The Experts below are selected from a list of 35940 Experts worldwide ranked by ideXlab platform
Christine E Schmidt - One of the best experts on this subject based on the ideXlab platform.
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photocrosslinked Hyaluronic Acid hydrogels natural biodegradable tissue engineering scaffolds
Biotechnology and Bioengineering, 2003Co-Authors: Jennie Baier Leach, Kathryn A. Bivens, Charles W. Patrick, Christine E SchmidtAbstract:Ideally, rationally designed tissue engineering scaffolds promote natural wound healing and regenera- tion. Therefore, we sought to synthesize a biomimetic hydrogel specifically designed to promote tissue repair and chose Hyaluronic Acid (HA; also called hyaluronan) as our initial material. Hyaluronic Acid is a naturally oc- curring polymer associated with various cellular pro- cesses involved in wound healing, such as angiogenesis. Hyaluronic Acid also presents unique advantages: it is easy to produce and modify, hydrophilic and nonadhe- sive, and naturally biodegradable. We prepared a range of glycidyl methacrylate-HA (GMHA) conjugates, which were s ubsequently p hotopolymerized t o f orm crosslinked GMHA hydrogels. A range of hydrogel deg- radation rates was achieved as well as a corresponding, modest range of material properties (e.g., swelling, mesh size). Increased amounts of conjugated methacrylate groups corresponded with increased crosslink densities and decreased degradation rates and yet had an insig- nificant effect on human aortic endothelial cell cytocom- patibility and proliferation. Rat subcutaneous implants of the GMHA hydrogels showed good biocompatibility, little inflammatory response, and similar levels of vascu- larization at the implant edge compared with those of fibrin positive controls. Therefore, these novel GMHA hy- drogels are suitable for modification with adhesive pep- tide sequences (e.g., RGD) and use in a variety of wound- healing applications. © 2003 Wiley Periodicals, Inc. Biotech- nol Bioeng 82: 578-589, 2003.
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Photocrosslinked Hyaluronic Acid hydrogels: Natural, biodegradable tissue engineering scaffolds
Biotechnology and Bioengineering, 2003Co-Authors: Jennie Baier Leach, Kathryn A. Bivens, Charles W. Patrick, Christine E SchmidtAbstract:Ideally, rationally designed tissue engineering scaffolds promote natural wound healing and regeneration. Therefore, we sought to synthesize a biomimetic hydrogel specifically designed to promote tissue repair and chose Hyaluronic Acid (HA; also called hyaluronan) as our initial material. Hyaluronic Acid is a naturally occurring polymer associated with various cellular processes involved in wound healing, such as angiogenesis. Hyaluronic Acid also presents unique advantages: it is easy to produce and modify, hydrophilic and nonadhesive, and naturally biodegradable. We prepared a range of glycidyl methacrylate-HA (GMHA) conjugates, which were subsequently photopolymerized to form crosslinked GMHA hydrogels. A range of hydrogel degradation rates was achieved as well as a corresponding, modest range of material properties (e.g., swelling, mesh size). Increased amounts of conjugated methacrylate groups corresponded with increased crosslink densities and decreased degradation rates and yet had an insignificant effect on human aortic endothelial cell cytocompatibility and proliferation. Rat subcutaneous implants of the GMHA hydrogels showed good biocompatibility, little inflammatory response, and similar levels of vascularization at the implant edge compared with those of fibrin positive controls. Therefore, these novel GMHA hydrogels are suitable for modification with adhesive peptide sequences (e.g., RGD) and use in a variety of wound-healing applications.
Jennie Baier Leach - One of the best experts on this subject based on the ideXlab platform.
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photocrosslinked Hyaluronic Acid hydrogels natural biodegradable tissue engineering scaffolds
Biotechnology and Bioengineering, 2003Co-Authors: Jennie Baier Leach, Kathryn A. Bivens, Charles W. Patrick, Christine E SchmidtAbstract:Ideally, rationally designed tissue engineering scaffolds promote natural wound healing and regenera- tion. Therefore, we sought to synthesize a biomimetic hydrogel specifically designed to promote tissue repair and chose Hyaluronic Acid (HA; also called hyaluronan) as our initial material. Hyaluronic Acid is a naturally oc- curring polymer associated with various cellular pro- cesses involved in wound healing, such as angiogenesis. Hyaluronic Acid also presents unique advantages: it is easy to produce and modify, hydrophilic and nonadhe- sive, and naturally biodegradable. We prepared a range of glycidyl methacrylate-HA (GMHA) conjugates, which were s ubsequently p hotopolymerized t o f orm crosslinked GMHA hydrogels. A range of hydrogel deg- radation rates was achieved as well as a corresponding, modest range of material properties (e.g., swelling, mesh size). Increased amounts of conjugated methacrylate groups corresponded with increased crosslink densities and decreased degradation rates and yet had an insig- nificant effect on human aortic endothelial cell cytocom- patibility and proliferation. Rat subcutaneous implants of the GMHA hydrogels showed good biocompatibility, little inflammatory response, and similar levels of vascu- larization at the implant edge compared with those of fibrin positive controls. Therefore, these novel GMHA hy- drogels are suitable for modification with adhesive pep- tide sequences (e.g., RGD) and use in a variety of wound- healing applications. © 2003 Wiley Periodicals, Inc. Biotech- nol Bioeng 82: 578-589, 2003.
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Photocrosslinked Hyaluronic Acid hydrogels: Natural, biodegradable tissue engineering scaffolds
Biotechnology and Bioengineering, 2003Co-Authors: Jennie Baier Leach, Kathryn A. Bivens, Charles W. Patrick, Christine E SchmidtAbstract:Ideally, rationally designed tissue engineering scaffolds promote natural wound healing and regeneration. Therefore, we sought to synthesize a biomimetic hydrogel specifically designed to promote tissue repair and chose Hyaluronic Acid (HA; also called hyaluronan) as our initial material. Hyaluronic Acid is a naturally occurring polymer associated with various cellular processes involved in wound healing, such as angiogenesis. Hyaluronic Acid also presents unique advantages: it is easy to produce and modify, hydrophilic and nonadhesive, and naturally biodegradable. We prepared a range of glycidyl methacrylate-HA (GMHA) conjugates, which were subsequently photopolymerized to form crosslinked GMHA hydrogels. A range of hydrogel degradation rates was achieved as well as a corresponding, modest range of material properties (e.g., swelling, mesh size). Increased amounts of conjugated methacrylate groups corresponded with increased crosslink densities and decreased degradation rates and yet had an insignificant effect on human aortic endothelial cell cytocompatibility and proliferation. Rat subcutaneous implants of the GMHA hydrogels showed good biocompatibility, little inflammatory response, and similar levels of vascularization at the implant edge compared with those of fibrin positive controls. Therefore, these novel GMHA hydrogels are suitable for modification with adhesive peptide sequences (e.g., RGD) and use in a variety of wound-healing applications.
Charles W. Patrick - One of the best experts on this subject based on the ideXlab platform.
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photocrosslinked Hyaluronic Acid hydrogels natural biodegradable tissue engineering scaffolds
Biotechnology and Bioengineering, 2003Co-Authors: Jennie Baier Leach, Kathryn A. Bivens, Charles W. Patrick, Christine E SchmidtAbstract:Ideally, rationally designed tissue engineering scaffolds promote natural wound healing and regenera- tion. Therefore, we sought to synthesize a biomimetic hydrogel specifically designed to promote tissue repair and chose Hyaluronic Acid (HA; also called hyaluronan) as our initial material. Hyaluronic Acid is a naturally oc- curring polymer associated with various cellular pro- cesses involved in wound healing, such as angiogenesis. Hyaluronic Acid also presents unique advantages: it is easy to produce and modify, hydrophilic and nonadhe- sive, and naturally biodegradable. We prepared a range of glycidyl methacrylate-HA (GMHA) conjugates, which were s ubsequently p hotopolymerized t o f orm crosslinked GMHA hydrogels. A range of hydrogel deg- radation rates was achieved as well as a corresponding, modest range of material properties (e.g., swelling, mesh size). Increased amounts of conjugated methacrylate groups corresponded with increased crosslink densities and decreased degradation rates and yet had an insig- nificant effect on human aortic endothelial cell cytocom- patibility and proliferation. Rat subcutaneous implants of the GMHA hydrogels showed good biocompatibility, little inflammatory response, and similar levels of vascu- larization at the implant edge compared with those of fibrin positive controls. Therefore, these novel GMHA hy- drogels are suitable for modification with adhesive pep- tide sequences (e.g., RGD) and use in a variety of wound- healing applications. © 2003 Wiley Periodicals, Inc. Biotech- nol Bioeng 82: 578-589, 2003.
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Photocrosslinked Hyaluronic Acid hydrogels: Natural, biodegradable tissue engineering scaffolds
Biotechnology and Bioengineering, 2003Co-Authors: Jennie Baier Leach, Kathryn A. Bivens, Charles W. Patrick, Christine E SchmidtAbstract:Ideally, rationally designed tissue engineering scaffolds promote natural wound healing and regeneration. Therefore, we sought to synthesize a biomimetic hydrogel specifically designed to promote tissue repair and chose Hyaluronic Acid (HA; also called hyaluronan) as our initial material. Hyaluronic Acid is a naturally occurring polymer associated with various cellular processes involved in wound healing, such as angiogenesis. Hyaluronic Acid also presents unique advantages: it is easy to produce and modify, hydrophilic and nonadhesive, and naturally biodegradable. We prepared a range of glycidyl methacrylate-HA (GMHA) conjugates, which were subsequently photopolymerized to form crosslinked GMHA hydrogels. A range of hydrogel degradation rates was achieved as well as a corresponding, modest range of material properties (e.g., swelling, mesh size). Increased amounts of conjugated methacrylate groups corresponded with increased crosslink densities and decreased degradation rates and yet had an insignificant effect on human aortic endothelial cell cytocompatibility and proliferation. Rat subcutaneous implants of the GMHA hydrogels showed good biocompatibility, little inflammatory response, and similar levels of vascularization at the implant edge compared with those of fibrin positive controls. Therefore, these novel GMHA hydrogels are suitable for modification with adhesive peptide sequences (e.g., RGD) and use in a variety of wound-healing applications.
Kathryn A. Bivens - One of the best experts on this subject based on the ideXlab platform.
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photocrosslinked Hyaluronic Acid hydrogels natural biodegradable tissue engineering scaffolds
Biotechnology and Bioengineering, 2003Co-Authors: Jennie Baier Leach, Kathryn A. Bivens, Charles W. Patrick, Christine E SchmidtAbstract:Ideally, rationally designed tissue engineering scaffolds promote natural wound healing and regenera- tion. Therefore, we sought to synthesize a biomimetic hydrogel specifically designed to promote tissue repair and chose Hyaluronic Acid (HA; also called hyaluronan) as our initial material. Hyaluronic Acid is a naturally oc- curring polymer associated with various cellular pro- cesses involved in wound healing, such as angiogenesis. Hyaluronic Acid also presents unique advantages: it is easy to produce and modify, hydrophilic and nonadhe- sive, and naturally biodegradable. We prepared a range of glycidyl methacrylate-HA (GMHA) conjugates, which were s ubsequently p hotopolymerized t o f orm crosslinked GMHA hydrogels. A range of hydrogel deg- radation rates was achieved as well as a corresponding, modest range of material properties (e.g., swelling, mesh size). Increased amounts of conjugated methacrylate groups corresponded with increased crosslink densities and decreased degradation rates and yet had an insig- nificant effect on human aortic endothelial cell cytocom- patibility and proliferation. Rat subcutaneous implants of the GMHA hydrogels showed good biocompatibility, little inflammatory response, and similar levels of vascu- larization at the implant edge compared with those of fibrin positive controls. Therefore, these novel GMHA hy- drogels are suitable for modification with adhesive pep- tide sequences (e.g., RGD) and use in a variety of wound- healing applications. © 2003 Wiley Periodicals, Inc. Biotech- nol Bioeng 82: 578-589, 2003.
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Photocrosslinked Hyaluronic Acid hydrogels: Natural, biodegradable tissue engineering scaffolds
Biotechnology and Bioengineering, 2003Co-Authors: Jennie Baier Leach, Kathryn A. Bivens, Charles W. Patrick, Christine E SchmidtAbstract:Ideally, rationally designed tissue engineering scaffolds promote natural wound healing and regeneration. Therefore, we sought to synthesize a biomimetic hydrogel specifically designed to promote tissue repair and chose Hyaluronic Acid (HA; also called hyaluronan) as our initial material. Hyaluronic Acid is a naturally occurring polymer associated with various cellular processes involved in wound healing, such as angiogenesis. Hyaluronic Acid also presents unique advantages: it is easy to produce and modify, hydrophilic and nonadhesive, and naturally biodegradable. We prepared a range of glycidyl methacrylate-HA (GMHA) conjugates, which were subsequently photopolymerized to form crosslinked GMHA hydrogels. A range of hydrogel degradation rates was achieved as well as a corresponding, modest range of material properties (e.g., swelling, mesh size). Increased amounts of conjugated methacrylate groups corresponded with increased crosslink densities and decreased degradation rates and yet had an insignificant effect on human aortic endothelial cell cytocompatibility and proliferation. Rat subcutaneous implants of the GMHA hydrogels showed good biocompatibility, little inflammatory response, and similar levels of vascularization at the implant edge compared with those of fibrin positive controls. Therefore, these novel GMHA hydrogels are suitable for modification with adhesive peptide sequences (e.g., RGD) and use in a variety of wound-healing applications.
Kyung Sun - One of the best experts on this subject based on the ideXlab platform.
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Synthesis and characterization of matrix metalloprotease sensitive-low molecular weight Hyaluronic Acid based hydrogels
Journal of Materials Science: Materials in Medicine, 2008Co-Authors: Jungju Kim, Yongdoo Park, Giyoong Tae, Kyu Back Lee, Soon Jung Hwang, In Sook Kim, Insup Noh, Kyung SunAbstract:Hyaluronic Acid is a naturally derived glycosaminoglycan (GAG) involved in biological processes. A low molecular weight Hyaluronic Acid (50 kDa)-based hydrogel was synthesized using acrylated Hyaluronic Acid. Matrix metalloproteinase (MMP) sensitive Hyaluronic Acid-based hydrogels were prepared by conjugation with two different peptides: cell adhesion peptides containing integrin binding domains (Arg-Gly-Asp: RGD) and a cross-linker with MMP degradable peptides to mimic the remodeling characteristics of natural extracellular matrices (ECMs) by cell-derived MMPs. Mechanical properties of these hydrogels were evaluated with different molecular weights of acrylated Hyaluronic Acid (10 kDa and 50 kDa) cross-linked by MMP sensitive peptides by measuring elastic modulus, viscous modulus, swelling ratio and degradation rate. The MMP sensitive hydrogel based on the 50 kDa Hyaluronic Acid showed a 31.5-fold shorter gelation time, 4.7-fold higher storage modulus and 0.51-fold smaller swelling ratio than those of the hydrogel based on the 10 kDa. Degradation rate was dependent on MMP sensitivity of the peptide cross-linker. MMP sensitive Hyaluronic Acid based hydrogels were degraded faster than MMP insensitive-Hyaluronic Acid-based hydrogels. Human mesenchymal stem cells (MSCs) were cultured in MMP-sensitive or insensitive Hyaluronic Acid-based hydrogels (50 kDa Hyaluronic Acid) and/or immobilized cell adhesive RGD peptides. Cells cultured in the MMP-sensitive hydrogel with RGD peptides showed dramatic cell spreading compared with that of the control, which remained round. This MMP-sensitive low molecular weight Hyaluronic Acid-based hydrogel could be useful in tissue engineering by improving tissue defect regeneration and tissue remodeling.
