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O. M. Koch - One of the best experts on this subject based on the ideXlab platform.

  • Flow cytometric analysis of protein phosphorylation in the Hematopoetic System.
    Leukemia & Lymphoma, 1998
    Co-Authors: Carsten Müller, Monika Bonmann, Uwe Cassens, O. M. Koch
    Abstract:

    Cellular growth and differentiation in blood cells are regulated by the phosphorylation status of growth factor receptors and downstream proteins. Protein kinases and phosphatases balance the homeostasis of protein phosphorylation. Various diseases are associated with alterations in these tightly regulated processes. Aberrations have been proved to be of diagnostic value and might enhance the pathophysiological insight into the origin of the disease. However, quantitation of protein phosphorylation is currently not feasible in a clinical situation.We developed a flow cytometric methodology which enables for direct investigation of protein phosphorylation in cell populations defined by multi-color flow cytometry. This assay does not only overcome drawbacks of traditional methodologies (e.g. Western blotting) but also allows quantitative analyses even in rare cell populations.We accurately examined phosphorylation levels in different cell populations of hematological interest and especially analyzed CD34+ h...

Carsten Müller - One of the best experts on this subject based on the ideXlab platform.

  • Flow cytometric analysis of protein phosphorylation in the Hematopoetic System.
    Leukemia & Lymphoma, 1998
    Co-Authors: Carsten Müller, Monika Bonmann, Uwe Cassens, O. M. Koch
    Abstract:

    Cellular growth and differentiation in blood cells are regulated by the phosphorylation status of growth factor receptors and downstream proteins. Protein kinases and phosphatases balance the homeostasis of protein phosphorylation. Various diseases are associated with alterations in these tightly regulated processes. Aberrations have been proved to be of diagnostic value and might enhance the pathophysiological insight into the origin of the disease. However, quantitation of protein phosphorylation is currently not feasible in a clinical situation.We developed a flow cytometric methodology which enables for direct investigation of protein phosphorylation in cell populations defined by multi-color flow cytometry. This assay does not only overcome drawbacks of traditional methodologies (e.g. Western blotting) but also allows quantitative analyses even in rare cell populations.We accurately examined phosphorylation levels in different cell populations of hematological interest and especially analyzed CD34+ h...

C. F. Sitzmann - One of the best experts on this subject based on the ideXlab platform.

  • MR Tomography of the Legs in Children with Disorders of the Hematopoetic System
    1992
    Co-Authors: A. Kretschmer, W. Dewes, N. Graf, U. Hau, R. Kubale, B. Kramann, C. F. Sitzmann
    Abstract:

    Unterschenkel und Knieregion von 41 Kindern mit Erkrankungen des hamatopoetischen Systems wurden im Verlauf insgesamt 119mal kernspintomographisch untersucht (T1- und T2/protonengewichtete Spin-Echo-Sequenzen, T2-Gradienten-Echo-Sequenz). Vor Therapie wiesen die Leukamien meist diffuse, unter Therapie und im Rezidiv fleckformige Knochenmarkveranderungen auf. Ihr Verhalten in der T2-Betonung erlaubte eine Diskriminierung in Infiltrate, Fibrose, Knochennekrose, Siderose und gesteigerte Hamatopoese. Bei 3 Patienten mit Verdacht auf Osteomyelitis, Ewing-Sarkom oder Coxitis war erst die MR-Tomographie richtungweisend fur die Diagnose. Um wertvolle Zeit fur den Therapiebeginn zu gewinnen, sollte die Indikation zur MR-Diagnostik bei Kindern groszugiger gestellt werden. Die Beschrankung auf eine T1-betonte Spin-Echo-Sequenz und eine T2-betonte Gradienten-Echo-Sequenz ermoglicht zudem ein Screening mehrerer Korperregionen zur Therapiekontrolle hamatopoetischer Erkrankungen.

  • MR tomography of the lower limbs of children with diseases of the hematopoietic System
    Nuklearmedizin. Nuclear medicine, 1992
    Co-Authors: A. Kretschmer, W. Dewes, N. Graf, U. Hau, R. Kubale, B. Kramann, C. F. Sitzmann
    Abstract:

    In 41 children with disorders of the Hematopoetic System 119 MR examinations of both tibiae and knees were performed (T1 and T2 spin-echo sequences and a T2 gradient-echo sequence). Before therapy bone marrow changes in leukemia were diffuse, and patchy during and after therapy. Signals in T2-weighted images were different for infiltrations, fibrosis, necrosis and siderosis. In 3 children suspected of suffering from osteomyelitis, Ewing-sarcoma or coxitis, MR examination was the first to show the correct diagnosis. Therefore in children indications for MR tomography should be handled more generously to win time for therapy. Using only a T1 spin-echo sequence and a T2 gradient-echo sequence screening of more than one region is capable of controlling bone marrow diseases.

Gökhan Keser - One of the best experts on this subject based on the ideXlab platform.

  • AB0882 Coexistence of Systemic lupus eryhematosus and ankylosing spondylitis a case report and review of the literature
    Annals of the Rheumatic Diseases, 2013
    Co-Authors: F. Tarhan, M. Argın, Gerçek Can, Mustafa Ozmen, Gökhan Keser
    Abstract:

    Background Systemic lupus erythematosus (SLE) is a chronic inflammatory disease of unknown etiology that may affect the skin, joints, kidneys, lungs, nervous System, serous membranes, and/or other organs of the body. Ankylosing spondylitis (AS) is a chronic inflammatory disease of the axial skeleton manifested by inflammatory back pain and progressive stiffness of the spine. These two autoimmune rheumatologic diseases, which have different etiopathogenesis, as well as diverse clinical and genetic characteristics, are seldom seen together. Objectives Hereby, we present a 55-year-old female patient, being followed up with the diagnosis of SLE with locomotor, skin, renal and Hematopoetic System involvement for eight years, who also suffered from inflammatory low back pain approximately for the last four months. Results Sacroiliac magnetic resonance imaging (MRI) confirmed the presence of bilateral sacroiliitis and HLA-B27 was positive. So, additional diagnosis of AS was made eight years after the diagnosis of SLE. Her laboratory test results were as follows: white blood cell count 3.85/μl (4.60-10.2), hemoglobin10.4g/dL (12.2-18.1), antinuclear antibody test 1/320 granular, anti-double-stranded DNA positive, Complement 3 65 mg/dl (83-193), Complement 4: 3 mg/dl (15-57). Renal biopsy was performed, and histopathological examination showed membranous glomerulonephritis, consistent with class V lupus nephritis. Inflammatory low back pain responded to treatment with non-steroidal anti-inflammatory drugs. Conclusions Including the present case, most of the reported cases of SLE and AS coexistance are females and generally SLE precedes the occurence of AS[1]. The present case is also notable with having MRI confirmation of bilateral active sacroiliitis with bone marrow edema. The coexistance of these two diseases with different genetic bacgrounds and clinical symptoms may implicate the importance of shared enviromental factors. 1. Mrabet D, Rekik S, Sahli H, Trojet S, Cheour I, Eleuch M et al (2011) Ankylosing spondylitis in female Systemic lupus erythematosus: a rare combination. Lupus 20(14):777-8 Disclosure of Interest None Declared

Monika Bonmann - One of the best experts on this subject based on the ideXlab platform.

  • Flow cytometric analysis of protein phosphorylation in the Hematopoetic System.
    Leukemia & Lymphoma, 1998
    Co-Authors: Carsten Müller, Monika Bonmann, Uwe Cassens, O. M. Koch
    Abstract:

    Cellular growth and differentiation in blood cells are regulated by the phosphorylation status of growth factor receptors and downstream proteins. Protein kinases and phosphatases balance the homeostasis of protein phosphorylation. Various diseases are associated with alterations in these tightly regulated processes. Aberrations have been proved to be of diagnostic value and might enhance the pathophysiological insight into the origin of the disease. However, quantitation of protein phosphorylation is currently not feasible in a clinical situation.We developed a flow cytometric methodology which enables for direct investigation of protein phosphorylation in cell populations defined by multi-color flow cytometry. This assay does not only overcome drawbacks of traditional methodologies (e.g. Western blotting) but also allows quantitative analyses even in rare cell populations.We accurately examined phosphorylation levels in different cell populations of hematological interest and especially analyzed CD34+ h...