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Christian Dullin - One of the best experts on this subject based on the ideXlab platform.

  • X-ray-Based 3D Virtual Histology—Adding the Next Dimension to Histological Analysis
    Molecular Imaging and Biology, 2018
    Co-Authors: Jonas Albers, Serena Pacilè, Marlène Wiart, Giuliana Tromba, M. Markus, G. Vande Velde, Christian Dullin
    Abstract:

    Histology and immunohistochemistry of thin tissue sections have been the standard diagnostic procedure in many diseases for decades. This method is highly specific for particular tissue regions or cells, but mechanical sectioning of the specimens is required, which destroys the sample in the process and can lead to non-uniform tissue deformations. In addition, regions of interest cannot be located beforehand and the Analysis is intrinsically two-dimensional. Micro X-ray computed tomography (mu CT) on the other hand can provide 3D images at high resolution and allows for quantification of tissue structures, as well as the localization of small regions of interest. These advantages advocate the use of mu CT for virtual histology tool with or without subsequent classical histology. This review summarizes the most recent examples of virtual histology and provides currently known possibilities of improving contrast and resolution of mu CT. Following a background in mu CT imaging, ex vivo staining procedures for contrast enhancement are presented as well as label-free virtual histology approaches and the technologies, which could rapidly advance it, such as phase-contrast CT. Novel approaches such as zoom tomography and nanoparticulate contrast agents will also be considered. The current evidence suggests that virtual histology may present a valuable addition to the workflow of Histological Analysis, potentially reducing the workload in pathology, refining tissue classification, and supporting the detection of small malignancies.

  • X-ray-Based 3D Virtual Histology-Adding the Next Dimension to Histological Analysis.
    Molecular Imaging and Biology, 2018
    Co-Authors: Jonas Albers, Serena Pacilè, M. A. Markus, Marlène Wiart, G. Vande Velde, Giuliana Tromba, Christian Dullin
    Abstract:

    : Histology and immunohistochemistry of thin tissue sections have been the standard diagnostic procedure in many diseases for decades. This method is highly specific for particular tissue regions or cells, but mechanical sectioning of the specimens is required, which destroys the sample in the process and can lead to non-uniform tissue deformations. In addition, regions of interest cannot be located beforehand and the Analysis is intrinsically two-dimensional. Micro X-ray computed tomography (μCT) on the other hand can provide 3D images at high resolution and allows for quantification of tissue structures, as well as the localization of small regions of interest. These advantages advocate the use of μCT for virtual histology tool with or without subsequent classical histology. This review summarizes the most recent examples of virtual histology and provides currently known possibilities of improving contrast and resolution of μCT. Following a background in μCT imaging, ex vivo staining procedures for contrast enhancement are presented as well as label-free virtual histology approaches and the technologies, which could rapidly advance it, such as phase-contrast CT. Novel approaches such as zoom tomography and nanoparticulate contrast agents will also be considered. The current evidence suggests that virtual histology may present a valuable addition to the workflow of Histological Analysis, potentially reducing the workload in pathology, refining tissue classification, and supporting the detection of small malignancies.

Jiang Xinquan - One of the best experts on this subject based on the ideXlab platform.

  • Anatomic and Histological Analysis in a goat model used for maxillary sinus floor augmentation with simultaneous implant placement.
    Clinical Oral Implants Research, 2010
    Co-Authors: Zou Rong, Guo Lian, Lu Jiayu, Zhang Zhiyuan, Jiang Xinquan
    Abstract:

    Objectives: The aim of the present study was to carry out an anatomic survey on the goat maxillary sinus in order to provide accurate and definite anatomic parameters for the design of sinus floor elevation and dental implantation studies in this valuable preclinic animal model. Material and methods: The anatomic topographic structure of the maxillary sinuses was studied bilaterally in 10 adult goats by a gross survey as well as a Histological Analysis with parasagittal or coronal sections. Then following parameters were defined and measured: (1) maxillary alveolar height (MAH): vertical height from the alveolar crest to the sinus floor; (2) sinus lateral floor width (SLFW): horizontal distance from the lateral border of the anteroposterior bone crest to the sinus lateral wall; (3) infraorbital canal diameter (ICD); and (4) maxillary sinus volume (MSV): the volume occupied by water injected into the sinus. The data were presented with mean±SD on both sides. Results: The goat has a maxillary sinus similar to humans, with a slender pyramidal shape that pneumatizes the entire maxilla, and a sinus wall covered with a mucosal lining. From the maxillary sinus floor, there is an anteroposterior bone crest protruding with the infraorbital canal enveloped. It divides the maxillary sinus floor into two parts. The SLFW of the lateral part of the maxillary sinus floor becomes broader, about 5.905±1.475 mm in the third premolar site, and the MAH increases towards the posterior area, where the maxillary sinus floor is close to the related teeth roots. According to original metrical data, we also proposed a possible operation procedure for sinus floor augmentation. Conclusions: There is enough space in the lateral floor of the maxillary sinus for dental implantation, and the third premolar area might be a suitable position suggested for maxillary sinus augmentation with simultaneous implant placement in a goat model. To cite this article: Zou D, Guo L, Lu J, Zhang X, Zhang Z, Jiang X. Anatomic and Histological Analysis in a goat model used for maxillary sinus floor augmentation with simultaneous implant placement. Clin. Oral Impl. Res. 21, 2010; 65–70.

Jonas Albers - One of the best experts on this subject based on the ideXlab platform.

  • X-ray-Based 3D Virtual Histology—Adding the Next Dimension to Histological Analysis
    Molecular Imaging and Biology, 2018
    Co-Authors: Jonas Albers, Serena Pacilè, Marlène Wiart, Giuliana Tromba, M. Markus, G. Vande Velde, Christian Dullin
    Abstract:

    Histology and immunohistochemistry of thin tissue sections have been the standard diagnostic procedure in many diseases for decades. This method is highly specific for particular tissue regions or cells, but mechanical sectioning of the specimens is required, which destroys the sample in the process and can lead to non-uniform tissue deformations. In addition, regions of interest cannot be located beforehand and the Analysis is intrinsically two-dimensional. Micro X-ray computed tomography (mu CT) on the other hand can provide 3D images at high resolution and allows for quantification of tissue structures, as well as the localization of small regions of interest. These advantages advocate the use of mu CT for virtual histology tool with or without subsequent classical histology. This review summarizes the most recent examples of virtual histology and provides currently known possibilities of improving contrast and resolution of mu CT. Following a background in mu CT imaging, ex vivo staining procedures for contrast enhancement are presented as well as label-free virtual histology approaches and the technologies, which could rapidly advance it, such as phase-contrast CT. Novel approaches such as zoom tomography and nanoparticulate contrast agents will also be considered. The current evidence suggests that virtual histology may present a valuable addition to the workflow of Histological Analysis, potentially reducing the workload in pathology, refining tissue classification, and supporting the detection of small malignancies.

  • X-ray-Based 3D Virtual Histology-Adding the Next Dimension to Histological Analysis.
    Molecular Imaging and Biology, 2018
    Co-Authors: Jonas Albers, Serena Pacilè, M. A. Markus, Marlène Wiart, G. Vande Velde, Giuliana Tromba, Christian Dullin
    Abstract:

    : Histology and immunohistochemistry of thin tissue sections have been the standard diagnostic procedure in many diseases for decades. This method is highly specific for particular tissue regions or cells, but mechanical sectioning of the specimens is required, which destroys the sample in the process and can lead to non-uniform tissue deformations. In addition, regions of interest cannot be located beforehand and the Analysis is intrinsically two-dimensional. Micro X-ray computed tomography (μCT) on the other hand can provide 3D images at high resolution and allows for quantification of tissue structures, as well as the localization of small regions of interest. These advantages advocate the use of μCT for virtual histology tool with or without subsequent classical histology. This review summarizes the most recent examples of virtual histology and provides currently known possibilities of improving contrast and resolution of μCT. Following a background in μCT imaging, ex vivo staining procedures for contrast enhancement are presented as well as label-free virtual histology approaches and the technologies, which could rapidly advance it, such as phase-contrast CT. Novel approaches such as zoom tomography and nanoparticulate contrast agents will also be considered. The current evidence suggests that virtual histology may present a valuable addition to the workflow of Histological Analysis, potentially reducing the workload in pathology, refining tissue classification, and supporting the detection of small malignancies.

Howard J Eisen - One of the best experts on this subject based on the ideXlab platform.

  • autologous skeletal myoblasts transplanted to ischemia damaged myocardium in humans Histological Analysis of cell survival and differentiation
    Journal of the American College of Cardiology, 2003
    Co-Authors: Francis D Pagani, Harout Dersimonian, Agatha Zawadzka, Kristie Wetzel, Albert S B Edge, Douglas B Jacoby, Jonathan Dinsmore, Susan Wright, Tom Aretz, Howard J Eisen
    Abstract:

    Abstract Objectives We report Histological Analysis of hearts from patients with end-stage heart disease who were transplanted with autologous skeletal myoblasts concurrent with left ventricular assist device (LVAD) implantation. Background Autologous skeletal myoblast transplantation is under investigation as a means to repair infarcted myocardium. To date, there is only indirect evidence to suggest survival of skeletal muscle in humans. Methods Five patients (all male; median age 60 years) with ischemic cardiomyopathy, refractory heart failure, and listed for heart transplantation underwent muscle biopsy from the quadriceps muscle. The muscle specimen was shipped to a cell isolation facility where myoblasts were isolated and grown. Patients received a transplant of 300 million cells concomitant with LVAD implantation. Four patients underwent LVAD explant after 68, 91, 141, and 191 days of LVAD support (three transplant, one LVAD death), respectively. One patient remains alive on LVAD support awaiting heart transplantation. Results Skeletal muscle cell survival and differentiation into mature myofibers were directly demonstrated in scarred myocardium from three of the four explanted hearts using an antibody against skeletal muscle-specific myosin heavy chain. An increase in small vessel formation was observed in one of three patients at the site of surviving myotubes, but not in adjacent tissue devoid of engrafted cells. Conclusions These findings represent demonstration of autologous myoblast cell survival in human heart. The implanted skeletal myoblasts formed viable grafts in heavily scarred human myocardial tissue. These results establish the feasibility of myoblast transplants for myocardial repair in humans.

Zou Rong - One of the best experts on this subject based on the ideXlab platform.

  • Anatomic and Histological Analysis in a goat model used for maxillary sinus floor augmentation with simultaneous implant placement.
    Clinical Oral Implants Research, 2010
    Co-Authors: Zou Rong, Guo Lian, Lu Jiayu, Zhang Zhiyuan, Jiang Xinquan
    Abstract:

    Objectives: The aim of the present study was to carry out an anatomic survey on the goat maxillary sinus in order to provide accurate and definite anatomic parameters for the design of sinus floor elevation and dental implantation studies in this valuable preclinic animal model. Material and methods: The anatomic topographic structure of the maxillary sinuses was studied bilaterally in 10 adult goats by a gross survey as well as a Histological Analysis with parasagittal or coronal sections. Then following parameters were defined and measured: (1) maxillary alveolar height (MAH): vertical height from the alveolar crest to the sinus floor; (2) sinus lateral floor width (SLFW): horizontal distance from the lateral border of the anteroposterior bone crest to the sinus lateral wall; (3) infraorbital canal diameter (ICD); and (4) maxillary sinus volume (MSV): the volume occupied by water injected into the sinus. The data were presented with mean±SD on both sides. Results: The goat has a maxillary sinus similar to humans, with a slender pyramidal shape that pneumatizes the entire maxilla, and a sinus wall covered with a mucosal lining. From the maxillary sinus floor, there is an anteroposterior bone crest protruding with the infraorbital canal enveloped. It divides the maxillary sinus floor into two parts. The SLFW of the lateral part of the maxillary sinus floor becomes broader, about 5.905±1.475 mm in the third premolar site, and the MAH increases towards the posterior area, where the maxillary sinus floor is close to the related teeth roots. According to original metrical data, we also proposed a possible operation procedure for sinus floor augmentation. Conclusions: There is enough space in the lateral floor of the maxillary sinus for dental implantation, and the third premolar area might be a suitable position suggested for maxillary sinus augmentation with simultaneous implant placement in a goat model. To cite this article: Zou D, Guo L, Lu J, Zhang X, Zhang Z, Jiang X. Anatomic and Histological Analysis in a goat model used for maxillary sinus floor augmentation with simultaneous implant placement. Clin. Oral Impl. Res. 21, 2010; 65–70.