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C. Darke - One of the best experts on this subject based on the ideXlab platform.
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A serological study of the <B>HLAB>-B17 cross-reactive group.
Tissue Antigens, 2008Co-Authors: C. DarkeAbstract:: The <B>HLAB>-B17 cross-reactive group and the participation of the suBdivisions of B17 ( Bw57 and Bw58 ) in cross-reactivity were investigated By the serological analysis of 81 cytotoxic <B>HLAB> antisera (produced By pregnancy alone), the <B>HLAB> typing of the antiserum donors and the identification of their immunizing Antigens. The sera, all of which contained B17 activity, were produced in response to one of 10 <B>HLAB> Antigens (A2, Bw44, Bw49, Bw51, Bw55 , Bw56 , Bw57 , Bw58 , Bw62 and Bw63 ). Antisera stimulated By Bw57 and Bw58 cross-reacted with Bw49 and Both suBdivisions of B5 and B15, with Bidirectional cross-reactivity occurring in many instances. Bidirectional cross-reactivity was also oBserved Between Bw57 and A2 (an A2 stimulated antiserum also reacted with Bw58 ), and Bw57 and Bw55 . Immunization By Bw62 produced some antisera which showed strong cross-reactivity with Bw57 But no reactivity with Bw58 . Significant <B>HLAB>-B Antigen frequency disturBances were found in the responders to the B17 cross-reactive group Antigens. Twenty-five <B>HLAB> Antigens were found to comprise the B17 cross-reactive group and its related cross-reactions. The multideterminant nature of the <B>HLAB> Antigens is again emphasized By these findings.
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The <B>HLAB>-B12 and -B40 cross-reactive groups and their serological relationships.
Tissue Antigens, 2008Co-Authors: C. DarkeAbstract:: The serological analysis of 82 Broad <B>HLAB>-B antisera, produced By pregnancy alone, containing reactivity against <B>HLAB>-B12 and/or B40 positive cells and up to nine additional specificities was performed, using highly selected lymphocyte panels. The <B>HLAB> typing of 76 of the antiserum donors and 75 of their husBands showed that the antisera were stimulated in response to one of 10 different <B>HLAB>-B Antigens. It also showed the influence of serum donor <B>HLAB>-B Antigens on the reaction range of the antiserum produced, as well as significant <B>HLAB>-B Antigen frequency disturBances within various groups of the antiserum donors. Fifteen <B>HLAB>-B specificities were found to comprise the B12 cross-reactive group and its related cross-reactions, with Bidirectional cross-reactivity occurring Between Bw44, Bw45, Bw49 and Bw50 (unidirectional Between Bw44 and Bw50). Seventeen specificities were oBserved in the B40 cross-reactive group and its related cross-reactions, with Bidirectional cross-reactivity occurring Between Bw41, Bw50, Bw60 and Bw61. Antisera were studied that showed; (1) cross-reactivity Between Bw44, Bw45 and Bw60 stimulated antisera and Both suBdivisions of the B12 and B40 Antigens; (2) cross-reactivity Between B13, Bw44, Bw49, Bw60 and Bw61 stimulated antisera and Bw47; and (3) cross-reactivity Between Bw44, Bw49, Bw60 and Bw61 stimulated antisera and B13, with Bidirectional cross-reactivity occurring Between B13, Bw60 and Bw61 and Between B13 and Bw44. Bidirectional cross-reactivity was also oBserved Between B37 and Bw44 and Between B7 and Bw60. <B>HLAB>-Bw48 was shown to Be included within the reaction range of Both B7 and Bw60 stimulated antisera. Preferential cross-reactivity with one of two Antigen suBdivisions was extensively oBserved and occurred exclusively Between Antigens of the same Bw4/Bw6 association as the antiserum stimulating specificity. The results are discussed within the framework of the existence of multiple shared Antigenic determinants.
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The first example of an <B>HLAB>-Bw45 antiserum produced in a <B>HLAB>-Bw44 positive woman.
Tissue antigens, 2008Co-Authors: C. DarkeAbstract:An <B>HLAB>-Bw45 serum (Mu46), which was produced By pregnancy in a Bw44 positive woman, is descriBed. The serum was unaBle to Be aBsorBed By Bw44 positive cells. Blocking tests employing Broad B12 (Bw44 + Bw45) antisera, stimulated By either Bw44 or Bw45, showed that only F(aB)'2 from Bw45-stimulated B12 sera would completely Block the cytotoxicity of this serum. It was concluded that Bw44 and Bw45 are distinct specificities which share common determinants. These findings are Briefly discussed in relation to a recent report on the <B>HLAB>-B Antigen and supertypic Bw4, Bw6 Antigen relationship.
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Serological and molecular identification of an <B>HLAB>-B8 variant, <B>HLAB>-B8Jon (B*0802).
Experimental and Clinical Immunogenetics, 1996Co-Authors: C. Darke, Helen Fussell, J. Street, M. G. Guttridge, M. Thomas, Mark WorwoodAbstract:: The serological and molecular characteristics of the first <B>HLAB>-B8 variant (B8Jon), located on a haplotype Bearing <B>HLAB>-A1, -B8Jon, -Cw*07, DRB1*0301, DQA1*05, DQB1*0201, BfS, C4AQ0, C4B1 and the microsatellite alleles D6S265-3, 258-11, 105-8, 299-1 and 202-2 are identified and descriBed. This new <B>HLAB>-B Antigen was distinguished from the usual B8 By its lack of reactivity with Bw6 and most Bw4 antisera, together with its failure to react with most antisera cross-reactive with <B>HLAB>-B8. B8Jon gives a 'weaker' response than <B>HLAB>-B8 in titration studies with B8 antisera. It cannot Be differentiated from the usual B8 By one-dimensional iso-electric focusing. Time course studies, aBsorption analyses and serological tests on 17 Bw4 antiBodies suggest that B8Jon possesses an unusually 'weak' Bw4 epitope. Studies on the Bw4 sequence motif By PCR, using sequence-specific primers, indicate that B8Jon has a Bw4 sequence in common with other <B>HLAB>-B alleles, including B*4402-B*4405, rather than the Bw6 motif found on the familiar <B>HLAB>-B8 molecule.
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Serological identification of a new <B>HLAB>-B7 variant Antigen: <B>HLAB>-B7Qui
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics, 1994Co-Authors: H. Fussell, J. Street, M. Thomas, C. DarkeAbstract:SUMMARY A new <B>HLAB>-B Antigen, <B>HLAB>-B7Qui that appears to Be a variant of <B>HLAB>-B7 has Been identified. This Antigen, which is <B>HLAB>-Bw6 associated, reacts with approximately two-thirds of cytotoxic antisera stimulated By <B>HLAB>-B7 or B27 that lack a B27 or B7 component, respectively. All anti-B7+27 antisera (stimulated By either B7 or B27) react with B7Qui as do most B22-stimulated sera possessing a B7 component. However, sera stimulated By B60, with or without a B7 component, fail to react with B7Qui. Family studies show the B7Qui allele to Be unique to the haplotype –<B>HLAB>-A32 CW6 B7Qui Bf*S C4A*6 C4B*1 DR11 DQ7 (GL02). Six Caucasoid suBjects on the panel of 6861 <B>HLAB>-typed potential Bone marrow donors have this Antigen (phenotype frequency, 0.08745%; gene frequency, 0.04473%). Work undertaken during the 11th International HistocompatiBility Workshop (Reekers et al., 1992) showed that B7Qui has the same isoelectric point as <B>HLAB>-B702 and <B>HLAB>-B703 (Bpot) and that B7Qui is distinct from the <B>HLAB>-B7 variants B703, B7SL, BDT, B7x40, BRI, and B41v.
Bo Dupont - One of the best experts on this subject based on the ideXlab platform.
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Multiple transcripts of the killer cell immunogloBulin‐like receptor family, KIR3DLL (NKB1), are expressed By natural killer cells of a single individual
Tissue antigens, 1998Co-Authors: Y. Vyas, Annamalai Selvakumar, U. Steffens, Bo DupontAbstract:Natural killer (NK) cells express receptors that are ligands for <B>HLAB> class I molecules. One family of such NK receptors are called killer cell immunogloBulin-like receptors (KIR). The KIR2DL (inhiBiting) and KIR2DS (activating) molecules recognize <B>HLAB>-Cw Antigens, while the KIR3DL (inhiBiting) and KIR3DS (activating) molecules interact with <B>HLAB>-B Antigens with the Bw4 epitope. No NK receptors have yet Been identified for <B>HLAB>-B Antigens with the Bw6 epitope. We here report four novel full length cDNA transcripts encoding KIR3DL1-like proteins isolated from mRNA oBtained from interleukin-2-activated peripheral Blood mononuclear cells of a donor with two <B>HLAB>-B Antigens expressing the Bw6 epitope. These four transcripts Belong to a group of closely related KIR3DL1-like molecules initially defined By the cDNA clone NKB1. They differ from NKB1 By only 2 to 7 nucleotides and have 2 to 4 codon changes within the 423 residues of the mature protein. All transcripts were detected By RT-PCR, together with the previously reported KIR3DL1 transcripts, NKB1 and KIR3DL1v, in mRNA from NK cells of 10 of 10 donors tested, and in seven of eight NK clones derived from one donor. Functionally, the KIR3DL1 receptors expressed By five DX9-positive NK clones were not inhiBiting NK-mediated cytotoxicity when tested against the 721.221 B-lymphoBlastoid cell line expressing a <B>HLAB>-B Antigen with Bw4 epitope. All NK clones were, however, inhiBited By 721.221 cells transfected with a <B>HLAB>-B Antigen carrying the Bw6 epitope.
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Error rate for <B>HLAB>-B Antigen assignment By serology: implications for proficiency testing and utilization of DNA-Based typing methods.
Tissue antigens, 1997Co-Authors: M. V. Bozón, Julio C. Delgado, Annamalai Selvakumar, Olga P. Clavijo, Marcela Salazar, M. Ohashi, S. M. Alosco, J. Russell, Bo DupontAbstract:Until recently, the majority of <B>HLAB> class I typing has Been performed By serology. Expensive commercial typing trays are frequently used for testing non-Caucasian suBjects and new strategies using DNA-Based methods have Been adopted for improving clinical histocompatiBility testing results and adapted as supplements in proficiency testing. A douBle-Blind comparison of the typing of <B>HLAB>-B specificities in 40 samples was carried out Between serology and two polymerase chain reaction (PCR) methods, PCR amplification with sequence-specific primers (PCR-SSP) and PCR amplification and suBsequent hyBridization with sequence-specific oligonucleotide proBes (PCR-SSOP). The results demonstrated 22.5% misassignments of <B>HLAB>-B Antigens By serology. There was complete concordance Between the results oBtained with the two PCR Based typing methods. A second panel of 20 donor samples with incomplete or amBiguous serologic results was analyzed By PCR-SSP and SSOP Both PCR methods identified correctly the <B>HLAB>-B Antigens. Our results suggest that more accurate typing results can Be achieved By complementing serologic testing with DNA-Based typing techniques. The level of resolution for <B>HLAB>-B Antigen assignment can Be oBtained By this comBination of serology and limited DNA-Based typing is equivalent to the <B>HLAB>-B specificities defined By the WHO-<B>HLAB> Committee. This level of resolution cannot routinely Be achieved in clinical histocompatiBility testing or in proficiency testing using serologic reagents only.
Laura C. Bowman - One of the best experts on this subject based on the ideXlab platform.
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Effect of <B>HLAB> class I or class II incompatiBility in pediatric marrow transplantation from unrelated and related donors
Human immunology, 2001Co-Authors: Leung, Victoria Turner, Stacye Richardson, Ely Benaim, Gregory A. Hale, Edwin M. Horwitz, Paul Woodard, Laura C. BowmanAbstract:The degree of histoincompatiBility that can Be tolerated, and the relative importance of matching at individual <B>HLAB> class I and class II locus in Bone marrow transplantation (BMT) has not Been estaBlished. We hypothesized that matching for <B>HLAB>-DR may not Be more important than matching for <B>HLAB>-A or <B>HLAB>-B in selection of a donor for successful BMT. We retrospectively analyzed the outcomes of 248 consecutive pediatric patients who received allogeneic BMT from related donors (RD, n = 119) or unrelated donors (URD, n = 129). <B>HLAB>-A and <B>HLAB>-B were serologically matched, and <B>HLAB>-DRB1 were identical By DNA typing in 69% of donor-recipient pairs. Most patients (89%) had hematologic malignancies; the rest had aplastic anemia or a congenital disorder. One <B>HLAB>-A Antigen mismatch was associated with a decrease in survival (p = 0.003) and a delay in granulocyte engraftment (p = 0.02) in recipients of RD marrow; as well as a decrease in survival (p = 0.02) and the development of severe acute graft-versus-host disease (GVHD) (p = 0.03) in recipients of URD marrow. One <B>HLAB>-B Antigen mismatch was associated with a decrease in the survival (p = 0.05) and the development of severe GVHD (p = 0.0007) in recipients of RD marrow. One <B>HLAB>-DRB1 allele mismatch was associated only with a decrease in the survival (p = 0.0003) of recipients of RD marrow. Results of this study suggest that disparity in <B>HLAB>-A and <B>HLAB>-B Antigens may not Be Better tolerated than disparity in <B>HLAB>-DR allele in allogeneic BMT. Further studies are warranted to confirm our results.
Annamalai Selvakumar - One of the best experts on this subject based on the ideXlab platform.
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Multiple transcripts of the killer cell immunogloBulin‐like receptor family, KIR3DLL (NKB1), are expressed By natural killer cells of a single individual
Tissue antigens, 1998Co-Authors: Y. Vyas, Annamalai Selvakumar, U. Steffens, Bo DupontAbstract:Natural killer (NK) cells express receptors that are ligands for <B>HLAB> class I molecules. One family of such NK receptors are called killer cell immunogloBulin-like receptors (KIR). The KIR2DL (inhiBiting) and KIR2DS (activating) molecules recognize <B>HLAB>-Cw Antigens, while the KIR3DL (inhiBiting) and KIR3DS (activating) molecules interact with <B>HLAB>-B Antigens with the Bw4 epitope. No NK receptors have yet Been identified for <B>HLAB>-B Antigens with the Bw6 epitope. We here report four novel full length cDNA transcripts encoding KIR3DL1-like proteins isolated from mRNA oBtained from interleukin-2-activated peripheral Blood mononuclear cells of a donor with two <B>HLAB>-B Antigens expressing the Bw6 epitope. These four transcripts Belong to a group of closely related KIR3DL1-like molecules initially defined By the cDNA clone NKB1. They differ from NKB1 By only 2 to 7 nucleotides and have 2 to 4 codon changes within the 423 residues of the mature protein. All transcripts were detected By RT-PCR, together with the previously reported KIR3DL1 transcripts, NKB1 and KIR3DL1v, in mRNA from NK cells of 10 of 10 donors tested, and in seven of eight NK clones derived from one donor. Functionally, the KIR3DL1 receptors expressed By five DX9-positive NK clones were not inhiBiting NK-mediated cytotoxicity when tested against the 721.221 B-lymphoBlastoid cell line expressing a <B>HLAB>-B Antigen with Bw4 epitope. All NK clones were, however, inhiBited By 721.221 cells transfected with a <B>HLAB>-B Antigen carrying the Bw6 epitope.
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Error rate for <B>HLAB>-B Antigen assignment By serology: implications for proficiency testing and utilization of DNA-Based typing methods.
Tissue antigens, 1997Co-Authors: M. V. Bozón, Julio C. Delgado, Annamalai Selvakumar, Olga P. Clavijo, Marcela Salazar, M. Ohashi, S. M. Alosco, J. Russell, Bo DupontAbstract:Until recently, the majority of <B>HLAB> class I typing has Been performed By serology. Expensive commercial typing trays are frequently used for testing non-Caucasian suBjects and new strategies using DNA-Based methods have Been adopted for improving clinical histocompatiBility testing results and adapted as supplements in proficiency testing. A douBle-Blind comparison of the typing of <B>HLAB>-B specificities in 40 samples was carried out Between serology and two polymerase chain reaction (PCR) methods, PCR amplification with sequence-specific primers (PCR-SSP) and PCR amplification and suBsequent hyBridization with sequence-specific oligonucleotide proBes (PCR-SSOP). The results demonstrated 22.5% misassignments of <B>HLAB>-B Antigens By serology. There was complete concordance Between the results oBtained with the two PCR Based typing methods. A second panel of 20 donor samples with incomplete or amBiguous serologic results was analyzed By PCR-SSP and SSOP Both PCR methods identified correctly the <B>HLAB>-B Antigens. Our results suggest that more accurate typing results can Be achieved By complementing serologic testing with DNA-Based typing techniques. The level of resolution for <B>HLAB>-B Antigen assignment can Be oBtained By this comBination of serology and limited DNA-Based typing is equivalent to the <B>HLAB>-B specificities defined By the WHO-<B>HLAB> Committee. This level of resolution cannot routinely Be achieved in clinical histocompatiBility testing or in proficiency testing using serologic reagents only.
Hideyuki Kuwabara - One of the best experts on this subject based on the ideXlab platform.
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Drug-induced liver injury after allogeneic Bone marrow transplantation
International journal of hematology, 2013Co-Authors: Takayoshi Tachibana, Akito Nozaki, Makiko Enaka, Eri Yamamoto, Rika Kawasaki, Hideyuki Koharazawa, Maki Hagihara, Daisuke Ishibashi, Yuki Nakajima, Hideyuki KuwabaraAbstract:A 23-year-old woman developed acute severe hepatitis and jaundice on day 183 after Bone marrow transplantation from <B>HLAB>-B Antigen mismatched-related donor. The administration of prednisolone and cessation of the prescriBed drugs resolved the liver injury. Drug lymphocyte stimulation test was positive for acyclovir, and liver Biopsy indicated the characteristics of drug-induced liver injury (DILI) rather than graft-versus-host disease. Physicians should keep DILI in mind when considering differential diagnosis for liver complications after allogeneic cell transplantation.