The Experts below are selected from a list of 258 Experts worldwide ranked by ideXlab platform
Xingxiao Zhang - One of the best experts on this subject based on the ideXlab platform.
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evidence of two genetically different lymphotropic Herpesviruses present among red deer sambar and milu herds in china
Journal of Veterinary Science, 2018Co-Authors: Hongwei Zhu, Huitao Liu, Jianlong Zhang, Linlin Jiang, Guozhong Chen, Zhibin Feng, Tao Feng, Xingxiao ZhangAbstract:Herpesvirus infections in Cervidae are a serious threat affecting some deer species worldwide. In our attempt to identify malignant catarrhal fever-associated Herpesviruses in deer herds, ten gammaherpesviral DNA fragments were identified in five species of deer in herds in China by using a pan-Herpesvirus polymerase chain reaction assay targeting viral DNA polymerase. Notably, in sambar (Rusa unicolor), a novel gamma-2 Herpesvirus was identified that showed a close relationship with fallow deer lymphotropic Herpesvirus (LHV), while the other fragments were phylogenetically grouped together with Elk-LHV. Determination of whether these viruses have any clinical implication in these deer species should be undertaken urgently.
Brigitte Biesinger - One of the best experts on this subject based on the ideXlab platform.
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Herpesvirus Ateles Tio Can Replace Herpesvirus Saimiri StpC and Tip Oncoproteins in Growth Transformation of Monkey and Human T Cells
Journal of virology, 2004Co-Authors: Jens-christian Albrecht, Bernhard Fleckenstein, Brigitte Biesinger, Ingrid Müller-fleckenstein, Doris Lengenfelder, Monika Schmidt, Armin EnsserAbstract:Herpesvirus saimiri group C strains are capable of transforming human and simian T-lymphocyte populations to permanent antigen-independent growth. Two viral oncoproteins, StpC and Tip, that are encoded by a single bicistronic mRNA, act in concert to mediate this phenotype. A closely related New World monkey Herpesvirus, Herpesvirus ateles, transcribes a single spliced mRNA at an equivalent genome locus. The encoded protein, Tio, has sequence homologies to both StpC and Tip. We inserted the tio sequence of Herpesvirus ateles strain 73 into a recombinant Herpesvirus saimiri C488 lacking its own stpC/tip oncogene. Simian as well as human T lymphocytes were growth transformed by the chimeric Tio-expressing viruses. Thus, a single Herpesvirus protein appears to be responsible for the oncogenic effects of Herpesvirus ateles.
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Herpesvirus Ateles Gene Product Tio Interacts with Nonreceptor Protein Tyrosine Kinases
Journal of virology, 1999Co-Authors: Jens-christian Albrecht, Bernhard Fleckenstein, Ute Friedrich, Christian Kardinal, Jadranka Koehn, Stephan M. Feller, Brigitte BiesingerAbstract:Herpesvirus ateles is a gamma-2-Herpesvirus which naturally infects spider monkeys (Ateles spp.) and causes malignant lymphoproliferative disorders in various other New World primates. The genomic sequence of Herpesvirus ateles strain 73 revealed a close relationship to Herpesvirus saimiri, with a high degree of variability within the left terminus of the coding region. A spliced mRNA transcribed from this region was detected in New World monkey T-cell lines transformed by Herpesvirus ateles in vitro or derived from T cells of infected Saguinus oedipus. The encoded viral protein, termed Tio, shows restricted homology to the oncoprotein StpC and to the tyrosine kinase-interacting protein Tip, two gene products responsible for the T-cell-transforming and oncogenic phenotype of Herpesvirus saimiri group C strains. Tio was detectable in lysates of the transformed T lymphocytes. Dimer formation was observed after expression of recombinant Tio. After cotransfection, Tio was phosphorylated in vivo by the protein tyrosine kinases Lck and Src and less efficiently by Fyn. Stable complexes of these Src family kinases with the viral protein were detected in lysates of the transfected cells. Binding analyses indicated a direct interaction of Tio with the SH3 domains of Lyn, Hck, Lck, Src, Fyn, and Yes. In addition, tyrosine-phosphorylated Tio bound to the SH2 domains of Lck, Src, or Fyn. Thus, Herpesvirus ateles-encoded Tio may contribute to viral T-cell transformation by influencing the function of Src family kinases.
Jens-christian Albrecht - One of the best experts on this subject based on the ideXlab platform.
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Herpesvirus Ateles Tio Can Replace Herpesvirus Saimiri StpC and Tip Oncoproteins in Growth Transformation of Monkey and Human T Cells
Journal of virology, 2004Co-Authors: Jens-christian Albrecht, Bernhard Fleckenstein, Brigitte Biesinger, Ingrid Müller-fleckenstein, Doris Lengenfelder, Monika Schmidt, Armin EnsserAbstract:Herpesvirus saimiri group C strains are capable of transforming human and simian T-lymphocyte populations to permanent antigen-independent growth. Two viral oncoproteins, StpC and Tip, that are encoded by a single bicistronic mRNA, act in concert to mediate this phenotype. A closely related New World monkey Herpesvirus, Herpesvirus ateles, transcribes a single spliced mRNA at an equivalent genome locus. The encoded protein, Tio, has sequence homologies to both StpC and Tip. We inserted the tio sequence of Herpesvirus ateles strain 73 into a recombinant Herpesvirus saimiri C488 lacking its own stpC/tip oncogene. Simian as well as human T lymphocytes were growth transformed by the chimeric Tio-expressing viruses. Thus, a single Herpesvirus protein appears to be responsible for the oncogenic effects of Herpesvirus ateles.
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Herpesvirus Ateles Gene Product Tio Interacts with Nonreceptor Protein Tyrosine Kinases
Journal of virology, 1999Co-Authors: Jens-christian Albrecht, Bernhard Fleckenstein, Ute Friedrich, Christian Kardinal, Jadranka Koehn, Stephan M. Feller, Brigitte BiesingerAbstract:Herpesvirus ateles is a gamma-2-Herpesvirus which naturally infects spider monkeys (Ateles spp.) and causes malignant lymphoproliferative disorders in various other New World primates. The genomic sequence of Herpesvirus ateles strain 73 revealed a close relationship to Herpesvirus saimiri, with a high degree of variability within the left terminus of the coding region. A spliced mRNA transcribed from this region was detected in New World monkey T-cell lines transformed by Herpesvirus ateles in vitro or derived from T cells of infected Saguinus oedipus. The encoded viral protein, termed Tio, shows restricted homology to the oncoprotein StpC and to the tyrosine kinase-interacting protein Tip, two gene products responsible for the T-cell-transforming and oncogenic phenotype of Herpesvirus saimiri group C strains. Tio was detectable in lysates of the transformed T lymphocytes. Dimer formation was observed after expression of recombinant Tio. After cotransfection, Tio was phosphorylated in vivo by the protein tyrosine kinases Lck and Src and less efficiently by Fyn. Stable complexes of these Src family kinases with the viral protein were detected in lysates of the transfected cells. Binding analyses indicated a direct interaction of Tio with the SH3 domains of Lyn, Hck, Lck, Src, Fyn, and Yes. In addition, tyrosine-phosphorylated Tio bound to the SH2 domains of Lck, Src, or Fyn. Thus, Herpesvirus ateles-encoded Tio may contribute to viral T-cell transformation by influencing the function of Src family kinases.
Mark D Schrenzel - One of the best experts on this subject based on the ideXlab platform.
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Malignant Catarrhal Fever-Like Disease in Barbary Red Deer (Cervus elaphus barbarus) Naturally Infected with a Virus Resembling Alcelaphine Herpesvirus 2
Journal of Clinical Microbiology, 2002Co-Authors: Robert B. Klieforth, Gabriel Maalouf, Donald L. Janssen, Ilse H. Stalis, Karen A. Terio, Mark D SchrenzelAbstract:Malignant catarrhal fever (MCF) is a systemic disease of ruminants caused by Herpesvirus. Infection with an MCF Herpesvirus is usually fatal, although some ruminants, such as wildebeest, hartebeest, and domestic sheep, are well adapted to particular strains of virus and function as clinically normal reservoirs (9, 13). MCF has been recognized in at least 13 species of deer, including mule deer (Odocoileus hemionus), Pere David's deer (Elaphurus davidianus), white-tailed deer (Odocoileus virginianus), and red deer (Cervus elaphus) (2, 18, 33, 36). Among these, molecular characterization of the causative virus has been done only for the white-tailed deer outbreaks from 1998 to 2001 (15, 18). In recent years the use of PCR primers for conserved regions of the Herpesvirus DNA polymerase gene has facilitated identification of previously unknown viruses (46). Among ruminants, these include bovine lymphotropic Herpesvirus (42), caprine Herpesvirus 2 (CpHV-2) (4, 19), caprine lymphotropic Herpesvirus (CpLHV) (19), and a Herpesvirus of unknown origin in white-tailed deer (Odocoileus virginianus) (18). Two of these, CpHV-2 and the Herpesvirus found in white-tailed deer, have been provisionally classified as members of the MCF group under Gammaherpesvirinae on the basis of a common antigenic epitope and DNA sequence similarity within the DNA polymerase gene (18, 19). CpHV-2 has been associated with chronic dermatitis and weight loss in sika deer (Cervus nippon) (19), while the virus found in white-tailed deer produced a disease with typical MCF clinical signs but had a prolonged course and chronic histologic lesions (18). These viruses are now included with alcelaphine Herpesvirus 1 (AlHV-1) and ovine Herpesvirus 2 (OvHV-2) as members of the MCF virus group with known pathogenic potential. AlHV-1, first described and linked to MCF in 1960 (31), can presently be detected by PCR (14, 17, 29) or by serologic methods (17, 20-22) in clinical MCF cases or herd screening. However, unambiguous identification of AlHV-1 may require sequencing of PCR-amplified DNA. OvHV-2 is similarly identifiable by a virus-specific PCR assay (1, 5, 23, 48) and by the same competitive-inhibition enzyme-linked immunosorbent assay (CI-ELISA) that detects antibodies to AlHV-1 (7, 20, 22). In contrast, two members of the MCF group have not been associated with disease: hippotragine Herpesvirus 1 (HiHV-1) (34) and alcelaphine Herpesvirus 2 (AlHV-2) (9, 13). The latter has been isolated several times from clinically normal topi or Cape hartebeest, members of the bovid subfamily Alcelaphinae (30, 38, 44, 45), but experimental infections of cattle produce no discernible effects; moreover, inoculation of cattle with AlHV-2 does not elicit antibodies protective against subsequent AlHV-1 challenge (30). AlHV-2 can be detected by AlHV-2-specific PCR as well as by a PCR that will amplify both AlHV-1 and AlHV-2 (14, 17, 29) and by CI-ELISA (19). We now report evidence that a virus closely related to AlHV-2 caused disease resembling MCF in eight Barbary red deer (Cervus elaphus barbarus) and that the virus may have derived from cohoused, clinically normal Jackson's hartebeest (Alcelaphus buselaphus jacksoni).
Hongwei Zhu - One of the best experts on this subject based on the ideXlab platform.
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evidence of two genetically different lymphotropic Herpesviruses present among red deer sambar and milu herds in china
Journal of Veterinary Science, 2018Co-Authors: Hongwei Zhu, Huitao Liu, Jianlong Zhang, Linlin Jiang, Guozhong Chen, Zhibin Feng, Tao Feng, Xingxiao ZhangAbstract:Herpesvirus infections in Cervidae are a serious threat affecting some deer species worldwide. In our attempt to identify malignant catarrhal fever-associated Herpesviruses in deer herds, ten gammaherpesviral DNA fragments were identified in five species of deer in herds in China by using a pan-Herpesvirus polymerase chain reaction assay targeting viral DNA polymerase. Notably, in sambar (Rusa unicolor), a novel gamma-2 Herpesvirus was identified that showed a close relationship with fallow deer lymphotropic Herpesvirus (LHV), while the other fragments were phylogenetically grouped together with Elk-LHV. Determination of whether these viruses have any clinical implication in these deer species should be undertaken urgently.
