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Allen A. Smith - One of the best experts on this subject based on the ideXlab platform.
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Bismuth Hematoxylin stain for arginine residues.
Biotechnic & Histochemistry, 2009Co-Authors: John B. Roach, Allen A. SmithAbstract:Bismuth ions complex with Hematoxylin oxidized by sodium iodate to form a dark blue dye that stains structures with high arginine content. In citrate buffer at pH 5.2, staining is confined to cell nuclei and myelin sheaths. Extraction of nucleic acids has little effect on the stain. Blockade of the guanidino groups of arginine completely abolishes staining.
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Use of an improved zirconyl Hematoxylin stain in the diagnosis of Barrett's esophagus.
Journal of Cellular and Molecular Medicine, 2004Co-Authors: Jeannette M. Mcnulty, Michael J. Kambour, Allen A. SmithAbstract:Barrett's esophagus is a precancerous condition characterized by replacement of the normal stratified squamous epithelium by a simple columnar epithelium with goblet cells that secrete an acidic mucin. As originally formulated, fresh solutions of zirconyl Hematoxylin stain goblet cells poorly. An improved formula, quintupling the amount of oxidant, yields zirconyl Hematoxylin solutions that stain goblet cells darkly even when fresh. The improved zirconyl Hematoxylin can be used in place of alcian blue in the diagnosis of Barrett's esophagus. The ingredients of zirconyl Hematoxylin are always readily available and are generally recognized as safe.
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Zirconyl Hematoxylin vs. alcian blue staining in a case of extramammary Paget's disease
Journal of Cutaneous Pathology, 2003Co-Authors: Allen A. Smith, Timothy M. SilverAbstract:Background: Despite its occasional unavailability, alcian blue has long been the stain of choice for the pathognomonic mucin-secreting cells of extramammary Paget's disease. Zirconyl Hematoxylin is a recently developed substitute for alcian blue, which is made from common laboratory materials. Methods: We have stained sections from three different areas of the same case of extramammary Paget's disease with zirconyl Hematoxylin, destained in 7% HCl in ethanol, restained with alcian blue at pH 2.5, destained in 2.5% trifluoroacetic acid in dichloromethane, and restained with zirconyl Hematoxylin. Results: Each staining procedure stained the same cells. Conclusions: Zirconyl Hematoxylin is a useful alternative to alcian blue in the diagnosis of extramammary Paget's disease.
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Zirconyl Hematoxylin: a new stain for acidic mucins.
Biotechnic & Histochemistry, 2000Co-Authors: Allen A. SmithAbstract:Most stains for acidic mucins are time-consuming to prepare and have poor stability. Zirconyl Hematoxylin is easily prepared and works for a year or more. It is made by adding 5 ml freshly-made 0.1% aqueous sodium iodate, 400 mg zirconyl chloride oc-tahydrate, and 40 ml 25% aqueous glycerol, in that order, to 100 mg of Hematoxylin in 5 ml of absolute ethanol and stirring for 5 min. Stain 10 min and do not “blue” the stain. Chlorazole black or kernechtrot and fast green are good counterstains. Zirconyl Hematoxylin stains acidic mucins violet or red violet, regardless of how they are fixed. It stains the same mucins as alcian blue in mouse and sheep salivary glands. It shows goblet cells in mouse rectum as well as alcian blue. It stains the same stomach regions in a lizard as alcian blue. Like alcian blue and colloidal iron, zirconyl Hematoxylin stains the mucin of cancerous prostate, but not normal prostate.
David Y Graham - One of the best experts on this subject based on the ideXlab platform.
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a new triple stain for helicobacter pylori suitable for the autostainer carbol fuchsin alcian blue Hematoxylin eosin
Archives of Pathology & Laboratory Medicine, 1998Co-Authors: Hala M T Elzimaity, H Ota, S Scott, D E Killen, David Y GrahamAbstract:OBJECTIVE To develop an inexpensive stain to simultaneously visualize gastric morphology and Helicobacter pylori. METHODS Gastric biopsies were stained with Genta stain using manual methods, and with carbol fuchsin/Alcian blue/Hematoxylin-eosin using an automatic slide stainer (Sakura DRS-601). Slides were then coded and interpreted by 2 pathologists. Helicobacter pylori was scored using a visual scale (0 [none] to 5 [maximum]). RESULTS One hundred slides were scored; H pylori was present in 64%. Carbol fuchsin/Alcian blue/Hematoxylin-eosin stain gave excellent demonstration of gastric morphology. All positive cases (score > or =2) were correctly interpreted. Thirty-six slides had a score of 1 (< or =2 bacteria per entire slide). Of these, 10 were scored negative by Genta stain and 12 were scored negative by the carbol fuchsin/Alcian blue/Hematoxylin-eosin stain (P = not significant). Hematoxylin-eosin was significantly less accurate than either of the other 2 stains (P < .02). CONCLUSION The carbol fuchsin/Alcian blue/Hematoxylin-eosin (El-Zimaity) stain is an economical stain suitable for simultaneous visualization of H pylori infection and gastric morphology.
Hala M T Elzimaity - One of the best experts on this subject based on the ideXlab platform.
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a new triple stain for helicobacter pylori suitable for the autostainer carbol fuchsin alcian blue Hematoxylin eosin
Archives of Pathology & Laboratory Medicine, 1998Co-Authors: Hala M T Elzimaity, H Ota, S Scott, D E Killen, David Y GrahamAbstract:OBJECTIVE To develop an inexpensive stain to simultaneously visualize gastric morphology and Helicobacter pylori. METHODS Gastric biopsies were stained with Genta stain using manual methods, and with carbol fuchsin/Alcian blue/Hematoxylin-eosin using an automatic slide stainer (Sakura DRS-601). Slides were then coded and interpreted by 2 pathologists. Helicobacter pylori was scored using a visual scale (0 [none] to 5 [maximum]). RESULTS One hundred slides were scored; H pylori was present in 64%. Carbol fuchsin/Alcian blue/Hematoxylin-eosin stain gave excellent demonstration of gastric morphology. All positive cases (score > or =2) were correctly interpreted. Thirty-six slides had a score of 1 (< or =2 bacteria per entire slide). Of these, 10 were scored negative by Genta stain and 12 were scored negative by the carbol fuchsin/Alcian blue/Hematoxylin-eosin stain (P = not significant). Hematoxylin-eosin was significantly less accurate than either of the other 2 stains (P < .02). CONCLUSION The carbol fuchsin/Alcian blue/Hematoxylin-eosin (El-Zimaity) stain is an economical stain suitable for simultaneous visualization of H pylori infection and gastric morphology.
A.a. Smith - One of the best experts on this subject based on the ideXlab platform.
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An improved formulation of the zirconyl Hematoxylin stain for acidic mucins.
Biotechnic & Histochemistry, 2004Co-Authors: Jm Mcnulty, A.a. SmithAbstract:Zirconyl Hematoxylin stains acidic mucins darkly and specifically using a solution of 100 mg Hematoxylin, 5 ml ethanol, 5 ml 0.5% sodium iodate, 400 mg zirconyl chloride octahydrate, and 30 ml 25% aqueous glycerol. The stain is especially advantageous for studying goblet cells and Paget cells.
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Specific staining of tissue components with metal-Hematoxylin complexes.
Micron, 2001Co-Authors: A.a. SmithAbstract:Abstract The older metal–Hematoxylin stains stain a broad spectrum of tissue components. Several recently introduced metal–Hematoxylin stains are highly selective. This selectivity is usually bought at the price of severe limitations on the choice of fixative. A very dilute (2×10 −4 M ) aluminum Hematoxylin is selective for nucleic acids in tissues fixed in organic solvents alone. Vanadate Hematoxylin is selective for basic proteins in tissues fixed in formaldehyde or mercuric salts. Bismuth Hematoxylin is selective for arginine residues and thus for histones and myelin basic protein in tissues fixed in strong acids (Bouin's fluid or SUSA fluid). Zirconyl Hematoxylin is selective for acidic mucins. Zirconyl Hematoxylin does not restrict the choice of fixative.
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Bismuth Hematoxylin staining of nucleic acids
Proceedings annual meeting Electron Microscopy Society of America, 1995Co-Authors: A.a. SmithAbstract:Hematoxylin is readily oxidized to the blue pigment hematein. Hematein can complex with a wide variety of metal salts or mordants to form mordant dyes. These dyes stain various components of animal tissues. The component stained depends on the metal in the mordant dye.Alum Hematoxylin uses trivalent aluminum ion as a mordant. At extreme dilution, requiring long staining times, alum Hematoxylin is a specific stain for nucleic acids. As usually used, alum Hematoxylin stains nucleoproteins, staining them nearly as well after the removal of the nucleic acids as before.Bismuth shows a high affinity for nucleic acids. Although all common bismuth salts are insoluble in water, sodium bismuthate reacts with an aqueous solution of Hematoxylin to produce hematein and trivalent bismuth ions. The bismuth ions complex with the hematein to form a water-soluble “bismuth Hematoxylin.” HEPES (N-[2-hydroxyethyl]piperazine- N'-2-ethanesulfonic acid), 8 mg/ml of solution, greatly increases the amount of bismuth Hematoxylin formed. Glycerol, 1/8 v/v, improves the stability of the complex.
Mehdi Bayat - One of the best experts on this subject based on the ideXlab platform.
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Electrochemical oxidation of Hematoxylin – Part 1: Experimental and theoretical studies in an aqueous acidic medium
Journal of Electroanalytical Chemistry, 2012Co-Authors: Hadi Beiginejad, Davood Nematollahi, Mehdi BayatAbstract:Abstract The electrochemical oxidation of Hematoxylin in acidic solutions has been studied using cyclic voltammetry, differential pulse voltammetry, controlled potential coulometry and digital simulation methods. The results revealed that Hematoxylin shows two oxidation–reduction peaks ( EE mechanism). In the first step Hematoxylin is converted to hematein via a two-electron process. Five possible structures were designed for the first oxidation step and optimized at the B3LYP/6-311 + G ∗∗ level of theory. Also, the electrochemical oxidation of Hematoxylin has been studied in the presence of p -toluenesulfinic acid as a nucleophile. The results showed that the electrochemically generated hematein participates in Michael addition reaction with p -toluenesulfinic acid via a novel “ E-Tautomerization - C ” mechanism and is converted to an unexpected isomer of tolylsulfonylHematoxylin. In this work, an electrochemical method for the synthesis of a new Hematoxylin derivative is also reported.
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electrochemical oxidation of Hematoxylin part 1 experimental and theoretical studies in an aqueous acidic medium
Journal of Electroanalytical Chemistry, 2012Co-Authors: Hadi Beiginejad, Davood Nematollahi, Mehdi BayatAbstract:Abstract The electrochemical oxidation of Hematoxylin in acidic solutions has been studied using cyclic voltammetry, differential pulse voltammetry, controlled potential coulometry and digital simulation methods. The results revealed that Hematoxylin shows two oxidation–reduction peaks ( EE mechanism). In the first step Hematoxylin is converted to hematein via a two-electron process. Five possible structures were designed for the first oxidation step and optimized at the B3LYP/6-311 + G ∗∗ level of theory. Also, the electrochemical oxidation of Hematoxylin has been studied in the presence of p -toluenesulfinic acid as a nucleophile. The results showed that the electrochemically generated hematein participates in Michael addition reaction with p -toluenesulfinic acid via a novel “ E-Tautomerization - C ” mechanism and is converted to an unexpected isomer of tolylsulfonylHematoxylin. In this work, an electrochemical method for the synthesis of a new Hematoxylin derivative is also reported.