Humoral Immune Response

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L R Haaheim - One of the best experts on this subject based on the ideXlab platform.

  • the Humoral Immune Response and protective efficacy of vaccination with inactivated split and whole influenza virus vaccines in balb c mice
    2006
    Co-Authors: Rebecca J. Cox, Arntove Hovden, Karl A Brokstad, Ewa A Szyszko, Abdullah S Madhun, L R Haaheim
    Abstract:

    Recently the urgency of developing a pandemic influenza vaccine has lead to the re-evaluation of the use of whole virus vaccine. We have compared the Humoral Immune Response and the protective efficacy of whole and split influenza virus vaccines in mice. Whole virus vaccine was more immunogenic particularly after the first dose of vaccine, generally eliciting higher numbers of systemic antibody secreting cells and an earlier and higher neutralising antibody Response. Immunisation with one dose of whole virus vaccine more effectively reduced viral shedding upon non-lethal homologous viral challenge, but two doses of split virus vaccine was most effective at limiting viral replication and this was correlated with high influenza specific serum IgG concentrations. The two vaccine formulations induced different T helper profiles particularly after one dose of vaccine; split virus vaccine induced a type 2 bias Response, whereas whole virus vaccine elicited a dominant type 1 Response.

  • an early Humoral Immune Response in peripheral blood following parenteral inactivated influenza vaccination
    1994
    Co-Authors: Rebecca J. Cox, L R Haaheim, Karl A Brokstad, Mark Zuckerman, John Wood, John S Oxford
    Abstract:

    The enzyme-linked immunospot assay was used to examine the Humoral Immune Response in 15 healthy volunteers immunized with either split or subunit inactivated trivalent influenza vaccine containing A/Beijing/353/89 (H3N2), A/Taiwan/1/86 (H1N1) and B/Yamagata/16/88. The rapidity of the individual B-cell and serum antibody Response was examined in lymphocyte and serum samples collected at various time intervals after vaccination. A rapid serological Response was detected with increases in antibody titre detected in the majority of volunteers by 7-8 days postvaccination. Influenza-specific plasma cells were detected as early as 4 days postvaccination, higher numbers of IgA and IgG antibody-secreting cells (ASC) were observed which peaked at 7-8 days postvaccination. The number of ASCs then declined, with low numbers of cells detected at 11 days postvaccination. Influenza-specific IgA ASCs were predominantly of the IgA1 subclass. This rapid Immune Response may have a bearing on future vaccination policies of unimmunized 'at risk groups' in times of high influenza activity.

Norbert Wagner - One of the best experts on this subject based on the ideXlab platform.

  • neonatally induced inactivation of the vascular cell adhesion molecule 1 gene impairs b cell localization and t cell dependent Humoral Immune Response
    2001
    Co-Authors: Christoph Leuker, Mark Labow, Werner Muller, Norbert Wagner
    Abstract:

    Vascular cellular adhesion molecule (VCAM)-1 is a membrane-bound cellular adhesion molecule that mediates adhesive interactions between hematopoietic progenitor cells and stromal cells in the bone marrow (BM) and between leukocytes and endothelial as well as dendritic cells. Since VCAM-1–deficient mice die embryonically, conditional VCAM-1 mutant mice were generated to analyze the in vivo function of this adhesion molecule. Here we show that interferon-induced Cre-loxP–mediated deletion of the VCAM-1 gene after birth efficiently ablates expression of VCAM-1 in most tissues like, for example, BM, lymphoid organs, and lung, but not in brain. Induced VCAM-1 deficiency leads to a reduction of immature B cells in the BM and to an increase of these cells in peripheral blood but not in lymphoid organs. Mature recirculating B cells are reduced in the BM. In a migration assay, the number of mature B cells that appears in the BM after intravenous injection is decreased. In addition, the Humoral Immune Response to a T cell–dependent antigen is impaired. VCAM-1 serves an important role for B cell localization and the T cell–dependent Humoral Immune Response.

Rebecca J. Cox - One of the best experts on this subject based on the ideXlab platform.

  • the Humoral Immune Response and protective efficacy of vaccination with inactivated split and whole influenza virus vaccines in balb c mice
    2006
    Co-Authors: Rebecca J. Cox, Arntove Hovden, Karl A Brokstad, Ewa A Szyszko, Abdullah S Madhun, L R Haaheim
    Abstract:

    Recently the urgency of developing a pandemic influenza vaccine has lead to the re-evaluation of the use of whole virus vaccine. We have compared the Humoral Immune Response and the protective efficacy of whole and split influenza virus vaccines in mice. Whole virus vaccine was more immunogenic particularly after the first dose of vaccine, generally eliciting higher numbers of systemic antibody secreting cells and an earlier and higher neutralising antibody Response. Immunisation with one dose of whole virus vaccine more effectively reduced viral shedding upon non-lethal homologous viral challenge, but two doses of split virus vaccine was most effective at limiting viral replication and this was correlated with high influenza specific serum IgG concentrations. The two vaccine formulations induced different T helper profiles particularly after one dose of vaccine; split virus vaccine induced a type 2 bias Response, whereas whole virus vaccine elicited a dominant type 1 Response.

  • an early Humoral Immune Response in peripheral blood following parenteral inactivated influenza vaccination
    1994
    Co-Authors: Rebecca J. Cox, L R Haaheim, Karl A Brokstad, Mark Zuckerman, John Wood, John S Oxford
    Abstract:

    The enzyme-linked immunospot assay was used to examine the Humoral Immune Response in 15 healthy volunteers immunized with either split or subunit inactivated trivalent influenza vaccine containing A/Beijing/353/89 (H3N2), A/Taiwan/1/86 (H1N1) and B/Yamagata/16/88. The rapidity of the individual B-cell and serum antibody Response was examined in lymphocyte and serum samples collected at various time intervals after vaccination. A rapid serological Response was detected with increases in antibody titre detected in the majority of volunteers by 7-8 days postvaccination. Influenza-specific plasma cells were detected as early as 4 days postvaccination, higher numbers of IgA and IgG antibody-secreting cells (ASC) were observed which peaked at 7-8 days postvaccination. The number of ASCs then declined, with low numbers of cells detected at 11 days postvaccination. Influenza-specific IgA ASCs were predominantly of the IgA1 subclass. This rapid Immune Response may have a bearing on future vaccination policies of unimmunized 'at risk groups' in times of high influenza activity.

Ragnar Freyr Ingvarsson - One of the best experts on this subject based on the ideXlab platform.

  • Humoral Immune Response to sars cov 2 in iceland
    2020
    Co-Authors: Daniel F Gudbjartsson, Gudmundur L Norddahl, Pall Melsted, Kristbjorg Gunnarsdottir, Hilma Holm, Elias Eythorsson, Asgeir O Arnthorsson, Dadi Helgason, Kristbjorg Bjarnadottir, Ragnar Freyr Ingvarsson
    Abstract:

    Abstract Background Little is known about the nature and durability of the Humoral Immune Response to infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Methods We measure...

Claudine Schiff - One of the best experts on this subject based on the ideXlab platform.

  • galectin 1 modulates plasma cell homeostasis and regulates the Humoral Immune Response
    2013
    Co-Authors: Adrienne Anginot, Marion Espeli, Lionel Chasson, Stephane J C Mancini, Claudine Schiff
    Abstract:

    Galectin-1 (GAL1) is an S-type lectin with multiple functions, including the control of B cell homeostasis. GAL1 expression was reported to be under the control of the plasma cell master regulator BLIMP-1. GAL1 was detected at the protein level in LPS-stimulated B cells and was shown to promote Ig secretion in vitro. However, the pattern of GAL1 expression and function of GAL1 in B cells in vivo are still unclear. In this study, we show that, among B cells, GAL1 is only expressed by differentiating plasma cells following T-dependent or T-independent immunization. Using GAL1-deficient mice we demonstrate that GAL1 expression is required for the maintenance of Ag-specific Ig titers and Ab-secreting cell numbers. Using an in vitro differentiation assay we find that GAL1-deficient plasmablasts can develop normally but die rapidly, through caspase 8 activation, under serum starvation–induced death conditions. TUNEL assays show that in vivo–generated GAL1-deficient plasma cells exhibit an increased sensitivity to apoptosis. Taken together, our data indicate that endogenous GAL1 supports plasma cell survival and participates in the regulation of the Humoral Immune Response.