The Experts below are selected from a list of 79722 Experts worldwide ranked by ideXlab platform
Adolf Graessmann - One of the best experts on this subject based on the ideXlab platform.
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Breast cancer formation in transgenic animals induced by the whey acidic protein SV40 T antigen (WAP-SV-T) Hybrid Gene.
Oncogene, 1993Co-Authors: Yin-jeh Tzeng, E Guhl, Monika Graessmann, Adolf GraessmannAbstract:After injection of the whey acidic protein (WAP)-SV-T Hybrid Gene into fertilized mouse eggs, eight independent transgenic mouse lines were obtained. Females from three lines developed mammary carcinomas with high frequency, coinciding mostly with lactation. In contrast to the endogenous WAP Gene, expression of the Hybrid Gene continued after lactation. The tumor cells had a very invasive growth characteristic. Tumor regression in vivo was not observed. However, after transfer into tissue culture 25% of the cells ceased to express the Hybrid Gene and acquired the growth characteristic of normal cells. It was possible to retransform these cells by injection of wild-type SV40 DNA, but not after transfer of the Hybrid WAP-SV-T Gene. Inactivation of the endogenous WAP and of the WAP-SV-T transGene did not correlate with DNA methylation.
Yin-jeh Tzeng - One of the best experts on this subject based on the ideXlab platform.
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Breast cancer formation in transgenic animals induced by the whey acidic protein SV40 T antigen (WAP-SV-T) Hybrid Gene.
Oncogene, 1993Co-Authors: Yin-jeh Tzeng, E Guhl, Monika Graessmann, Adolf GraessmannAbstract:After injection of the whey acidic protein (WAP)-SV-T Hybrid Gene into fertilized mouse eggs, eight independent transgenic mouse lines were obtained. Females from three lines developed mammary carcinomas with high frequency, coinciding mostly with lactation. In contrast to the endogenous WAP Gene, expression of the Hybrid Gene continued after lactation. The tumor cells had a very invasive growth characteristic. Tumor regression in vivo was not observed. However, after transfer into tissue culture 25% of the cells ceased to express the Hybrid Gene and acquired the growth characteristic of normal cells. It was possible to retransform these cells by injection of wild-type SV40 DNA, but not after transfer of the Hybrid WAP-SV-T Gene. Inactivation of the endogenous WAP and of the WAP-SV-T transGene did not correlate with DNA methylation.
Daniel W. Pack - One of the best experts on this subject based on the ideXlab platform.
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Intracellular trafficking of Hybrid Gene delivery vectors.
Journal of controlled release : official journal of the Controlled Release Society, 2015Co-Authors: Rahul K. Keswani, Mihael Lazebnik, Daniel W. PackAbstract:Viral and non-viral Gene delivery vectors are in development for human Gene therapy, but both exhibit disadvantages such as inadequate efficiency, lack of cell-specific targeting or safety concerns. We have recently reported the design of Hybrid delivery vectors combining retrovirus-like particles with synthetic polymers or lipids that are efficient, provide sustained Gene expression and are more stable compared to native retroviruses. To guide further development of this promising class of Gene delivery vectors, we have investigated their mechanisms of intracellular trafficking. Moloney murine leukemia virus-like particles (M-VLPs) were complexed with chitosan (Chi) or liposomes (Lip) comprising DOTAP, DOPE and cholesterol to form the Hybrid vectors (Chi/M-VLPs and Lip/M-VLPs, respectively). Transfection efficiency and cellular internalization of the vectors were quantified in the presence of a panel of inhibitors of various endocytic pathways. Intracellular transport and trafficking kinetics of the Hybrid vectors were dependent on the synthetic component and used a combination of clathrin- and caveolar-dependent endocytosis and macropinocytosis. Chi/M-VLPs were slower to transfect compared to Lip/M-VLPs due to the delayed detachment of the synthetic component. The synthetic component of Hybrid Gene delivery vectors plays a significant role in their cellular interactions and processing and is a key parameter for the design of more efficient Gene delivery vehicles.
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effect of serum on transfection by polyethylenimine virus like particle Hybrid Gene delivery vectors
Pharmaceutical Research, 2010Co-Authors: David M Drake, Rahul K. Keswani, Daniel W. PackAbstract:Purpose Murine leukemia virus-like particles (M-VLP) complexed with polymers to promote cellular uptake and endosomal escape represent a new class of effective Gene delivery vectors. Building upon recent studies of viral-synthetic Hybrid vectors, we report the effects of serum on the formation, activity and stability of PEI/M-VLP complexes.
E Guhl - One of the best experts on this subject based on the ideXlab platform.
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Breast cancer formation in transgenic animals induced by the whey acidic protein SV40 T antigen (WAP-SV-T) Hybrid Gene.
Oncogene, 1993Co-Authors: Yin-jeh Tzeng, E Guhl, Monika Graessmann, Adolf GraessmannAbstract:After injection of the whey acidic protein (WAP)-SV-T Hybrid Gene into fertilized mouse eggs, eight independent transgenic mouse lines were obtained. Females from three lines developed mammary carcinomas with high frequency, coinciding mostly with lactation. In contrast to the endogenous WAP Gene, expression of the Hybrid Gene continued after lactation. The tumor cells had a very invasive growth characteristic. Tumor regression in vivo was not observed. However, after transfer into tissue culture 25% of the cells ceased to express the Hybrid Gene and acquired the growth characteristic of normal cells. It was possible to retransform these cells by injection of wild-type SV40 DNA, but not after transfer of the Hybrid WAP-SV-T Gene. Inactivation of the endogenous WAP and of the WAP-SV-T transGene did not correlate with DNA methylation.
Monika Graessmann - One of the best experts on this subject based on the ideXlab platform.
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Breast cancer formation in transgenic animals induced by the whey acidic protein SV40 T antigen (WAP-SV-T) Hybrid Gene.
Oncogene, 1993Co-Authors: Yin-jeh Tzeng, E Guhl, Monika Graessmann, Adolf GraessmannAbstract:After injection of the whey acidic protein (WAP)-SV-T Hybrid Gene into fertilized mouse eggs, eight independent transgenic mouse lines were obtained. Females from three lines developed mammary carcinomas with high frequency, coinciding mostly with lactation. In contrast to the endogenous WAP Gene, expression of the Hybrid Gene continued after lactation. The tumor cells had a very invasive growth characteristic. Tumor regression in vivo was not observed. However, after transfer into tissue culture 25% of the cells ceased to express the Hybrid Gene and acquired the growth characteristic of normal cells. It was possible to retransform these cells by injection of wild-type SV40 DNA, but not after transfer of the Hybrid WAP-SV-T Gene. Inactivation of the endogenous WAP and of the WAP-SV-T transGene did not correlate with DNA methylation.
