The Experts below are selected from a list of 318 Experts worldwide ranked by ideXlab platform
Beatrix Kapusinszky - One of the best experts on this subject based on the ideXlab platform.
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detection of a novel rna virus with hepatitis e virus like non structural genome organization in amphibian agile frog rana dalmatina tadpoles
Infection Genetics and Evolution, 2018Co-Authors: Gabor Reuter, Beatrix Kapusinszky, Akos Boros, Zoltan Toth, Peter PankovicsAbstract:Abstract In recent years, relatives (bastrovirus, hepelivirus) of Hepeviruses (family Hepeviridae) have been reported in a variety of vertebrate hosts. Preliminary studies indicated that inter-viral family recombination events at the junction of the genomes that encodes non-structural (ORF1) and structural protein (ORF2) were implicated in the genesis of Hepeviruses. Using viral metagenomics, next generation sequencing and RT-PCR techniques a genetically divergent Hepevirus-like RNA virus was identified and characterized from agile frog (Rana dalmatina) tadpoles living in aquatic environment in three natural ponds (Melymocsar, Lake Ilona and Lake Katlan) in the Pilis Mountains, in Hungary. The complete genome of the viral strain agile frog/RD6/2015/HUN (MH330682) is 7188 nt long including a 48-nt 5′ and a 122-nt 3′ non-coding region. Sequence analysis indicated that the agile frog/RD6/2015/HUN genome has potentially three non-overlapping ORFs. ORF1 (4740 nt/1579aa) has a Hepevirus-like non-structural genome organization and encodes several Hepevirus-like amino acid sequence motifs. The ORF2 is a potential capsid protein. The functions of the ORF3 were not predictable. The study virus was present in 18 (46%) of the 39 faecal specimen pools from agile frog tadpoles. The taxonomic position of this novel virus is presently unknown.
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divergent hepatitis e virus in birds of prey common kestrel falco tinnunculus and red footed falcon f vespertinus hungary
Infection Genetics and Evolution, 2016Co-Authors: Robert Matics, Beatrix KapusinszkyAbstract:Abstract Hepatitis E virus (HEV), family Hepeviridae, has raised considerable public health concerns because of its zoonotic potential; however, the animal to animal transmissions and the natural chain of Hepevirus infections in wildlife are less known. Using random amplification and next generation sequencing technology a novel HEV in birds of prey was serendipitously identified in Hungary. HEV RNA was detected in total of 2 (18%) of the 11 and 1 (14%) of the 7 faecal samples from common kestrels and red-footed falcons, respectively. High faecal viral load (2.03 × 108 genomic copies/ml) measured by qPCR. The complete genome of strain kestrel/MR22/2014/HUN (KU670940) HEV is 7033-nt long including a 35-nt 5'end and a 63-nt 3'end (excluding the poly(A)-tail). Sequence analyses indicated that the ORF1 (4920 nt/639 aa), ORF2 (1989 nt/662 aa) and ORF3 (360 nt/119aa) proteins of kestrel/MR22/2014/HUN shared the highest identity (58.1%, 66.8% and 28.5%) to the corresponding proteins of ferret, rat and human genotype 4 OrthoHepeviruses, respectively. Interestingly, the ORF3 protein is potentially initiated with leucine (L) using an alternate, non-AUG (UUG) start codon. This study reports the identification and complete genome characterization of a novel OrthoHepevirus species related to mammalian HEVs in birds of prey. It is important to recognize all potential hosts, reservoirs and spreaders in nature and to reconstruct the phylogenetic history of Hepeviruses.
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divergent hepatitis e virus in birds of prey common kestrel falco tinnunculus and red footed falcon f vespertinus hungary
Infection Genetics and Evolution, 2016Co-Authors: Gabor Reuter, Robert Matics, Beatrix Kapusinszky, Akos Boros, Peter PankovicsAbstract:Abstract Hepatitis E virus (HEV), family Hepeviridae, has raised considerable public health concerns because of its zoonotic potential; however, the animal to animal transmissions and the natural chain of Hepevirus infections in wildlife are less known. Using random amplification and next generation sequencing technology a novel HEV in birds of prey was serendipitously identified in Hungary. HEV RNA was detected in total of 2 (18%) of the 11 and 1 (14%) of the 7 faecal samples from common kestrels and red-footed falcons, respectively. High faecal viral load (2.03 × 108 genomic copies/ml) measured by qPCR. The complete genome of strain kestrel/MR22/2014/HUN (KU670940) HEV is 7033-nt long including a 35-nt 5'end and a 63-nt 3'end (excluding the poly(A)-tail). Sequence analyses indicated that the ORF1 (4920 nt/639 aa), ORF2 (1989 nt/662 aa) and ORF3 (360 nt/119aa) proteins of kestrel/MR22/2014/HUN shared the highest identity (58.1%, 66.8% and 28.5%) to the corresponding proteins of ferret, rat and human genotype 4 OrthoHepeviruses, respectively. Interestingly, the ORF3 protein is potentially initiated with leucine (L) using an alternate, non-AUG (UUG) start codon. This study reports the identification and complete genome characterization of a novel OrthoHepevirus species related to mammalian HEVs in birds of prey. It is important to recognize all potential hosts, reservoirs and spreaders in nature and to reconstruct the phylogenetic history of Hepeviruses.
Peter Pankovics - One of the best experts on this subject based on the ideXlab platform.
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genome characterization prevalence and tissue distribution of astrovirus Hepevirus and norovirus among wild and laboratory rats rattus norvegicus and mice mus musculus in hungary
Infection Genetics and Evolution, 2021Co-Authors: Peter Pankovics, Akos Boros, Zoltan Laszlo, Sandor Szekeres, Gabor Foldvari, Eda Altan, Eric Delwart, Gabor ReuterAbstract:Abstract Rodents including rats are reservoir of several pathogens capable of affecting human health. In this study, faecal and different organ specimens from free-living Norway rats (Rattus norvegicus) (N = 18) and faecal samples from laboratory rodents (rats N = 21 and mice N = 20) collected from different geographic areas in Hungary between 2017 and 2020 were investigated by viral metagenomics and conventional RT-PCR methods. The complete genome of three different RNA viruses, rat astrovirus, rat norovirus and rat Hepevirus were characterized and analysed in detail. Rat norovirus was detected in faecal (17.6%, 3/17) and kidney (7.1%, 1/14) samples; rat astrovirus in faecal (23.5%, 4/17) and spleen (13.3%, 2/15) samples, and rat Hepevirus in 43% to 67% the faecal, liver, kidney, lung, heart, muscle, brain and blood samples from Norway rats, respectively. Rat norovirus was also identifiable in 5% (1/21) of laboratory rats and rat astrovirus in 40% (8/20) of faecal samples from laboratory mice. Co-infections were found in 28% (5/18) wild Norway rats. The highest RNA viral load of astrovirus (1.81 × 108 copy/g) and norovirus (3.49 × 107 copy/g) were measured in faecal samples; while the highest RNA viral load of Hepevirus (1.16 × 109 copy/g) was found in liver samples of Norway rats, respectively. This study confirms the wide geographic distribution and high prevalence of astrovirus, norovirus and Hepevirus among wild rats in Hungary with confirmation of different organ involvement of as well as the detection of norovirus and astrovirus in laboratory rats and mice, respectively. This finding further strengthens the role of rodents in the spread of viral pathogens especially infecting human.
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detection of a novel rna virus with hepatitis e virus like non structural genome organization in amphibian agile frog rana dalmatina tadpoles
Infection Genetics and Evolution, 2018Co-Authors: Gabor Reuter, Beatrix Kapusinszky, Akos Boros, Zoltan Toth, Peter PankovicsAbstract:Abstract In recent years, relatives (bastrovirus, hepelivirus) of Hepeviruses (family Hepeviridae) have been reported in a variety of vertebrate hosts. Preliminary studies indicated that inter-viral family recombination events at the junction of the genomes that encodes non-structural (ORF1) and structural protein (ORF2) were implicated in the genesis of Hepeviruses. Using viral metagenomics, next generation sequencing and RT-PCR techniques a genetically divergent Hepevirus-like RNA virus was identified and characterized from agile frog (Rana dalmatina) tadpoles living in aquatic environment in three natural ponds (Melymocsar, Lake Ilona and Lake Katlan) in the Pilis Mountains, in Hungary. The complete genome of the viral strain agile frog/RD6/2015/HUN (MH330682) is 7188 nt long including a 48-nt 5′ and a 122-nt 3′ non-coding region. Sequence analysis indicated that the agile frog/RD6/2015/HUN genome has potentially three non-overlapping ORFs. ORF1 (4740 nt/1579aa) has a Hepevirus-like non-structural genome organization and encodes several Hepevirus-like amino acid sequence motifs. The ORF2 is a potential capsid protein. The functions of the ORF3 were not predictable. The study virus was present in 18 (46%) of the 39 faecal specimen pools from agile frog tadpoles. The taxonomic position of this novel virus is presently unknown.
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divergent hepatitis e virus in birds of prey common kestrel falco tinnunculus and red footed falcon f vespertinus hungary
Infection Genetics and Evolution, 2016Co-Authors: Gabor Reuter, Robert Matics, Beatrix Kapusinszky, Akos Boros, Peter PankovicsAbstract:Abstract Hepatitis E virus (HEV), family Hepeviridae, has raised considerable public health concerns because of its zoonotic potential; however, the animal to animal transmissions and the natural chain of Hepevirus infections in wildlife are less known. Using random amplification and next generation sequencing technology a novel HEV in birds of prey was serendipitously identified in Hungary. HEV RNA was detected in total of 2 (18%) of the 11 and 1 (14%) of the 7 faecal samples from common kestrels and red-footed falcons, respectively. High faecal viral load (2.03 × 108 genomic copies/ml) measured by qPCR. The complete genome of strain kestrel/MR22/2014/HUN (KU670940) HEV is 7033-nt long including a 35-nt 5'end and a 63-nt 3'end (excluding the poly(A)-tail). Sequence analyses indicated that the ORF1 (4920 nt/639 aa), ORF2 (1989 nt/662 aa) and ORF3 (360 nt/119aa) proteins of kestrel/MR22/2014/HUN shared the highest identity (58.1%, 66.8% and 28.5%) to the corresponding proteins of ferret, rat and human genotype 4 OrthoHepeviruses, respectively. Interestingly, the ORF3 protein is potentially initiated with leucine (L) using an alternate, non-AUG (UUG) start codon. This study reports the identification and complete genome characterization of a novel OrthoHepevirus species related to mammalian HEVs in birds of prey. It is important to recognize all potential hosts, reservoirs and spreaders in nature and to reconstruct the phylogenetic history of Hepeviruses.
Gabor Reuter - One of the best experts on this subject based on the ideXlab platform.
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genome characterization prevalence and tissue distribution of astrovirus Hepevirus and norovirus among wild and laboratory rats rattus norvegicus and mice mus musculus in hungary
Infection Genetics and Evolution, 2021Co-Authors: Peter Pankovics, Akos Boros, Zoltan Laszlo, Sandor Szekeres, Gabor Foldvari, Eda Altan, Eric Delwart, Gabor ReuterAbstract:Abstract Rodents including rats are reservoir of several pathogens capable of affecting human health. In this study, faecal and different organ specimens from free-living Norway rats (Rattus norvegicus) (N = 18) and faecal samples from laboratory rodents (rats N = 21 and mice N = 20) collected from different geographic areas in Hungary between 2017 and 2020 were investigated by viral metagenomics and conventional RT-PCR methods. The complete genome of three different RNA viruses, rat astrovirus, rat norovirus and rat Hepevirus were characterized and analysed in detail. Rat norovirus was detected in faecal (17.6%, 3/17) and kidney (7.1%, 1/14) samples; rat astrovirus in faecal (23.5%, 4/17) and spleen (13.3%, 2/15) samples, and rat Hepevirus in 43% to 67% the faecal, liver, kidney, lung, heart, muscle, brain and blood samples from Norway rats, respectively. Rat norovirus was also identifiable in 5% (1/21) of laboratory rats and rat astrovirus in 40% (8/20) of faecal samples from laboratory mice. Co-infections were found in 28% (5/18) wild Norway rats. The highest RNA viral load of astrovirus (1.81 × 108 copy/g) and norovirus (3.49 × 107 copy/g) were measured in faecal samples; while the highest RNA viral load of Hepevirus (1.16 × 109 copy/g) was found in liver samples of Norway rats, respectively. This study confirms the wide geographic distribution and high prevalence of astrovirus, norovirus and Hepevirus among wild rats in Hungary with confirmation of different organ involvement of as well as the detection of norovirus and astrovirus in laboratory rats and mice, respectively. This finding further strengthens the role of rodents in the spread of viral pathogens especially infecting human.
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detection of a novel rna virus with hepatitis e virus like non structural genome organization in amphibian agile frog rana dalmatina tadpoles
Infection Genetics and Evolution, 2018Co-Authors: Gabor Reuter, Beatrix Kapusinszky, Akos Boros, Zoltan Toth, Peter PankovicsAbstract:Abstract In recent years, relatives (bastrovirus, hepelivirus) of Hepeviruses (family Hepeviridae) have been reported in a variety of vertebrate hosts. Preliminary studies indicated that inter-viral family recombination events at the junction of the genomes that encodes non-structural (ORF1) and structural protein (ORF2) were implicated in the genesis of Hepeviruses. Using viral metagenomics, next generation sequencing and RT-PCR techniques a genetically divergent Hepevirus-like RNA virus was identified and characterized from agile frog (Rana dalmatina) tadpoles living in aquatic environment in three natural ponds (Melymocsar, Lake Ilona and Lake Katlan) in the Pilis Mountains, in Hungary. The complete genome of the viral strain agile frog/RD6/2015/HUN (MH330682) is 7188 nt long including a 48-nt 5′ and a 122-nt 3′ non-coding region. Sequence analysis indicated that the agile frog/RD6/2015/HUN genome has potentially three non-overlapping ORFs. ORF1 (4740 nt/1579aa) has a Hepevirus-like non-structural genome organization and encodes several Hepevirus-like amino acid sequence motifs. The ORF2 is a potential capsid protein. The functions of the ORF3 were not predictable. The study virus was present in 18 (46%) of the 39 faecal specimen pools from agile frog tadpoles. The taxonomic position of this novel virus is presently unknown.
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divergent hepatitis e virus in birds of prey common kestrel falco tinnunculus and red footed falcon f vespertinus hungary
Infection Genetics and Evolution, 2016Co-Authors: Gabor Reuter, Robert Matics, Beatrix Kapusinszky, Akos Boros, Peter PankovicsAbstract:Abstract Hepatitis E virus (HEV), family Hepeviridae, has raised considerable public health concerns because of its zoonotic potential; however, the animal to animal transmissions and the natural chain of Hepevirus infections in wildlife are less known. Using random amplification and next generation sequencing technology a novel HEV in birds of prey was serendipitously identified in Hungary. HEV RNA was detected in total of 2 (18%) of the 11 and 1 (14%) of the 7 faecal samples from common kestrels and red-footed falcons, respectively. High faecal viral load (2.03 × 108 genomic copies/ml) measured by qPCR. The complete genome of strain kestrel/MR22/2014/HUN (KU670940) HEV is 7033-nt long including a 35-nt 5'end and a 63-nt 3'end (excluding the poly(A)-tail). Sequence analyses indicated that the ORF1 (4920 nt/639 aa), ORF2 (1989 nt/662 aa) and ORF3 (360 nt/119aa) proteins of kestrel/MR22/2014/HUN shared the highest identity (58.1%, 66.8% and 28.5%) to the corresponding proteins of ferret, rat and human genotype 4 OrthoHepeviruses, respectively. Interestingly, the ORF3 protein is potentially initiated with leucine (L) using an alternate, non-AUG (UUG) start codon. This study reports the identification and complete genome characterization of a novel OrthoHepevirus species related to mammalian HEVs in birds of prey. It is important to recognize all potential hosts, reservoirs and spreaders in nature and to reconstruct the phylogenetic history of Hepeviruses.
Robert Matics - One of the best experts on this subject based on the ideXlab platform.
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divergent hepatitis e virus in birds of prey common kestrel falco tinnunculus and red footed falcon f vespertinus hungary
Infection Genetics and Evolution, 2016Co-Authors: Robert Matics, Beatrix KapusinszkyAbstract:Abstract Hepatitis E virus (HEV), family Hepeviridae, has raised considerable public health concerns because of its zoonotic potential; however, the animal to animal transmissions and the natural chain of Hepevirus infections in wildlife are less known. Using random amplification and next generation sequencing technology a novel HEV in birds of prey was serendipitously identified in Hungary. HEV RNA was detected in total of 2 (18%) of the 11 and 1 (14%) of the 7 faecal samples from common kestrels and red-footed falcons, respectively. High faecal viral load (2.03 × 108 genomic copies/ml) measured by qPCR. The complete genome of strain kestrel/MR22/2014/HUN (KU670940) HEV is 7033-nt long including a 35-nt 5'end and a 63-nt 3'end (excluding the poly(A)-tail). Sequence analyses indicated that the ORF1 (4920 nt/639 aa), ORF2 (1989 nt/662 aa) and ORF3 (360 nt/119aa) proteins of kestrel/MR22/2014/HUN shared the highest identity (58.1%, 66.8% and 28.5%) to the corresponding proteins of ferret, rat and human genotype 4 OrthoHepeviruses, respectively. Interestingly, the ORF3 protein is potentially initiated with leucine (L) using an alternate, non-AUG (UUG) start codon. This study reports the identification and complete genome characterization of a novel OrthoHepevirus species related to mammalian HEVs in birds of prey. It is important to recognize all potential hosts, reservoirs and spreaders in nature and to reconstruct the phylogenetic history of Hepeviruses.
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divergent hepatitis e virus in birds of prey common kestrel falco tinnunculus and red footed falcon f vespertinus hungary
Infection Genetics and Evolution, 2016Co-Authors: Gabor Reuter, Robert Matics, Beatrix Kapusinszky, Akos Boros, Peter PankovicsAbstract:Abstract Hepatitis E virus (HEV), family Hepeviridae, has raised considerable public health concerns because of its zoonotic potential; however, the animal to animal transmissions and the natural chain of Hepevirus infections in wildlife are less known. Using random amplification and next generation sequencing technology a novel HEV in birds of prey was serendipitously identified in Hungary. HEV RNA was detected in total of 2 (18%) of the 11 and 1 (14%) of the 7 faecal samples from common kestrels and red-footed falcons, respectively. High faecal viral load (2.03 × 108 genomic copies/ml) measured by qPCR. The complete genome of strain kestrel/MR22/2014/HUN (KU670940) HEV is 7033-nt long including a 35-nt 5'end and a 63-nt 3'end (excluding the poly(A)-tail). Sequence analyses indicated that the ORF1 (4920 nt/639 aa), ORF2 (1989 nt/662 aa) and ORF3 (360 nt/119aa) proteins of kestrel/MR22/2014/HUN shared the highest identity (58.1%, 66.8% and 28.5%) to the corresponding proteins of ferret, rat and human genotype 4 OrthoHepeviruses, respectively. Interestingly, the ORF3 protein is potentially initiated with leucine (L) using an alternate, non-AUG (UUG) start codon. This study reports the identification and complete genome characterization of a novel OrthoHepevirus species related to mammalian HEVs in birds of prey. It is important to recognize all potential hosts, reservoirs and spreaders in nature and to reconstruct the phylogenetic history of Hepeviruses.
Akos Boros - One of the best experts on this subject based on the ideXlab platform.
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genome characterization prevalence and tissue distribution of astrovirus Hepevirus and norovirus among wild and laboratory rats rattus norvegicus and mice mus musculus in hungary
Infection Genetics and Evolution, 2021Co-Authors: Peter Pankovics, Akos Boros, Zoltan Laszlo, Sandor Szekeres, Gabor Foldvari, Eda Altan, Eric Delwart, Gabor ReuterAbstract:Abstract Rodents including rats are reservoir of several pathogens capable of affecting human health. In this study, faecal and different organ specimens from free-living Norway rats (Rattus norvegicus) (N = 18) and faecal samples from laboratory rodents (rats N = 21 and mice N = 20) collected from different geographic areas in Hungary between 2017 and 2020 were investigated by viral metagenomics and conventional RT-PCR methods. The complete genome of three different RNA viruses, rat astrovirus, rat norovirus and rat Hepevirus were characterized and analysed in detail. Rat norovirus was detected in faecal (17.6%, 3/17) and kidney (7.1%, 1/14) samples; rat astrovirus in faecal (23.5%, 4/17) and spleen (13.3%, 2/15) samples, and rat Hepevirus in 43% to 67% the faecal, liver, kidney, lung, heart, muscle, brain and blood samples from Norway rats, respectively. Rat norovirus was also identifiable in 5% (1/21) of laboratory rats and rat astrovirus in 40% (8/20) of faecal samples from laboratory mice. Co-infections were found in 28% (5/18) wild Norway rats. The highest RNA viral load of astrovirus (1.81 × 108 copy/g) and norovirus (3.49 × 107 copy/g) were measured in faecal samples; while the highest RNA viral load of Hepevirus (1.16 × 109 copy/g) was found in liver samples of Norway rats, respectively. This study confirms the wide geographic distribution and high prevalence of astrovirus, norovirus and Hepevirus among wild rats in Hungary with confirmation of different organ involvement of as well as the detection of norovirus and astrovirus in laboratory rats and mice, respectively. This finding further strengthens the role of rodents in the spread of viral pathogens especially infecting human.
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detection of a novel rna virus with hepatitis e virus like non structural genome organization in amphibian agile frog rana dalmatina tadpoles
Infection Genetics and Evolution, 2018Co-Authors: Gabor Reuter, Beatrix Kapusinszky, Akos Boros, Zoltan Toth, Peter PankovicsAbstract:Abstract In recent years, relatives (bastrovirus, hepelivirus) of Hepeviruses (family Hepeviridae) have been reported in a variety of vertebrate hosts. Preliminary studies indicated that inter-viral family recombination events at the junction of the genomes that encodes non-structural (ORF1) and structural protein (ORF2) were implicated in the genesis of Hepeviruses. Using viral metagenomics, next generation sequencing and RT-PCR techniques a genetically divergent Hepevirus-like RNA virus was identified and characterized from agile frog (Rana dalmatina) tadpoles living in aquatic environment in three natural ponds (Melymocsar, Lake Ilona and Lake Katlan) in the Pilis Mountains, in Hungary. The complete genome of the viral strain agile frog/RD6/2015/HUN (MH330682) is 7188 nt long including a 48-nt 5′ and a 122-nt 3′ non-coding region. Sequence analysis indicated that the agile frog/RD6/2015/HUN genome has potentially three non-overlapping ORFs. ORF1 (4740 nt/1579aa) has a Hepevirus-like non-structural genome organization and encodes several Hepevirus-like amino acid sequence motifs. The ORF2 is a potential capsid protein. The functions of the ORF3 were not predictable. The study virus was present in 18 (46%) of the 39 faecal specimen pools from agile frog tadpoles. The taxonomic position of this novel virus is presently unknown.
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divergent hepatitis e virus in birds of prey common kestrel falco tinnunculus and red footed falcon f vespertinus hungary
Infection Genetics and Evolution, 2016Co-Authors: Gabor Reuter, Robert Matics, Beatrix Kapusinszky, Akos Boros, Peter PankovicsAbstract:Abstract Hepatitis E virus (HEV), family Hepeviridae, has raised considerable public health concerns because of its zoonotic potential; however, the animal to animal transmissions and the natural chain of Hepevirus infections in wildlife are less known. Using random amplification and next generation sequencing technology a novel HEV in birds of prey was serendipitously identified in Hungary. HEV RNA was detected in total of 2 (18%) of the 11 and 1 (14%) of the 7 faecal samples from common kestrels and red-footed falcons, respectively. High faecal viral load (2.03 × 108 genomic copies/ml) measured by qPCR. The complete genome of strain kestrel/MR22/2014/HUN (KU670940) HEV is 7033-nt long including a 35-nt 5'end and a 63-nt 3'end (excluding the poly(A)-tail). Sequence analyses indicated that the ORF1 (4920 nt/639 aa), ORF2 (1989 nt/662 aa) and ORF3 (360 nt/119aa) proteins of kestrel/MR22/2014/HUN shared the highest identity (58.1%, 66.8% and 28.5%) to the corresponding proteins of ferret, rat and human genotype 4 OrthoHepeviruses, respectively. Interestingly, the ORF3 protein is potentially initiated with leucine (L) using an alternate, non-AUG (UUG) start codon. This study reports the identification and complete genome characterization of a novel OrthoHepevirus species related to mammalian HEVs in birds of prey. It is important to recognize all potential hosts, reservoirs and spreaders in nature and to reconstruct the phylogenetic history of Hepeviruses.