The Experts below are selected from a list of 258 Experts worldwide ranked by ideXlab platform
Chaima Bensaoud - One of the best experts on this subject based on the ideXlab platform.
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proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma Dromedarii
BMC Genomics, 2019Co-Authors: Chaima Bensaoud, Ali Bouattour, Hajer Aounallah, Juliana Mozer Sciani, Fernanda Faria, Ana Marisa Chudzinskitavassi, Youmna MghirbiAbstract:The hard tick Hyalomma Dromedarii is one of the most injurious ectoparasites affecting camels and apparently best adapted to deserts. As long-term blood feeders, ticks are threatened by host defense system compounds that can cause them to be rejected and, ultimately, to die. However, their saliva contains a cocktail of bioactive molecules that enables them to succeed in taking their blood meal. A recent sialotranscriptomic study uncovered the complexity of the salivary composition of the tick H. Dromedarii and provided a database for a proteomic analysis. We carried out a proteomic-informed by transcriptomic (PIT) to identify proteins in salivary glands of both genders of this tick species. We reported the array of 1111 proteins identified in the salivary glands of H. Dromedarii ticks. Only 24% of the proteins were shared by both genders, and concur with the previously described sialotranscriptome complexity. The comparative analysis of the salivary glands of both genders did not reveal any great differences in the number or class of proteins expressed their enzymatic composition or functional classification. Indeed, few proteins in the entire proteome matched those predicted from the transcriptome while others corresponded to other proteins of other tick species. This investigation represents the first proteomic study of H. Dromedarii salivary glands. Our results shed light on the differences between the composition of H. Dromedarii male and female salivary glands, thus enabling us to better understand the gender-specific strategy to feed successfully.
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de novo assembly and annotation of Hyalomma Dromedarii tick acari ixodidae sialotranscriptome with regard to gender differences in gene expression
Parasites & Vectors, 2018Co-Authors: Chaima Bensaoud, Ali Bouattour, Fernanda Faria, Milton Y Nishiyama, Cherif Ben Hamda, Flavio Lichtenstein, Ursula Castro De Oliveira, Inacio L M Junqueiradeazevedo, Kais Ghedira, Youmna MghirbiAbstract:Hard ticks are hematophagous ectoparasites characterized by their long-term feeding. The saliva that they secrete during their blood meal is their crucial weapon against host-defense systems including hemostasis, inflammation and immunity. The anti-hemostatic, anti-inflammatory and immune-modulatory activities carried out by tick saliva molecules warrant their pharmacological investigation. The Hyalomma Dromedarii Koch, 1844 tick is a common parasite of camels and probably the best adapted to deserts of all hard ticks. Like other hard ticks, the salivary glands of this tick may provide a rich source of many compounds whose biological activities interact directly with host system pathways. Female H. Dromedarii ticks feed longer than males, thereby taking in more blood. To investigate the differences in feeding behavior as reflected in salivary compounds, we performed de novo assembly and annotation of H. Dromedarii sialotranscriptome paying particular attention to variations in gender gene expression. The quality-filtered Illumina sequencing reads deriving from a cDNA library of salivary glands led to the assembly of 15,342 transcripts. We deduced that the secreted proteins included: metalloproteases, glycine-rich proteins, mucins, anticoagulants of the mandanin family and lipocalins, among others. Expression analysis revealed differences in the expression of transcripts between male and female H. Dromedarii that might explain the blood-feeding strategies employed by both genders. The annotated sialome of H. Dromedarii helps understand the interaction of tick-host molecules during blood-feeding and can lead to the discovery of new pharmacologically active proteins of ticks of the genus Hyalomma.
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Hyalomma Dromedarii (Acari: Ixodidae) Salivary Gland Extract Inhibits Angiogenesis and Exhibits In Vitro Antitumor Effects.
Journal of Medical Entomology, 2017Co-Authors: Chaima Bensaoud, Zaineb Abdelkafi-koubaa, Hazem Ben Mabrouk, Maram Morjen, Adel Rhim, Issam Hmila, Mohamed El Ayeb, Naziha Marrakchi, Ali Bouattour, Youmna M’ghirbiAbstract:: Hard ticks (Acari: Ixodidae) are blood-sucking ectoparasites characterized by the extended period of their attachment to their host. To access their bloodmeal, ticks secrete saliva containing a range of molecules that target the host's inflammation, immune system, and hemostatic components. Some of these molecules reportedly possess antiangiogenic and antitumor properties. The present study describes our investigation, the first of its kind, of the antiangiogenic and antitumoral effects of the Hyalomma Dromedarii Koch, 1844 (Acari: Ixodidae), salivary gland extract (SGE), which inhibited the adhesion and migration of Human Umbilical Vein Endothelial Cells (HUVECs) in a dose-dependent manner, as well as angiogenesis in the Chick Chorioallantoic Membrane model. Interestingly, H. Dromedarii SGE exerted an antiproliferative effect on U87 glioblastoma cells and inhibited their adhesion and migration to fibrinogen. These results open up new possibilities for characterizing and developing new molecules involved in the key steps of tumor progression.
Ali Bouattour - One of the best experts on this subject based on the ideXlab platform.
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proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma Dromedarii
BMC Genomics, 2019Co-Authors: Chaima Bensaoud, Ali Bouattour, Hajer Aounallah, Juliana Mozer Sciani, Fernanda Faria, Ana Marisa Chudzinskitavassi, Youmna MghirbiAbstract:The hard tick Hyalomma Dromedarii is one of the most injurious ectoparasites affecting camels and apparently best adapted to deserts. As long-term blood feeders, ticks are threatened by host defense system compounds that can cause them to be rejected and, ultimately, to die. However, their saliva contains a cocktail of bioactive molecules that enables them to succeed in taking their blood meal. A recent sialotranscriptomic study uncovered the complexity of the salivary composition of the tick H. Dromedarii and provided a database for a proteomic analysis. We carried out a proteomic-informed by transcriptomic (PIT) to identify proteins in salivary glands of both genders of this tick species. We reported the array of 1111 proteins identified in the salivary glands of H. Dromedarii ticks. Only 24% of the proteins were shared by both genders, and concur with the previously described sialotranscriptome complexity. The comparative analysis of the salivary glands of both genders did not reveal any great differences in the number or class of proteins expressed their enzymatic composition or functional classification. Indeed, few proteins in the entire proteome matched those predicted from the transcriptome while others corresponded to other proteins of other tick species. This investigation represents the first proteomic study of H. Dromedarii salivary glands. Our results shed light on the differences between the composition of H. Dromedarii male and female salivary glands, thus enabling us to better understand the gender-specific strategy to feed successfully.
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de novo assembly and annotation of Hyalomma Dromedarii tick acari ixodidae sialotranscriptome with regard to gender differences in gene expression
Parasites & Vectors, 2018Co-Authors: Chaima Bensaoud, Ali Bouattour, Fernanda Faria, Milton Y Nishiyama, Cherif Ben Hamda, Flavio Lichtenstein, Ursula Castro De Oliveira, Inacio L M Junqueiradeazevedo, Kais Ghedira, Youmna MghirbiAbstract:Hard ticks are hematophagous ectoparasites characterized by their long-term feeding. The saliva that they secrete during their blood meal is their crucial weapon against host-defense systems including hemostasis, inflammation and immunity. The anti-hemostatic, anti-inflammatory and immune-modulatory activities carried out by tick saliva molecules warrant their pharmacological investigation. The Hyalomma Dromedarii Koch, 1844 tick is a common parasite of camels and probably the best adapted to deserts of all hard ticks. Like other hard ticks, the salivary glands of this tick may provide a rich source of many compounds whose biological activities interact directly with host system pathways. Female H. Dromedarii ticks feed longer than males, thereby taking in more blood. To investigate the differences in feeding behavior as reflected in salivary compounds, we performed de novo assembly and annotation of H. Dromedarii sialotranscriptome paying particular attention to variations in gender gene expression. The quality-filtered Illumina sequencing reads deriving from a cDNA library of salivary glands led to the assembly of 15,342 transcripts. We deduced that the secreted proteins included: metalloproteases, glycine-rich proteins, mucins, anticoagulants of the mandanin family and lipocalins, among others. Expression analysis revealed differences in the expression of transcripts between male and female H. Dromedarii that might explain the blood-feeding strategies employed by both genders. The annotated sialome of H. Dromedarii helps understand the interaction of tick-host molecules during blood-feeding and can lead to the discovery of new pharmacologically active proteins of ticks of the genus Hyalomma.
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Hyalomma Dromedarii (Acari: Ixodidae) Salivary Gland Extract Inhibits Angiogenesis and Exhibits In Vitro Antitumor Effects.
Journal of Medical Entomology, 2017Co-Authors: Chaima Bensaoud, Zaineb Abdelkafi-koubaa, Hazem Ben Mabrouk, Maram Morjen, Adel Rhim, Issam Hmila, Mohamed El Ayeb, Naziha Marrakchi, Ali Bouattour, Youmna M’ghirbiAbstract:: Hard ticks (Acari: Ixodidae) are blood-sucking ectoparasites characterized by the extended period of their attachment to their host. To access their bloodmeal, ticks secrete saliva containing a range of molecules that target the host's inflammation, immune system, and hemostatic components. Some of these molecules reportedly possess antiangiogenic and antitumor properties. The present study describes our investigation, the first of its kind, of the antiangiogenic and antitumoral effects of the Hyalomma Dromedarii Koch, 1844 (Acari: Ixodidae), salivary gland extract (SGE), which inhibited the adhesion and migration of Human Umbilical Vein Endothelial Cells (HUVECs) in a dose-dependent manner, as well as angiogenesis in the Chick Chorioallantoic Membrane model. Interestingly, H. Dromedarii SGE exerted an antiproliferative effect on U87 glioblastoma cells and inhibited their adhesion and migration to fibrinogen. These results open up new possibilities for characterizing and developing new molecules involved in the key steps of tumor progression.
Youmna Mghirbi - One of the best experts on this subject based on the ideXlab platform.
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proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma Dromedarii
BMC Genomics, 2019Co-Authors: Chaima Bensaoud, Ali Bouattour, Hajer Aounallah, Juliana Mozer Sciani, Fernanda Faria, Ana Marisa Chudzinskitavassi, Youmna MghirbiAbstract:The hard tick Hyalomma Dromedarii is one of the most injurious ectoparasites affecting camels and apparently best adapted to deserts. As long-term blood feeders, ticks are threatened by host defense system compounds that can cause them to be rejected and, ultimately, to die. However, their saliva contains a cocktail of bioactive molecules that enables them to succeed in taking their blood meal. A recent sialotranscriptomic study uncovered the complexity of the salivary composition of the tick H. Dromedarii and provided a database for a proteomic analysis. We carried out a proteomic-informed by transcriptomic (PIT) to identify proteins in salivary glands of both genders of this tick species. We reported the array of 1111 proteins identified in the salivary glands of H. Dromedarii ticks. Only 24% of the proteins were shared by both genders, and concur with the previously described sialotranscriptome complexity. The comparative analysis of the salivary glands of both genders did not reveal any great differences in the number or class of proteins expressed their enzymatic composition or functional classification. Indeed, few proteins in the entire proteome matched those predicted from the transcriptome while others corresponded to other proteins of other tick species. This investigation represents the first proteomic study of H. Dromedarii salivary glands. Our results shed light on the differences between the composition of H. Dromedarii male and female salivary glands, thus enabling us to better understand the gender-specific strategy to feed successfully.
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de novo assembly and annotation of Hyalomma Dromedarii tick acari ixodidae sialotranscriptome with regard to gender differences in gene expression
Parasites & Vectors, 2018Co-Authors: Chaima Bensaoud, Ali Bouattour, Fernanda Faria, Milton Y Nishiyama, Cherif Ben Hamda, Flavio Lichtenstein, Ursula Castro De Oliveira, Inacio L M Junqueiradeazevedo, Kais Ghedira, Youmna MghirbiAbstract:Hard ticks are hematophagous ectoparasites characterized by their long-term feeding. The saliva that they secrete during their blood meal is their crucial weapon against host-defense systems including hemostasis, inflammation and immunity. The anti-hemostatic, anti-inflammatory and immune-modulatory activities carried out by tick saliva molecules warrant their pharmacological investigation. The Hyalomma Dromedarii Koch, 1844 tick is a common parasite of camels and probably the best adapted to deserts of all hard ticks. Like other hard ticks, the salivary glands of this tick may provide a rich source of many compounds whose biological activities interact directly with host system pathways. Female H. Dromedarii ticks feed longer than males, thereby taking in more blood. To investigate the differences in feeding behavior as reflected in salivary compounds, we performed de novo assembly and annotation of H. Dromedarii sialotranscriptome paying particular attention to variations in gender gene expression. The quality-filtered Illumina sequencing reads deriving from a cDNA library of salivary glands led to the assembly of 15,342 transcripts. We deduced that the secreted proteins included: metalloproteases, glycine-rich proteins, mucins, anticoagulants of the mandanin family and lipocalins, among others. Expression analysis revealed differences in the expression of transcripts between male and female H. Dromedarii that might explain the blood-feeding strategies employed by both genders. The annotated sialome of H. Dromedarii helps understand the interaction of tick-host molecules during blood-feeding and can lead to the discovery of new pharmacologically active proteins of ticks of the genus Hyalomma.
Youmna M’ghirbi - One of the best experts on this subject based on the ideXlab platform.
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Hyalomma Dromedarii (Acari: Ixodidae) Salivary Gland Extract Inhibits Angiogenesis and Exhibits In Vitro Antitumor Effects.
Journal of Medical Entomology, 2017Co-Authors: Chaima Bensaoud, Zaineb Abdelkafi-koubaa, Hazem Ben Mabrouk, Maram Morjen, Adel Rhim, Issam Hmila, Mohamed El Ayeb, Naziha Marrakchi, Ali Bouattour, Youmna M’ghirbiAbstract:: Hard ticks (Acari: Ixodidae) are blood-sucking ectoparasites characterized by the extended period of their attachment to their host. To access their bloodmeal, ticks secrete saliva containing a range of molecules that target the host's inflammation, immune system, and hemostatic components. Some of these molecules reportedly possess antiangiogenic and antitumor properties. The present study describes our investigation, the first of its kind, of the antiangiogenic and antitumoral effects of the Hyalomma Dromedarii Koch, 1844 (Acari: Ixodidae), salivary gland extract (SGE), which inhibited the adhesion and migration of Human Umbilical Vein Endothelial Cells (HUVECs) in a dose-dependent manner, as well as angiogenesis in the Chick Chorioallantoic Membrane model. Interestingly, H. Dromedarii SGE exerted an antiproliferative effect on U87 glioblastoma cells and inhibited their adhesion and migration to fibrinogen. These results open up new possibilities for characterizing and developing new molecules involved in the key steps of tumor progression.
S M Hassan - One of the best experts on this subject based on the ideXlab platform.
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horizontal dispersal and climbing behaviour of the camel tick Hyalomma Dromedarii acari ixodidae in desert ecosystem of northern sudan
Journal of Veterinary Medicine and Animal Production, 2013Co-Authors: A Elghali, S M HassanAbstract:Thirty adults of Hyalomma Dromedarii were released in grass arenas. Dispersal distances of the recaptured ticks were measured every other day for 14 days. Similarly, 5000 larvae and 50 adults of the same tick species were released in each of five metal arenas containing vertically erected sticks. Their diurnal climbing heights were measured at 0600, 1200 and 1800 hours for three successive days. Dispersal of adults in grass ranged between 41 and 144 cm. Adults had no tendency of climbing vegetation. Larvae climbed on the second day for maximum heights of 28.9 and 34.4 cm. They preferred to climb in the afternoon hours to reach maximum heights that ranged between 37.9 and 50.3 cm.
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life cycle of the camel tick Hyalomma Dromedarii acari ixodidae under field conditions in northern sudan
Veterinary Parasitology, 2010Co-Authors: A Elghali, S M HassanAbstract:Engorged Hyalomma Dromedarii females were placed for development in shade and sun (open non-shaded site) in April, August and December for two successive years. Engorged nymphs were also placed at the same sites in January, March, May, June, July and August. Preoviposition periods ranged between 9.8 and 11.7 days in the shade but longer in the sun in December (14.7 days). Egg production index was higher in August (0.72) than in April and December (0.46 and 0.39, respectively) in shade and in August compared to December in the sun (0.65 and 0.29, respectively). In shade and in sun, the prehatching periods were longer in December (50.7 and 48.2 days) than in April and August (25.6 and 29.0 days). Percentage hatchability ranged between 82 to 94.1% and 56.2 and 58.9% in the shade and sun, respectively. Number of eggs laid and eggs hatched were positively correlated with females' engorgement weights. There were high mortality rates of females in the sun and most of the eggs desiccated. Slight increased ambient and soil temperatures increased egg production, shortened preoviposition periods and decreased hatchability while increased humidity shortened preoviposition and prehatching periods and increased hatchability. Nymphal-adult moulting periods were long in January (20.7 days in the shade and 14.4 days in the sun) and short in August in the shade (7.8 days) while they were only 6 days in May and June in the sun. High numbers moulted in all months in the shade (ranges 23.3-29.5 nymphs). The mean mortality of engorged nymphs in the shade was very low. However, in the sun mortality was high (12.9-30 nymphs). Premoulting periods were negatively correlated with ambient temperature but positively correlated with humidity. In the shade, mean number of nymphs moulting increased with increased mean ambient temperature, humidity and rainfall.
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drop off rhythms and survival periods of Hyalomma Dromedarii acari ixodidae fed on camels camelus dromedarius in the sudan
Veterinary Parasitology, 2010Co-Authors: A Elghali, S M HassanAbstract:Flat larvae and adult Hyalomma Dromedarii (Acari: Ixodidae) were fed on camels' ears. Feeding periods and drop-off rhythms of engorged females and nymphs together with engorgement weights of females were recorded. Newly hatched larvae and newly moulted adults were released in nylon mesh bags at the base of a tree. Their survival periods were seasonally monitored. H. Dromedarii behaved exclusively as two-host ticks under field conditions. Larval-nymphal feeding periods ranged between 16 and 27 days according to the season, whereas females fed for 6-9 days. The peak drop-off rhythms of nymphs and females occurred between 18:00 and 20:00 h. Engorgement weights of females at dropping ranged between 0.84 g on day 6 and 0.60 g on day 9 of attachment. Survival duration of the flat adults showed that 18.4%, 5.3% and 4.8% of ticks released in January, April and July, respectively, survived for 1 month. A very few number of ticks survived for extra periods of 3 months, 2 months and 1 month in January, April and July, respectively. Increased air temperature and lower humidity decreased survival duration. Larvae released in February, May, June and August died within 1 week, although they survived under laboratory conditions (35 degrees C and 49-90% Relative Humidity (RH) for 49-60 days.