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Aparna Gomes - One of the best experts on this subject based on the ideXlab platform.

  • adjuvant effects and antiserum action potentiation by a herbal compound 2 hydroxy 4 methoxy benzoic acid isolated from the root extract of the indian medicinal plant sarsaparilla Hemidesmus indicus r br
    Toxicon, 1998
    Co-Authors: M.i. Alam, Aparna Gomes
    Abstract:

    The adjuvant effect and antiserum potentiation of a compound 2-hydroxy-4-methoxy benzoic acid were explored in the present investigation. This compound, isolated and purified from the Indian medicinal plant Hemidesmus indicus R. Br, possessed antisnake venom activity. Rabbits immunized with Vipera russellii venom in the presence and absence of the compound along with Freund's complete adjuvant, produced a precipitating band in immunogel diffusion and immunogel electrophoresis. The venom neutralizing capacity of this antiserum showed positive adjuvant effects as evident by the higher neutralization capacity (lethal and hemorrhage) when compared with the antiserum raised with venom alone. The pure compound potentiated the lethal action neutralization of venom by commercial equine polyvalent snake venom antiserum in experimental models. These observations raised the possibility of the use of chemical antagonists (from herbs) against snake bite, which may provide a better protection in presence of antiserum, especially in the rural parts of India.

  • viper venom induced inflammation and inhibition of free radical formation by pure compound 2 hydroxy 4 methoxy benzoic acid isolated and purified from anantamul Hemidesmus indicus r br root extract
    Toxicon, 1998
    Co-Authors: M.i. Alam, Aparna Gomes
    Abstract:

    The present investigation explored the possible venom neutralizing effect of a pure compound (2-hydroxy-4-methoxy benzoic acid) isolated and purified from the methanolic root extract of Hemidesmus indicus R.Rr. 2-OH-4-MeO benzoic acid possessed potent anti-inflammatory, antipyretic and antioxidant properties. The compound effectively neutralized inflammation induced by Vipera russelli venom in male albino mice and reduced cotton pellet-induced granuloma in rats. The compound produced a significant fall in body temperature in yeast-induced pyrexia in rats but did not change the normothermic body temperature. The compound effectively neutralized viper venom-induced changes in serum phosphatase and transaminase activity in male albino rats. It also neutralized free radical formation as estimated by TBAPS and superoxide dismutase activities. The antisnake venom activity of the pure compound is partly mediated through the above physiological process.

  • Viper venom neutralization by Indian medicinal plant (Hemidesmus indicus and Pluchea indica) root extracts
    Phytotherapy Research, 1996
    Co-Authors: M.i. Alam, Biswajit Auddy, Aparna Gomes
    Abstract:

    The methanol root extracts of Hemidesmus indicus R. Br. and Pluchea indica (Less) were explored for the first time for neutralization of snake venom (Vipera russellii) activity. The H. indicus and P. indica root extracts significantly neutralized the viper venom-induced lethality and haemorrhagic activity in albino rat and mouse. Venom-induced coagulant and anticoagulant activity was also antagonized by both the extracts. No precipitating bands were observed between the plant extract and polyvalent snake venom antiserum. Maximum neutralization was achieved by H. indicus root extract. These observations confirmed that certain Indian medicinal plants possess significant snake venom neutralizing capacity and need further examination for their active constituents.

  • isolation purification and partial characterization of viper venom inhibiting factor from the root extract of the indian medicinal plant sarsaparilla Hemidesmus indicus r br
    Toxicon, 1994
    Co-Authors: M.i. Alam, Biswajit Auddy, Aparna Gomes
    Abstract:

    An organic acid, isolated and purified from the root extract of an Indian medicinal plant sarsaparilla Hemidesmus indicus R. Br, possessed viper venom inhibitory activity. The compound (designated HI-RVIF) was isolated by solvent extraction, silica gel column chromatography and thin layer chromatography, and was homogeneous in nature. The white needle-shaped crystals were soluble in water, methanol and chloroform and had a melting point of 155-158 degrees C and lambda max 260 nm. Spectral analysis confirmed the presence of a benzene ring, methoxy group, and hydroxyl group; the mol. wt of the compound was 168. HI-RVIF significantly antagonized viper venom-induced lethal, haemorrhagic, coagulant and anticoagulant activity in experimental rodents.

  • Isolation, purification and partial characterization of viper venom inhibiting factor from the root extract of the Indian medicinal plant sarsaparilla (Hemidesmus indicus R.Br.)
    Toxicon : official journal of the International Society on Toxinology, 1994
    Co-Authors: M.i. Alam, Biswajit Auddy, Aparna Gomes
    Abstract:

    Abstract An organic acid, isolated and purified from the root extract of an Indian medicinal plant sarsaparilla Hemidesmus indicus R.Br, possessed viper venom inhibitory activity. The compound (designated HI-RVIF) was isolated by solvent extraction, silica gel column chromatography and thin layer chromatography, and was homogeneous in nature. The white needle-shaped crystals were soluble in water, methanol and chloroform and had a melting point of 155–158°C and λ max 260 nm. spectral analysis confirmed the presence of a benzene ring, methoxy group, and hydroxyl group; the mol. wt of the compound was 168. HI-RVIF significantly antagonized viper venom-induced lethal, haemorrhagic, coagulant and anticoagulant activity in experimental rodents.

M.i. Alam - One of the best experts on this subject based on the ideXlab platform.

  • adjuvant effects and antiserum action potentiation by a herbal compound 2 hydroxy 4 methoxy benzoic acid isolated from the root extract of the indian medicinal plant sarsaparilla Hemidesmus indicus r br
    Toxicon, 1998
    Co-Authors: M.i. Alam, Aparna Gomes
    Abstract:

    The adjuvant effect and antiserum potentiation of a compound 2-hydroxy-4-methoxy benzoic acid were explored in the present investigation. This compound, isolated and purified from the Indian medicinal plant Hemidesmus indicus R. Br, possessed antisnake venom activity. Rabbits immunized with Vipera russellii venom in the presence and absence of the compound along with Freund's complete adjuvant, produced a precipitating band in immunogel diffusion and immunogel electrophoresis. The venom neutralizing capacity of this antiserum showed positive adjuvant effects as evident by the higher neutralization capacity (lethal and hemorrhage) when compared with the antiserum raised with venom alone. The pure compound potentiated the lethal action neutralization of venom by commercial equine polyvalent snake venom antiserum in experimental models. These observations raised the possibility of the use of chemical antagonists (from herbs) against snake bite, which may provide a better protection in presence of antiserum, especially in the rural parts of India.

  • viper venom induced inflammation and inhibition of free radical formation by pure compound 2 hydroxy 4 methoxy benzoic acid isolated and purified from anantamul Hemidesmus indicus r br root extract
    Toxicon, 1998
    Co-Authors: M.i. Alam, Aparna Gomes
    Abstract:

    The present investigation explored the possible venom neutralizing effect of a pure compound (2-hydroxy-4-methoxy benzoic acid) isolated and purified from the methanolic root extract of Hemidesmus indicus R.Rr. 2-OH-4-MeO benzoic acid possessed potent anti-inflammatory, antipyretic and antioxidant properties. The compound effectively neutralized inflammation induced by Vipera russelli venom in male albino mice and reduced cotton pellet-induced granuloma in rats. The compound produced a significant fall in body temperature in yeast-induced pyrexia in rats but did not change the normothermic body temperature. The compound effectively neutralized viper venom-induced changes in serum phosphatase and transaminase activity in male albino rats. It also neutralized free radical formation as estimated by TBAPS and superoxide dismutase activities. The antisnake venom activity of the pure compound is partly mediated through the above physiological process.

  • Viper venom neutralization by Indian medicinal plant (Hemidesmus indicus and Pluchea indica) root extracts
    Phytotherapy Research, 1996
    Co-Authors: M.i. Alam, Biswajit Auddy, Aparna Gomes
    Abstract:

    The methanol root extracts of Hemidesmus indicus R. Br. and Pluchea indica (Less) were explored for the first time for neutralization of snake venom (Vipera russellii) activity. The H. indicus and P. indica root extracts significantly neutralized the viper venom-induced lethality and haemorrhagic activity in albino rat and mouse. Venom-induced coagulant and anticoagulant activity was also antagonized by both the extracts. No precipitating bands were observed between the plant extract and polyvalent snake venom antiserum. Maximum neutralization was achieved by H. indicus root extract. These observations confirmed that certain Indian medicinal plants possess significant snake venom neutralizing capacity and need further examination for their active constituents.

  • isolation purification and partial characterization of viper venom inhibiting factor from the root extract of the indian medicinal plant sarsaparilla Hemidesmus indicus r br
    Toxicon, 1994
    Co-Authors: M.i. Alam, Biswajit Auddy, Aparna Gomes
    Abstract:

    An organic acid, isolated and purified from the root extract of an Indian medicinal plant sarsaparilla Hemidesmus indicus R. Br, possessed viper venom inhibitory activity. The compound (designated HI-RVIF) was isolated by solvent extraction, silica gel column chromatography and thin layer chromatography, and was homogeneous in nature. The white needle-shaped crystals were soluble in water, methanol and chloroform and had a melting point of 155-158 degrees C and lambda max 260 nm. Spectral analysis confirmed the presence of a benzene ring, methoxy group, and hydroxyl group; the mol. wt of the compound was 168. HI-RVIF significantly antagonized viper venom-induced lethal, haemorrhagic, coagulant and anticoagulant activity in experimental rodents.

  • Isolation, purification and partial characterization of viper venom inhibiting factor from the root extract of the Indian medicinal plant sarsaparilla (Hemidesmus indicus R.Br.)
    Toxicon : official journal of the International Society on Toxinology, 1994
    Co-Authors: M.i. Alam, Biswajit Auddy, Aparna Gomes
    Abstract:

    Abstract An organic acid, isolated and purified from the root extract of an Indian medicinal plant sarsaparilla Hemidesmus indicus R.Br, possessed viper venom inhibitory activity. The compound (designated HI-RVIF) was isolated by solvent extraction, silica gel column chromatography and thin layer chromatography, and was homogeneous in nature. The white needle-shaped crystals were soluble in water, methanol and chloroform and had a melting point of 155–158°C and λ max 260 nm. spectral analysis confirmed the presence of a benzene ring, methoxy group, and hydroxyl group; the mol. wt of the compound was 168. HI-RVIF significantly antagonized viper venom-induced lethal, haemorrhagic, coagulant and anticoagulant activity in experimental rodents.

Suresh Yenugu - One of the best experts on this subject based on the ideXlab platform.

  • mechanism of antibacterial action of the alcoholic extracts of Hemidesmus indicus l r br ex schult leucas aspera wild plumbago zeylanica l and tridax procumbens l r br ex schult
    Frontiers in Microbiology, 2015
    Co-Authors: Kongari Saritha, Angireddy Rajesh, K Manjulatha, Oruganti H Setty, Suresh Yenugu
    Abstract:

    Herbal products derived from Hemidesmus indicus (L.) R. Br. ex Schult, Leucas aspera (Wild.), Plumbago zeylanica L and Tridax procumbens (L.) R. Br. ex Schult. are widely used in traditional medicine.Though the extracts of these plants were found to be antimicrobial in nature and have the potential to be used in clinics, the mechanism of action of is not reported. The ethanolic extracts of Hemidesmus indicus (L.) R. Br. ex Schult (HIEE), Leucas aspera (Wild.)(Wild.)(LAEE), Plumbago zeylanica L (PZEE) and Tridax procumbens (L.) R. Br. ex Schult. (TPEE) were tested for their antibacterial activity against E. coli. Antibacterial activity was analyzed by CFU assay and the effect on the bacterial membrane by fluorescence activated cell sorting and scanning electron microscopy. LAEE, PZEE and HIEE displayed potent bacterial killing activity in a time and concentration dependent manner. TPEE did not display appreciable antibacterial activity. The antibacterial action involved disruption of membrane potential, inner membrane permeabilization , blebbing and leakage of cellular contents. Our results contribute to the understanding of the antibacterial mechanism of alcoholic extracts of the medicinal plants used in this study.

  • mechanism of antibacterial action of the alcoholic extracts of Hemidesmus indicus l r br ex schult leucas aspera wild plumbago zeylanica l and tridax procumbens l r br ex schult
    Frontiers in Microbiology, 2015
    Co-Authors: Kongari Saritha, Angireddy Rajesh, K Manjulatha, Oruganti H Setty, Suresh Yenugu
    Abstract:

    Herbal products derived from Hemidesmus indicus (L.) R. Br. ex Schult, Leucas aspera (Wild.), Plumbago zeylanica L., and Tridax procumbens (L.) R. Br. ex Schult. are widely used in traditional medicine. Though the extracts of these plants were found to be antimicrobial in nature and have the potential to be used in clinics, the mechanism of action of is not reported. The ethanolic extracts of Hemidesmus indicus (L.) R. Br. ex Schult, Hemidesmus indicus ethanolic extract (HIEE), Leucas aspera (Wild.), Leucas aspera ethanolic extract (LAEE), Plumbago zeylanica L., Plumbago zeylanica ethanolic extract (PZEE), and Tridax procumbens (L.) R. Br. ex Schult, Tridax procumbens ethanolic extract (TPEE) were tested for their antibacterial activity against E. coli. Antibacterial activity was analyzed by CFU assay and the effect on the bacterial membrane by fluorescence activated cell sorting and scanning electron microscopy. LAEE, PZEE, and HIEE displayed potent bacterial killing activity in a time and concentration dependent manner. TPEE did not display appreciable antibacterial activity. The antibacterial action involved disruption of membrane potential, inner membrane permeabilization, blebbing and leakage of cellular contents. Our results contribute to the understanding of the antibacterial mechanism of alcoholic extracts of the medicinal plants used in this study.

Oruganti H Setty - One of the best experts on this subject based on the ideXlab platform.

  • mechanism of antibacterial action of the alcoholic extracts of Hemidesmus indicus l r br ex schult leucas aspera wild plumbago zeylanica l and tridax procumbens l r br ex schult
    Frontiers in Microbiology, 2015
    Co-Authors: Kongari Saritha, Angireddy Rajesh, K Manjulatha, Oruganti H Setty, Suresh Yenugu
    Abstract:

    Herbal products derived from Hemidesmus indicus (L.) R. Br. ex Schult, Leucas aspera (Wild.), Plumbago zeylanica L and Tridax procumbens (L.) R. Br. ex Schult. are widely used in traditional medicine.Though the extracts of these plants were found to be antimicrobial in nature and have the potential to be used in clinics, the mechanism of action of is not reported. The ethanolic extracts of Hemidesmus indicus (L.) R. Br. ex Schult (HIEE), Leucas aspera (Wild.)(Wild.)(LAEE), Plumbago zeylanica L (PZEE) and Tridax procumbens (L.) R. Br. ex Schult. (TPEE) were tested for their antibacterial activity against E. coli. Antibacterial activity was analyzed by CFU assay and the effect on the bacterial membrane by fluorescence activated cell sorting and scanning electron microscopy. LAEE, PZEE and HIEE displayed potent bacterial killing activity in a time and concentration dependent manner. TPEE did not display appreciable antibacterial activity. The antibacterial action involved disruption of membrane potential, inner membrane permeabilization , blebbing and leakage of cellular contents. Our results contribute to the understanding of the antibacterial mechanism of alcoholic extracts of the medicinal plants used in this study.

  • mechanism of antibacterial action of the alcoholic extracts of Hemidesmus indicus l r br ex schult leucas aspera wild plumbago zeylanica l and tridax procumbens l r br ex schult
    Frontiers in Microbiology, 2015
    Co-Authors: Kongari Saritha, Angireddy Rajesh, K Manjulatha, Oruganti H Setty, Suresh Yenugu
    Abstract:

    Herbal products derived from Hemidesmus indicus (L.) R. Br. ex Schult, Leucas aspera (Wild.), Plumbago zeylanica L., and Tridax procumbens (L.) R. Br. ex Schult. are widely used in traditional medicine. Though the extracts of these plants were found to be antimicrobial in nature and have the potential to be used in clinics, the mechanism of action of is not reported. The ethanolic extracts of Hemidesmus indicus (L.) R. Br. ex Schult, Hemidesmus indicus ethanolic extract (HIEE), Leucas aspera (Wild.), Leucas aspera ethanolic extract (LAEE), Plumbago zeylanica L., Plumbago zeylanica ethanolic extract (PZEE), and Tridax procumbens (L.) R. Br. ex Schult, Tridax procumbens ethanolic extract (TPEE) were tested for their antibacterial activity against E. coli. Antibacterial activity was analyzed by CFU assay and the effect on the bacterial membrane by fluorescence activated cell sorting and scanning electron microscopy. LAEE, PZEE, and HIEE displayed potent bacterial killing activity in a time and concentration dependent manner. TPEE did not display appreciable antibacterial activity. The antibacterial action involved disruption of membrane potential, inner membrane permeabilization, blebbing and leakage of cellular contents. Our results contribute to the understanding of the antibacterial mechanism of alcoholic extracts of the medicinal plants used in this study.

  • beneficial effect of the administration of Hemidesmus indicus against bromobenzene induced oxidative stress in rat liver mitochondria
    Journal of Ethnopharmacology, 2010
    Co-Authors: S Gopi, Oruganti H Setty
    Abstract:

    Abstract Aim of the study To study the beneficial effect of the prior administration of an aqueous extract of Hemidesmus indicus against bromobenzene induced oxidative damage in rat liver mitochondria. Materials and methods Oxidative stress was induced in rats with bromobenzene (10 mmol/kg body wt.). The rate of respiration, P/O ratios, lipid peroxides, protein carbonyls and sulphydryls were studied. Results When the rats were administered with bromobenzene, the rate of respiration was decreased significantly and the P/O ratio was completely abolished. There was a significant increase on the levels of lipid peroxide and protein carbonyl and a significant decrease on total sulphydryl groups when compared with control. Administration of rats with an aqueous extract (100 mg/kg) prior to bromobenzene administration showed significant beneficial effects like, stimulation in respiration, prevented the rise in lipid peroxides and protein carbonyls, increased the level of sulphydryl groups back to control level. Administration of vitamin E could not reverse as effectively as Hemidesmus indicus. Conclusions This study demonstrates a good protective effect of Hemidesmus indicus against the bromobenzene induced oxidative stress.

Kongari Saritha - One of the best experts on this subject based on the ideXlab platform.

  • mechanism of antibacterial action of the alcoholic extracts of Hemidesmus indicus l r br ex schult leucas aspera wild plumbago zeylanica l and tridax procumbens l r br ex schult
    Frontiers in Microbiology, 2015
    Co-Authors: Kongari Saritha, Angireddy Rajesh, K Manjulatha, Oruganti H Setty, Suresh Yenugu
    Abstract:

    Herbal products derived from Hemidesmus indicus (L.) R. Br. ex Schult, Leucas aspera (Wild.), Plumbago zeylanica L and Tridax procumbens (L.) R. Br. ex Schult. are widely used in traditional medicine.Though the extracts of these plants were found to be antimicrobial in nature and have the potential to be used in clinics, the mechanism of action of is not reported. The ethanolic extracts of Hemidesmus indicus (L.) R. Br. ex Schult (HIEE), Leucas aspera (Wild.)(Wild.)(LAEE), Plumbago zeylanica L (PZEE) and Tridax procumbens (L.) R. Br. ex Schult. (TPEE) were tested for their antibacterial activity against E. coli. Antibacterial activity was analyzed by CFU assay and the effect on the bacterial membrane by fluorescence activated cell sorting and scanning electron microscopy. LAEE, PZEE and HIEE displayed potent bacterial killing activity in a time and concentration dependent manner. TPEE did not display appreciable antibacterial activity. The antibacterial action involved disruption of membrane potential, inner membrane permeabilization , blebbing and leakage of cellular contents. Our results contribute to the understanding of the antibacterial mechanism of alcoholic extracts of the medicinal plants used in this study.

  • mechanism of antibacterial action of the alcoholic extracts of Hemidesmus indicus l r br ex schult leucas aspera wild plumbago zeylanica l and tridax procumbens l r br ex schult
    Frontiers in Microbiology, 2015
    Co-Authors: Kongari Saritha, Angireddy Rajesh, K Manjulatha, Oruganti H Setty, Suresh Yenugu
    Abstract:

    Herbal products derived from Hemidesmus indicus (L.) R. Br. ex Schult, Leucas aspera (Wild.), Plumbago zeylanica L., and Tridax procumbens (L.) R. Br. ex Schult. are widely used in traditional medicine. Though the extracts of these plants were found to be antimicrobial in nature and have the potential to be used in clinics, the mechanism of action of is not reported. The ethanolic extracts of Hemidesmus indicus (L.) R. Br. ex Schult, Hemidesmus indicus ethanolic extract (HIEE), Leucas aspera (Wild.), Leucas aspera ethanolic extract (LAEE), Plumbago zeylanica L., Plumbago zeylanica ethanolic extract (PZEE), and Tridax procumbens (L.) R. Br. ex Schult, Tridax procumbens ethanolic extract (TPEE) were tested for their antibacterial activity against E. coli. Antibacterial activity was analyzed by CFU assay and the effect on the bacterial membrane by fluorescence activated cell sorting and scanning electron microscopy. LAEE, PZEE, and HIEE displayed potent bacterial killing activity in a time and concentration dependent manner. TPEE did not display appreciable antibacterial activity. The antibacterial action involved disruption of membrane potential, inner membrane permeabilization, blebbing and leakage of cellular contents. Our results contribute to the understanding of the antibacterial mechanism of alcoholic extracts of the medicinal plants used in this study.