X-Ray Computed Tomography

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Sacha J. Mooney - One of the best experts on this subject based on the ideXlab platform.

  • x ray Computed Tomography of crop plant root systems grown in soil
    Current protocols in plant biology, 2017
    Co-Authors: Stefan Mairhofer, Sacha J. Mooney, Tony P Pridmore, James Johnson, Darren M Wells, Malcolm J Bennett, Craig J. Sturrock
    Abstract:

    Non-destructive methods to quantify the root system architecture of a plant grown in soil are essential to aid our understanding of the factors that impact plant root development in natural environments. With environmental change threatening our ability to sustain agricultural productivity for an expanding global population, the application of these methods has never before seen such an increase in demand. X-Ray Computed Tomography (CT) based phenotyping techniques permit the spatio-temporal quantification of roots, helping to identify novel adaptive root architectural responses to abiotic and biotic factors. This protocol reports an integrated workflow from column preparation and plant growth to image and quantification of the root system using novel open source software applications, RooTrak and RooTh. © 2017 by John Wiley & Sons, Inc.

  • Quantification of seed–soil contact of sugar beet (Beta vulgaris) using X-Ray Computed Tomography
    Plant Methods, 2017
    Co-Authors: Sebastian Blunk, Martine I. Heer, Debbie Sparkes, Kenneth Fredlund, Ali Hafeez Malik, Jennifer Bussell, Tobias Ekblad, Craig J. Sturrock, Sacha J. Mooney
    Abstract:

    BackgroundSeed–soil contact is important to ensure successful germination, however, there is a paucity of reported studies that have quantified the microstructure at and around this critical interface, mainly due to the opacity of soil.ResultsHere we describe a novel methodology to non-destructively calculate the seed–soil contact area using X-Ray Computed Tomography. Under controlled conditions, we observed that seed–soil contact was strongly influenced by the size and type of seed, with a seed–soil contact of ca. 15% for naked sugar beet seeds compared to ca. 32% for pelleted and coated seeds. Similar results were obtained for seeds sampled from the field albeit with a higher spatial variability.ConclusionsBy application of this new quantification method it is hoped seed enhancement technologies can be optimised and ultimately seedbed preparation improved to ensure better germination.

  • quantification of seed soil contact of sugar beet beta vulgaris using x ray Computed Tomography
    Plant Methods, 2017
    Co-Authors: Sebastian Blunk, Martine I. Heer, Kenneth Fredlund, Ali Hafeez Malik, Jennifer Bussell, Tobias Ekblad, Craig J. Sturrock, Debbie L Sparkes, Sacha J. Mooney
    Abstract:

    Seed–soil contact is important to ensure successful germination, however, there is a paucity of reported studies that have quantified the microstructure at and around this critical interface, mainly due to the opacity of soil. Here we describe a novel methodology to non-destructively calculate the seed–soil contact area using X-Ray Computed Tomography. Under controlled conditions, we observed that seed–soil contact was strongly influenced by the size and type of seed, with a seed–soil contact of ca. 15% for naked sugar beet seeds compared to ca. 32% for pelleted and coated seeds. Similar results were obtained for seeds sampled from the field albeit with a higher spatial variability. By application of this new quantification method it is hoped seed enhancement technologies can be optimised and ultimately seedbed preparation improved to ensure better germination.

Martin Lepage - One of the best experts on this subject based on the ideXlab platform.

  • an experimental study of the dose response of polymer gel dosimeters imaged with x ray Computed Tomography
    Physics in Medicine and Biology, 2001
    Co-Authors: Jamie Trapp, Sven Back, Martin Lepage, Gregory Michael, Clair Baldock
    Abstract:

    Changes in the linear attenuation coefficient of polymer gel dosimeters post-irradiation enable the imaging of dose distributions by X-Ray Computed Tomography (CT). Various compositions of polymer gel dosimeters manufactured from acrylamide (AA), and N,N'-methylene-bis-acrylamide (BIS) comonomers and gelatin or agarose gelling agents were investigated. This work shows that increasing the comonomer concentration increases the CT-dose sensitivity of the polymer gel dosimeter. This can be further increased by replacing gelatin with agarose. Varying the gelatin concentration however does not significantly change the CT-dose sensitivity. Among the compositions studied, dose resolution (D(delta)95%) was found to be optimal for polymer gel dosimeters comprising 5% gelatin, 3% AA, 3% BIS and 89% water. (Less)

  • an experimental study of the dose response of polymer gel dosimeters imaged with x ray Computed Tomography
    Physics in Medicine and Biology, 2001
    Co-Authors: Jamie Trapp, Sven Back, Martin Lepage, Gregory Michael, Clive Baldock
    Abstract:

    Changes in the linear attenuation coefficient of polymer gel dosimeters post-irradiation enable the imaging of dose distributions by X-Ray Computed Tomography (CT). Various compositions of polymer gel dosimeters manufactured from acrylamide (AA), and N,N'-methylene-bis-acrylamide (BIS) comonomers and gelatin or agarose gelling agents were investigated. This work shows that increasing the comonomer concentration increases the CT-dose sensitivity of the polymer gel dosimeter. This can be further increased by replacing gelatin with agarose. Varying the gelatin concentration however does not significantly change the CT-dose sensitivity. Among the compositions studied, dose resolution (D(delta)95%) was found to be optimal for polymer gel dosimeters comprising 5% gelatin, 3% AA, 3% BIS and 89% water.

Sven Back - One of the best experts on this subject based on the ideXlab platform.

  • an experimental study of the dose response of polymer gel dosimeters imaged with x ray Computed Tomography
    Physics in Medicine and Biology, 2001
    Co-Authors: Jamie Trapp, Sven Back, Martin Lepage, Gregory Michael, Clair Baldock
    Abstract:

    Changes in the linear attenuation coefficient of polymer gel dosimeters post-irradiation enable the imaging of dose distributions by X-Ray Computed Tomography (CT). Various compositions of polymer gel dosimeters manufactured from acrylamide (AA), and N,N'-methylene-bis-acrylamide (BIS) comonomers and gelatin or agarose gelling agents were investigated. This work shows that increasing the comonomer concentration increases the CT-dose sensitivity of the polymer gel dosimeter. This can be further increased by replacing gelatin with agarose. Varying the gelatin concentration however does not significantly change the CT-dose sensitivity. Among the compositions studied, dose resolution (D(delta)95%) was found to be optimal for polymer gel dosimeters comprising 5% gelatin, 3% AA, 3% BIS and 89% water. (Less)

  • an experimental study of the dose response of polymer gel dosimeters imaged with x ray Computed Tomography
    Physics in Medicine and Biology, 2001
    Co-Authors: Jamie Trapp, Sven Back, Martin Lepage, Gregory Michael, Clive Baldock
    Abstract:

    Changes in the linear attenuation coefficient of polymer gel dosimeters post-irradiation enable the imaging of dose distributions by X-Ray Computed Tomography (CT). Various compositions of polymer gel dosimeters manufactured from acrylamide (AA), and N,N'-methylene-bis-acrylamide (BIS) comonomers and gelatin or agarose gelling agents were investigated. This work shows that increasing the comonomer concentration increases the CT-dose sensitivity of the polymer gel dosimeter. This can be further increased by replacing gelatin with agarose. Varying the gelatin concentration however does not significantly change the CT-dose sensitivity. Among the compositions studied, dose resolution (D(delta)95%) was found to be optimal for polymer gel dosimeters comprising 5% gelatin, 3% AA, 3% BIS and 89% water.

Sebastian Blunk - One of the best experts on this subject based on the ideXlab platform.

  • Quantification of seed–soil contact of sugar beet (Beta vulgaris) using X-Ray Computed Tomography
    Plant Methods, 2017
    Co-Authors: Sebastian Blunk, Martine I. Heer, Debbie Sparkes, Kenneth Fredlund, Ali Hafeez Malik, Jennifer Bussell, Tobias Ekblad, Craig J. Sturrock, Sacha J. Mooney
    Abstract:

    BackgroundSeed–soil contact is important to ensure successful germination, however, there is a paucity of reported studies that have quantified the microstructure at and around this critical interface, mainly due to the opacity of soil.ResultsHere we describe a novel methodology to non-destructively calculate the seed–soil contact area using X-Ray Computed Tomography. Under controlled conditions, we observed that seed–soil contact was strongly influenced by the size and type of seed, with a seed–soil contact of ca. 15% for naked sugar beet seeds compared to ca. 32% for pelleted and coated seeds. Similar results were obtained for seeds sampled from the field albeit with a higher spatial variability.ConclusionsBy application of this new quantification method it is hoped seed enhancement technologies can be optimised and ultimately seedbed preparation improved to ensure better germination.

  • quantification of seed soil contact of sugar beet beta vulgaris using x ray Computed Tomography
    Plant Methods, 2017
    Co-Authors: Sebastian Blunk, Martine I. Heer, Kenneth Fredlund, Ali Hafeez Malik, Jennifer Bussell, Tobias Ekblad, Craig J. Sturrock, Debbie L Sparkes, Sacha J. Mooney
    Abstract:

    Seed–soil contact is important to ensure successful germination, however, there is a paucity of reported studies that have quantified the microstructure at and around this critical interface, mainly due to the opacity of soil. Here we describe a novel methodology to non-destructively calculate the seed–soil contact area using X-Ray Computed Tomography. Under controlled conditions, we observed that seed–soil contact was strongly influenced by the size and type of seed, with a seed–soil contact of ca. 15% for naked sugar beet seeds compared to ca. 32% for pelleted and coated seeds. Similar results were obtained for seeds sampled from the field albeit with a higher spatial variability. By application of this new quantification method it is hoped seed enhancement technologies can be optimised and ultimately seedbed preparation improved to ensure better germination.

Jamie Trapp - One of the best experts on this subject based on the ideXlab platform.

  • an experimental study of the dose response of polymer gel dosimeters imaged with x ray Computed Tomography
    Physics in Medicine and Biology, 2001
    Co-Authors: Jamie Trapp, Sven Back, Martin Lepage, Gregory Michael, Clair Baldock
    Abstract:

    Changes in the linear attenuation coefficient of polymer gel dosimeters post-irradiation enable the imaging of dose distributions by X-Ray Computed Tomography (CT). Various compositions of polymer gel dosimeters manufactured from acrylamide (AA), and N,N'-methylene-bis-acrylamide (BIS) comonomers and gelatin or agarose gelling agents were investigated. This work shows that increasing the comonomer concentration increases the CT-dose sensitivity of the polymer gel dosimeter. This can be further increased by replacing gelatin with agarose. Varying the gelatin concentration however does not significantly change the CT-dose sensitivity. Among the compositions studied, dose resolution (D(delta)95%) was found to be optimal for polymer gel dosimeters comprising 5% gelatin, 3% AA, 3% BIS and 89% water. (Less)

  • an experimental study of the dose response of polymer gel dosimeters imaged with x ray Computed Tomography
    Physics in Medicine and Biology, 2001
    Co-Authors: Jamie Trapp, Sven Back, Martin Lepage, Gregory Michael, Clive Baldock
    Abstract:

    Changes in the linear attenuation coefficient of polymer gel dosimeters post-irradiation enable the imaging of dose distributions by X-Ray Computed Tomography (CT). Various compositions of polymer gel dosimeters manufactured from acrylamide (AA), and N,N'-methylene-bis-acrylamide (BIS) comonomers and gelatin or agarose gelling agents were investigated. This work shows that increasing the comonomer concentration increases the CT-dose sensitivity of the polymer gel dosimeter. This can be further increased by replacing gelatin with agarose. Varying the gelatin concentration however does not significantly change the CT-dose sensitivity. Among the compositions studied, dose resolution (D(delta)95%) was found to be optimal for polymer gel dosimeters comprising 5% gelatin, 3% AA, 3% BIS and 89% water.