The Experts below are selected from a list of 318 Experts worldwide ranked by ideXlab platform
William C Faquin - One of the best experts on this subject based on the ideXlab platform.
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Salivary Gland fine needle aspiration and introduction of the milan reporting system
Advances in Anatomic Pathology, 2019Co-Authors: Marc Pusztaszeri, Zubair W Baloch, Esther Diana Rossi, William C FaquinAbstract:Fine needle aspiration (FNA) is a well-established procedure for the diagnosis and management of Salivary Gland lesions despite challenges imposed by their diversity, complexity, and cytomorphologic overlap. Until recently, the reporting of Salivary Gland FNA specimens was inconsistent among different institutions throughout the world, leading to diagnostic confusion among pathologists and clinicians. In 2015, an international group of pathologists initiated the development of an evidence-based tiered classification system for reporting Salivary Gland FNA specimens designated the "Milan System for Reporting Salivary Gland Cytopathology" (MSRSGC) that culminated with the publication of the MSRSGC Atlas in February 2018. The MSRSGC consists of 6 diagnostic categories, which incorporate the morphologic heterogeneity and overlap among various non-neoplastic, benign, and malignant lesions of the Salivary Glands. In addition, each diagnostic category is associated with a risk of malignancy and management recommendations. The main goal of the MSRSGC is to improve communication between cytopathologists and treating clinicians, while also facilitating cytologic-histologic correlation, sharing of data from different laboratories for quality control, and research. Herein, we review the current status of Salivary Gland cytology and the role of MSRSGC in providing a framework for reporting Salivary Gland lesions.
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the milan system for reporting Salivary Gland cytopathology msrsgc an asc iac sponsored system for reporting Salivary Gland fine needle aspiration
Journal of the American Society of Cytopathology, 2018Co-Authors: Esther Diana Rossi, Marc Pusztaszeri, Zubair W Baloch, William C FaquinAbstract:The diagnostic role of Salivary Gland fine-needle aspiration (SG-FNA) is well established in the preoperative evaluation of patients with Salivary Gland lesions. At present, most Salivary SG-FNA specimens are diagnosed based on conventional diagnostic criteria. Nevertheless, there exists a lack of uniform reporting for these specimens to guide the clinical management of patients. This void motivated a group of experienced cytopathologists to spearhead the development of a uniform reporting system. This international panel, under the sponsorship of the American Society of Cytopathology (ASC) and the International Academy of Cytology (IAC), gathered in September 2015 at the European Congress of Cytology, held in Milan, Italy, to propose "The Milan System for Reporting Salivary Gland Cytopathology. This effort sparked the interest of many and brought forth an agreement to develop an evidence-based tiered classification consisting of 6 diagnostic categories. We hope that this standard reporting system will enhance the overall effectiveness of SG-FNA reporting across institutions, with the ultimate result being better communication and improved patient care.
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update in Salivary Gland cytopathology recent molecular advances and diagnostic applications
Seminars in Diagnostic Pathology, 2015Co-Authors: Marc Pusztaszeri, William C FaquinAbstract:Salivary Gland tumors (SGT) are notorious for their extraordinary diversity and for the morphological overlap that exists between many of these entities. Fine-needle aspiration biopsy (FNAB) has a well-established role in the evaluation of patients with a Salivary Gland lesion, helping to guide clinical management. However, Salivary Gland FNAB has several limitations and does not allow for a specific diagnosis in some cases. For these reasons, Salivary Gland FNAB is considered one of the most challenging areas in cytopathology. Over the last decade, new Salivary Gland entities have been recognized, enlarging SGT diversity and complexity even more. In addition, a subset of SGT, including common entities such as pleomorphic adenoma and uncommon new entities such as mammary analog secretory carcinoma, have been characterized cytogenetically by the presence of specific translocations. The molecular consequences of these translocations and their potential prognostic and therapeutic values are not yet well characterized. However, these translocations and their resulting fusion oncogenes and oncoproteins can be used as diagnostic clues in Salivary Gland FNAB material in order to overcome the limitations of cytomorphological evaluation alone. In this review, we focus on SGTs currently known to harbor translocations and fusion genes, including uncommon and recently recognized entities, and discuss their potential application to Salivary Gland FNAB.
Marc Pusztaszeri - One of the best experts on this subject based on the ideXlab platform.
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Salivary Gland fine needle aspiration and introduction of the milan reporting system
Advances in Anatomic Pathology, 2019Co-Authors: Marc Pusztaszeri, Zubair W Baloch, Esther Diana Rossi, William C FaquinAbstract:Fine needle aspiration (FNA) is a well-established procedure for the diagnosis and management of Salivary Gland lesions despite challenges imposed by their diversity, complexity, and cytomorphologic overlap. Until recently, the reporting of Salivary Gland FNA specimens was inconsistent among different institutions throughout the world, leading to diagnostic confusion among pathologists and clinicians. In 2015, an international group of pathologists initiated the development of an evidence-based tiered classification system for reporting Salivary Gland FNA specimens designated the "Milan System for Reporting Salivary Gland Cytopathology" (MSRSGC) that culminated with the publication of the MSRSGC Atlas in February 2018. The MSRSGC consists of 6 diagnostic categories, which incorporate the morphologic heterogeneity and overlap among various non-neoplastic, benign, and malignant lesions of the Salivary Glands. In addition, each diagnostic category is associated with a risk of malignancy and management recommendations. The main goal of the MSRSGC is to improve communication between cytopathologists and treating clinicians, while also facilitating cytologic-histologic correlation, sharing of data from different laboratories for quality control, and research. Herein, we review the current status of Salivary Gland cytology and the role of MSRSGC in providing a framework for reporting Salivary Gland lesions.
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the milan system for reporting Salivary Gland cytopathology msrsgc an asc iac sponsored system for reporting Salivary Gland fine needle aspiration
Journal of the American Society of Cytopathology, 2018Co-Authors: Esther Diana Rossi, Marc Pusztaszeri, Zubair W Baloch, William C FaquinAbstract:The diagnostic role of Salivary Gland fine-needle aspiration (SG-FNA) is well established in the preoperative evaluation of patients with Salivary Gland lesions. At present, most Salivary SG-FNA specimens are diagnosed based on conventional diagnostic criteria. Nevertheless, there exists a lack of uniform reporting for these specimens to guide the clinical management of patients. This void motivated a group of experienced cytopathologists to spearhead the development of a uniform reporting system. This international panel, under the sponsorship of the American Society of Cytopathology (ASC) and the International Academy of Cytology (IAC), gathered in September 2015 at the European Congress of Cytology, held in Milan, Italy, to propose "The Milan System for Reporting Salivary Gland Cytopathology. This effort sparked the interest of many and brought forth an agreement to develop an evidence-based tiered classification consisting of 6 diagnostic categories. We hope that this standard reporting system will enhance the overall effectiveness of SG-FNA reporting across institutions, with the ultimate result being better communication and improved patient care.
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Ancillary Studies for Salivary Gland Cytology
The Milan System for Reporting Salivary Gland Cytopathology, 2018Co-Authors: Marc Pusztaszeri, Fernando Carlos Lander Schmitt, Jorge S. Reis-filho, Marcia EdelweissAbstract:A precise cytological classification of Salivary Gland tumors based on cytomorphology alone is possible for many of the commonly encountered lesions. However, there are challenges for the cytologic diagnosis of some entities. Ancillary tests have become invaluable tools that assist in refining our cytologic diagnoses, and recent advances have improved the diagnostic accuracy of Salivary Gland fine-needle aspiration (FNA), leading to better patient management. A subset of tumors has been characterized cytogenetically by the presence of specific and recurrent translocations. These translocations and their resulting fusion oncogenes and oncoproteins can be used as diagnostic markers in Salivary Gland FNA. In this chapter, we describe the ancillary techniques and several currently available ancillary markers for Salivary Gland FNA.
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update in Salivary Gland cytopathology recent molecular advances and diagnostic applications
Seminars in Diagnostic Pathology, 2015Co-Authors: Marc Pusztaszeri, William C FaquinAbstract:Salivary Gland tumors (SGT) are notorious for their extraordinary diversity and for the morphological overlap that exists between many of these entities. Fine-needle aspiration biopsy (FNAB) has a well-established role in the evaluation of patients with a Salivary Gland lesion, helping to guide clinical management. However, Salivary Gland FNAB has several limitations and does not allow for a specific diagnosis in some cases. For these reasons, Salivary Gland FNAB is considered one of the most challenging areas in cytopathology. Over the last decade, new Salivary Gland entities have been recognized, enlarging SGT diversity and complexity even more. In addition, a subset of SGT, including common entities such as pleomorphic adenoma and uncommon new entities such as mammary analog secretory carcinoma, have been characterized cytogenetically by the presence of specific translocations. The molecular consequences of these translocations and their potential prognostic and therapeutic values are not yet well characterized. However, these translocations and their resulting fusion oncogenes and oncoproteins can be used as diagnostic clues in Salivary Gland FNAB material in order to overcome the limitations of cytomorphological evaluation alone. In this review, we focus on SGTs currently known to harbor translocations and fusion genes, including uncommon and recently recognized entities, and discuss their potential application to Salivary Gland FNAB.
Takashi Tsuji - One of the best experts on this subject based on the ideXlab platform.
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Functional Salivary Gland Regeneration
Methods in molecular biology (Clifton N.J.), 2017Co-Authors: Miho Ogawa, Takashi TsujiAbstract:Oral health and homeostasis are maintained by the functional interactions of many organs, including the Salivary Glands, teeth, and tongue. Salivary Gland dysfunction leads to dry mouth diseases, such as dental caries, bacterial infection, swallowing dysfunction, and reduced quality of life. The current clinical therapies for dry mouth are temporary, and they cannot repair Salivary Gland dysfunction. Salivary Gland regenerative therapy with tissue repair and whole Salivary Gland replacement is a novel organ regenerative therapy. To achieve the recovery of the Salivary Gland function, adult tissue stem cells may be used as a cell source for Salivary Gland tissue repair therapies. To attain the entire Salivary Gland replacement therapy, which represents the next-generation regenerative therapy, we developed a novel cell manipulation method that can regenerate the ectodermal organ germ. The bioengineered Salivary Gland germs successfully engrafted grew in the transplantation site, generating the correct structure. The bioengineered Salivary Glands were able to secrete saliva into the oral cavity and improve dry mouth symptoms. In this chapter, we describe the recent progress and developmental methods for Salivary Gland regeneration therapy.
Matthew P. Hoffman - One of the best experts on this subject based on the ideXlab platform.
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Salivary Gland progenitor cell biology provides a rationale for therapeutic Salivary Gland regeneration.
Oral diseases, 2011Co-Authors: Isabelle M.a. Lombaert, Sarah M. Knox, Matthew P. HoffmanAbstract:An irreversible loss of Salivary Gland function often occurs in humans after removal of Salivary tumors, after therapeutic radiation of head and neck tumors, as a result of Sjogren's syndrome and in genetic syndromes affecting Gland development. The permanent loss of Gland function impairs the oral health of these patients and broadly affects their quality of life. The regeneration of functional Salivary Gland tissue is thus an important therapeutic goal for the field of regenerative medicine and will likely involve stem/progenitor cell biology and/or tissue engineering approaches. Recent reports demonstrate how both innervation of the Salivary Gland epithelium and certain growth factors influence progenitor cell growth during mouse Salivary Gland development. These advances in our understanding suggest that developmental mechanisms of mouse Salivary Gland development may provide a paradigm for postnatal regeneration of both mice and human Salivary Glands. Herein, we will discuss the developmental mechanisms that influence progenitor cell biology and the implications for Salivary Gland regeneration.
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Salivary Gland branching morphogenesis.
Differentiation; research in biological diversity, 2006Co-Authors: Vaishali N. Patel, Ivan T. Rebustini, Matthew P. HoffmanAbstract:Salivary Gland branching morphogenesis involves coordinated cell growth, proliferation, differentiation, migration, apoptosis, and interaction of epithelial, mesenchymal, endothelial, and neuronal cells. The ex vivo analysis of embryonic mouse submandibular Glands, which branch so reproducibly and beautifully in culture, is a powerful tool to investigate the molecular mechanisms regulating epithelium-mesenchyme interactions during development. The more recent analysis of genetically modified mice provides insight into the genetic regulation of branching morphogenesis. The review begins, as did the field historically, focusing on the role of the extracellular matrix (ECM), and its components such as glycosaminoglycans, collagens, and laminins. Following sections describe the modification of the ECM by proteases and the role of cell-matrix and cell-cell receptors. The review then focuses on two major families of growth factors implicated in Salivary Gland development, the fibroblast growth factors (FGFs) and the epidermal growth factors (EGFs). The Salivary Gland phenotypes in mice with genetic modification of FGFs and their receptors highlight the central role of FGFs during Salivary Gland branching morphogenesis. A broader section mentions other molecules implicated from analysis of the phenotypes of genetically modified mice or organ culture experiments. The review concludes with speculation on some future areas of research.
Isabelle M.a. Lombaert - One of the best experts on this subject based on the ideXlab platform.
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Salivary Gland progenitor cell biology provides a rationale for therapeutic Salivary Gland regeneration.
Oral diseases, 2011Co-Authors: Isabelle M.a. Lombaert, Sarah M. Knox, Matthew P. HoffmanAbstract:An irreversible loss of Salivary Gland function often occurs in humans after removal of Salivary tumors, after therapeutic radiation of head and neck tumors, as a result of Sjogren's syndrome and in genetic syndromes affecting Gland development. The permanent loss of Gland function impairs the oral health of these patients and broadly affects their quality of life. The regeneration of functional Salivary Gland tissue is thus an important therapeutic goal for the field of regenerative medicine and will likely involve stem/progenitor cell biology and/or tissue engineering approaches. Recent reports demonstrate how both innervation of the Salivary Gland epithelium and certain growth factors influence progenitor cell growth during mouse Salivary Gland development. These advances in our understanding suggest that developmental mechanisms of mouse Salivary Gland development may provide a paradigm for postnatal regeneration of both mice and human Salivary Glands. Herein, we will discuss the developmental mechanisms that influence progenitor cell biology and the implications for Salivary Gland regeneration.
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Rescue of Salivary Gland function after stem cell transplantation in irradiated Glands.
PloS one, 2008Co-Authors: Isabelle M.a. Lombaert, Jeanette F. Brunsting, Pieter K. Wierenga, Hette Faber, Monique A. Stokman, Tineke Kok, Willy H. Visser, Harm H. Kampinga, Gerald De Haan, Robert P CoppesAbstract:Head and neck cancer is the fifth most common malignancy and accounts for 3% of all new cancer cases each year. Despite relatively high survival rates, the quality of life of these patients is severely compromised because of radiation-induced impairment of Salivary Gland function and consequential xerostomia (dry mouth syndrome). In this study, a clinically applicable method for the restoration of radiation-impaired Salivary Gland function using Salivary Gland stem cell transplantation was developed. Salivary Gland cells were isolated from murine submandibular Glands and cultured in vitro as salispheres, which contained cells expressing the stem cell markers Sca-1, c-Kit and Musashi-1. In vitro, the cells differentiated into Salivary Gland duct cells and mucin and amylase producing acinar cells. Stem cell enrichment was performed by flow cytrometric selection using c-Kit as a marker. In vitro, the cells differentiated into amylase producing acinar cells. In vivo, intra-Glandular transplantation of a small number of c-Kit+ cells resulted in long-term restoration of Salivary Gland morphology and function. Moreover, donor-derived stem cells could be isolated from primary recipients, cultured as secondary spheres and after re-transplantation ameliorate radiation damage. Our approach is the first proof for the potential use of stem cell transplantation to functionally rescue Salivary Gland deficiency.
