The Experts below are selected from a list of 45 Experts worldwide ranked by ideXlab platform
Anna Członkowska - One of the best experts on this subject based on the ideXlab platform.
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Phenotyping analysis of peripheral blood leukocytes in patients with multiple sclerosis
European journal of neurology, 1999Co-Authors: Aleksandra Paź, Urszula Fiszer, J. Zaborski, Janina Korlak, Andrzej Członkowski, Anna CzłonkowskaAbstract:Multiple sclerosis (MS) is a central nervous disease thought to be elicited by an autoimmune process. Many studies in recent years have concentrated on finding the alterations in the peripheral blood immune profile in MS patients that would reflect disease activity. In the present study, we investigated surface Antigen expression on lymphocytes and granulocytes from MS patients and control subjects. We have studied 29 patients suffering from relapsing-remitting or relapsing-progressive forms of MS. The disease was diagnosed in all patients at least 12 months before inclusion into the study. All patients had no attack at the study entry date or within a previous month. The control group included 29 agematched subjects. Phenotyping of peripheral blood leukocytes was carried out with different fluorescence-conjugated murine monoclonal antibodies. The analysis was performed with three-color flow cytometry. The following Antigens were determined [cluster of definition (CD)]: leukocyte common Antigen (LCA) (B220, T 200, Ly-5), CD45; LPS-R (lipopolysaccharide receptor), CD14; found on all T cells, CD3; LFA-2 (lymphocyte function associated Antigen, T 11), CD2; coreceptor for MHC class II molecules, found on helper T cells, CD4; coreceptor for MHC class I molecules, found on suppressor/cytotoxic T cells, CD8; B4, found on all human B cells, CD19; NCAM (neural cell adhesion molecule), CD56; integrin β2 subunit, associated with CD11a (CD11a/CD18, LFA-1 αLβ2) and CD11b (CD11b/CD18, Mac-1,CR3, αMβ2), CD18; αL, α subunit of integrin LFA-1 (αLβ2, CD11a/CD18), CD11a; αM, α subunit of integrin Mac-1 (CR3, αMβ2, CD11b/CD18), CD11b; ICAM-1 (intercellular adhesion molecule), CD54; H-CAM, Hermes Antigen, Pgp-1, CD44; AIM (activation inducer molecule), early activation Antigen, CD69; T-cell receptor γδ. TCR γδ. In the MS group, we have found a significant increased expression of CD54 and CD44 Antigens on lymphocytes, and higher percentage CD54+and CD11a+CD54+ lymphocytes out of all lymphocytes compared with the control group. We have also found a significant increased expression of CD11a, CD18 and CD54 Antigens on lymphocytes, and higher percentage CD54+and CD11a+CD54+ lymphocytes out of all granulocytes in MS patients compared with control. Higher levels of expression of the adhesion molecules may reflect the activation state of leukocytes in MS patients.
Derek N.j. Hart - One of the best experts on this subject based on the ideXlab platform.
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Adhesion molecules on human tonsil dendritic cells.
Transplantation, 1992Co-Authors: Timothy C. R. Prickett, Judith L. Mckenzie, Derek N.j. HartAbstract:Dendritic cells are specialist Antigen-presenting cells that have a unique ability to stimulate a primary T cell response. Activation of T cells by DC depends on the formation of cell clusters creating DC-T cell membrane contact that probably involves adhesion molecules. Monoclonal antibodies were used to study adhesion molecules on DC, including members of the integrin and immunoglobulin supergene families. DC expressed LFA-1, ICAM-1, LFA-3, and the Hermes Antigen, but no other integrin or immunoglobulin supergene family adhesion molecules were detected using a sensitive immunoperoxidase staining technique. Monoclonal antibodies to LFA-1 alpha and LFA-1 beta inhibited DC-stimulated allogeneic T cell (MLR) responses by 75 +/- 12% and 74 +/- 8%, respectively, as did the anti-LFA-3 (56 +/- 3% inhibition) and anti-LFA-2 (60 +/- 5% inhibition) antibodies. Three different anti-ICAM-1 antibodies inhibited only to a limited degree (mean range 8-24%). The inhibitory effect of the LFA-1 and LFA-3 antibodies was maximal if added early to the MLR. The inhibitory effect of the different antibodies was associated with variable decreases in DC-T cell cluster stability. The simultaneous addition of monoclonal antibodies to MLRs and preincubation washing experiments established that DC have at least 3 independent adhesion ligand interactions (LFA-1-ICAM-1, ICAM-1-LFA-1, and LFA-3-CD2) with T cells. It seems likely that the additional ligand for LFA-1, ICAM-2, is expressed on DC and contributes significantly to DC-T cell adherence and T cell activation. The membrane mobility of these molecules may also be important in the DC-T cell activation process.
Roberto Conte - One of the best experts on this subject based on the ideXlab platform.
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Cell adhesion molecules CD11a and CD18 in blood monocytes in old age and the consequences for immunological dysfunction. Preliminary results
Gerontology, 1995Co-Authors: Mariella Chiricolo, Maria Morini, Rita Mancini, E. Beltrandi, Daniela Belletti, Roberto ConteAbstract:Adhesion molecules, such as leukocyte-function-associated Antigen (LFA-1 or CD11a/CD18), intercellular adhesion molecule 1 (ICAM-1 or CD54) and Hermes Antigen (HCAM or CD44), have important roles in many adhesive interactions involving cells of the immune system. Since it has been shown that many immunological alterations were present in aged subjects, we studied the expression and density of these molecules on peripheral blood lymphocytes and monocytes from healthy old subjects. A decrease in monocyte subpopulations bearing CD11a/CD18 and an increase in CD11a/CD18 and and CD44 Antigen density on lymphocytes and on monocytes, respectively, were observed. These changes might be an event in the mechanism leading to the decreased lymphocyte proliferative response in vitro and to other immunological dysfunctions reported in old subjects.
Aleksandra Paź - One of the best experts on this subject based on the ideXlab platform.
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Phenotyping analysis of peripheral blood leukocytes in patients with multiple sclerosis
European journal of neurology, 1999Co-Authors: Aleksandra Paź, Urszula Fiszer, J. Zaborski, Janina Korlak, Andrzej Członkowski, Anna CzłonkowskaAbstract:Multiple sclerosis (MS) is a central nervous disease thought to be elicited by an autoimmune process. Many studies in recent years have concentrated on finding the alterations in the peripheral blood immune profile in MS patients that would reflect disease activity. In the present study, we investigated surface Antigen expression on lymphocytes and granulocytes from MS patients and control subjects. We have studied 29 patients suffering from relapsing-remitting or relapsing-progressive forms of MS. The disease was diagnosed in all patients at least 12 months before inclusion into the study. All patients had no attack at the study entry date or within a previous month. The control group included 29 agematched subjects. Phenotyping of peripheral blood leukocytes was carried out with different fluorescence-conjugated murine monoclonal antibodies. The analysis was performed with three-color flow cytometry. The following Antigens were determined [cluster of definition (CD)]: leukocyte common Antigen (LCA) (B220, T 200, Ly-5), CD45; LPS-R (lipopolysaccharide receptor), CD14; found on all T cells, CD3; LFA-2 (lymphocyte function associated Antigen, T 11), CD2; coreceptor for MHC class II molecules, found on helper T cells, CD4; coreceptor for MHC class I molecules, found on suppressor/cytotoxic T cells, CD8; B4, found on all human B cells, CD19; NCAM (neural cell adhesion molecule), CD56; integrin β2 subunit, associated with CD11a (CD11a/CD18, LFA-1 αLβ2) and CD11b (CD11b/CD18, Mac-1,CR3, αMβ2), CD18; αL, α subunit of integrin LFA-1 (αLβ2, CD11a/CD18), CD11a; αM, α subunit of integrin Mac-1 (CR3, αMβ2, CD11b/CD18), CD11b; ICAM-1 (intercellular adhesion molecule), CD54; H-CAM, Hermes Antigen, Pgp-1, CD44; AIM (activation inducer molecule), early activation Antigen, CD69; T-cell receptor γδ. TCR γδ. In the MS group, we have found a significant increased expression of CD54 and CD44 Antigens on lymphocytes, and higher percentage CD54+and CD11a+CD54+ lymphocytes out of all lymphocytes compared with the control group. We have also found a significant increased expression of CD11a, CD18 and CD54 Antigens on lymphocytes, and higher percentage CD54+and CD11a+CD54+ lymphocytes out of all granulocytes in MS patients compared with control. Higher levels of expression of the adhesion molecules may reflect the activation state of leukocytes in MS patients.
Timothy C. R. Prickett - One of the best experts on this subject based on the ideXlab platform.
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Adhesion molecules on human tonsil dendritic cells.
Transplantation, 1992Co-Authors: Timothy C. R. Prickett, Judith L. Mckenzie, Derek N.j. HartAbstract:Dendritic cells are specialist Antigen-presenting cells that have a unique ability to stimulate a primary T cell response. Activation of T cells by DC depends on the formation of cell clusters creating DC-T cell membrane contact that probably involves adhesion molecules. Monoclonal antibodies were used to study adhesion molecules on DC, including members of the integrin and immunoglobulin supergene families. DC expressed LFA-1, ICAM-1, LFA-3, and the Hermes Antigen, but no other integrin or immunoglobulin supergene family adhesion molecules were detected using a sensitive immunoperoxidase staining technique. Monoclonal antibodies to LFA-1 alpha and LFA-1 beta inhibited DC-stimulated allogeneic T cell (MLR) responses by 75 +/- 12% and 74 +/- 8%, respectively, as did the anti-LFA-3 (56 +/- 3% inhibition) and anti-LFA-2 (60 +/- 5% inhibition) antibodies. Three different anti-ICAM-1 antibodies inhibited only to a limited degree (mean range 8-24%). The inhibitory effect of the LFA-1 and LFA-3 antibodies was maximal if added early to the MLR. The inhibitory effect of the different antibodies was associated with variable decreases in DC-T cell cluster stability. The simultaneous addition of monoclonal antibodies to MLRs and preincubation washing experiments established that DC have at least 3 independent adhesion ligand interactions (LFA-1-ICAM-1, ICAM-1-LFA-1, and LFA-3-CD2) with T cells. It seems likely that the additional ligand for LFA-1, ICAM-2, is expressed on DC and contributes significantly to DC-T cell adherence and T cell activation. The membrane mobility of these molecules may also be important in the DC-T cell activation process.