The Experts below are selected from a list of 39078 Experts worldwide ranked by ideXlab platform
Preben Christensen - One of the best experts on this subject based on the ideXlab platform.
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the sperm chromatin structure assay as a diagnostic tool in the Human Fertility clinic
Human Reproduction, 2006Co-Authors: G B Boehansen, Jens Fedder, Annette Kjaer Ersboll, Preben ChristensenAbstract:BACKGROUND: Sperm DNA integrity has been shown to be necessary for achieving and sustaining embryo development. The objective was to evaluate the sperm chromatin structure assay (SCSA) as a diagnostic tool in clinical practice for intrauterine insemination (IUI), in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) treatments. METHODS: A total of 385 semen samples from 234 couples were frozen for SCSA, and smears were prepared for morphology: 48 IUI, 139 IVF and 47 ICSI. The main SCSA variables were DNA fragmentation index (DFI), standard deviation of DFI (SD-DFI) and high DNA stainability (HDS), and the reproductive outcomes were biochemical pregnancy (BP), clinical pregnancy (CP) and implantation ratio (IR). RESULTS: The results showed no significant difference in the Fertility variables BP, CP and IR when 27%, and six couples in ICSI treatment achieved CP full-term. DFI >27% had a high prognostic power for predicting no CP for IVF patients, with a specificity of 97%. Couples diagnosed with male inFertility had a significantly higher level of DFI compared to couples with idiopathic Fertility. Sperm head morphology showed low but significant correlations with the SCSA variables. CONCLUSION: SCSA is a useful tool in andrological diagnosis and contributes with a prognosis for the Fertility outcome of conventional IVF. Although full-term pregnancy can be achieved with assisted reproductive techniques with a DFI >27%, the probability of a successful pregnancy may be reduced.
O P Claussen - One of the best experts on this subject based on the ideXlab platform.
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utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the Human Fertility clinic
Human Reproduction, 1999Co-Authors: D Marshall, M J Zinaman, E D Clegg, K Purvis, P De Angelis, O P ClaussenAbstract:The sperm chromatin structure assay (SCSA) was used to measure over 500 Human semen samples from two independent studies: Study I, 402 samples from 165 presumably fertile couples wishing to achieve pregnancy over 12 menstrual cycles; Study II, samples from 115 patients seeking Fertility counselling. The SCSA measures susceptibility to DNA denaturation in situ in spermatozoa exposed to acid for 30 s, followed by acridine orange staining. SCSA data from the male partners of 73 couples (group 1) achieving pregnancy during months 1‐3 of Study I were used as the standard of ‘sperm chromatin compatible with high Fertility’ and were significantly different from those of 40 couples (group 3) achieving pregnancy in months 4‐12 (P < 0.01) and those of male partners of 31 couples (group 4) not achieving pregnancy (P < 0.001). Group 2 contained couples who had a miscarriage. SCSA values for Study II were almost twice that of the Study I Fertility standards. Within-couple repeatability tended to be less for group 3 than for groups 1, 2 or 4. Based on logistic regression, spermatozoa with denatured DNA (cells outside the main population, COMPa t) were the best predictor for whether a couple would not achieve pregnancy. Some 84% of males in group 1 had COMPat <15%, while no couples achieved pregnancy in group 1 with ‡30% COMPat, a threshold level considered not compatible with good Fertility. Using selected cut-off values for chromatin integrity, the SCSA data predicted seven of 18 miscarriages (39%).
Carlos Simon - One of the best experts on this subject based on the ideXlab platform.
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single cell transcriptomic atlas of the Human endometrium during the menstrual cycle
Nature Medicine, 2020Co-Authors: Wanxin Wang, Felipe Vilella, Pilar Alama, I Moreno, Marco Mignardi, Alina Isakova, Wenying Pan, Carlos Simon, Stephen R QuakeAbstract:In a Human menstrual cycle the endometrium undergoes remodeling, shedding and regeneration, all of which are driven by substantial gene expression changes in the underlying cellular hierarchy. Despite its importance in Human Fertility and regenerative biology, our understanding of this unique type of tissue homeostasis remains rudimentary. We characterized the transcriptomic transformation of Human endometrium at single-cell resolution across the menstrual cycle, resolving cellular heterogeneity in multiple dimensions. We profiled the behavior of seven endometrial cell types, including a previously uncharacterized ciliated cell type, during four major phases of endometrial transformation, and found characteristic signatures for each cell type and phase. We discovered that the Human window of implantation opens with an abrupt and discontinuous transcriptomic activation in the epithelia, accompanied with a widespread decidualization feature in the stromal fibroblasts. Our study provides a high-resolution molecular and cellular characterization of Human endometrial transformation across the menstrual cycle, providing insights into this essential physiological process.
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single cell rnaseq provides a molecular and cellular cartography of changes to the Human endometrium through the menstrual cycle
Fertility and Sterility, 2018Co-Authors: Wanxin Wang, Felipe Vilella, I Moreno, Wenying Pan, Carlos Simon, Stephen R QuakeAbstract:In a Human menstrual cycle, the endometrium undergoes remodeling, shedding, and regeneration which are driven by substantial gene expression changes in the underlying cellular hierarchy. Despite its importance in Human Fertility and regenerative biology, mechanistic understanding of this unique type of tissue homeostasis remains rudimentary. Here, we characterized the transcriptomic transformation of Human endometrium at single cell resolution, dissecting multidimensional cellular heterogeneity of the tissue across the entire natural menstrual cycle. We analyzed 6 endometrial cell types, including a previously uncharacterized ciliated epithelial cell type, during four major phases of endometrial transformation, and found characteristic signatures for each cell type and phase. We discovered that Human window of implantation opens up with an abrupt and discontinuous transcriptomic activation in the epithelium, accompanied with widespread decidualized feature in the stroma. These data reveal signatures in the luminal and glandular epithelium during epithelial gland reconstruction, and suggest a mechanism for adult gland formation.
Olof Akre - One of the best experts on this subject based on the ideXlab platform.
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time trends in Human fecundability in sweden
Epidemiology, 2008Co-Authors: Thomas H Scheike, Lars Rylander, Ulf Stromberg, Michael Joffe, Lisbeth Carstensen, Niels Keiding, Olof AkreAbstract:BACKGROUND:: Trends in biologic Fertility are elusive. Possible negative trends in male reproductive health are still debated, and their effect on Human Fertility might be negligible. Time-to-pregnancy (TTP) is a functional measure of couple fecundability. METHODS:: We analyzed data on TTP among 832,000 primiparous women 20 years of age and older in the nationwide Swedish Medical Birth Registry from 1983 through 2002. This age restriction led to an exclusion of 10% of primiparous pregnancies. SubFertility (TTP >/=1 year) was analyzed as a function of maternal age, calendar time at initiation of attempt, and birth cohort-taking into account the truncation problems that are inherent in birth-based retrospective sampling. RESULTS:: SubFertility generally decreased over successive birth cohorts. When studied as a period effect, a transient increase in subFertility was seen in the early 1990s. SubFertility increased with age, except that for women in their late 1930s, an apparent decrease was observed, particularly among the early cohorts. CONCLUSION:: We found decreasing subFertility over time. We speculate that these patterns might be related to a Sweden-specific decrease over time in sexually transmitted diseases, to changes in sexual behavior induced by socioeconomic conditions, or to broader biologic or educational trends.
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Human Fertility does not decline evidence from sweden
Fertility and Sterility, 1999Co-Authors: Olof Akre, Sven Cnattingius, Reinhold Bergstrom, Ulrik Kvist, D Trichopoulos, Anders EkbomAbstract:Abstract Objective: To assess changes in Human Fertility over time. Design: Time-trend analyses and age-period-cohort modeling. Setting: Sweden, 1983–1993. Patient(s): All primiparous women aged ≥20 years during the study period. There were 401,653 women who were identified through the nationwide Medical Birth Register. Intervention(s): None. Main Outcome Measure(s): Risk of subFertility, defined as ≥1 year of involuntary childlessness. Result(s): SubFertility problems decreased dramatically over successive maternal birth cohorts. Further, the risk of subFertility increased with age and decreased with increasing formal education. Conclusion(s): A decrease in male Fertility cannot be ruled out on the basis of these results, but if present, it is minor and totally outweighed by other favorable developments. As the main explanation for our findings, we propose a decrease in the prevalence of secondary subFertility as a result of the eradication of gonorrhea.
Anders Ekbom - One of the best experts on this subject based on the ideXlab platform.
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Human Fertility does not decline evidence from sweden
Fertility and Sterility, 1999Co-Authors: Olof Akre, Sven Cnattingius, Reinhold Bergstrom, Ulrik Kvist, D Trichopoulos, Anders EkbomAbstract:Abstract Objective: To assess changes in Human Fertility over time. Design: Time-trend analyses and age-period-cohort modeling. Setting: Sweden, 1983–1993. Patient(s): All primiparous women aged ≥20 years during the study period. There were 401,653 women who were identified through the nationwide Medical Birth Register. Intervention(s): None. Main Outcome Measure(s): Risk of subFertility, defined as ≥1 year of involuntary childlessness. Result(s): SubFertility problems decreased dramatically over successive maternal birth cohorts. Further, the risk of subFertility increased with age and decreased with increasing formal education. Conclusion(s): A decrease in male Fertility cannot be ruled out on the basis of these results, but if present, it is minor and totally outweighed by other favorable developments. As the main explanation for our findings, we propose a decrease in the prevalence of secondary subFertility as a result of the eradication of gonorrhea.