The Experts below are selected from a list of 2256 Experts worldwide ranked by ideXlab platform
Benham E Lockhart - One of the best experts on this subject based on the ideXlab platform.
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Characterization of a Potexvirus infecting Hosta spp.
Plant Disease, 1996Co-Authors: Sophie Currier, Benham E LockhartAbstract:A previously undescribed potexvirus, named Hosta virus X (HVX), was found in 17 naturally infected Hosta (Hosta spp.) cultivars from Minnesota, Indiana, Illinois, Iowa, and Michigan. HVX was readily transmitted mechanically but infected only Nicotiana benthamiana and Hosta spp., in which symptoms ranged from severe mosaic and leaf necrosis to latency. Particles of HVX averaged 530 nm in length, had a buoyant density in cesium chloride of 1.28 gm/cm 3 , and contained a single genomic species of ssRNA approximately 3 kb in size. The capsid protein of HVX had a molecular mass of approximately 27 kDa. In indirect enzyme immunoassays, HVX reacted with an antiserum to clover yellow mosaic virus (C1YMV), and less strongly with antiserum to hydrangea ringspot virus (HRSV), but not with antisera to any of 14 other potexviruses tested. However, in reciprocal tests, CIYMV reacted only very weakly with HVX antiserum. HVX did not infect any of three cultivars of pea that were susceptible to infection by CIYMV, and CIYMV did not infect any of three Hosta cultivars susceptible to infection by HVX. Spread of HVX infection in Hosta appears to occur by vegetative propagation and accidental mechanical transmission, and management of the disease can be achieved by virus indexing and cultural practices that minimize the risk of virus spread to susceptible cultivars.
Masayuki Maki - One of the best experts on this subject based on the ideXlab platform.
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Isolation and characterization of microsatellite markers from the Hosta species Hosta albomarginata (Liliaceae)
Molecular ecology resources, 2008Co-Authors: Hidetoshi Yamamoto, Takayuki Yamada, Masayuki MakiAbstract:Hostas are very popular ornamental plants in gardens in the United States, Europe, and East Asia. We have developed nine microsatellite loci from an enrichment library of genomic DNA in Hosta albomarginata. We characterized these nine microsatellite loci for 20 individuals from a population of H. albomarginata. The primers developed in this study yielded an average of 12.4 alleles per locus (range five to 20) and an average expected heterozygosity of 0.86 (range 0.65 to 0.95). These markers will be powerful tools for breeding programmes of Hosta cultivars, studies of population genetics, and the conservation of wild Hosta species.
Dennis J. Lewandowski - One of the best experts on this subject based on the ideXlab platform.
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Biological and Molecular Characterization of a U.S. Isolate of Hosta virus X
Phytopathology, 2012Co-Authors: Carola M. De La Torre, Margaret G. Redinbaugh, Dennis J. LewandowskiAbstract:ABSTRACT Hosta virus X (HVX) is rapidly becoming a serious pathogen of commercially important Hosta plants worldwide. We report here biological and molecular characterization of a U.S. isolate of HVX, HVX-37. HVX-37 infectivity was tested in 21 Hosta cultivars over three growth seasons, and three types of responses were defined based upon the ability of the virus to cause local and/or systemic infections. Four cultivars resistant to systemic HVX infection were identified. The full-length sequence of the HVX-37 genome was determined, the first complete sequence of a U.S. HVX isolate. Comparison with the previously sequenced HVX-Korea (Kr) genome revealed a high level of sequence similarity, as well as some differences. Notably, a 105-nucleotide long, near-perfect direct repeat in the Kr isolate is absent in HVX-37. The accuracy of the HVX-37 genome sequence was confirmed by infectivity of in vitro transcripts synthesized from a full-length HVX-37 cDNA on Nicotiana benthamiana and Hosta plants.
Sophie Currier - One of the best experts on this subject based on the ideXlab platform.
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Characterization of a Potexvirus infecting Hosta spp.
Plant Disease, 1996Co-Authors: Sophie Currier, Benham E LockhartAbstract:A previously undescribed potexvirus, named Hosta virus X (HVX), was found in 17 naturally infected Hosta (Hosta spp.) cultivars from Minnesota, Indiana, Illinois, Iowa, and Michigan. HVX was readily transmitted mechanically but infected only Nicotiana benthamiana and Hosta spp., in which symptoms ranged from severe mosaic and leaf necrosis to latency. Particles of HVX averaged 530 nm in length, had a buoyant density in cesium chloride of 1.28 gm/cm 3 , and contained a single genomic species of ssRNA approximately 3 kb in size. The capsid protein of HVX had a molecular mass of approximately 27 kDa. In indirect enzyme immunoassays, HVX reacted with an antiserum to clover yellow mosaic virus (C1YMV), and less strongly with antiserum to hydrangea ringspot virus (HRSV), but not with antisera to any of 14 other potexviruses tested. However, in reciprocal tests, CIYMV reacted only very weakly with HVX antiserum. HVX did not infect any of three cultivars of pea that were susceptible to infection by CIYMV, and CIYMV did not infect any of three Hosta cultivars susceptible to infection by HVX. Spread of HVX infection in Hosta appears to occur by vegetative propagation and accidental mechanical transmission, and management of the disease can be achieved by virus indexing and cultural practices that minimize the risk of virus spread to susceptible cultivars.
Y. J. Zhang - One of the best experts on this subject based on the ideXlab platform.
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First Report of Hosta virus X Infecting Hosta Plants in China.
Plant disease, 2013Co-Authors: M. S. Wei, Y. J. ZhangAbstract:Hosta (Hosta spp.) plants showing leaf deformation, puckering, and ink-bleed symptoms were collected in July 2012 from a park at Dongcheng district, Beijing, China. Three out of six samples tested positive for Hosta virus X (HVX) by immunostrip and double-antibody sandwich (DAS)-ELISA with HVX-specific serological reagents from Agdia Inc. (Elkhart, IN, USA). Filamentous viral particles were trapped and observed from the infected Hosta leaf sap by immuno-serological electron microscopy (ISEM) (antibodies from Agdia). To confirm HVX infection, three ELISA-positive samples were analyzed by reverse transcription-PCR assay, using virus-specific primers HVXf (5′-ATCCGTTATCTACAGGGGACCAG-3′) and HVXr (5′-TAAGTTAGTGGAACGGTTAGCCCGAT-3′) that amplified a 1,067-bp fragment including the coat protein (CP) coding region. The CP nucleotide sequence comparisons showed 99% to 100% homology among the three isolates named HVXBJ4, HVXBJ5, and HVXBJ6 (GenBank Accession No. JX535292, JX535293, and JX535294) and with the HVX se...