The Experts below are selected from a list of 249 Experts worldwide ranked by ideXlab platform
Nadine Alvarez - One of the best experts on this subject based on the ideXlab platform.
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Passive administration of purified secretory IgA from Human Colostrum induces protection against Mycobacterium tuberculosis in a murine model of progressive pulmonary infection
BMC Immunology, 2013Co-Authors: Nadine Alvarez, Oscar Otero, Frank Camacho, Reinier Borrero, Yanely Tirado, Alina Puig, Alicia Aguilar, Cesar Rivas, Axel Cervantes, Gustavo Falero-díazAbstract:Background Immunoglobulin A is the most abundant isotype in secretions from mucosal surfaces of the gastrointestinal, respiratory and genitourinary tracts and in external secretions such as Colostrum, breast milk, tears and saliva. The high concentration of Human secretory IgA (hsIgA) in Human Colostrum strongly suggests that it should play an important role in the passive immune protection against gastrointestinal and respiratory infections. Materials and methods Human secretory IgA was purified from Colostrum. The reactivity of hsIgA against mycobacterial antigens and its protective capacity against mycobacterial infection was evaluated. Results The passive administration of hsIgA reduces the pneumonic area before challenge with M. tuberculosis. The intratracheal administration of M. tuberculosis preincubated with hsIgA to mice greatly reduced the bacterial load in the lungs and diminished lung tissue injury. Conclusions HsIgA purified from Colostrum protects against M. tuberculosis infection in an experimental mouse model.
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passive administration of purified secretory iga from Human Colostrum induces protection against mycobacterium tuberculosis in a murine model of progressive pulmonary infection
BMC Immunology, 2013Co-Authors: Nadine Alvarez, Oscar Otero, Frank Camacho, Reinier Borrero, Yanely Tirado, Alina Puig, Alicia Aguilar, Cesar Rivas, Axel Cervantes, Gustavo FalerodiazAbstract:Immunoglobulin A is the most abundant isotype in secretions from mucosal surfaces of the gastrointestinal, respiratory and genitourinary tracts and in external secretions such as Colostrum, breast milk, tears and saliva. The high concentration of Human secretory IgA (hsIgA) in Human Colostrum strongly suggests that it should play an important role in the passive immune protection against gastrointestinal and respiratory infections. Human secretory IgA was purified from Colostrum. The reactivity of hsIgA against mycobacterial antigens and its protective capacity against mycobacterial infection was evaluated. The passive administration of hsIgA reduces the pneumonic area before challenge with M. tuberculosis. The intratracheal administration of M. tuberculosis preincubated with hsIgA to mice greatly reduced the bacterial load in the lungs and diminished lung tissue injury. HsIgA purified from Colostrum protects against M. tuberculosis infection in an experimental mouse model.
Yasuyuki Sanai - One of the best experts on this subject based on the ideXlab platform.
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Variation of major neutral oligosaccharides levels in Human Colostrum
European Journal of Clinical Nutrition, 2008Co-Authors: S Asakuma, Masato Akahori, Hiroki Obayashi, I Arai, Tadasu Urashima, K Kimura, T. Nakamura, Y. Watanabe, Yasuyuki SanaiAbstract:Objectives:Human Colostrum is known to be important for the protection of infants against infection by pathogenic microorganisms. This protection is thought to be due, partially, to various neutral and acidic oligosaccharides that are present in Colostrum and milk. However, the concentrations of each of the oligosaccharide of Human Colostrum have not yet been determined. The aim of this present study was to determine the concentration of each of the major neutral oligosaccharide for three consecutive days from the start of lactation.Method:We analyzed the level of each neutral oligosaccharide in Human Colostrum, for three consecutive days from the start of lactation, obtained from 12 healthy Japanese women (ranging in age from 21 to 35 years; primipara 6 and multipara 6). The ABO blood groups of the donors were determined: A, three; B, three; O, five; AB, one. The determined Human milk oligosaccharides were 2′-fucosyllactose (2′-FL), 3-fucosyllactose (3-FL), lactodifucotetraose (LDFT), lacto- N -tetraose (LNT), lacto- N -neotetraose (LNnT), three lacto- N -fucopentaose (LNFP I, II and III) and two lacto- N -difucohexaose (LNFDH I and II) using high-performance liquid chromatography (HPLC) with two derivatization techniques.Results:The concentrations of 2′-FL and LDFT in Colostrum on day 1 were significantly higher than those on days 2 and 3 ( P
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variation of major neutral oligosaccharides levels in Human Colostrum
European Journal of Clinical Nutrition, 2008Co-Authors: S Asakuma, Masato Akahori, Hiroki Obayashi, I Arai, Tadasu Urashima, K Kimura, T. Nakamura, Y. Watanabe, Yasuyuki SanaiAbstract:Human Colostrum is known to be important for the protection of infants against infection by pathogenic microorganisms. This protection is thought to be due, partially, to various neutral and acidic oligosaccharides that are present in Colostrum and milk. However, the concentrations of each of the oligosaccharide of Human Colostrum have not yet been determined. The aim of this present study was to determine the concentration of each of the major neutral oligosaccharide for three consecutive days from the start of lactation. We analyzed the level of each neutral oligosaccharide in Human Colostrum, for three consecutive days from the start of lactation, obtained from 12 healthy Japanese women (ranging in age from 21 to 35 years; primipara 6 and multipara 6). The ABO blood groups of the donors were determined: A, three; B, three; O, five; AB, one. The determined Human milk oligosaccharides were 2′-fucosyllactose (2′-FL), 3-fucosyllactose (3-FL), lactodifucotetraose (LDFT), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT), three lacto-N-fucopentaose (LNFP I, II and III) and two lacto-N-difucohexaose (LNFDH I and II) using high-performance liquid chromatography (HPLC) with two derivatization techniques. The concentrations of 2′-FL and LDFT in Colostrum on day 1 were significantly higher than those on days 2 and 3 (P<0.05). An increase in LNT was observed on day 3 compared with day 1 (P<0.05). These changes in concentrations of 2’-FL, LDFT and LNT may reflect the requirements for prebiotics and anti-infection agents by Human infants during early lactation.
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Sialyl Oligosaccharides of Human Colostrum: Changes in Concentration during the First Three Days of Lactation
Bioscience biotechnology and biochemistry, 2007Co-Authors: S Asakuma, Yasuyuki Sanai, I Arai, T. Nakamura, Maiko Akahori, Kazumasa Kimura, Yoko Watanabe, Makoto Tsunemi, Tadasu UrashimaAbstract:Sialyl oligosaccharides of Human milk/Colostrum are generally believed to be of biological significance, for example with respect to anti-adhesion of pathogenic organism, providing precursors for biosynthesis of brain, and so on. However, the levels of each of the sialyl oligosaccharides in Human Colostrum have not so far been determined. The present study was designed to determine the concentrations of nine major sialyl oligosaccharides in Human Colostrum, collected during the first 3 d (days 1–3) from the start of lactation. We found that the concentration of 3′-sialyllactose was significantly higher on day 1 than on day 2 and 3, but the levels of 6′-sialyllactose and sialyllacto-N-tetraose a were higher on day 3 than on day 1. These results are consistent with the view that during the first 3 d of lactation, the concentration of sialyl oligosaccharides in Human Colostrum change in accordance with the physiological demands of newborn infants.
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Variation of major neutral oligosaccharides levels in Human Colostrum
European journal of clinical nutrition, 2007Co-Authors: S Asakuma, Masato Akahori, Hiroki Obayashi, I Arai, Tadasu Urashima, K Kimura, T. Nakamura, Y. Watanabe, Yasuyuki SanaiAbstract:Human Colostrum is known to be important for the protection of infants against infection by pathogenic microorganisms. This protection is thought to be due, partially, to various neutral and acidic oligosaccharides that are present in Colostrum and milk. However, the concentrations of each of the oligosaccharide of Human Colostrum have not yet been determined. The aim of this present study was to determine the concentration of each of the major neutral oligosaccharide for three consecutive days from the start of lactation. We analyzed the level of each neutral oligosaccharide in Human Colostrum, for three consecutive days from the start of lactation, obtained from 12 healthy Japanese women (ranging in age from 21 to 35 years; primipara 6 and multipara 6). The ABO blood groups of the donors were determined: A, three; B, three; O, five; AB, one. The determined Human milk oligosaccharides were 2′-fucosyllactose (2′-FL), 3-fucosyllactose (3-FL), lactodifucotetraose (LDFT), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT), three lacto-N-fucopentaose (LNFP I, II and III) and two lacto-N-difucohexaose (LNFDH I and II) using high-performance liquid chromatography (HPLC) with two derivatization techniques. The concentrations of 2′-FL and LDFT in Colostrum on day 1 were significantly higher than those on days 2 and 3 (P
Gustavo Falerodiaz - One of the best experts on this subject based on the ideXlab platform.
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passive administration of purified secretory iga from Human Colostrum induces protection against mycobacterium tuberculosis in a murine model of progressive pulmonary infection
BMC Immunology, 2013Co-Authors: Nadine Alvarez, Oscar Otero, Frank Camacho, Reinier Borrero, Yanely Tirado, Alina Puig, Alicia Aguilar, Cesar Rivas, Axel Cervantes, Gustavo FalerodiazAbstract:Immunoglobulin A is the most abundant isotype in secretions from mucosal surfaces of the gastrointestinal, respiratory and genitourinary tracts and in external secretions such as Colostrum, breast milk, tears and saliva. The high concentration of Human secretory IgA (hsIgA) in Human Colostrum strongly suggests that it should play an important role in the passive immune protection against gastrointestinal and respiratory infections. Human secretory IgA was purified from Colostrum. The reactivity of hsIgA against mycobacterial antigens and its protective capacity against mycobacterial infection was evaluated. The passive administration of hsIgA reduces the pneumonic area before challenge with M. tuberculosis. The intratracheal administration of M. tuberculosis preincubated with hsIgA to mice greatly reduced the bacterial load in the lungs and diminished lung tissue injury. HsIgA purified from Colostrum protects against M. tuberculosis infection in an experimental mouse model.
Gustavo Falero-díaz - One of the best experts on this subject based on the ideXlab platform.
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Passive administration of purified secretory IgA from Human Colostrum induces protection against Mycobacterium tuberculosis in a murine model of progressive pulmonary infection
BMC Immunology, 2013Co-Authors: Nadine Alvarez, Oscar Otero, Frank Camacho, Reinier Borrero, Yanely Tirado, Alina Puig, Alicia Aguilar, Cesar Rivas, Axel Cervantes, Gustavo Falero-díazAbstract:Background Immunoglobulin A is the most abundant isotype in secretions from mucosal surfaces of the gastrointestinal, respiratory and genitourinary tracts and in external secretions such as Colostrum, breast milk, tears and saliva. The high concentration of Human secretory IgA (hsIgA) in Human Colostrum strongly suggests that it should play an important role in the passive immune protection against gastrointestinal and respiratory infections. Materials and methods Human secretory IgA was purified from Colostrum. The reactivity of hsIgA against mycobacterial antigens and its protective capacity against mycobacterial infection was evaluated. Results The passive administration of hsIgA reduces the pneumonic area before challenge with M. tuberculosis. The intratracheal administration of M. tuberculosis preincubated with hsIgA to mice greatly reduced the bacterial load in the lungs and diminished lung tissue injury. Conclusions HsIgA purified from Colostrum protects against M. tuberculosis infection in an experimental mouse model.
P Mcwilliams-koeppen - One of the best experts on this subject based on the ideXlab platform.
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Soluble tumor necrosis factor-alpha (TNF-alpha) receptors in Human Colostrum and milk bind to TNF-alpha and neutralize TNF-alpha bioactivity.
Pediatric research, 1998Co-Authors: E S Buescher, P Mcwilliams-koeppenAbstract:We used column chromatography, affinity binding, and bioassay methods to address whether the soluble tumor necrosis factor (TNF)-alpha receptors present in Human Colostrum and milk bind to and modify TNF-alpha bioactivity. In gel chromatography experiments, soluble TNF-alpha receptor I (sTNFRI) and sTNFRII in Human Colostrum sequentially increased their molecular sizes from 49 kD to 71 kD and 60 kD, respectively, after addition of increasing molar excesses of recombinant TNF-alpha. Application of Colostrum to a TNF-alpha affinity matrix followed by washing and elution resulted in 2925-fold enrichment of sTNFRI, consistent with sTNFRI binding to the TNF-alpha affinity matrix. In other samples of Colostrum and milk, the content of both sTNFRI and sTNFRII decreased significantly after passage over the matrix, but the material eluted from the matrix lost the ability to rebind to the TNF-alpha and was not active in a WEHI-13var bioassay for TNF-alpha. Specimens of Human Colostrum and milk diluted 1:16 shifted the LD50 for TNF-alpha 4-fold in this bioassay, and milk protection of WEHI-13var cells against TNF-alpha was significantly diminished after passage down the TNF-alpha affinity matrix (p < 0.001). Affinity purification of milk sTNFRI using polyclonal anti-sTNFRI produced fractions containing proteins of 30 kD, which could be visualized by Western blot using polyclonal anti-sTNFRI. Addition of this fraction to the WEHI-13var bioassay reversed the effects of 10 pg/mL TNF-alpha in the assay. These data demonstrate that sTNFRI and II from Human Colostrum and milk bind to TNF-alpha, that both Colostrum and milk interfere with the bioactivity of TNF-alpha, and that affinity-purified sTNFRI from Human milk blocks the bioactivity of TNF-alpha. These effects may contribute to the anti-inflammatory character of Human Colostrum and milk.