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Chuanlun Zhang - One of the best experts on this subject based on the ideXlab platform.

  • Diversity of crenarchaeota in terrestrial Hot Springs and their surrounding environments in Kamchatka, Russia
    Wei sheng wu xue bao = Acta microbiologica Sinica, 2013
    Co-Authors: Zhao-qi Song, Jinquan Chen, En-min Zhou, Li Wang, Chuanlun Zhang
    Abstract:

    Crenarchaeota is a major archaeal lineage in terrestrial Hot Springs and important in biogeochemical cycles of life-essential elements. In this study, we investigated the diversity of Crenarchaeota in Hot Springs and the surrounding environments in Kamchatka, Russia. In addition, we compared crenarchaeotal community structures in Kamchatka, Russia and Yunnan province, China. Crenarchaeal 16S rRNA gene clone libraries were constructed and the sequences and abundances of representational clone were obtained. Phylogenetic analysis was then performed and the community structures in different samples were compared. The high temperature spring Burlyashi Liza (BSL, 89 degrees C) comprised Thermoprotei. The moderate temperature spring TF Vent 2 (TFV, 49 degrees C) harbored unidentified Thermoprotei group, unidentified crenarchaeal group, HWCG-II (Hot water crenarchaeotal group II), and Group1. 1b (one thaumarchaeotal subgroup). Most of sequences that obtained from surrounding environments ( <15 degrees C) are closely with the representational clone pJP from a Yellowstone Hot spring. Jackknife cluster and Principal coordinates analysis (PCoA) showed that the samples have more similarity in crenarchaeal communities at similar temperatures. The diversities of Crenarchaeota in Kamchatka Hot Springs are somewhat different from those in Yunnan province. Terrestrial Hot Springs obviously affect the crenarchaeotal communities in surrounding environments. Temperature is the major factor controlling the community structure in terrestrial Hot Springs.

  • diversity of crenarchaeota in terrestrial Hot Springs and their surrounding environments in kamchatka russia
    Acta Microbiologica Sinica, 2013
    Co-Authors: Zhao-qi Song, Jinquan Chen, En-min Zhou, Li Wang, Chuanlun Zhang
    Abstract:

    OBJECTIVE: Crenarchaeota is a major archaeal lineage in terrestrial Hot Springs and important in biogeochemical cycles of life-essential elements. In this study, we investigated the diversity of Crenarchaeota in Hot Springs and the surrounding environments in Kamchatka, Russia. In addition, we compared crenarchaeotal community structures in Kamchatka, Russia and Yunnan province, China. METHODS: Crenarchaeal 16S rRNA gene clone libraries were constructed and the sequences and abundances of representational clone were obtained. Phylogenetic analysis was then performed and the community structures in different samples were compared. RESULTS: The high temperature spring Burlyashi Liza (BSL, 89 degrees C) comprised Thermoprotei. The moderate temperature spring TF Vent 2 (TFV, 49 degrees C) harbored unidentified Thermoprotei group, unidentified crenarchaeal group, HWCG-II (Hot water crenarchaeotal group II), and Group1. 1b (one thaumarchaeotal subgroup). Most of sequences that obtained from surrounding environments ( <15 degrees C) are closely with the representational clone pJP from a Yellowstone Hot spring. Jackknife cluster and Principal coordinates analysis (PCoA) showed that the samples have more similarity in crenarchaeal communities at similar temperatures. CONCLUSION: The diversities of Crenarchaeota in Kamchatka Hot Springs are somewhat different from those in Yunnan province. Terrestrial Hot Springs obviously affect the crenarchaeotal communities in surrounding environments. Temperature is the major factor controlling the community structure in terrestrial Hot Springs.

  • control of temperature on microbial community structure in Hot Springs of the tibetan plateau
    PLOS ONE, 2013
    Co-Authors: Shang Wang, Hongchen Jiang, Zhao-qi Song, Chuanlun Zhang, Hailiang Dong, Weiguo Hou, Liuqin Huang, Yong Zhang
    Abstract:

    The Tibetan Plateau in Northwest China hosts a number of Hot Springs that represent a biodiversity Hotspot for thermophiles, yet their diversity and relationship to environmental conditions are poorly explored in these habitats. In this study we investigated microbial diversity and community composition in 13 Tibetan Hot Springs with a wide range of temperatures (22.1–75uC) and other geochemical conditions by using the 16S rRNA gene pyrosequencing approach. Bacteria (10 8 –10 11 copy/g; 42 bacterial phyla) in Tibetan Hot Springs were more abundant and far more diverse than Archaea (10 7 –10 10 copy/g; 5 archaeal phyla). The dominant bacterial phyla systematically varied with temperature. Moderate temperatures (75–66uC) favored Aquificae, GAL35, and novel Bacteria, whereas low temperatures (60–22.1uC) selected for Deinococcus-Thermus, Cyanobacteria, and Chloroflexi. The relative abundance of Aquificae was correlated positively with temperature, but the abundances of Deinococcus-Thermus, Cyanobacteria, and Chloroflexi were negatively correlated with temperature. Cyanobacteria and Chloroflexi were abundant in Tibetan Hot Springs and their abundances were positively correlated at low temperatures (55–43uC) but negatively correlated at moderate temperatures (75–55uC). These correlation patterns suggest a complex physiological relationship between these two phyla. Most archaeal sequences were related to Crenarchaeota with only a few related to Euryarchaeota and Thaumarchaeota. Despite the fact that microbial composition in Tibetan Hot Springs was strongly shaped by temperature, microbial diversity (richness, evenness and Shannon diversity) was not significantly correlated with temperature change. The results of this study expand our current understanding of microbial ecology in Tibetan Hot Springs and provide a basis for a global comparison.

  • Bacterial and archaeal diversities in Yunnan and Tibetan Hot Springs, China.
    Environmental microbiology, 2012
    Co-Authors: Zhao-qi Song, Hongchen Jiang, Xiaoyang Zhi, Fengping Wang, Jinquan Chen, En-min Zhou, Feng Liang, Xiang Xiao, Shu-kun Tang, Chuanlun Zhang
    Abstract:

    Summary Thousands of Hot Springs are located in the north-eastern part of the Yunnan–Tibet geothermal zone, which is one of the most active geothermal areas in the world. However, a comprehensive and detailed understanding of microbial diversity in these Hot Springs is still lacking. In this study, bacterial and archaeal diversities were investigated in 16 Hot Springs (pH 3.2–8.6; temperature 47–96°C) in Yunnan Province and Tibet, China by using a barcoded 16S rRNA gene-pyrosequencing approach. Aquificae, Proteobacteria, Firmicutes, Deinococcus-Thermus and Bacteroidetes comprised the large portion of the bacterial communities in acidic Hot Springs. Non-acidic Hot Springs harboured more and variable bacterial phyla than acidic Springs. Desulfurococcales and unclassified Crenarchaeota were the dominated groups in archaeal populations from most of the non-acidic Hot Springs; whereas, the archaeal community structure in acidic Hot Springs was simpler and characterized by Sulfolobales and Thermoplasmata. The phylogenetic analyses showed that Aquificae and Crenarchaeota were predominant in the investigated Springs and possessed many phylogenetic lineages that have never been detected in other Hot Springs in the world. Thus findings from this study significantly improve our understanding of microbial diversity in terrestrial Hot Springs.

  • Distribution of glycerol dialkyl glycerol tetraethers in Tibetan Hot Springs
    Geoscience Frontiers, 2012
    Co-Authors: Chuanlun Zhang, Hailiang Dong, Bin Fang, Genhou Wang
    Abstract:

    Abstract Isoprenoidal glycerol dialkyl glycerol tetraethers (iGDGTs) from the Gulu Hot Springs (23–83.6 °C, pH > 7) and Yangbajing Hot Springs (80–128 °C, pH > 7) were analyzed in order to investigate the distribution of archaeal lipids among different Hot Springs in Tibet. A soil sample from Gulu was incubated at different temperatures and analyzed for changes in iGDGTs to help evaluate whether surrounding soil may contribute to the iGDGTs in Hot Springs. The sources of bacterial GDGTs (bGDGTs) in these Hot Springs were also investigated. The results revealed different profiles of iGDGTs between Gulu and Yangbajing Hot Springs. Core iGDGTs and polar iGDGTs also presented different patterns in each Hot spring. The PCA analysis showed that the structure of polar iGDGTs can be explained by three factors and suggested multiple sources of these compounds. Bivariate correlation analysis showed significant positive correlations between polar and core bGDGTs, suggesting the in situ production of bGDGTs in the Hot Springs. Furthermore, in the soil incubation experiment, temperature had the most significant influence on concentration of bGDGTs rather than iGDGTs, and polar bGDGTs had greater variability than core bGDGTs with changing temperature. Our results indicated that soil input had little influence on the composition of GDGTs in Tibetan Hot Springs. On the other hand, ring index and TEX86 values were both positively correlated with incubation temperature, suggesting that the structure of archaeal lipids changed in response to varying temperature during incubation.

Hailiang Dong - One of the best experts on this subject based on the ideXlab platform.

  • reduction of structural fe iii in nontronite by thermophilic microbial consortia enriched from Hot Springs in tengchong yunnan province china
    Chemical Geology, 2017
    Co-Authors: Hongchen Jiang, Hailiang Dong, Liuqin Huang, Xi Wang, Can Feng, Zizhang Liu, Qiang Zeng, Li Zhang
    Abstract:

    Abstract Iron redox cycling provides one of the most important energy sources supporting microorganisms in terrestrial Hot Springs. In order to understand microbial Fe(III) reduction processes in Hot Springs, Fe(III)-reducing bacterial consortia were successfully retrieved from seven acidic Hot Springs in Tengchong County, Yunnan Province, China (a temperature range from 40.6 °C to 74.7 °C and a pH range from 2.64 to 4.72). These Fe(III)-reducing consortia were mainly composed of sulfate reducing bacteria (SRB, including Desulfotomaculum, Thermodesulfobacterium and Desulfomicrobium) and organic matter fermenting bacteria (Thermoanaerobacterium and Thermoanaerobacter), with each consortia having specific composition depending on the physio-chemical conditions of the studied Hot Springs. The obtained consortia were capable of dissimilatory reduction of Fe(III) in nontronite NAu-2 (ferruginous smectite) with 20.8% to 29.8% reduction extent and initial reduction rate of 0.011–0.023 mM Fe(III)/g NAu-2/h. These extents and rates were comparable to those by mesophilic Fe-reducers in the presence of electron shuttles. Both high temperature and the possible presence of electron-shuttles in Hot Springs may have accounted for these observed high reduction extents and rates. Various secondary minerals formed during NAu-2 bioreduction, including Fe sulfides and oxides, silicates, and silica. In one spring, illitization and kaolinization of smectite were observed, suggesting that Fe-reducers play important roles in mineral transformation in geothermal environments.

  • archaeal and bacterial diversity in acidic to circumneutral Hot Springs in the philippines
    FEMS Microbiology Ecology, 2013
    Co-Authors: Qiuyuan Huang, Hongchen Jiang, Shang Wang, Weiguo Hou, Brandon R Briggs, Ramonito Solis, Carlo A Arcilla, Teofilo Abrajano, Hailiang Dong
    Abstract:

    The microbial diversity was investigated in sediments of six acidic to circumneutral Hot Springs (Temperature: 60–92 °C, pH 3.72–6.58) in the Philippines using an integrated approach that included geochemistry and 16S rRNA gene pyrosequencing. Both bacterial and archaeal abundances were lower in high-temperature Springs than in moderate-temperature ones. Overall, the archaeal community consisted of sequence reads that exhibited a high similarity (nucleotide identity > 92%) to phyla Crenarchaeota, Euryarchaeota, and unclassified Archaea. The bacterial community was composed of sequence reads moderately related (nucleotide identity > 90%) to 17 phyla, with Aquificae and Firmicutes being dominant. These phylogenetic groups were correlated with environmental conditions such as temperature, dissolved sulfate and calcium concentrations in spring water, and sediment properties including total nitrogen, pyrite, and elemental sulfur. Based on the phylogenetic inference, sulfur metabolisms appear to be key physiological functions in these Hot Springs. Sulfobacillus (within phylum Firmicutes) along with members within Sulfolobales were abundant in two high-temperature Springs (> 76 °C), and they were hypothesized to play an important role in regulating the sulfur cycling under high-temperature conditions. The results of this study improve our understanding of microbial diversity and community composition in acidic to circumneutral terrestrial Hot Springs and their relationships with geochemical conditions.

  • control of temperature on microbial community structure in Hot Springs of the tibetan plateau
    PLOS ONE, 2013
    Co-Authors: Shang Wang, Hongchen Jiang, Zhao-qi Song, Chuanlun Zhang, Hailiang Dong, Weiguo Hou, Liuqin Huang, Yong Zhang
    Abstract:

    The Tibetan Plateau in Northwest China hosts a number of Hot Springs that represent a biodiversity Hotspot for thermophiles, yet their diversity and relationship to environmental conditions are poorly explored in these habitats. In this study we investigated microbial diversity and community composition in 13 Tibetan Hot Springs with a wide range of temperatures (22.1–75uC) and other geochemical conditions by using the 16S rRNA gene pyrosequencing approach. Bacteria (10 8 –10 11 copy/g; 42 bacterial phyla) in Tibetan Hot Springs were more abundant and far more diverse than Archaea (10 7 –10 10 copy/g; 5 archaeal phyla). The dominant bacterial phyla systematically varied with temperature. Moderate temperatures (75–66uC) favored Aquificae, GAL35, and novel Bacteria, whereas low temperatures (60–22.1uC) selected for Deinococcus-Thermus, Cyanobacteria, and Chloroflexi. The relative abundance of Aquificae was correlated positively with temperature, but the abundances of Deinococcus-Thermus, Cyanobacteria, and Chloroflexi were negatively correlated with temperature. Cyanobacteria and Chloroflexi were abundant in Tibetan Hot Springs and their abundances were positively correlated at low temperatures (55–43uC) but negatively correlated at moderate temperatures (75–55uC). These correlation patterns suggest a complex physiological relationship between these two phyla. Most archaeal sequences were related to Crenarchaeota with only a few related to Euryarchaeota and Thaumarchaeota. Despite the fact that microbial composition in Tibetan Hot Springs was strongly shaped by temperature, microbial diversity (richness, evenness and Shannon diversity) was not significantly correlated with temperature change. The results of this study expand our current understanding of microbial ecology in Tibetan Hot Springs and provide a basis for a global comparison.

  • Distribution of glycerol dialkyl glycerol tetraethers in Tibetan Hot Springs
    Geoscience Frontiers, 2012
    Co-Authors: Chuanlun Zhang, Hailiang Dong, Bin Fang, Genhou Wang
    Abstract:

    Abstract Isoprenoidal glycerol dialkyl glycerol tetraethers (iGDGTs) from the Gulu Hot Springs (23–83.6 °C, pH > 7) and Yangbajing Hot Springs (80–128 °C, pH > 7) were analyzed in order to investigate the distribution of archaeal lipids among different Hot Springs in Tibet. A soil sample from Gulu was incubated at different temperatures and analyzed for changes in iGDGTs to help evaluate whether surrounding soil may contribute to the iGDGTs in Hot Springs. The sources of bacterial GDGTs (bGDGTs) in these Hot Springs were also investigated. The results revealed different profiles of iGDGTs between Gulu and Yangbajing Hot Springs. Core iGDGTs and polar iGDGTs also presented different patterns in each Hot spring. The PCA analysis showed that the structure of polar iGDGTs can be explained by three factors and suggested multiple sources of these compounds. Bivariate correlation analysis showed significant positive correlations between polar and core bGDGTs, suggesting the in situ production of bGDGTs in the Hot Springs. Furthermore, in the soil incubation experiment, temperature had the most significant influence on concentration of bGDGTs rather than iGDGTs, and polar bGDGTs had greater variability than core bGDGTs with changing temperature. Our results indicated that soil input had little influence on the composition of GDGTs in Tibetan Hot Springs. On the other hand, ring index and TEX86 values were both positively correlated with incubation temperature, suggesting that the structure of archaeal lipids changed in response to varying temperature during incubation.

Elizaveta Bonch-osmolovskaya - One of the best experts on this subject based on the ideXlab platform.

  • Anaerobic transformation of carbon monoxide by microbial communities of Kamchatka Hot Springs
    Extremophiles, 2011
    Co-Authors: Tatiana V. Kochetkova, Aleksander V Lebedinsky, T V Kolganova, Elizaveta Bonch-osmolovskaya, Igor I Rusanov, Nikolay V. Pimenov, Tatyana G. Sokolova
    Abstract:

    Carbon monoxide (CO) is one of the common gaseous compounds found in Hot volcanic environments. It is known to serve as the growth substrate for a number of thermophilic prokaryotes, both aerobic and anaerobic. The goal of this work was to study the process of anaerobic transformation of CO by microbial communities inhabiting natural thermal environments: Hot Springs of Uzon Caldera, Kamchatka. The anaerobic microbial community of Treshchinny Spring (80°C, pH 6.5) was found to exhibit two peaks of affinity for CO ( K _S1 = 54 nM and K _S2 = 1 μM). The actual rate of anaerobic CO transformation by the microbial community of this spring, calculated after obtaining the concentration dependence curve and extrapolated to the natural concentration of CO dissolved in the Hot spring water (20 nM), was found to be 120 μmol l^−1 of sediment day^−1. In all the Hot Springs studied, more than 90% of the carbon of ^14CO upon anaerobic incubation was recovered as ^14CO_2. From 1 to 5% of ^14CO was transformed to volatile fatty acids (VFA). The number of microorganisms capable of anaerobic CO oxidation determined by dilution-to-extinction method reached 10^6 cells ml^−1 of sediment. CO-transforming anaerobic thermophilic microorganisms isolated from the Springs under study exhibited hydrogenogenic type of CO oxidation and belonged to the bacterial genera Carboxydocella and Dictyoglomus. These data suggest a significant role of hydrogenogenic carboxydotrophic prokaryotes in anaerobic CO transformation in Uzon Caldera Hot Springs.

  • Biodiversity of Thermophilic Prokaryotes with Hydrolytic Activities in Hot Springs of Uzon Caldera, Kamchatka (Russia)
    Applied and Environmental Microbiology, 2009
    Co-Authors: Ilya V Kublanov, Anna A Perevalova, G B Slobodkina, Aleksander V Lebedinsky, Salima Kh Bidzhieva, T V Kolganova, E N Kaliberda, L D Rumsh, Thomas Haertle, Elizaveta Bonch-osmolovskaya
    Abstract:

    Samples of water from the Hot Springs of Uzon Caldera with temperatures from 68 to 87 C and pHs of 4.1 to 7.0, supplemented with proteinaceous (albumin, casein, or alpha- or beta-keratin) or carbohydrate (cellulose, carboxymethyl cellulose, chitin, or agarose) biological polymers, were filled with thermal water and incubated at the same sites, with the contents of the tubes freely accessible to the hydrothermal fluid. As a result, several enrichment cultures growing in situ on different polymeric substrates were obtained. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene fragments obtained after PCR with Bacteria-specific primers showed that the bacterial communities developing on carbohydrates included the genera Caldicellulosiruptor and Dictyoglomus and that those developing on proteins contained members of the Thermotogales order. DGGE analysis performed after PCR with Archaea- and Crenarchaeota-specific primers showed that archaea related to uncultured environmental clones, particularly those of the Crenarchaeota phylum, were present in both carbohydrate- and protein-degrading communities. Five isolates obtained from in situ enrichments or corresponding natural samples of water and sediments represented the bacterial genera Dictyoglomus and Caldanaerobacter as well as new archaea of the Crenarchaeota phylum. Thus, in situ enrichment and consequent isolation showed the diversity of thermophilic prokaryotes competing for biopolymers in microbial communities of terrestrial Hot Springs.

  • Biodiversity of thermophilic prokaryotes with hydrolytic activities in Hot Springs of Uzon Caldera, Kamchatka (Russia).
    Applied and environmental microbiology, 2008
    Co-Authors: Ilya V Kublanov, Anna A Perevalova, G B Slobodkina, Aleksander V Lebedinsky, Salima Kh Bidzhieva, T V Kolganova, E N Kaliberda, L D Rumsh, Thomas Haertle, Elizaveta Bonch-osmolovskaya
    Abstract:

    Samples of water from the Hot Springs of Uzon Caldera with temperatures from 68 to 87 degrees C and pHs of 4.1 to 7.0, supplemented with proteinaceous (albumin, casein, or alpha- or beta-keratin) or carbohydrate (cellulose, carboxymethyl cellulose, chitin, or agarose) biological polymers, were filled with thermal water and incubated at the same sites, with the contents of the tubes freely accessible to the hydrothermal fluid. As a result, several enrichment cultures growing in situ on different polymeric substrates were obtained. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene fragments obtained after PCR with Bacteria-specific primers showed that the bacterial communities developing on carbohydrates included the genera Caldicellulosiruptor and Dictyoglomus and that those developing on proteins contained members of the Thermotogales order. DGGE analysis performed after PCR with Archaea- and Crenarchaeota-specific primers showed that archaea related to uncultured environmental clones, particularly those of the Crenarchaeota phylum, were present in both carbohydrate- and protein-degrading communities. Five isolates obtained from in situ enrichments or corresponding natural samples of water and sediments represented the bacterial genera Dictyoglomus and Caldanaerobacter as well as new archaea of the Crenarchaeota phylum. Thus, in situ enrichment and consequent isolation showed the diversity of thermophilic prokaryotes competing for biopolymers in microbial communities of terrestrial Hot Springs.

  • Distribution of Crenarchaeota representatives in terrestrial Hot Springs of Russia and Iceland.
    Applied and environmental microbiology, 2008
    Co-Authors: Anna A Perevalova, Christa Schleper, T V Kolganova, Elizaveta Bonch-osmolovskaya, Nils-kåre Birkeland, Aleksander V Lebedinsky
    Abstract:

    Culture-independent (PCR with Crenarchaeota-specific primers and subsequent denaturing gradient gel electrophoresis) and culture-dependent approaches were used to study the diversity of Crenarchaeota in terrestrial Hot Springs of the Kamchatka Peninsula and the Lake Baikal region (Russia) and of Iceland. Among the phylotypes detected there were relatives of both cultured (mainly hyperthermophilic) and uncultured Crenarchaeota. It was found that there is a large and diverse group of uncultured Crenarchaeota that inhabit terrestrial Hot Springs with moderate temperatures (55 to 70°C). Two of the lineages of this group were given phenotypic characterization, one as a result of cultivation in an enrichment culture and another one after isolation of a pure culture, “Fervidococcus fontis,” which proved to be a moderately thermophilic, neutrophilic (optimum pH of 6.0 to 7.5), anaerobic organotroph.

Anna A Perevalova - One of the best experts on this subject based on the ideXlab platform.

  • microbial diversity and autotrophic activity in kamchatka Hot Springs
    Extremophiles, 2017
    Co-Authors: Alexander Y Merkel, Anna A Perevalova, G B Slobodkina, Nikolay Pimenov, Igor I Rusanov, A I Slobodkin, Ivan Yu Tarnovetckii, Evgeny N Frolov, Arseny V Dubin, Elizaveta A Bonchosmolovskaya
    Abstract:

    Microbial communities of Kamchatka Peninsula terrestrial Hot Springs were studied using molecular, radioisotopic and cultural approaches. Analysis of 16S rRNA gene fragments performed by means of high-throughput sequencing revealed that aerobic autotrophic sulfur-oxidizing bacteria of the genus Sulfurihydrogenibium (phylum Aquificae) dominated in a majority of streamers. Another widely distributed and abundant group was that of anaerobic bacteria of the genus Caldimicrobium (phylum Thermodesulfobacteria). Archaea of the genus Vulcanisaeta were abundant in a high-temperature, slightly acidic Hot spring, where they were accompanied by numerous Nanoarchaeota, while the domination of uncultured Thermoplasmataceae A10 was characteristic for moderately thermophilic acidic habitats. The highest rates of inorganic carbon assimilation determined by the in situ incubation of samples in the presence of 14C-labeled bicarbonate were found in oxygen-dependent streamers; in two sediment samples taken from the Hottest Springs this process, though much weaker, was found to be not dependent on oxygen. The isolation of anaerobic lithoautotrophic prokaryotes from Kamchatka Hot Springs revealed a wide distribution of the ability for sulfur disproportionation, a new lithoautotrophic process capable to fuel autonomous anaerobic ecosystems.

  • biodiversity of thermophilic prokaryotes with hydrolytic activities in Hot Springs of uzon caldera kamchatka russia
    Applied and Environmental Microbiology, 2009
    Co-Authors: Ilya V Kublanov, Anna A Perevalova, G B Slobodkina, Aleksander V Lebedinsky, Salima Kh Bidzhieva, T V Kolganova, E N Kaliberda, L D Rumsh, Thomas Haertle, Elizaveta A Bonchosmolovskaya
    Abstract:

    Samples of water from the Hot Springs of Uzon Caldera with temperatures from 68 to 87°C and pHs of 4.1 to 7.0, supplemented with proteinaceous (albumin, casein, or α- or β-keratin) or carbohydrate (cellulose, carboxymethyl cellulose, chitin, or agarose) biological polymers, were filled with thermal water and incubated at the same sites, with the contents of the tubes freely accessible to the hydrothermal fluid. As a result, several enrichment cultures growing in situ on different polymeric substrates were obtained. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene fragments obtained after PCR with Bacteria-specific primers showed that the bacterial communities developing on carbohydrates included the genera Caldicellulosiruptor and Dictyoglomus and that those developing on proteins contained members of the Thermotogales order. DGGE analysis performed after PCR with Archaea- and Crenarchaeota-specific primers showed that archaea related to uncultured environmental clones, particularly those of the Crenarchaeota phylum, were present in both carbohydrate- and protein-degrading communities. Five isolates obtained from in situ enrichments or corresponding natural samples of water and sediments represented the bacterial genera Dictyoglomus and Caldanaerobacter as well as new archaea of the Crenarchaeota phylum. Thus, in situ enrichment and consequent isolation showed the diversity of thermophilic prokaryotes competing for biopolymers in microbial communities of terrestrial Hot Springs.

  • Biodiversity of Thermophilic Prokaryotes with Hydrolytic Activities in Hot Springs of Uzon Caldera, Kamchatka (Russia)
    Applied and Environmental Microbiology, 2009
    Co-Authors: Ilya V Kublanov, Anna A Perevalova, G B Slobodkina, Aleksander V Lebedinsky, Salima Kh Bidzhieva, T V Kolganova, E N Kaliberda, L D Rumsh, Thomas Haertle, Elizaveta Bonch-osmolovskaya
    Abstract:

    Samples of water from the Hot Springs of Uzon Caldera with temperatures from 68 to 87 C and pHs of 4.1 to 7.0, supplemented with proteinaceous (albumin, casein, or alpha- or beta-keratin) or carbohydrate (cellulose, carboxymethyl cellulose, chitin, or agarose) biological polymers, were filled with thermal water and incubated at the same sites, with the contents of the tubes freely accessible to the hydrothermal fluid. As a result, several enrichment cultures growing in situ on different polymeric substrates were obtained. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene fragments obtained after PCR with Bacteria-specific primers showed that the bacterial communities developing on carbohydrates included the genera Caldicellulosiruptor and Dictyoglomus and that those developing on proteins contained members of the Thermotogales order. DGGE analysis performed after PCR with Archaea- and Crenarchaeota-specific primers showed that archaea related to uncultured environmental clones, particularly those of the Crenarchaeota phylum, were present in both carbohydrate- and protein-degrading communities. Five isolates obtained from in situ enrichments or corresponding natural samples of water and sediments represented the bacterial genera Dictyoglomus and Caldanaerobacter as well as new archaea of the Crenarchaeota phylum. Thus, in situ enrichment and consequent isolation showed the diversity of thermophilic prokaryotes competing for biopolymers in microbial communities of terrestrial Hot Springs.

  • Biodiversity of thermophilic prokaryotes with hydrolytic activities in Hot Springs of Uzon Caldera, Kamchatka (Russia).
    Applied and environmental microbiology, 2008
    Co-Authors: Ilya V Kublanov, Anna A Perevalova, G B Slobodkina, Aleksander V Lebedinsky, Salima Kh Bidzhieva, T V Kolganova, E N Kaliberda, L D Rumsh, Thomas Haertle, Elizaveta Bonch-osmolovskaya
    Abstract:

    Samples of water from the Hot Springs of Uzon Caldera with temperatures from 68 to 87 degrees C and pHs of 4.1 to 7.0, supplemented with proteinaceous (albumin, casein, or alpha- or beta-keratin) or carbohydrate (cellulose, carboxymethyl cellulose, chitin, or agarose) biological polymers, were filled with thermal water and incubated at the same sites, with the contents of the tubes freely accessible to the hydrothermal fluid. As a result, several enrichment cultures growing in situ on different polymeric substrates were obtained. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene fragments obtained after PCR with Bacteria-specific primers showed that the bacterial communities developing on carbohydrates included the genera Caldicellulosiruptor and Dictyoglomus and that those developing on proteins contained members of the Thermotogales order. DGGE analysis performed after PCR with Archaea- and Crenarchaeota-specific primers showed that archaea related to uncultured environmental clones, particularly those of the Crenarchaeota phylum, were present in both carbohydrate- and protein-degrading communities. Five isolates obtained from in situ enrichments or corresponding natural samples of water and sediments represented the bacterial genera Dictyoglomus and Caldanaerobacter as well as new archaea of the Crenarchaeota phylum. Thus, in situ enrichment and consequent isolation showed the diversity of thermophilic prokaryotes competing for biopolymers in microbial communities of terrestrial Hot Springs.

  • Distribution of Crenarchaeota representatives in terrestrial Hot Springs of Russia and Iceland.
    Applied and environmental microbiology, 2008
    Co-Authors: Anna A Perevalova, Christa Schleper, T V Kolganova, Elizaveta Bonch-osmolovskaya, Nils-kåre Birkeland, Aleksander V Lebedinsky
    Abstract:

    Culture-independent (PCR with Crenarchaeota-specific primers and subsequent denaturing gradient gel electrophoresis) and culture-dependent approaches were used to study the diversity of Crenarchaeota in terrestrial Hot Springs of the Kamchatka Peninsula and the Lake Baikal region (Russia) and of Iceland. Among the phylotypes detected there were relatives of both cultured (mainly hyperthermophilic) and uncultured Crenarchaeota. It was found that there is a large and diverse group of uncultured Crenarchaeota that inhabit terrestrial Hot Springs with moderate temperatures (55 to 70°C). Two of the lineages of this group were given phenotypic characterization, one as a result of cultivation in an enrichment culture and another one after isolation of a pure culture, “Fervidococcus fontis,” which proved to be a moderately thermophilic, neutrophilic (optimum pH of 6.0 to 7.5), anaerobic organotroph.

Aleksander V Lebedinsky - One of the best experts on this subject based on the ideXlab platform.

  • Anaerobic transformation of carbon monoxide by microbial communities of Kamchatka Hot Springs
    Extremophiles, 2011
    Co-Authors: Tatiana V. Kochetkova, Aleksander V Lebedinsky, T V Kolganova, Elizaveta Bonch-osmolovskaya, Igor I Rusanov, Nikolay V. Pimenov, Tatyana G. Sokolova
    Abstract:

    Carbon monoxide (CO) is one of the common gaseous compounds found in Hot volcanic environments. It is known to serve as the growth substrate for a number of thermophilic prokaryotes, both aerobic and anaerobic. The goal of this work was to study the process of anaerobic transformation of CO by microbial communities inhabiting natural thermal environments: Hot Springs of Uzon Caldera, Kamchatka. The anaerobic microbial community of Treshchinny Spring (80°C, pH 6.5) was found to exhibit two peaks of affinity for CO ( K _S1 = 54 nM and K _S2 = 1 μM). The actual rate of anaerobic CO transformation by the microbial community of this spring, calculated after obtaining the concentration dependence curve and extrapolated to the natural concentration of CO dissolved in the Hot spring water (20 nM), was found to be 120 μmol l^−1 of sediment day^−1. In all the Hot Springs studied, more than 90% of the carbon of ^14CO upon anaerobic incubation was recovered as ^14CO_2. From 1 to 5% of ^14CO was transformed to volatile fatty acids (VFA). The number of microorganisms capable of anaerobic CO oxidation determined by dilution-to-extinction method reached 10^6 cells ml^−1 of sediment. CO-transforming anaerobic thermophilic microorganisms isolated from the Springs under study exhibited hydrogenogenic type of CO oxidation and belonged to the bacterial genera Carboxydocella and Dictyoglomus. These data suggest a significant role of hydrogenogenic carboxydotrophic prokaryotes in anaerobic CO transformation in Uzon Caldera Hot Springs.

  • biodiversity of thermophilic prokaryotes with hydrolytic activities in Hot Springs of uzon caldera kamchatka russia
    Applied and Environmental Microbiology, 2009
    Co-Authors: Ilya V Kublanov, Anna A Perevalova, G B Slobodkina, Aleksander V Lebedinsky, Salima Kh Bidzhieva, T V Kolganova, E N Kaliberda, L D Rumsh, Thomas Haertle, Elizaveta A Bonchosmolovskaya
    Abstract:

    Samples of water from the Hot Springs of Uzon Caldera with temperatures from 68 to 87°C and pHs of 4.1 to 7.0, supplemented with proteinaceous (albumin, casein, or α- or β-keratin) or carbohydrate (cellulose, carboxymethyl cellulose, chitin, or agarose) biological polymers, were filled with thermal water and incubated at the same sites, with the contents of the tubes freely accessible to the hydrothermal fluid. As a result, several enrichment cultures growing in situ on different polymeric substrates were obtained. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene fragments obtained after PCR with Bacteria-specific primers showed that the bacterial communities developing on carbohydrates included the genera Caldicellulosiruptor and Dictyoglomus and that those developing on proteins contained members of the Thermotogales order. DGGE analysis performed after PCR with Archaea- and Crenarchaeota-specific primers showed that archaea related to uncultured environmental clones, particularly those of the Crenarchaeota phylum, were present in both carbohydrate- and protein-degrading communities. Five isolates obtained from in situ enrichments or corresponding natural samples of water and sediments represented the bacterial genera Dictyoglomus and Caldanaerobacter as well as new archaea of the Crenarchaeota phylum. Thus, in situ enrichment and consequent isolation showed the diversity of thermophilic prokaryotes competing for biopolymers in microbial communities of terrestrial Hot Springs.

  • Biodiversity of Thermophilic Prokaryotes with Hydrolytic Activities in Hot Springs of Uzon Caldera, Kamchatka (Russia)
    Applied and Environmental Microbiology, 2009
    Co-Authors: Ilya V Kublanov, Anna A Perevalova, G B Slobodkina, Aleksander V Lebedinsky, Salima Kh Bidzhieva, T V Kolganova, E N Kaliberda, L D Rumsh, Thomas Haertle, Elizaveta Bonch-osmolovskaya
    Abstract:

    Samples of water from the Hot Springs of Uzon Caldera with temperatures from 68 to 87 C and pHs of 4.1 to 7.0, supplemented with proteinaceous (albumin, casein, or alpha- or beta-keratin) or carbohydrate (cellulose, carboxymethyl cellulose, chitin, or agarose) biological polymers, were filled with thermal water and incubated at the same sites, with the contents of the tubes freely accessible to the hydrothermal fluid. As a result, several enrichment cultures growing in situ on different polymeric substrates were obtained. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene fragments obtained after PCR with Bacteria-specific primers showed that the bacterial communities developing on carbohydrates included the genera Caldicellulosiruptor and Dictyoglomus and that those developing on proteins contained members of the Thermotogales order. DGGE analysis performed after PCR with Archaea- and Crenarchaeota-specific primers showed that archaea related to uncultured environmental clones, particularly those of the Crenarchaeota phylum, were present in both carbohydrate- and protein-degrading communities. Five isolates obtained from in situ enrichments or corresponding natural samples of water and sediments represented the bacterial genera Dictyoglomus and Caldanaerobacter as well as new archaea of the Crenarchaeota phylum. Thus, in situ enrichment and consequent isolation showed the diversity of thermophilic prokaryotes competing for biopolymers in microbial communities of terrestrial Hot Springs.

  • Biodiversity of thermophilic prokaryotes with hydrolytic activities in Hot Springs of Uzon Caldera, Kamchatka (Russia).
    Applied and environmental microbiology, 2008
    Co-Authors: Ilya V Kublanov, Anna A Perevalova, G B Slobodkina, Aleksander V Lebedinsky, Salima Kh Bidzhieva, T V Kolganova, E N Kaliberda, L D Rumsh, Thomas Haertle, Elizaveta Bonch-osmolovskaya
    Abstract:

    Samples of water from the Hot Springs of Uzon Caldera with temperatures from 68 to 87 degrees C and pHs of 4.1 to 7.0, supplemented with proteinaceous (albumin, casein, or alpha- or beta-keratin) or carbohydrate (cellulose, carboxymethyl cellulose, chitin, or agarose) biological polymers, were filled with thermal water and incubated at the same sites, with the contents of the tubes freely accessible to the hydrothermal fluid. As a result, several enrichment cultures growing in situ on different polymeric substrates were obtained. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene fragments obtained after PCR with Bacteria-specific primers showed that the bacterial communities developing on carbohydrates included the genera Caldicellulosiruptor and Dictyoglomus and that those developing on proteins contained members of the Thermotogales order. DGGE analysis performed after PCR with Archaea- and Crenarchaeota-specific primers showed that archaea related to uncultured environmental clones, particularly those of the Crenarchaeota phylum, were present in both carbohydrate- and protein-degrading communities. Five isolates obtained from in situ enrichments or corresponding natural samples of water and sediments represented the bacterial genera Dictyoglomus and Caldanaerobacter as well as new archaea of the Crenarchaeota phylum. Thus, in situ enrichment and consequent isolation showed the diversity of thermophilic prokaryotes competing for biopolymers in microbial communities of terrestrial Hot Springs.

  • Distribution of Crenarchaeota representatives in terrestrial Hot Springs of Russia and Iceland.
    Applied and environmental microbiology, 2008
    Co-Authors: Anna A Perevalova, Christa Schleper, T V Kolganova, Elizaveta Bonch-osmolovskaya, Nils-kåre Birkeland, Aleksander V Lebedinsky
    Abstract:

    Culture-independent (PCR with Crenarchaeota-specific primers and subsequent denaturing gradient gel electrophoresis) and culture-dependent approaches were used to study the diversity of Crenarchaeota in terrestrial Hot Springs of the Kamchatka Peninsula and the Lake Baikal region (Russia) and of Iceland. Among the phylotypes detected there were relatives of both cultured (mainly hyperthermophilic) and uncultured Crenarchaeota. It was found that there is a large and diverse group of uncultured Crenarchaeota that inhabit terrestrial Hot Springs with moderate temperatures (55 to 70°C). Two of the lineages of this group were given phenotypic characterization, one as a result of cultivation in an enrichment culture and another one after isolation of a pure culture, “Fervidococcus fontis,” which proved to be a moderately thermophilic, neutrophilic (optimum pH of 6.0 to 7.5), anaerobic organotroph.