The Experts below are selected from a list of 262770 Experts worldwide ranked by ideXlab platform
Xuemin Wang - One of the best experts on this subject based on the ideXlab platform.
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quantitative analysis of major plant Hormones in crude plant extracts by high performance liquid chromatography mass spectrometry
Nature Protocols, 2010Co-Authors: Xiangqing Pan, Ruth Welti, Xuemin WangAbstract:The ability to measure plant Hormones quantitatively is important as plant Hormones regulate plant growth, development and response to biotic and abiotic cues. In this protocol, we describe the quantitative analysis of major plant Hormones from crude plant extracts. Plant Hormones are determined using reverse-phase liquid chromatography-tandem mass spectrometry with multiple reaction monitoring. The method provides quantification of most major plant Hormones in a single run from 50 mg of fresh plant tissue. Extraction and quantitative analysis of 40 samples takes 2-3 d.
Xiangqing Pan - One of the best experts on this subject based on the ideXlab platform.
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quantitative analysis of major plant Hormones in crude plant extracts by high performance liquid chromatography mass spectrometry
Nature Protocols, 2010Co-Authors: Xiangqing Pan, Ruth Welti, Xuemin WangAbstract:The ability to measure plant Hormones quantitatively is important as plant Hormones regulate plant growth, development and response to biotic and abiotic cues. In this protocol, we describe the quantitative analysis of major plant Hormones from crude plant extracts. Plant Hormones are determined using reverse-phase liquid chromatography-tandem mass spectrometry with multiple reaction monitoring. The method provides quantification of most major plant Hormones in a single run from 50 mg of fresh plant tissue. Extraction and quantitative analysis of 40 samples takes 2-3 d.
William H. Catherino - One of the best experts on this subject based on the ideXlab platform.
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steroid Hormones and hormone antagonists regulate the neural marker neurotrimin in uterine leiomyoma
Fertility and Sterility, 2020Co-Authors: M Malik, Joy Britten, William H. Catherino, Toral Parikh, J M Aly, J PilgrimAbstract:Objective To characterize the role of steroid hormone and antihormone exposure on neurotrimin (NTM) expression in human leiomyoma and myometrial tissue and cells. Design Laboratory study of placebo and ulipristal acetate (UPA)–treated patient tissue. In vitro assessment of immortalized myometrial and leiomyoma cell lines after hormone and antihormone exposure. Setting Academic research center. Patient(s) Not applicable. Interventions(s) Exposure of leiomyoma cell lines to 17β-E2, medroxyprogesterone acetate (MPA), UPA, and fulvestrant. Main Outcome Measure(s) Messenger RNA expression quantified with the use of RNASeq analysis and quantitative real-time polymerase chain reaction (qRT-PCR). Protein levels quantified by means of Western blot analysis. Immunohistochemistry (IHC) on placebo- and UPA-treated patient uterine tissue specimens. Result(s) Expression of NTM in human uterine leiomyoma specimens according to RNASeq was increased compared with myometrium (5.22 ± 0.57–fold), which was confirmed with the use of qRT-PCR (1.95 ± 0.05). Furthermore, NTM protein was elevated in leiomyoma tissue compared with matched myometrium (2.799 ± 0.575). IHC revealed increased staining intensity in leiomyoma surgical specimens compared with matched myometrium of placebo patients. Western blot analysis in immortalized leiomyoma cell lines demonstrated an up-regulation of NTM protein expression (2.4 ± 0.04). Treatment of leiomyoma cell lines with 17β-E2 yielded a 1.98 ± 0.11–fold increase in NTM protein expression; however, treatment with fulvestrant showed no significant change compared with control. Leiomyoma cell lines demonstrated a 1.91 ± 0.97–fold increase in NTM protein expression after progesterone treatment. RNASeq analysis demonstrated a reduced expression in patient leiomyoma after UPA treatment (0.75 ± 0.14). Treatment of leiomyoma cells with UPA demonstrated a reduced total NTM protein amount (0.54 ± 0.31) in patients, which was confirmed with the use of IHC (UPA10 147.2 ± 9.40, UPA20 182.8 ± 8.98). In vitro studies with UPA treatment revealed a concentration-dependent effect that supported these findings. Conclusion(s) NTM, a neural cell adhesion molecule, is increased in leiomyoma compared with myometrium in patient tissue and in vitro models after estrogen and progesterone treatment. Down-regulation of expression occurs after UPA treatment, but not after fulvestrant exposure. Clinical Trial Registration Number NCT00290251 .
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gonadotropin releasing hormone analogues inhibit leiomyoma extracellular matrix despite presence of gonadal Hormones
Fertility and Sterility, 2016Co-Authors: M Malik, Joy Britten, William H. Catherino, A. PatelAbstract:Objective To determine the effect of GnRH analogues (GnRH-a) leuprolide acetate (LA) and cetrorelix acetate on gonadal hormone-regulated expression of extracellular matrix in uterine leiomyoma three-dimensional (3D) cultures. Design Laboratory study. Setting University research laboratory. Patient(s) Women undergoing hysterectomy for symptomatic leiomyomas. Intervention(s) The 3D cell cultures, protein analysis, Western blot, immunohistochemistry. Main Outcome Measure(s) Expression of extracellular matrix proteins, collagen 1, fibronectin, and versican in leiomyoma cells 3D cultures exposed to E 2 , P, LA, cetrorelix acetate, and combinations for 24- and 72-hour time points. Result(s) The 3D leiomyoma cultures exposed to E 2 for 24 hours demonstrated an increased expression of collagen-1 and fibronectin, which was maintained for up to 72 hours, a time point at which versican was up-regulated significantly. Although P up-regulated collagen-1 protein (1.29 ± 0.04) within 24 hours of exposure, significant increase in all extracellular matrix (ECM) proteins was observed when the gonadal Hormones were used concomitantly. Significant decrease in the amount of ECM proteins was observed on use of GnRH-a, LA and cetrorelix, with 24-hour exposure. Both the compounds also significantly decreased ECM protein concentration despite the presence of E 2 or both gonadal Hormones. Conclusion(s) This study demonstrates that GnRH-a directly affect the gonadal hormone-regulated collagen-1, fibronectin, and versican production in their presence. These findings suggest that localized therapy with GnRH-a may inhibit leiomyoma growth even in the presence of endogenous gonadal hormone exposure, thereby providing a mechanism to eliminate the hypoestrogenic side effects associated with GnRH-a therapy.
Carol A Derby - One of the best experts on this subject based on the ideXlab platform.
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sex Hormones in women with and without migraine evidence of migraine specific hormone profiles
Neurology, 2016Co-Authors: Jelena M Pavlovic, Amanda A Allshouse, Nanette Santoro, Sybil L Crawford, Rebecca C Thurston, Genevieve Nealperry, Richard B Lipton, Carol A DerbyAbstract:Objective: To compare daily sex hormone levels and rates of change between women with history of migraine and controls. Methods: History of migraine, daily headache diaries, and daily hormone data were collected in ovulatory cycles of pre- and early perimenopausal women in the Study of Women9s Health Across the Nation. Peak hormone levels, average daily levels, and within-woman day-to-day rates of decline over the 5 days following each hormone peak were calculated in ovulatory cycles for conjugated urinary estrogens (E1c), pregnanediol-3-glucuronide, luteinizing hormone, and follicle-stimulating hormone. Comparisons were made between migraineurs and controls using 2-sample t tests on the log scale with results reported as geometric means. Results: The sample included 114 women with history of migraine and 223 controls. Analyses of within-woman rates of decline showed that E1c decline over the 2 days following the luteal peak was greater in migraineurs for both absolute rate of decline (33.8 [95% confidence interval 28.0–40.8] pg/mgCr vs 23.1 [95% confidence interval 20.1–26.6] pg/mgCr, p = 0.002) and percent change (40% vs 30%, p Conclusions: Migraineurs are characterized by faster late luteal phase E1c decline compared to controls. The timing and rate of estrogen withdrawal before menses may be a marker of neuroendocrine vulnerability in women with migraine.
Helen L. Henry - One of the best experts on this subject based on the ideXlab platform.
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The Hypothalamus and Anterior Pituitary
Hormones, 2015Co-Authors: Anthony W. Norman, Helen L. HenryAbstract:The pituitary gland has often been called “the master gland” because so many important functions are governed by the Hormones it secretes. In this chapter readers see that the pituitary Hormones are under the control of both the central nervous system, especially the hypothalamus and the secretions of the distal endocrine glands, and other internal signals. The hypothalamic releasing Hormones function to control the secretions of the anterior pituitary Hormones, either positively or negatively. The Hormones secreted by the distal endocrine glands exert, in general, negative feedback effects in the brain and in the anterior pituitary. There are many signals originating either outside or inside the body that are mediated by the central nervous system. Thus, changes in the environment can ultimately stimulate the secretion of hypothalamic releasing Hormones, which, through their effects on pituitary hormonal secretion allow the body to adapt to the change. Likewise, signals from within the organism can trigger this cascade system so that secretion of Hormones from distant target glands can be affected. The chapter covers control cascades; anatomical relationships; structure, synthesis, secretion, and target cells of the hypothalamic releasing Hormones; chemistry of the anterior pituitary Hormones; regulation and biological actions of growth hormone and prolactin; involvement of the hypothalamus in appetite regulation; and clinical aspects of the hypothalamus and pituitary.
