The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Manuela Zavatti - One of the best experts on this subject based on the ideXlab platform.
-
Increased sexual motivation in female rats treated with Humulus lupulus L. extract.
Journal of Ethnopharmacology, 2011Co-Authors: Vittoria Di Viesti, Manuela Zavatti, Augusta Benelli, Gianluca Carnevale, Paola ZanoliAbstract:Abstract Aim of the study To evaluate the influence of Humulus lupulus extract on sexual behavior in female rats. Materials and methods Ovariectomized rats hormonally primed with estradiol benzoate (1.5 μg/rat) and progesterone (500 μg/rat) were acutely treated by oral gavage with Humulus lupulus extract dosed at 5, 10 and 25 mg/kg and then tested for partner preference and sexual receptivity. Results The administration of Humulus lupulus extract at the highest dose significantly increased the preference for the stimulus male during the partner preference test and the number of proceptive behaviors during the receptivity test, without affecting the lordosis response. Conclusions Humulus lupulus extract increased sexual motivation in hormone-primed female rats.
-
Experimental evidence of the anaphrodisiac activity of Humulus lupulus L. in naïve male rats.
Journal of Ethnopharmacology, 2009Co-Authors: Paola Zanoli, Manuela Zavatti, Augusta Benelli, Marianna Rivasi, Rossella Avallone, Mario BaraldiAbstract:Abstract Ethnopharmacological relevance In the folk medicine Humulus lupulus L. (hops) is mainly recommended as a mild sedative with antispasmodic and digestive properties. It is also reputed to exert an anaphrodisiac effect but it is still lacking the experimental evidence of this activity. Aim of the study To evaluate the influence of Humulus lupulus extract on sexual behavior of both naive and sexually potent male rats; thereafter to investigate the role of 8-prenylnarigenin (8-PN) in the effect displayed by the hop extract. Materials and methods Sprague–Dawley male rats both naive and sexually potent were acutely administered with the hop extract dosed at 5, 10, 25 and 50 mg/kg. In addition the extract was administered daily for 10 consecutive days at the dose of 0.25 mg/kg/day in sexually potent animals. The pure compound 8-PN was acutely administered in naive rats at the dosages of 5, 12.5 and 25 μg/kg. All the animals were screened for their sexual behavior manifestation during the mating test. Results In naive rats the acute administration of Humulus lupulus extract at the doses of 25 and 50 mg/kg significantly reduced the percentage of mounting and ejaculating animals, in comparison to vehicle controls. The other parameters recorded during the mating test were not affected by the hop extract. In sexually potent rats nor the acute neither the repeated administration of the extract modified their copulatory behavior. The pure compound 8-PN failed to influence male sexual behavior of naive rats. Conclusion Humulus lupulus extract exerted an anaphrodisiac effect only in naive rats by inhibiting their mounting and ejaculating behavior. The presence of 8-PN in the extract could be only partially involved in the observed anaphrodisiac effect.
-
pharmacognostic and pharmacological profile of Humulus lupulus l
Journal of Ethnopharmacology, 2008Co-Authors: Paola Zanoli, Manuela ZavattiAbstract:The present review describes the morphological, phytochemical and ethnopharmacological aspects of Humulus lupulus L. (Cannabinaceae) and summarizes the most interesting findings obtained in the preclinical and clinical research related to the plant. The female inflorescences of Humulus lupulus (hops), well-known as bittering agent in brewing industry, have long been used in traditional medicine mainly to treat sleep disturbances. However the sedative activity is still under investigation in order to recognize the active principles responsible for the neuropharmacological effects observed in laboratory animals, and their mechanism of action. Here we report the data from our experiments as well as those obtained from other researchers, focusing on the variability of the results. Other traditional applications of hops as stomachic, antibacterial and antifungal remedy have been supported by in vivo and/or in vitro investigations. In recent years some prenylated chalcones present in hops have received much attention for their biological effects: in particular, xanthohumol has been shown to exert cancer chemopreventive activity in in vitro experiments, while 8-prenylnaringenin has been characterized as one of the most potent phytoestrogens isolated until now. Nevertheless much additional work is needed to open up new biomedical application of these compounds.
-
new insight in the neuropharmacological activity of Humulus lupulus l
Journal of Ethnopharmacology, 2005Co-Authors: Paola Zanoli, Manuela Zavatti, Marianna Rivasi, F Brusiani, Mario BaraldiAbstract:Abstract The purpose of the present study was to investigate the effects of Humulus lupulus CO2 extract and its fraction containing α-acids on the central nervous system of rats. Both tested substances were able to prolong pentobarbital sleeping time, without affecting the latency to the loss of the righting reflex. This effect was dose-dependent, starting from a minimal dose of 10 mg/kg. Neither the extract nor its α-acid fraction affected the locomotor activity in the open field test or exerted an anxiolytic effect in rats submitted to the elevated plus-maze test. Interestingly both compounds reduced the immobility time during the behavioral despair test when administered three times (24, 5 and 1 h) before the test. In conclusion this report shows that Humulus lupulus CO2 extract exerts: (a) a pentobarbital sleep-enhancing property without influencing the motor behavior of rats; (b) an antidepressant activity. The same effects were elicited by the administration of the Humulus lupulus fraction containing α-acids, which can be considered as the major responsible for the enhanced pentobarbital effect and for the antidepressant property.
Paola Zanoli - One of the best experts on this subject based on the ideXlab platform.
-
Increased sexual motivation in female rats treated with Humulus lupulus L. extract.
Journal of Ethnopharmacology, 2011Co-Authors: Vittoria Di Viesti, Manuela Zavatti, Augusta Benelli, Gianluca Carnevale, Paola ZanoliAbstract:Abstract Aim of the study To evaluate the influence of Humulus lupulus extract on sexual behavior in female rats. Materials and methods Ovariectomized rats hormonally primed with estradiol benzoate (1.5 μg/rat) and progesterone (500 μg/rat) were acutely treated by oral gavage with Humulus lupulus extract dosed at 5, 10 and 25 mg/kg and then tested for partner preference and sexual receptivity. Results The administration of Humulus lupulus extract at the highest dose significantly increased the preference for the stimulus male during the partner preference test and the number of proceptive behaviors during the receptivity test, without affecting the lordosis response. Conclusions Humulus lupulus extract increased sexual motivation in hormone-primed female rats.
-
Experimental evidence of the anaphrodisiac activity of Humulus lupulus L. in naïve male rats.
Journal of Ethnopharmacology, 2009Co-Authors: Paola Zanoli, Manuela Zavatti, Augusta Benelli, Marianna Rivasi, Rossella Avallone, Mario BaraldiAbstract:Abstract Ethnopharmacological relevance In the folk medicine Humulus lupulus L. (hops) is mainly recommended as a mild sedative with antispasmodic and digestive properties. It is also reputed to exert an anaphrodisiac effect but it is still lacking the experimental evidence of this activity. Aim of the study To evaluate the influence of Humulus lupulus extract on sexual behavior of both naive and sexually potent male rats; thereafter to investigate the role of 8-prenylnarigenin (8-PN) in the effect displayed by the hop extract. Materials and methods Sprague–Dawley male rats both naive and sexually potent were acutely administered with the hop extract dosed at 5, 10, 25 and 50 mg/kg. In addition the extract was administered daily for 10 consecutive days at the dose of 0.25 mg/kg/day in sexually potent animals. The pure compound 8-PN was acutely administered in naive rats at the dosages of 5, 12.5 and 25 μg/kg. All the animals were screened for their sexual behavior manifestation during the mating test. Results In naive rats the acute administration of Humulus lupulus extract at the doses of 25 and 50 mg/kg significantly reduced the percentage of mounting and ejaculating animals, in comparison to vehicle controls. The other parameters recorded during the mating test were not affected by the hop extract. In sexually potent rats nor the acute neither the repeated administration of the extract modified their copulatory behavior. The pure compound 8-PN failed to influence male sexual behavior of naive rats. Conclusion Humulus lupulus extract exerted an anaphrodisiac effect only in naive rats by inhibiting their mounting and ejaculating behavior. The presence of 8-PN in the extract could be only partially involved in the observed anaphrodisiac effect.
-
pharmacognostic and pharmacological profile of Humulus lupulus l
Journal of Ethnopharmacology, 2008Co-Authors: Paola Zanoli, Manuela ZavattiAbstract:The present review describes the morphological, phytochemical and ethnopharmacological aspects of Humulus lupulus L. (Cannabinaceae) and summarizes the most interesting findings obtained in the preclinical and clinical research related to the plant. The female inflorescences of Humulus lupulus (hops), well-known as bittering agent in brewing industry, have long been used in traditional medicine mainly to treat sleep disturbances. However the sedative activity is still under investigation in order to recognize the active principles responsible for the neuropharmacological effects observed in laboratory animals, and their mechanism of action. Here we report the data from our experiments as well as those obtained from other researchers, focusing on the variability of the results. Other traditional applications of hops as stomachic, antibacterial and antifungal remedy have been supported by in vivo and/or in vitro investigations. In recent years some prenylated chalcones present in hops have received much attention for their biological effects: in particular, xanthohumol has been shown to exert cancer chemopreventive activity in in vitro experiments, while 8-prenylnaringenin has been characterized as one of the most potent phytoestrogens isolated until now. Nevertheless much additional work is needed to open up new biomedical application of these compounds.
-
new insight in the neuropharmacological activity of Humulus lupulus l
Journal of Ethnopharmacology, 2005Co-Authors: Paola Zanoli, Manuela Zavatti, Marianna Rivasi, F Brusiani, Mario BaraldiAbstract:Abstract The purpose of the present study was to investigate the effects of Humulus lupulus CO2 extract and its fraction containing α-acids on the central nervous system of rats. Both tested substances were able to prolong pentobarbital sleeping time, without affecting the latency to the loss of the righting reflex. This effect was dose-dependent, starting from a minimal dose of 10 mg/kg. Neither the extract nor its α-acid fraction affected the locomotor activity in the open field test or exerted an anxiolytic effect in rats submitted to the elevated plus-maze test. Interestingly both compounds reduced the immobility time during the behavioral despair test when administered three times (24, 5 and 1 h) before the test. In conclusion this report shows that Humulus lupulus CO2 extract exerts: (a) a pentobarbital sleep-enhancing property without influencing the motor behavior of rats; (b) an antidepressant activity. The same effects were elicited by the administration of the Humulus lupulus fraction containing α-acids, which can be considered as the major responsible for the enhanced pentobarbital effect and for the antidepressant property.
George S. Morris - One of the best experts on this subject based on the ideXlab platform.
-
Production of trans-β-farnesene by callus of Humulus lupulus
Phytochemistry, 2001Co-Authors: Derek V. Banthorpe, Jacqueline T. Brown, George S. MorrisAbstract:Abstract Callus lines of Humulus lupulus have been established that accumulated trans -β-farnesene as the main component ( ca 14%) of the extractable oil. Incorporation studies with 3 R -[2- 14 C] mevalonate resulted in very significant ( ca 2%) incorporation of the applied tracer into (2 E ,6 E )-farnesol and trans -β-farnesene.
-
Production of trans-β-farnesene by callus of Humulus lupulus
Phytochemistry, 2001Co-Authors: Derek V. Banthorpe, Jacqueline T. Brown, George S. MorrisAbstract:Abstract Callus lines of Humulus lupulus have been established that accumulated trans -β-farnesene as the main component ( ca 14%) of the extractable oil. Incorporation studies with 3 R -[2- 14 C] mevalonate resulted in very significant ( ca 2%) incorporation of the applied tracer into (2 E ,6 E )-farnesol and trans -β-farnesene.
Branka Javornik - One of the best experts on this subject based on the ideXlab platform.
-
New polymorphic dinucleotide and trinucleotide microsatellite loci for hop Humulus lupulus L.
Molecular ecology resources, 2008Co-Authors: Jernej Jakše, Zlata Luthar, Branka JavornikAbstract:One hundred and thirty-five microsatellite markers were developed for hop Humulus lupulus L. from di- and trinucleotide-enriched libraries. Seventy-eight primers showed amplification in two tested genotypes. Twenty-four loci were further characterized on a population of 34 hop samples and the number of alleles per locus, observed heterozygosity and expected heterozygosity ranged from two to 20 (9.7 on average), from 0.0294 to 0.9412 (0.6234 on average) and from 0.0294 to 0.9170 (0.6720 on average), respectively. These microsatellite markers will be further used for studying population structures and relationships and for identifying important qualitative and quantitative loci of hop.
-
molecular phylogeny of wild hops Humulus lupulus l
Heredity, 2006Co-Authors: A Murakami, Branka Javornik, Elisabeth Seigner, Peter Darby, Anton Lutz, M S S Pais, P SvobodaAbstract:We have analysed wild hops collected widely from the Northern Hemisphere, assessing the genetic diversity and the geographical distribution of haplotypes, to investigate the evolution and phylogeny of hops, Humulus lupulus. The haplotypes were characterized by the nuclear ribosomal DNA spacer region (length and DNA sequence) and chloroplast DNA noncoding regions (DNA sequences). The results indicated that primary divergence into European (including Caucasus and Altai hops), and Asian-North American types, was 1.05±0.28 to 1.27±0.30 million years ago. Although an Eastern boundary for European nuclear haplotype distribution was unclear due to the ambiguous origin of Northern Chinese samples, the European hop group showed a wide geographical distribution across Eurasia from the Altai region to Portugal. The low genetic variation in this group suggested rapid and recent expansion. The North American hop group showed high diversity, and is considered to include hops that have migrated from Asia. Japanese and Chinese hops were identified as genetically distinct. This study has shown that wild hops in each growing region are genetically differentiated with considerable genetic diversity. It gives insights into the evolution and domestication of hops that are discussed.
-
Genetic mapping of hop (Humulus lupulus L.) applied to the detection of QTLs for alpha-acid content
Genome, 2006Co-Authors: Andreja Čerenak, Zlatko Satovic, Branka JavornikAbstract:The map locations and effects of quantitative trait loci (QTLs) were estimated for alpha-acid content in hop (Humulus lupulus L.) using amplified fragment length polymorphism (AFLP) and microsatell...
-
Microsatellite DNA Analysis of Wild Hops, Humulus lupulus L.
Genetic Resources and Crop Evolution, 2006Co-Authors: Atsushi Murakami, Branka Javornik, Elisabeth Seigner, Peter Darby, Maria S. S. Pais, Anton Lutz, Petr SvobodaAbstract:To study the relationships and genetic diversity among wild hops, Humulus lupulus, we analyzed 133 samples of wild hops collected from Europe, Asia and North America using polymorphism on 11 microsatellite loci. Although only three primers showed bands in Japanese hops, all other samples showed polymorphic bands at most loci. There were no duplicate genotypes among samples of European, Chinese and North American hops, and each individual hop could be distinguished completely. The phylogenetic tree constructed from DA distance with the UPGMA method showed a large cluster comprised of European hops, although Russian hops from the Caucasus and Altai regions were separate from the European cluster. Chinese and North American samples gave distinct clusters suggesting genetic differentiation. This study has indicated that hop microsatellite DNA is differentiated, and is dependent upon the origin in regions of Europe, Asia and North America.
-
Eleven new microsatellites for hop (Humulus lupulus L.)
Molecular Ecology Notes, 2002Co-Authors: Jernej Jakše, Dunja Bandelj, Branka JavornikAbstract:We present a new set of 11 polymorphic microsatellite primer sequences for use with Humulus lupulus. Microsatellite-enriched libraries for GAn and GTn types of repeats were produced. Sequencing of 72 clones revealed 42 unique inserts containing microsatellites, out of which 19 primer pairs were designed and microsatellite amplification was tested on 39 wild hops and cultivars. Eleven primer pairs showed single locus amplification with 2–13 alleles, average 7.2, of which 17 unique alleles were discovered. One primer pair amplified too strong stutter bands, one locus was monomorphic and multilocus amplification was obtained with the remaining six primer pairs.
Joen Rong Sheu - One of the best experts on this subject based on the ideXlab platform.
-
neuroprotective effects of xanthohumol a prenylated flavonoid from hops Humulus lupulus in ischemic stroke of rats
Journal of Agricultural and Food Chemistry, 2012Co-Authors: Wanjung Lu, Chengying Hsieh, Duensuey Chou, Gongjhe Wu, George Hsiao, Joen Rong SheuAbstract:Xanthohumol is the principal prenylated flavonoid in hops (Humulus lupulus L.), an ingredient of beer. Xanthohumol was found to be a potent chemopreventive agent; however, no data are available con...
