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Maria Lucia Taylor - One of the best experts on this subject based on the ideXlab platform.
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Interaction of murine macrophage-membrane proteins with components of the pathogenic fungus Histoplasma capsulatum
Clinical and experimental immunology, 1998Co-Authors: Maria Lucia Taylor, M. R. Reyes-montes, Esperanza Duarte-escalante, N. Elizondo, Guadalupe Maldonado, Edgar ZentenoAbstract:The interaction of macrophage-membrane proteins and Histoplasmin, a crude antigen of the pathogenic fungus Histoplasma capsulatum, was studied using murine peritoneal macrophages. Membrane proteins were purified via membrane attachment to polycationic beads and solubilized in Tris-HCl/SDS/DTT/glycerol for protein extraction; afterwards they were adsorbed or not with H. capsulatum yeast or lectin binding-enriched by affinity chromatography. Membrane proteins and Histoplasmin interactions were detected by ELISA and immunoblotting assays using anti-H. capsulatum human or mouse serum and biotinylated goat anti-human or anti-mouse IgG/streptavidin-peroxidase system to reveal the interaction. Results indicate that macrophage-membrane proteins and Histoplasmin components interact in a dose-dependent reaction, and adsorption of macrophage-membrane proteins by yeast cells induces a critical decrease in the interaction. Macrophage-membrane glycoproteins with terminal D-galactosyl residues, purified by chromatography with Abrus precatorius lectin, bound to Histoplasmin; and two bands of 68kD and 180kD of transferred membrane protein samples interacted with Histoplasmin components, as revealed by immunoblot assays. Specificity for beta-galactoside residues on the macrophage-membrane was confirmed by galactose inhibition of the interaction between macrophage-membrane proteins and Histoplasmin components, in competitive ELISA using sugars, as well as by enzymatic cleavage of the galactoside residues.
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The syndrome of presumed ocular histoplasmosis in Mexico: a preliminary study
Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology, 1994Co-Authors: M. Pedroza-seres, Hugo Quiroz-mercado, Julio Granados, Maria Lucia TaylorAbstract:A study to screen for the syndrome of presumed ocular histoplasmosis (SPOH) among native populations from three Mexican states was performed. Two of these states, Guerrero and Queretaro, were selected as histoplasmosis is endemic there, whereas Tlaxcala was considered a control, due to the absence of reported cases. A total of 253 individuals were submitted to ocular fundus examination to obtain evidence of SPOH. A high percentage of positive reactors to Histoplasmin skin test (ST) was observed in Guerrero (83%) and Queretaro (53%), whereas in Tlaxcala positive ST were almost absent (2·04%). Only five individuals had retinal lesions, although these lesions were not characteristic of the syndrome. Stimulation of these individual's cells showed different patterns in the Histoplasmin-induced lymphocyte transformation response, and two out of five individuals with retinal lesions presented a stimulated response, as well as three controls without lesions. Histocompatibility antigens (HLA) were determined in a ...
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Two-dimensional immunoelectrophoresis of Histoplasmin and a purified polysaccharide-protein antigen of Histoplasma capsulatum.
Mycopathologia, 1993Co-Authors: C. Toriello, J. A. Jiménez-montiel, María Del Rocío Reyes-montes, Maria Lucia TaylorAbstract:Crude Histoplasmin and a polysaccharide-protein complex (PPC-histo) antigens obtained from culture filtrates ofHistoplasma capsulatum were analyzed by single and tandem two-dimensional immunoelectrophoresis (TD-IEP) using a rabbit hyperimmune anti-Histoplasmin polyvalent serum. Single TD-IEP showed 14 arc precipitates for Histoplasmin. Continuity of arcs 2, 6, and 7, and 9 and 10 was observed, suggesting a different polymeric configuration of the same antigen. This was also confirmed in tandem TD-IEP of Histoplasmin with homologous (PPC-histo) and heterologous PPC's fromBlastomyces dermatitidis, Paracoccidioides brasiliensis andCoccidioides immitis. Tandem TD-IEP of Histoplasmin and PPC-histo displayed a similar antigenic pattern to Histoplasmin alone, being arcs 1 and 3 more evident and apparently present only in Histoplasmin and PPC-histo. Tandem TD-IEP showed common antigens among the other heterologous fungal purified antigens, and seems useful to observe the multiplicity of antigens present in fungal preparations and to identify those precipitates (arcs 1 and 3) that are predominant in the purified preparation.
M. R. Reyes-montes - One of the best experts on this subject based on the ideXlab platform.
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Interaction of murine macrophage-membrane proteins with components of the pathogenic fungus Histoplasma capsulatum
Clinical and experimental immunology, 1998Co-Authors: Maria Lucia Taylor, M. R. Reyes-montes, Esperanza Duarte-escalante, N. Elizondo, Guadalupe Maldonado, Edgar ZentenoAbstract:The interaction of macrophage-membrane proteins and Histoplasmin, a crude antigen of the pathogenic fungus Histoplasma capsulatum, was studied using murine peritoneal macrophages. Membrane proteins were purified via membrane attachment to polycationic beads and solubilized in Tris-HCl/SDS/DTT/glycerol for protein extraction; afterwards they were adsorbed or not with H. capsulatum yeast or lectin binding-enriched by affinity chromatography. Membrane proteins and Histoplasmin interactions were detected by ELISA and immunoblotting assays using anti-H. capsulatum human or mouse serum and biotinylated goat anti-human or anti-mouse IgG/streptavidin-peroxidase system to reveal the interaction. Results indicate that macrophage-membrane proteins and Histoplasmin components interact in a dose-dependent reaction, and adsorption of macrophage-membrane proteins by yeast cells induces a critical decrease in the interaction. Macrophage-membrane glycoproteins with terminal D-galactosyl residues, purified by chromatography with Abrus precatorius lectin, bound to Histoplasmin; and two bands of 68kD and 180kD of transferred membrane protein samples interacted with Histoplasmin components, as revealed by immunoblot assays. Specificity for beta-galactoside residues on the macrophage-membrane was confirmed by galactose inhibition of the interaction between macrophage-membrane proteins and Histoplasmin components, in competitive ELISA using sugars, as well as by enzymatic cleavage of the galactoside residues.
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Two-dimensional immunoelectrophoresis of Histoplasmin and a purified polysaccharide-protein antigen ofHistoplasma capsulatum
Mycopathologia, 1993Co-Authors: C. Toriello, J. A. Jiménez-montiel, M. R. Reyes-montes, M. L. TaylorAbstract:Crude Histoplasmin and a polysaccharide-protein complex (PPC-histo) antigens obtained from culture filtrates of Histoplasma capsulatum were analyzed by single and tandem two-dimensional immunoelectrophoresis (TD-IEP) using a rabbit hyperimmune anti-Histoplasmin polyvalent serum. Single TD-IEP showed 14 arc precipitates for Histoplasmin. Continuity of arcs 2, 6, and 7, and 9 and 10 was observed, suggesting a different polymeric configuration of the same antigen. This was also confirmed in tandem TD-IEP of Histoplasmin with homologous (PPC-histo) and heterologous PPC's from Blastomyces dermatitidis, Paracoccidioides brasiliensis and Coccidioides immitis . Tandem TD-IEP of Histoplasmin and PPC-histo displayed a similar antigenic pattern to Histoplasmin alone, being arcs 1 and 3 more evident and apparently present only in Histoplasmin and PPC-histo. Tandem TD-IEP showed common antigens among the other heterologous fungal purified antigens, and seems useful to observe the multiplicity of antigens present in fungal preparations and to identify those precipitates (arcs 1 and 3) that are predominant in the purified preparation.
Miguel Suarez Hernandez - One of the best experts on this subject based on the ideXlab platform.
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Relación entre la prueba intradermica de Histoplasmina y los niveles de anticuerpos detectables por ELISA e immunodifusion Relationship between Histoplasmin skin test and Histoplasma capsulatum antibody levels detected by ELISA and immunodiffusion te
Universidade de São Paulo, 1994Co-Authors: Carlos M. Fernandez-andreu, Ana Margarita Cadre-raton, Gerardo Martinez Machin, Alina Llop Hernandez, Miguel Suarez HernandezAbstract:Se realizó un estudio prospectivo en 40 trabajadores de una granja avícola (grupo 1) considerados con riesgo de exposición a Histoplasma capsulatum, agente etiológico de la histoplasmosis, y en 16 individuos sin riesgo profesional de exposición a dicho agente (grupo 2). En ambos grupos se aplicó la prueba intradérmica de Histoplasmina y se obtuvo el suero antes de su aplicación y a los 30 y 180 días después de realizada dicha prueba. Se determinó el nivel de anticuerpos anti-H. capsulatum mediante las técnicas de ELISA e inmunodifusión doble. En los dos grupos de población estudiados la aplicación intradérmica de Histoplasmina, aún en los casos en que la respuesta fue positiva, no constituyó un estímulo antigénico suficiente para provocar un aumento en los niveles de anticuerpos anti-H. capsulation detectables por las técnicas serológicas empleadas. Los resultados obtenidos contribuyen a la mejor interpretación de la prueba de ELISA en el diagnóstico de la histoplasmosis.A prospective study was carried out in two groups of individuals: a group 1 (n=40) included workers from a poultry farm, with potencial occupational risk of exposure to Histoplasma capsulatum, ethiologic agent of histoplasmosis, and a group 2 (n=16), persons without occupational risk of exposure to the agent. Histoplasmin skin test was performed in both groups, and three sera were obtained from each individual: 1) before skin test was done, 2) 30 days after, and 3) 180 days after it. In both groups the Histoplasmin skin test, even when the test was positive, was not a sufficient antigenic booster to provoque an increase in the H. capsulatum antibody levels capable to be detected by the Serologic tests used (ELISA and Double Immunodiffusion). These results contribute to improve the interpretation of ELISA test values in the diagnosis of histoplasmosis
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Relationship between Histoplasmin skin test and Histoplasma capsulatum antibody levels detected by ELISA and immunodiffusion tests
Revista do Instituto de Medicina Tropical de Sao Paulo, 1994Co-Authors: Carlos M. Fernandez-andreu, Ana Margarita Cadre-raton, Gerardo Martinez Machin, Alina Llop Hernandez, Miguel Suarez HernandezAbstract:A prospective study was carried out in two groups of individuals: a group 1 (n=40) included workers from a poultry farm, with potencial occupational risk of exposure to Histoplasma capsulatum, ethiologic agent of histoplasmosis, and a group 2 (n=16), persons without occupational risk of exposure to the agent. Histoplasmin skin test was performed in both groups, and three sera were obtained from each individual: 1) before skin test was done, 2) 30 days after, and 3) 180 days after it. In both groups the Histoplasmin skin test, even when the test was positive, was not a sufficient antigenic booster to provoque an increase in the H. capsulatum antibody levels capable to be detected by the Serologic tests used (ELISA and Double Immunodiffusion). These results contribute to improve the interpretation of ELISA test values in the diagnosis of histoplasmosis.
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Reactividad a la Histoplasmina en trabajadores de granjas avícolas en la provincia de Ciego de Avila, Cuba Hipersensitivity to Histoplasmin in poultry farmers in the province of Ciego de Avila, Cuba
Universidade de São Paulo, 1992Co-Authors: Miguel Suarez Hernandez, Carlos Fernandez M. Andreu, Alfredo Estrada Ortiz, Eugenio Cisneros DespaigneAbstract:Se realizo una encuesta epidemiológica mediante la prueba intradérmica de Histoplasmina a 392 trabajadores de granjas avícolas y 265 de diferentes centros ocupacionales considerados sin riesgo profesional de exposición a Histoplasma capsulatum, agente etiológico de la histoplasinosis. Entre los trabajadores del primer grupo se detectó un 28,8% de positividad, mientras que en el segundo grupo se encontro un 13,2% de casos positivos; desde el punto de vista estadístico existió diferencia significativa entre ambos, lo que demuestra que los trabajadores de granjas avícolas son un grupo de riesgo de adquirir la infección por H capsulatum. Entre los trabajadores del grupo I se encontro un porcentaje de reactividad más alto en aquellos grupos ocupacionales que estaban más en contacto con las excretas de las aves. El tiempo de trabajo en la granja también parece influir en la reactividad a la prueba.An epidemiological survey with Histoplasmin skin test was performed in 392 poultry farmers and 265 workers considered without occupational risk of exposition to Histoplasma capsulatum, etiologic agent of histoplasmosis. The results were positive in 28.8% and 13.2% in both groups respectively. Statistically, there was a significant difference between the two groups, so it can be considered that poultry farmers are in occupational risk of infection with H. capsulatum. In the first group, the workers which are more closely related with chicken manure showed a higher reactivity to Histoplasmin skin test. The working time in the farms seems to influence in the test reactivity too
C. Toriello - One of the best experts on this subject based on the ideXlab platform.
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Participants sociodemographic, clinical features and associated factors in relation to Histoplasmin reactivity.
2018Co-Authors: Rita O. Oladele, C. Toriello, Folasade T. Ogunsola, Olusola O. Ayanlowo, Philip Foden, Adetona S. Fayemiwo, Iriagbonse I. Osaigbovo, Anthony A. Iwuafor, Shuwaram Shettima, Halimat A. EkundayoAbstract:Participants sociodemographic, clinical features and associated factors in relation to Histoplasmin reactivity.
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Two-dimensional immunoelectrophoresis of Histoplasmin and a purified polysaccharide-protein antigen ofHistoplasma capsulatum
Mycopathologia, 1993Co-Authors: C. Toriello, J. A. Jiménez-montiel, M. R. Reyes-montes, M. L. TaylorAbstract:Crude Histoplasmin and a polysaccharide-protein complex (PPC-histo) antigens obtained from culture filtrates of Histoplasma capsulatum were analyzed by single and tandem two-dimensional immunoelectrophoresis (TD-IEP) using a rabbit hyperimmune anti-Histoplasmin polyvalent serum. Single TD-IEP showed 14 arc precipitates for Histoplasmin. Continuity of arcs 2, 6, and 7, and 9 and 10 was observed, suggesting a different polymeric configuration of the same antigen. This was also confirmed in tandem TD-IEP of Histoplasmin with homologous (PPC-histo) and heterologous PPC's from Blastomyces dermatitidis, Paracoccidioides brasiliensis and Coccidioides immitis . Tandem TD-IEP of Histoplasmin and PPC-histo displayed a similar antigenic pattern to Histoplasmin alone, being arcs 1 and 3 more evident and apparently present only in Histoplasmin and PPC-histo. Tandem TD-IEP showed common antigens among the other heterologous fungal purified antigens, and seems useful to observe the multiplicity of antigens present in fungal preparations and to identify those precipitates (arcs 1 and 3) that are predominant in the purified preparation.
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Two-dimensional immunoelectrophoresis of Histoplasmin and a purified polysaccharide-protein antigen of Histoplasma capsulatum.
Mycopathologia, 1993Co-Authors: C. Toriello, J. A. Jiménez-montiel, María Del Rocío Reyes-montes, Maria Lucia TaylorAbstract:Crude Histoplasmin and a polysaccharide-protein complex (PPC-histo) antigens obtained from culture filtrates ofHistoplasma capsulatum were analyzed by single and tandem two-dimensional immunoelectrophoresis (TD-IEP) using a rabbit hyperimmune anti-Histoplasmin polyvalent serum. Single TD-IEP showed 14 arc precipitates for Histoplasmin. Continuity of arcs 2, 6, and 7, and 9 and 10 was observed, suggesting a different polymeric configuration of the same antigen. This was also confirmed in tandem TD-IEP of Histoplasmin with homologous (PPC-histo) and heterologous PPC's fromBlastomyces dermatitidis, Paracoccidioides brasiliensis andCoccidioides immitis. Tandem TD-IEP of Histoplasmin and PPC-histo displayed a similar antigenic pattern to Histoplasmin alone, being arcs 1 and 3 more evident and apparently present only in Histoplasmin and PPC-histo. Tandem TD-IEP showed common antigens among the other heterologous fungal purified antigens, and seems useful to observe the multiplicity of antigens present in fungal preparations and to identify those precipitates (arcs 1 and 3) that are predominant in the purified preparation.
Rosely Maria Zancopeoliveira - One of the best experts on this subject based on the ideXlab platform.
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evaluation of an enzyme linked immunosorbent assay using purified deglycosylated Histoplasmin for different clinical manifestations of histoplasmosis
Microbiological Research, 2010Co-Authors: Allan J Guimaraes, Claudia Vera Pizzini, Marcos De Abreu Almeida, Jose Mauro Peralta, Joshua D Nosanchuk, Rosely Maria ZancopeoliveiraAbstract:Diagnosis of invasive fungal diseases remains problematic, especially in undeveloped countries. We have developed an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Histoplasma capsulatum using metaperiodate treated purified Histoplasmin (ptHMIN). Our ELISA was validated comparing sera from patients with histoplasmosis, related mycoses, and healthy individuals. The overall test specificity was 96%, with sensitivities of 100% (8/8) in acute disease, 90% (9/10) in chronic disease, 89% (8/9) in disseminated infection in individuals without HIV infection, 86% (12/14) in disseminated disease in the setting of HIV infection and 100% (3/3) in mediastinal histoplasmosis. These parameters are superior to the use of untreated Histoplasmin in diagnostic ELISAs. The high specificities, sensitivities, and simplicity of our ELISA support further development of a deglycosylated HMIN ELISA for clinical use and for monitoring the humoral immune response during therapy in patients with chronic and disseminated histoplasmosis.