The Experts below are selected from a list of 32316 Experts worldwide ranked by ideXlab platform

Jacques Moiroux - One of the best experts on this subject based on the ideXlab platform.

Agnes Anne - One of the best experts on this subject based on the ideXlab platform.

And Agnes Bouchardon - One of the best experts on this subject based on the ideXlab platform.

Ruichang Gao - One of the best experts on this subject based on the ideXlab platform.

  • effect of l histidine on the heat induced aggregation of bighead carp aristichthys nobilis myosin in low High Ionic Strength solution
    Food Hydrocolloids, 2018
    Co-Authors: Ruichang Gao, Yanmin Wang, Tong Shi, Li Yuan
    Abstract:

    Abstract In this study, the effects of l -histidine (L-His) on the aggregation of bighead carp ( Aristichthys nobilis ) myosin induced by heating in low (0.1) or High (0.5) Ionic Strength solutions were studied. The solubility of natural myosin was significantly enhanced by L-His both in low and High Ionic Strength solutions. More small particles are suspended in the heated solution containing L-His, inducing a Higher solubility than that solution without L-His. The surface hydrophobicity of myosin in a low Ionic Strength solution containing L-His is Higher than that of myosin without L-His. The results of morphology show that L-His induced myosin to form finer aggregates and a honeycomb-like network in a low/High Ionic Strength solution during heat treatment. These results demonstrate that L-His might disrupt the electrostatic property of unfolded myosin heat-induced through electrostatic binding with the exposed negatively charged amino acid residues, which might disarrange hydrophobic surfaces, resulting in weakening hydrophobic interactions, which ultimately suppresses the fierce aggregation of carp myosin.

Jing Wang - One of the best experts on this subject based on the ideXlab platform.

  • one step label free optical genosensing system for sequence specific dna related to the human immunodeficiency virus based on the measurements of light scattering signals of gold nanorods
    Analytical Chemistry, 2008
    Co-Authors: Cheng Zhi Huang, Jian Ping Xie, Rong Ge Yang, Pei Fu Zhou, Jing Wang
    Abstract:

    A one-step label-free optical genosensing method has been developed in this contribution by taking short DNA target with its sequence related to the human immunodeficiency virus type 1 (HIV-1) as an example. By employing anisotropic nonspherical and positively charged gold nanorods (Au-NRs) as the recognition platform, which show High stability against aggregation under High Ionic Strength conditions without any additional stable reagent, we found that the addition of target DNA to the mixture of nonmodified Au-NRs suspension and label-free probe DNA in High Ionic Strength buffer leads to a color change from red to light purple in less than 5 min, displaying strong plasmon resonance light scattering (PRLS) signals. Mechanism investigations showed that the strong PRLS signals should be ascribed to the aggregation of Au-NRs induced by the formed double-stranded oligonucleotides (dsDNA) from the hybridization of target DNA with probe DNA. With the PRLS signals, we monitored the hybridization process of a 21-...