Histochemistry

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Jürgen Roth - One of the best experts on this subject based on the ideXlab platform.

  • The Histochemistry and Cell Biology compendium: a review of 2012
    Histochemistry and Cell Biology, 2013
    Co-Authors: Douglas J. Taatjes, Jürgen Roth
    Abstract:

    The year 2012 was another exciting year for Histochemistry and Cell Biology . Innovations in immunohistochemical techniques and microscopy-based imaging have provided the means for advances in the field of cell biology. Over 130 manuscripts were published in the journal during 2012, representing methodological advancements, pathobiology of disease, and cell and tissue biology. This annual review of the manuscripts published in the previous year in Histochemistry and Cell Biology serves as an abbreviated reference for the readership to quickly peruse and discern trends in the field over the past year. The review has been broadly divided into multiple sections encompassing topics such as method advancements, subcellular components, extracellular matrix, and organ systems. We hope that the creation of this subdivision will serve to guide the reader to a specific topic of interest, while simultaneously providing a concise and easily accessible encapsulation of other topics in the broad area of Histochemistry and Cell Biology.

  • Recent progress in Histochemistry
    Histochemistry and Cell Biology, 2007
    Co-Authors: Christian Zuber, Douglas J. Taatjes, Jürgen Roth
    Abstract:

    The progress in discerning the structure and function of cells and tissues in health and disease has been achieved to a large extent by the continued development of new reagents for Histochemistry, the improvement of existing techniques and new imaging techniques. This review will highlight some advancements made in these fields.

  • Recent progress in Histochemistry and cell biology: the state of the art 2005
    Histochemistry and Cell Biology, 2005
    Co-Authors: Douglas J. Taatjes, Jürgen Roth
    Abstract:

    Advances in the field of Histochemistry, a multidisciplinary area including the detection, localization and functional characterization of molecules in single cells and complex tissues, often drives the attainment of new knowledge in the broadly defined discipline of cell biology. These two disciplines, Histochemistry and cell biology, have been joined in this journal to facilitate the flow of information with celerity from technical advancement in histochemical procedures, to their utilization in experimental models. This review summarizes advancements in these fields during the past year.

Douglas J. Taatjes - One of the best experts on this subject based on the ideXlab platform.

  • The Histochemistry and Cell Biology compendium: a review of 2012
    Histochemistry and Cell Biology, 2013
    Co-Authors: Douglas J. Taatjes, Jürgen Roth
    Abstract:

    The year 2012 was another exciting year for Histochemistry and Cell Biology . Innovations in immunohistochemical techniques and microscopy-based imaging have provided the means for advances in the field of cell biology. Over 130 manuscripts were published in the journal during 2012, representing methodological advancements, pathobiology of disease, and cell and tissue biology. This annual review of the manuscripts published in the previous year in Histochemistry and Cell Biology serves as an abbreviated reference for the readership to quickly peruse and discern trends in the field over the past year. The review has been broadly divided into multiple sections encompassing topics such as method advancements, subcellular components, extracellular matrix, and organ systems. We hope that the creation of this subdivision will serve to guide the reader to a specific topic of interest, while simultaneously providing a concise and easily accessible encapsulation of other topics in the broad area of Histochemistry and Cell Biology.

  • Recent progress in Histochemistry
    Histochemistry and Cell Biology, 2007
    Co-Authors: Christian Zuber, Douglas J. Taatjes, Jürgen Roth
    Abstract:

    The progress in discerning the structure and function of cells and tissues in health and disease has been achieved to a large extent by the continued development of new reagents for Histochemistry, the improvement of existing techniques and new imaging techniques. This review will highlight some advancements made in these fields.

  • Recent progress in Histochemistry and cell biology: the state of the art 2005
    Histochemistry and Cell Biology, 2005
    Co-Authors: Douglas J. Taatjes, Jürgen Roth
    Abstract:

    Advances in the field of Histochemistry, a multidisciplinary area including the detection, localization and functional characterization of molecules in single cells and complex tissues, often drives the attainment of new knowledge in the broadly defined discipline of cell biology. These two disciplines, Histochemistry and cell biology, have been joined in this journal to facilitate the flow of information with celerity from technical advancement in histochemical procedures, to their utilization in experimental models. This review summarizes advancements in these fields during the past year.

Eva Mezey - One of the best experts on this subject based on the ideXlab platform.

  • Hybridization Histochemistry of Neural Transcripts
    Current protocols in neuroscience, 2018
    Co-Authors: W. Scott Young, June Song, Eva Mezey
    Abstract:

    Expression of genes is manifested by the production of RNA transcripts within cells. Hybridization Histochemistry (or in situ hybridization) permits localization of these transcripts with cellular resolution or better. Furthermore, the relative amounts of transcripts detected in different tissues or in the same tissues in different states (e.g., physiological or developmental) may be quantified. This unit describes hybridization histochemical techniques using either oligodeoxynucleotide probes (see Basic Protocols 1 and 2, Alternate Protocol 1) or RNA probes (riboprobes; see Basic Protocols 3 and 5). These methods include a more recent approach using commercially available sets of oligodeoxynucleotide pairs for colorimetric and fluorescent detection (see Basic Protocol 2), as well as a method for detection of the Y chromosome using either mouse or human riboprobes (see Basic Protocol 5). Additional methods include colorimetric detection (see Basic Protocol 4) and tyramide signal amplification (TSA) of digoxigenin-labeled probes (see Alternate Protocol 2), and autoradiographic detection of radiolabeled probes (see Basic Protocol 6). Finally, methods are provided for labeling oligodeoxynucleotide (see Support Protocol 1) and RNA (see Support Protocol 2) probes, and verifying the probes by northern analysis (see Support Protocol 3).

Athina Efthymiadis - One of the best experts on this subject based on the ideXlab platform.

  • Recent progress in Histochemistry and cell biology
    Histochemistry and Cell Biology, 2012
    Co-Authors: Stefan Hübner, Athina Efthymiadis
    Abstract:

    Studies published in Histochemistry and Cell Biology in the year 2011 represent once more a manifest of established and newly sophisticated techniques being exploited to put tissue- and cell type-specific molecules into a functional context. The review is therefore the Histochemistry and Cell Biology’s yearly intention to provide interested readers appropriate summaries of investigations touching the areas of tissue biology, developmental biology, the biology of the immune system, stem cell research, the biology of subcellular compartments, in order to put the message of such studies into natural scientific-/human- and also pathological-relevant correlations.

W. Scott Young - One of the best experts on this subject based on the ideXlab platform.

  • Hybridization Histochemistry of Neural Transcripts
    Current protocols in neuroscience, 2018
    Co-Authors: W. Scott Young, June Song, Eva Mezey
    Abstract:

    Expression of genes is manifested by the production of RNA transcripts within cells. Hybridization Histochemistry (or in situ hybridization) permits localization of these transcripts with cellular resolution or better. Furthermore, the relative amounts of transcripts detected in different tissues or in the same tissues in different states (e.g., physiological or developmental) may be quantified. This unit describes hybridization histochemical techniques using either oligodeoxynucleotide probes (see Basic Protocols 1 and 2, Alternate Protocol 1) or RNA probes (riboprobes; see Basic Protocols 3 and 5). These methods include a more recent approach using commercially available sets of oligodeoxynucleotide pairs for colorimetric and fluorescent detection (see Basic Protocol 2), as well as a method for detection of the Y chromosome using either mouse or human riboprobes (see Basic Protocol 5). Additional methods include colorimetric detection (see Basic Protocol 4) and tyramide signal amplification (TSA) of digoxigenin-labeled probes (see Alternate Protocol 2), and autoradiographic detection of radiolabeled probes (see Basic Protocol 6). Finally, methods are provided for labeling oligodeoxynucleotide (see Support Protocol 1) and RNA (see Support Protocol 2) probes, and verifying the probes by northern analysis (see Support Protocol 3).