The Experts below are selected from a list of 306 Experts worldwide ranked by ideXlab platform
Kazumori Funatsu - One of the best experts on this subject based on the ideXlab platform.
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efficacy of a polyurethane foam spheroid artificial liver by using human Hepatoblastoma cell line Hep G2
Cell Transplantation, 2003Co-Authors: Junji Fukuda, K Okamura, Kohji Nakazawa, Hiroyuki Ijima, Y Yamashita, Mitsuo Shimada, Ken Shirabe, Eiji Tsujita, K Sugimachi, Kazumori FunatsuAbstract:We investigated the availability of human Hepatoblastoma cell line (Hep G2), compared with human primary Hepatocytes (HH) and porcine primary Hepatocytes (PH), as a cell source for the hybrid artificial liver support system (HALSS) by using polyurethane foam (PUF). All three kinds of Hepatocytes spontaneously formed spherical multicellular aggregates (spheroids) of 100–200 μm diameter in the pores of PUF within 3 days of culture. In a PUF stationary culture, Hep G2 spheroids recovered the ammonia removal activity that was lost in monolayer culture, although the removal for each unit cell number was about one tenth that of HH spheroids and about one eighth of PH spheroids. The synthesis activities of albumin and fibrinogen of each unit cell number of Hep G2 were also upregulated by PUF spheroid culture, and were about twice as high as in monolayer culture. The albumin secretion activity of Hep G2 spheroids was almost the same as that of PH spheroids. HH scarcely secreted these proteins in this experiment, ...
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Efficacy of a polyurethane foam/spheroid artificial liver by using human Hepatoblastoma cell line (Hep G2)
Cell Transplantation, 2003Co-Authors: Junji Fukuda, K Okamura, Kohji Nakazawa, Hiroyuki Ijima, Y Yamashita, Mitsuo Shimada, Ken Shirabe, Eiji Tsujita, Sugimachi K, Kazumori FunatsuAbstract:We investigated the availability of human Hepatoblastoma cell line (Hep G2), compared with human primary Hepatocytes (HH) and porcine primary Hepatocytes (PH), as a cell source for the hybrid artificial liver support system (HALSS) by using polyurethane foam (PUF). All three kinds of Hepatocytes spontaneously formed spherical multicellular aggregates (spheroids) of 100–200 μm diameter in the pores of PUF within 3 days of culture. In a PUF stationary culture, Hep G2 spheroids recovered the ammonia removal activity that was lost in monolayer culture, although the removal for each unit cell number was about one tenth that of HH spheroids and about one eighth of PH spheroids. The synthesis activities of albumin and fibrinogen of each unit cell number of Hep G2 were also upregulated by PUF spheroid culture, and were about twice as high as in monolayer culture. The albumin secretion activity of Hep G2 spheroids was almost the same as that of PH spheroids. HH scarcely secreted these proteins in this experiment, ...
Junji Fukuda - One of the best experts on this subject based on the ideXlab platform.
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efficacy of a polyurethane foam spheroid artificial liver by using human Hepatoblastoma cell line Hep G2
Cell Transplantation, 2003Co-Authors: Junji Fukuda, K Okamura, Kohji Nakazawa, Hiroyuki Ijima, Y Yamashita, Mitsuo Shimada, Ken Shirabe, Eiji Tsujita, K Sugimachi, Kazumori FunatsuAbstract:We investigated the availability of human Hepatoblastoma cell line (Hep G2), compared with human primary Hepatocytes (HH) and porcine primary Hepatocytes (PH), as a cell source for the hybrid artificial liver support system (HALSS) by using polyurethane foam (PUF). All three kinds of Hepatocytes spontaneously formed spherical multicellular aggregates (spheroids) of 100–200 μm diameter in the pores of PUF within 3 days of culture. In a PUF stationary culture, Hep G2 spheroids recovered the ammonia removal activity that was lost in monolayer culture, although the removal for each unit cell number was about one tenth that of HH spheroids and about one eighth of PH spheroids. The synthesis activities of albumin and fibrinogen of each unit cell number of Hep G2 were also upregulated by PUF spheroid culture, and were about twice as high as in monolayer culture. The albumin secretion activity of Hep G2 spheroids was almost the same as that of PH spheroids. HH scarcely secreted these proteins in this experiment, ...
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Efficacy of a polyurethane foam/spheroid artificial liver by using human Hepatoblastoma cell line (Hep G2)
Cell Transplantation, 2003Co-Authors: Junji Fukuda, K Okamura, Kohji Nakazawa, Hiroyuki Ijima, Y Yamashita, Mitsuo Shimada, Ken Shirabe, Eiji Tsujita, Sugimachi K, Kazumori FunatsuAbstract:We investigated the availability of human Hepatoblastoma cell line (Hep G2), compared with human primary Hepatocytes (HH) and porcine primary Hepatocytes (PH), as a cell source for the hybrid artificial liver support system (HALSS) by using polyurethane foam (PUF). All three kinds of Hepatocytes spontaneously formed spherical multicellular aggregates (spheroids) of 100–200 μm diameter in the pores of PUF within 3 days of culture. In a PUF stationary culture, Hep G2 spheroids recovered the ammonia removal activity that was lost in monolayer culture, although the removal for each unit cell number was about one tenth that of HH spheroids and about one eighth of PH spheroids. The synthesis activities of albumin and fibrinogen of each unit cell number of Hep G2 were also upregulated by PUF spheroid culture, and were about twice as high as in monolayer culture. The albumin secretion activity of Hep G2 spheroids was almost the same as that of PH spheroids. HH scarcely secreted these proteins in this experiment, ...
Haiyan Yang - One of the best experts on this subject based on the ideXlab platform.
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benzo a pyrene promotes Hep G2 cell migration and invasion by upregulating phosphorylated extracellular signal regulated kinase expression
Oncology Letters, 2018Co-Authors: Yadong Wang, Li Li, Haiyu Wang, Ding Zhang, Haiyan YangAbstract:Benzo(a)pyrene (BaP), a carcinogenic component of cigarette smoke, has been reported to activate extracellular signal-regulated kinase (ERK) in cancer cells. Furthermore, activated ERK is associated with liver cancer cell invasion and metastasis. Therefore, the aim of the present study was to investigate the potential role of phosphorylated (p)-ERK in BaP-induced Hep-G2 cell migration and invasion. An MTT assay was used to determine the effects of BaP treatment on Hep-G2 cell proliferation. Wound-healing and Transwell invasion assays were employed to assess the migration and invasion abilities of Hep-G2 cells. Western blot analysis was applied to detect the expression of proteins. The results of the present study demonstrated that BaP treatment was able to increase the level of p-ERK protein expression in Hep-G2 cells. BaP treatment promoted Hep-G2 cell migration and invasion. The ERK inhibitor, U0126, was able to block the migration and invasion abilities of Hep-G2 cells induced by BaP. The results of the present study demonstrated that BaP treatment promoted the migration and invasion of Hep-G2 cells by upregulating p-ERK expression.
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Benzo(a)pyrene promotes Hep‑G2 cell migration and invasion by upregulating phosphorylated extracellular signal‑regulated kinase expression
Oncology Letters, 2018Co-Authors: Yadong Wang, Li Li, Haiyu Wang, Ding Zhang, Haiyan YangAbstract:Benzo(a)pyrene (BaP), a carcinogenic component of cigarette smoke, has been reported to activate extracellular signal-regulated kinase (ERK) in cancer cells. Furthermore, activated ERK is associated with liver cancer cell invasion and metastasis. Therefore, the aim of the present study was to investigate the potential role of phosphorylated (p)-ERK in BaP-induced Hep-G2 cell migration and invasion. An MTT assay was used to determine the effects of BaP treatment on Hep-G2 cell proliferation. Wound-healing and Transwell invasion assays were employed to assess the migration and invasion abilities of Hep-G2 cells. Western blot analysis was applied to detect the expression of proteins. The results of the present study demonstrated that BaP treatment was able to increase the level of p-ERK protein expression in Hep-G2 cells. BaP treatment promoted Hep-G2 cell migration and invasion. The ERK inhibitor, U0126, was able to block the migration and invasion abilities of Hep-G2 cells induced by BaP. The results of the present study demonstrated that BaP treatment promoted the migration and invasion of Hep-G2 cells by upregulating p-ERK expression.
Hiroyuki Ijima - One of the best experts on this subject based on the ideXlab platform.
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efficacy of a polyurethane foam spheroid artificial liver by using human Hepatoblastoma cell line Hep G2
Cell Transplantation, 2003Co-Authors: Junji Fukuda, K Okamura, Kohji Nakazawa, Hiroyuki Ijima, Y Yamashita, Mitsuo Shimada, Ken Shirabe, Eiji Tsujita, K Sugimachi, Kazumori FunatsuAbstract:We investigated the availability of human Hepatoblastoma cell line (Hep G2), compared with human primary Hepatocytes (HH) and porcine primary Hepatocytes (PH), as a cell source for the hybrid artificial liver support system (HALSS) by using polyurethane foam (PUF). All three kinds of Hepatocytes spontaneously formed spherical multicellular aggregates (spheroids) of 100–200 μm diameter in the pores of PUF within 3 days of culture. In a PUF stationary culture, Hep G2 spheroids recovered the ammonia removal activity that was lost in monolayer culture, although the removal for each unit cell number was about one tenth that of HH spheroids and about one eighth of PH spheroids. The synthesis activities of albumin and fibrinogen of each unit cell number of Hep G2 were also upregulated by PUF spheroid culture, and were about twice as high as in monolayer culture. The albumin secretion activity of Hep G2 spheroids was almost the same as that of PH spheroids. HH scarcely secreted these proteins in this experiment, ...
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Efficacy of a polyurethane foam/spheroid artificial liver by using human Hepatoblastoma cell line (Hep G2)
Cell Transplantation, 2003Co-Authors: Junji Fukuda, K Okamura, Kohji Nakazawa, Hiroyuki Ijima, Y Yamashita, Mitsuo Shimada, Ken Shirabe, Eiji Tsujita, Sugimachi K, Kazumori FunatsuAbstract:We investigated the availability of human Hepatoblastoma cell line (Hep G2), compared with human primary Hepatocytes (HH) and porcine primary Hepatocytes (PH), as a cell source for the hybrid artificial liver support system (HALSS) by using polyurethane foam (PUF). All three kinds of Hepatocytes spontaneously formed spherical multicellular aggregates (spheroids) of 100–200 μm diameter in the pores of PUF within 3 days of culture. In a PUF stationary culture, Hep G2 spheroids recovered the ammonia removal activity that was lost in monolayer culture, although the removal for each unit cell number was about one tenth that of HH spheroids and about one eighth of PH spheroids. The synthesis activities of albumin and fibrinogen of each unit cell number of Hep G2 were also upregulated by PUF spheroid culture, and were about twice as high as in monolayer culture. The albumin secretion activity of Hep G2 spheroids was almost the same as that of PH spheroids. HH scarcely secreted these proteins in this experiment, ...
K Okamura - One of the best experts on this subject based on the ideXlab platform.
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efficacy of a polyurethane foam spheroid artificial liver by using human Hepatoblastoma cell line Hep G2
Cell Transplantation, 2003Co-Authors: Junji Fukuda, K Okamura, Kohji Nakazawa, Hiroyuki Ijima, Y Yamashita, Mitsuo Shimada, Ken Shirabe, Eiji Tsujita, K Sugimachi, Kazumori FunatsuAbstract:We investigated the availability of human Hepatoblastoma cell line (Hep G2), compared with human primary Hepatocytes (HH) and porcine primary Hepatocytes (PH), as a cell source for the hybrid artificial liver support system (HALSS) by using polyurethane foam (PUF). All three kinds of Hepatocytes spontaneously formed spherical multicellular aggregates (spheroids) of 100–200 μm diameter in the pores of PUF within 3 days of culture. In a PUF stationary culture, Hep G2 spheroids recovered the ammonia removal activity that was lost in monolayer culture, although the removal for each unit cell number was about one tenth that of HH spheroids and about one eighth of PH spheroids. The synthesis activities of albumin and fibrinogen of each unit cell number of Hep G2 were also upregulated by PUF spheroid culture, and were about twice as high as in monolayer culture. The albumin secretion activity of Hep G2 spheroids was almost the same as that of PH spheroids. HH scarcely secreted these proteins in this experiment, ...
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Efficacy of a polyurethane foam/spheroid artificial liver by using human Hepatoblastoma cell line (Hep G2)
Cell Transplantation, 2003Co-Authors: Junji Fukuda, K Okamura, Kohji Nakazawa, Hiroyuki Ijima, Y Yamashita, Mitsuo Shimada, Ken Shirabe, Eiji Tsujita, Sugimachi K, Kazumori FunatsuAbstract:We investigated the availability of human Hepatoblastoma cell line (Hep G2), compared with human primary Hepatocytes (HH) and porcine primary Hepatocytes (PH), as a cell source for the hybrid artificial liver support system (HALSS) by using polyurethane foam (PUF). All three kinds of Hepatocytes spontaneously formed spherical multicellular aggregates (spheroids) of 100–200 μm diameter in the pores of PUF within 3 days of culture. In a PUF stationary culture, Hep G2 spheroids recovered the ammonia removal activity that was lost in monolayer culture, although the removal for each unit cell number was about one tenth that of HH spheroids and about one eighth of PH spheroids. The synthesis activities of albumin and fibrinogen of each unit cell number of Hep G2 were also upregulated by PUF spheroid culture, and were about twice as high as in monolayer culture. The albumin secretion activity of Hep G2 spheroids was almost the same as that of PH spheroids. HH scarcely secreted these proteins in this experiment, ...