prevention, 2005
    Co-Authors: Attakorn Palasuwan, Suphan Soogarun, Tamaporn Lertlum, Paweena Pradniwat, Viroj Wiwanitkit
    Abstract:

    Herbs have been used for medicinal purposes for centuries and known to possess antioxidant properties that may help to reduce the risk of chronic diseases, cardiovascular disease, and cancer. We screen aqueous extracts from 20 medicinal plants in Thailand that were believed to possess anti-tumor activity, help immune-stimulating property and maintain blood stasis. The antioxidant activities were investigated in two bioassays. Firstly, we demonstrated inhibition of Heinz bodies induction caused by oxidants under in vitro condition. The percentages of Heinz Body inhibition activity in plant extracts from Terminalia citrina, Cassia timoriensis, and Derris elliptica were the highest followed by Anamirta cocculus, and Oroxylum indicum respectively. In addition, we investigated total antioxidant activity in plant extracts by improved ABTS radical cation decolorization assay. The total antioxidant activity of the extract from Terminalia citrina was also the highest activity followed by Ficus pubigera, Derris elliptica, Anamirta cocculus, Caesalpinia sappan, and Oroxylum indicum respectively. Our results suggest medicinal Thai plants as valuable sources of antioxidants, which may have a potential anti-carcinogenic activity.

Jesse A. Fallon - One of the best experts on this subject based on the ideXlab platform.

  • Short Communication A PRACTICAL QUANTIFICATION METHOD FOR Heinz BODIES IN BIRDS APPLICABLE TO RAPID RESPONSE FIELD SCENARIOS
    2016
    Co-Authors: Jesse A. Fallon, William Y A. Hopkins, Lee Foxz
    Abstract:

    Abstract—Oil-induced oxidative injury to red blood cells results in Heinz Body hemolytic anemia. Here, we evaluated three Heinz Body staining techniques in brown pelican (Pelecanus occidentalis) blood. Using a range of in vitro acetylphenylhydrazine incubations, we validated a field-adapted technique against laboratory wet-mounts and verified the stability of this technique for one month following preparation. Employing this technique during petrochemical spill responses allows for delays between sample collection and analysis

  • Short Communication A PRACTICAL QUANTIFICATION METHOD FOR Heinz BODIES IN BIRDS APPLICABLE TO RAPID RESPONSE FIELD SCENARIOS
    2013
    Co-Authors: Jesse A. Fallon, William A. Hopkins
    Abstract:

    Oil-induced oxidative injury to red blood cells results in Heinz Body hemolytic anemia. Here, we evaluated three Heinz Body staining techniques in brown pelican (Pelecanus occidentalis) blood. Using a range of in vitro acetylphenylhydrazine incubations, we validated a field-adapted technique against laboratory wet-mounts and verified the stability of this technique for one month following preparation. Employing this technique during petrochemical spill responses allows for delays between sample collection and analysis. Environ. Toxicol. Chem. 2013;32:401–405. # 2012 SETAC Keywords—Heinz Body Oxidative hemolysis Hemolytic anemia Pelecanus occidentalis

  • A practical quantification method for Heinz bodies in birds applicable to rapid response field scenarios
    Environmental toxicology and chemistry, 2012
    Co-Authors: Jesse A. Fallon, William A. Hopkins, Lee Fox
    Abstract:

    Oil-induced oxidative injury to red blood cells results in Heinz Body hemolytic anemia. Here, we evaluated three Heinz Body staining techniques in brown pelican (Pelecanus occidentalis) blood. Using a range of in vitro acetylphenylhydrazine incubations, we validated a field-adapted technique against laboratory wet-mounts and verified the stability of this technique for one month following preparation. Employing this technique during petrochemical spill responses allows for delays between sample collection and analysis.

Viroj Wiwanitkit - One of the best experts on this subject based on the ideXlab platform.

  • inhibition of Heinz Body induction in an in vitro model and total antioxidant activity of medicinal thai plants
    Asian Pacific Journal of Cancer Prevention, 2005
    Co-Authors: Attakorn Palasuwan, Suphan Soogarun, Tamaporn Lertlum, Paweena Pradniwat, Viroj Wiwanitkit
    Abstract:

    Herbs have been used for medicinal purposes for centuries and known to possess antioxidant properties that may help to reduce the risk of chronic diseases, cardiovascular disease, and cancer. We screen aqueous extracts from 20 medicinal plants in Thailand that were believed to possess anti-tumor activity, help immune-stimulating property and maintain blood stasis. The antioxidant activities were investigated in two bioassays. Firstly, we demonstrated inhibition of Heinz bodies induction caused by oxidants under in vitro condition. The percentages of Heinz Body inhibition activity in plant extracts from Terminalia citrina, Cassia timoriensis, and Derris elliptica were the highest followed by Anamirta cocculus, and Oroxylum indicum respectively. In addition, we investigated total antioxidant activity in plant extracts by improved ABTS radical cation decolorization assay. The total antioxidant activity of the extract from Terminalia citrina was also the highest activity followed by Ficus pubigera, Derris elliptica, Anamirta cocculus, Caesalpinia sappan, and Oroxylum indicum respectively. Our results suggest medicinal Thai plants as valuable sources of antioxidants, which may have a potential anti-carcinogenic activity.

Lee Fox - One of the best experts on this subject based on the ideXlab platform.

  • A practical quantification method for Heinz bodies in birds applicable to rapid response field scenarios
    Environmental toxicology and chemistry, 2012
    Co-Authors: Jesse A. Fallon, William A. Hopkins, Lee Fox
    Abstract:

    Oil-induced oxidative injury to red blood cells results in Heinz Body hemolytic anemia. Here, we evaluated three Heinz Body staining techniques in brown pelican (Pelecanus occidentalis) blood. Using a range of in vitro acetylphenylhydrazine incubations, we validated a field-adapted technique against laboratory wet-mounts and verified the stability of this technique for one month following preparation. Employing this technique during petrochemical spill responses allows for delays between sample collection and analysis.