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Annie Levieux - One of the best experts on this subject based on the ideXlab platform.
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early immunodiagnosis of caprine fasciolosis using the specific f2 antigen in a passive Hemagglutination Test
Veterinary Parasitology, 1994Co-Authors: Didier Levieux, Annie LevieuxAbstract:Abstract An improved Hemagglutination (HA) Test using the purified specific f2 antigen of Fasciola hepatica has been evaluated with respect to its potential use in the diagnosis of caprine fasciolosis. Following experimental infection of 1-year-old goats with a single heavy infection of 300 metacercariae, f2-specific antibodies were detected 2–3 weeks after infection and increased steadily to reach a maximum titer 9 weeks after infection, after which the antibody level declined. In animals receiving multiple infections of a lower dose of 50 metacercariae given at weekly intervals for 6 weeks, f2-specific antibodies were detected 3 weeks after infection and increased to reach a plateau 11 weeks after infection which was maintained until the end of the experiment (15 weeks after infection). Depending on animals and groups, eggs appeared in the feces between 7 and 9 weeks after infection. The HA Test may provide valuable information about the early detection of caprine fasciolosis particularly during the prepatent period.
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early immunodiagnosis of bovine fascioliasis using the specific antigen f2 in a passive Hemagglutination Test
Veterinary Parasitology, 1992Co-Authors: Didier Levieux, Annie Levieux, Christian Mage, Annie VenienAbstract:Abstract An improved Hemagglutination (HA) Test using the purified specific f2 antigen of Fasciola hepatica has been evaluated with regard to its potential use for early diagnosis of infection. On experimental infection of beef calves 6–8 months of age, f2-specific antibodies were detected 2–4 weeks after inoculation and persisted at high levels during the 28 week experimental period. On natural infection of dairy calves, 6–9 months of age, allowed to graze in infected fields from April to June, 48% of the animals were positive in July although coproscopic analyses were negative. In November all the calves were HA positive but only 24% of them excreted F. hepatica eggs. Beef calves 1–3 months of age allowed to graze with their dams in infected fields continued to suckle their dams and were weakly infected according to HA results. This weak infection was not detected by coproscopical analysis. Colostral f2-specific antibodies persisted for approximately 6 months in the serum of dairy calves allowed to suckle their dams immediately after birth.
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an improved passive Hemagglutination Test for the serological diagnosis of bovine fascioliasis using the specific antigen f2
Veterinary Parasitology, 1992Co-Authors: Didier Levieux, Annie Levieux, Annie VenienAbstract:Abstract Optimum conditions for coupling the specific antigen f2 of Fasciola hepatica to sheep red cells and for the preparation of control cells coated with an unrelated protein are described. With a careful selection of donor sheep for erythrocytes, the problem of anti-species antibodies in bovine sera has been overcome and results may be obtained within 1 h as only one step is required in the assay system. Incorporation of a concentration of 25 mM CaCl2 in the diluent achieved increased stability of the agglutinates and enabled a more precise estimation of the end-point to be made. Analyses performed on bovine sera obtained at slaughter provided results in good agreement with the presence of flukes or fascioliasis lesions in livers at routine slaughter inspection. The developed assay is simpler, faster, more sensitive (P
Didier Levieux - One of the best experts on this subject based on the ideXlab platform.
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early immunodiagnosis of caprine fasciolosis using the specific f2 antigen in a passive Hemagglutination Test
Veterinary Parasitology, 1994Co-Authors: Didier Levieux, Annie LevieuxAbstract:Abstract An improved Hemagglutination (HA) Test using the purified specific f2 antigen of Fasciola hepatica has been evaluated with respect to its potential use in the diagnosis of caprine fasciolosis. Following experimental infection of 1-year-old goats with a single heavy infection of 300 metacercariae, f2-specific antibodies were detected 2–3 weeks after infection and increased steadily to reach a maximum titer 9 weeks after infection, after which the antibody level declined. In animals receiving multiple infections of a lower dose of 50 metacercariae given at weekly intervals for 6 weeks, f2-specific antibodies were detected 3 weeks after infection and increased to reach a plateau 11 weeks after infection which was maintained until the end of the experiment (15 weeks after infection). Depending on animals and groups, eggs appeared in the feces between 7 and 9 weeks after infection. The HA Test may provide valuable information about the early detection of caprine fasciolosis particularly during the prepatent period.
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early immunodiagnosis of bovine fascioliasis using the specific antigen f2 in a passive Hemagglutination Test
Veterinary Parasitology, 1992Co-Authors: Didier Levieux, Annie Levieux, Christian Mage, Annie VenienAbstract:Abstract An improved Hemagglutination (HA) Test using the purified specific f2 antigen of Fasciola hepatica has been evaluated with regard to its potential use for early diagnosis of infection. On experimental infection of beef calves 6–8 months of age, f2-specific antibodies were detected 2–4 weeks after inoculation and persisted at high levels during the 28 week experimental period. On natural infection of dairy calves, 6–9 months of age, allowed to graze in infected fields from April to June, 48% of the animals were positive in July although coproscopic analyses were negative. In November all the calves were HA positive but only 24% of them excreted F. hepatica eggs. Beef calves 1–3 months of age allowed to graze with their dams in infected fields continued to suckle their dams and were weakly infected according to HA results. This weak infection was not detected by coproscopical analysis. Colostral f2-specific antibodies persisted for approximately 6 months in the serum of dairy calves allowed to suckle their dams immediately after birth.
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an improved passive Hemagglutination Test for the serological diagnosis of bovine fascioliasis using the specific antigen f2
Veterinary Parasitology, 1992Co-Authors: Didier Levieux, Annie Levieux, Annie VenienAbstract:Abstract Optimum conditions for coupling the specific antigen f2 of Fasciola hepatica to sheep red cells and for the preparation of control cells coated with an unrelated protein are described. With a careful selection of donor sheep for erythrocytes, the problem of anti-species antibodies in bovine sera has been overcome and results may be obtained within 1 h as only one step is required in the assay system. Incorporation of a concentration of 25 mM CaCl2 in the diluent achieved increased stability of the agglutinates and enabled a more precise estimation of the end-point to be made. Analyses performed on bovine sera obtained at slaughter provided results in good agreement with the presence of flukes or fascioliasis lesions in livers at routine slaughter inspection. The developed assay is simpler, faster, more sensitive (P
E Rodriguez F Ferri - One of the best experts on this subject based on the ideXlab platform.
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value of indirect Hemagglutination and coagglutination Tests for serotyping haemophilus parasuis
Journal of Clinical Microbiology, 2003Co-Authors: M L Del Rio, C B Gutierrez, E Rodriguez F FerriAbstract:An indirect Hemagglutination Test (IHA) and a coagglutination Test (CA) were evaluated using saline, boiled, and autoclaved extracts for serotyping Haemophilus parasuis. CA showed several cross-reactions, whereas IHA gave rise to specific reactions, with minor exceptions. IHA was further compared with the immunodiffusion Test (the “gold standard”) for the serotyping of 67 field isolates. As a conclusion, IHA is recommended as a useful method for sensitive and specific serotyping of H. parasuis.
Annie Venien - One of the best experts on this subject based on the ideXlab platform.
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early immunodiagnosis of bovine fascioliasis using the specific antigen f2 in a passive Hemagglutination Test
Veterinary Parasitology, 1992Co-Authors: Didier Levieux, Annie Levieux, Christian Mage, Annie VenienAbstract:Abstract An improved Hemagglutination (HA) Test using the purified specific f2 antigen of Fasciola hepatica has been evaluated with regard to its potential use for early diagnosis of infection. On experimental infection of beef calves 6–8 months of age, f2-specific antibodies were detected 2–4 weeks after inoculation and persisted at high levels during the 28 week experimental period. On natural infection of dairy calves, 6–9 months of age, allowed to graze in infected fields from April to June, 48% of the animals were positive in July although coproscopic analyses were negative. In November all the calves were HA positive but only 24% of them excreted F. hepatica eggs. Beef calves 1–3 months of age allowed to graze with their dams in infected fields continued to suckle their dams and were weakly infected according to HA results. This weak infection was not detected by coproscopical analysis. Colostral f2-specific antibodies persisted for approximately 6 months in the serum of dairy calves allowed to suckle their dams immediately after birth.
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an improved passive Hemagglutination Test for the serological diagnosis of bovine fascioliasis using the specific antigen f2
Veterinary Parasitology, 1992Co-Authors: Didier Levieux, Annie Levieux, Annie VenienAbstract:Abstract Optimum conditions for coupling the specific antigen f2 of Fasciola hepatica to sheep red cells and for the preparation of control cells coated with an unrelated protein are described. With a careful selection of donor sheep for erythrocytes, the problem of anti-species antibodies in bovine sera has been overcome and results may be obtained within 1 h as only one step is required in the assay system. Incorporation of a concentration of 25 mM CaCl2 in the diluent achieved increased stability of the agglutinates and enabled a more precise estimation of the end-point to be made. Analyses performed on bovine sera obtained at slaughter provided results in good agreement with the presence of flukes or fascioliasis lesions in livers at routine slaughter inspection. The developed assay is simpler, faster, more sensitive (P
Marcelo Gottschalk - One of the best experts on this subject based on the ideXlab platform.
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development of a new serological Test for serotyping haemophilus parasuis isolates and determination of their prevalence in north america
Journal of Clinical Microbiology, 2004Co-Authors: M Tadjine, K R Mittal, S Bourdon, Marcelo GottschalkAbstract:Haemophilus parasuis causes polyserositis in swine. Fifteen serovars have been characterized by immunodiffusion Test, but many field strains are not typeable. Isolates (n = 300) of H. parasuis from animals in North America were serotyped by a new indirect Hemagglutination Test. The Test was rapid and effective for serotyping of H. parasuis, and serovars 4, 5, 13, and 7 were the most prevalent serotypes.