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Heikki Kallio - One of the best experts on this subject based on the ideXlab platform.

  • fast analysis of sugars fruit acids and vitamin c in sea buckthorn Hippophae rhamnoides l varieties
    Journal of Agricultural and Food Chemistry, 2006
    Co-Authors: Katja Tiitinen, Baoru Yang, Gudmundur G Haraldsson, Sigridur Jonsdottir, Heikki Kallio
    Abstract:

    A fast, one-step gas chromatographic method was developed to analyze trimethylsilyl (TMS) derivatives of sugars, fruit acids, and ascorbic acid in sea buckthorn (Hippohae rhamnoides L.) berries. The method was applied to berry press juice of sea buckthorn of different origins grown in Finland during the 2003 and 2004 seasons. The method gave reliable results for d-fructose, d-glucose, ethyl-d-glucose, and malic, quinic, and ascorbic acids, which are the major sugars and acids in sea buckthorn juice. For the first time in sea buckthorn and evidently in any berry, the presence of ethyl β-d-glucopyranoside is reported. The structure of ethyl glucose was verified by high-performance liquid chromatography (HPLC), gas chromatography (GC), MS, and NMR analyses of both the isolated and the synthesized compounds. In the GC method, vitamin C was analyzed as ascorbic acid only, and dehydroascorbic acid was thus not taken into account. Keywords: Sea buckthorn (Hippophae rhamnoides L.); fruit acids; gas chromatography...

Rui Hai Liu - One of the best experts on this subject based on the ideXlab platform.

  • comparative assessment of phytochemical profiles antioxidant and antiproliferative activities of sea buckthorn Hippophae rhamnoides l berries
    Food Chemistry, 2017
    Co-Authors: Ruixue Guo, Xinbo Guo, Rui Hai Liu
    Abstract:

    Phytochemical profiles, antioxidant and antiproliferative activities of berry extracts were evaluated and compared in four subspecies of Sea buckthorn (Hippophae rhamnoides L.). Among the subspecies, Hippophae rhamnoides L. subsp. sinensis exhibited highest total phenolics content (38.7±1.3mgGA equiv./g DW) and corresponding total antioxidant activity. Whereas maximum cellular antioxidant and antiproliferative activities were determined in Hippophae rhamnoides L. subsp. yunnanensis. Total antioxidant activity was significantly associated to total phenolics, isorhamnetin-3-rutinoside and isorhamnetin-3-glucoside. The cellular antioxidant activity and antiproliferative activity of phytochemicals were fairly correlated to phenolic acids and flavonoid aglycones. Lower median effective dose (EC50) of individual compounds against human liver cancer HepG2 cells proliferation studies confirmed the better correlation between antiproliferative activity of Sea buckthorn extracts and flavonoid aglycones, including isorhamnetin, quercetin and kaempferol.

Katja Tiitinen - One of the best experts on this subject based on the ideXlab platform.

  • fast analysis of sugars fruit acids and vitamin c in sea buckthorn Hippophae rhamnoides l varieties
    Journal of Agricultural and Food Chemistry, 2006
    Co-Authors: Katja Tiitinen, Baoru Yang, Gudmundur G Haraldsson, Sigridur Jonsdottir, Heikki Kallio
    Abstract:

    A fast, one-step gas chromatographic method was developed to analyze trimethylsilyl (TMS) derivatives of sugars, fruit acids, and ascorbic acid in sea buckthorn (Hippohae rhamnoides L.) berries. The method was applied to berry press juice of sea buckthorn of different origins grown in Finland during the 2003 and 2004 seasons. The method gave reliable results for d-fructose, d-glucose, ethyl-d-glucose, and malic, quinic, and ascorbic acids, which are the major sugars and acids in sea buckthorn juice. For the first time in sea buckthorn and evidently in any berry, the presence of ethyl β-d-glucopyranoside is reported. The structure of ethyl glucose was verified by high-performance liquid chromatography (HPLC), gas chromatography (GC), MS, and NMR analyses of both the isolated and the synthesized compounds. In the GC method, vitamin C was analyzed as ascorbic acid only, and dehydroascorbic acid was thus not taken into account. Keywords: Sea buckthorn (Hippophae rhamnoides L.); fruit acids; gas chromatography...

Xinbo Guo - One of the best experts on this subject based on the ideXlab platform.

  • comparative assessment of phytochemical profiles antioxidant and antiproliferative activities of sea buckthorn Hippophae rhamnoides l berries
    Food Chemistry, 2017
    Co-Authors: Ruixue Guo, Xinbo Guo, Rui Hai Liu
    Abstract:

    Phytochemical profiles, antioxidant and antiproliferative activities of berry extracts were evaluated and compared in four subspecies of Sea buckthorn (Hippophae rhamnoides L.). Among the subspecies, Hippophae rhamnoides L. subsp. sinensis exhibited highest total phenolics content (38.7±1.3mgGA equiv./g DW) and corresponding total antioxidant activity. Whereas maximum cellular antioxidant and antiproliferative activities were determined in Hippophae rhamnoides L. subsp. yunnanensis. Total antioxidant activity was significantly associated to total phenolics, isorhamnetin-3-rutinoside and isorhamnetin-3-glucoside. The cellular antioxidant activity and antiproliferative activity of phytochemicals were fairly correlated to phenolic acids and flavonoid aglycones. Lower median effective dose (EC50) of individual compounds against human liver cancer HepG2 cells proliferation studies confirmed the better correlation between antiproliferative activity of Sea buckthorn extracts and flavonoid aglycones, including isorhamnetin, quercetin and kaempferol.

Gudmundur G Haraldsson - One of the best experts on this subject based on the ideXlab platform.

  • fast analysis of sugars fruit acids and vitamin c in sea buckthorn Hippophae rhamnoides l varieties
    Journal of Agricultural and Food Chemistry, 2006
    Co-Authors: Katja Tiitinen, Baoru Yang, Gudmundur G Haraldsson, Sigridur Jonsdottir, Heikki Kallio
    Abstract:

    A fast, one-step gas chromatographic method was developed to analyze trimethylsilyl (TMS) derivatives of sugars, fruit acids, and ascorbic acid in sea buckthorn (Hippohae rhamnoides L.) berries. The method was applied to berry press juice of sea buckthorn of different origins grown in Finland during the 2003 and 2004 seasons. The method gave reliable results for d-fructose, d-glucose, ethyl-d-glucose, and malic, quinic, and ascorbic acids, which are the major sugars and acids in sea buckthorn juice. For the first time in sea buckthorn and evidently in any berry, the presence of ethyl β-d-glucopyranoside is reported. The structure of ethyl glucose was verified by high-performance liquid chromatography (HPLC), gas chromatography (GC), MS, and NMR analyses of both the isolated and the synthesized compounds. In the GC method, vitamin C was analyzed as ascorbic acid only, and dehydroascorbic acid was thus not taken into account. Keywords: Sea buckthorn (Hippophae rhamnoides L.); fruit acids; gas chromatography...