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Rosa M. Alonso-salces - One of the best experts on this subject based on the ideXlab platform.
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Polyphenolic profile of butterhead lettuce cultivar by ultrahigh performance liquid chromatography coupled online to UV-visible spectrophotometry and quadrupole time-of-flight mass spectrometry.
Food chemistry, 2018Co-Authors: Gabriela Elena Viacava, Sara I. Roura, Diana M. López-márquez, Luis A. Berrueta, Blanca Gallo, Rosa M. Alonso-salcesAbstract:Abstract In the present study, the butterhead lettuce cultivar was analyzed by ultrahigh performance liquid chromatography (UHPLC) coupled online to diode array detection (DAD), electrospray ionization (ESI) and quadrupole time-of-flight mass spectrometry (QToF/MS) in the positive and negative ion mode in order to characterize its polyphenolic profile for the first time. The instrument acquisition mode MSE was used to collect automatic and simultaneous information of exact mass at high and low collision energies of precursor ions as well as other ions produced as a result of their fragmentation. One hundred eleven phenolic compounds were identified in the acidified hydromethanolic extract of freeze-dried leaves of butterhead lettuce cultivar: 40 hydroxycinnamic acid derivatives, 21 hydroxybenzoic acid derivatives, 2 hydroxyphenylacetic acid derivatives, 18 flavonols, 9 flavones, one flavanone, 7 coumarins, one Hydrolysable Tannin and 12 lignans. Forty-seven of these compounds have been tentatively identified for the first time in lettuce.
Georg Cadisch - One of the best experts on this subject based on the ideXlab platform.
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microbial degradation of Hydrolysable and condensed Tannin polyphenol protein complexes in soils from different land use histories
Soil Biology & Biochemistry, 2007Co-Authors: R Mutabaruka, Kurniatun Hairiah, Georg CadischAbstract:Abstract Polyphenols are capable of binding to proteins and form polyphenol–protein complexes thus reducing the release of N from decomposing plant materials. The objective of this work was to test if under polyphenol-rich vegetations adapted microbial communities had developed capable of breaking down recalcitrant polyphenol–protein complexes. Soils used for this investigation were from different 10-year-old tropical agricultural systems (maize, sugarcane plots and Gliricidia sepium or Peltophorum dasyrrachis woodlots) and natural systems (secondary forest and Imperata cylindrica grassland). TA (tannic acid, Hydrolysable Tannin), QUE (quebracho, condensed Tannin), BSA (bovine serum albumin, protein) or TA/BSA and QUE/BSA polyphenol–protein complexes were incubated at 28 °C in these soils. CO2-C and 13C evolution were periodically monitored and mineral N release, microbial biomass N and phospholipid fatty acid (PLFA) profiles measured at the end. QUE was able to bind about 25% more protein than TA. In all systems the individual uncomplexed substrates were more easily degraded than the complexes. On average, net cumulative CO2-C evolution from TA/BSA complexes was more than 5 times higher than from QUE/BSA complexes, indicating higher C availability and/or lower protection capability of TA compared to QUE. However, net N release was higher from QUE/BSA than from TA/BSA probably due to their higher protein-binding capacity and associated larger degradation of partly unprotected protein as suggested by 13C-CO2 signatures. Microbial respiration patterns indicated that polyphenol complexes were initially degraded more quickly in the maize cropping system than in soils from under polyphenol-rich communities (Peltophorum and natural forest) but this pattern reversed with time. Long-term incubation of QUE/BSA complexes even caused a negative effect on microbial respiration in agricultural soils with low polyphenol contents (e.g. maize and sugarcane). Incubation of polyphenol complexes in soil depressed microbial biomass N in maize, sugarcane, Imperata and forest systems and led to reduced soil pH. However, microbial biomass was increased under the polyphenol-rich vegetation of Peltophorum. The PLFA group 18:2w6,9 was highly enhanced by condensed Tannin–protein complexes additions as compared to control and Hydrolysable polyphenol–protein complexes in soils with high polyphenol contents. Polyphenol complexes increased the fungi:bacteria ratio in systems with a high polyphenol content, particularly with condensed Tannin complexes. The results indicated that systems with a high polyphenol content favoured development of fungal communities that are highly adaptable to phenol-rich soil conditions and high acidity, particularly with regards to the more recalcitrant condensed Tannin–protein complexes.
Gabriela Elena Viacava - One of the best experts on this subject based on the ideXlab platform.
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Polyphenolic profile of butterhead lettuce cultivar by ultrahigh performance liquid chromatography coupled online to UV-visible spectrophotometry and quadrupole time-of-flight mass spectrometry.
Food chemistry, 2018Co-Authors: Gabriela Elena Viacava, Sara I. Roura, Diana M. López-márquez, Luis A. Berrueta, Blanca Gallo, Rosa M. Alonso-salcesAbstract:Abstract In the present study, the butterhead lettuce cultivar was analyzed by ultrahigh performance liquid chromatography (UHPLC) coupled online to diode array detection (DAD), electrospray ionization (ESI) and quadrupole time-of-flight mass spectrometry (QToF/MS) in the positive and negative ion mode in order to characterize its polyphenolic profile for the first time. The instrument acquisition mode MSE was used to collect automatic and simultaneous information of exact mass at high and low collision energies of precursor ions as well as other ions produced as a result of their fragmentation. One hundred eleven phenolic compounds were identified in the acidified hydromethanolic extract of freeze-dried leaves of butterhead lettuce cultivar: 40 hydroxycinnamic acid derivatives, 21 hydroxybenzoic acid derivatives, 2 hydroxyphenylacetic acid derivatives, 18 flavonols, 9 flavones, one flavanone, 7 coumarins, one Hydrolysable Tannin and 12 lignans. Forty-seven of these compounds have been tentatively identified for the first time in lettuce.
Herremans Sophie - One of the best experts on this subject based on the ideXlab platform.
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Effects of Hydrolysable Tannin-treated grass silage on milk yield and composition, nitrogen partitioning and nitrogen isotopic discrimination in lactating dairy cows
'Cambridge University Press (CUP)', 2020Co-Authors: Herremans Sophie, Decruyenaere Virginie, Cantalapiedra-hija G., Eckers Yves, Froidmo EricAbstract:peer reviewedaudience: researcher, professionalThe objective of this study was to evaluate the effects of oak Tannin extract (OTE) added in forage before ensiling on dairy cows fed at 92% of their digestible protein requirements. Six multiparous lactating Holstein cows were used in a crossover design (two treatments × two periods). The control treatment (CON) was based on a diet including 50% of grass silage, whereas the experimental treatment (TAN) included grass silage sprayed with OTE (26 g/kg DM) just before baling. Milk yield (on average 24 kg fat protein corrected milk per day) was not affected, but both milk and rumen fatty acids profiles were impacted by OTE. Nitrogen intake (415 g N per cow per day) and nitrogen use efficiency (NUE; 0.25 on average) were not affected, but a shift from urine (-8% of N intake relatively to control, P = 0.06) to faecal N (+5%; P = 0.004) was observed with the TAN diet (P ≤ 0.05). Nitrogen apparent digestibility was thus reduced for TAN (-3%; P ≤ 0.05). The effect of OTE on ruminal and milk FA profiles suggests an impact on rumen microbiota. Nitrogen isotopic discrimination between animal proteins and diet ("15N) was evaluated as a proxy for NUE. While no differences in NUE were observed across diets, a lower "15N of plasma proteins was found when comparing TAN v. CON diets. This finding supports the concept that "15N would mainly sign the N partitioning at the metabolic level rather than the overall NUE, with the latter also being impacted by digestive processes. Our results agree with a N shift from urine to faeces, and this strategy can thus be adopted to decrease the environmental impact of ruminant protein feeding. © The Animal Consortium 2019
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Effects of Hydrolysable Tannin-treated grass silage on milk yield and composition, nitrogen partitioning and nitrogen isotopic discrimination in lactating dairy cows
2020Co-Authors: Herremans Sophie, Decruyenaere Virginie, Cantalapiedra-hija G., Eckers Yves, Froidmo EricAbstract:The objective of this study was to evaluate the effects of oak Tannin extract (OTE) added in forage before ensiling on dairy cows fed at 92% of their digestible protein requirements. Six multiparous lactating Holstein cows were used in a crossover design (two treatments × two periods). The control treatment (CON) was based on a diet including 50% of grass silage, whereas the experimental treatment (TAN) included grass silage sprayed with OTE (26 g/kg DM) just before baling. Milk yield (on average 24 kg fat protein corrected milk per day) was not affected, but both milk and rumen fatty acids profiles were impacted by OTE. Nitrogen intake (415 g N per cow per day) and nitrogen use efficiency (NUE; 0.25 on average) were not affected, but a shift from urine (-8% of N intake relatively to control, P = 0.06) to faecal N (+5%; P = 0.004) was observed with the TAN diet (P ≤ 0.05). Nitrogen apparent digestibility was thus reduced for TAN (-3%; P ≤ 0.05). The effect of OTE on ruminal and milk FA profiles suggests an impact on rumen microbiota. Nitrogen isotopic discrimination between animal proteins and diet ("15N) was evaluated as a proxy for NUE. While no differences in NUE were observed across diets, a lower "15N of plasma proteins was found when comparing TAN v. CON diets. This finding supports the concept that "15N would mainly sign the N partitioning at the metabolic level rather than the overall NUE, with the latter also being impacted by digestive processes. Our results agree with a N shift from urine to faeces, and this strategy can thus be adopted to decrease the environmental impact of ruminant protein feeding. © The Animal Consortium 2019.Peer reviewe
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Les tanins Hydrolysables sont-ils des additifs d'ensilage prometteurs pour des systèmes laitiers plus respectueux de l'environnement ?
Université de Liège Liège Belgique, 2019Co-Authors: Herremans SophieAbstract:Today, the livestock sector has to address multiple concerns. Being criticized for its environmental impact and competition for land use, a better efficiency at transforming vegetal proteins into animal proteins while limiting food-feed competition is now one of its main challenges. Several strategies have been identified to improve nitrogen use efficiency (NUE) and reduce environment damage of livestock production; the use of plant secondary compounds such as Tannins is one of them. These natural molecules can bind with proteins and protect them against degradation by micro-organisms. Their action can thus help improve nitrogen efficiency and reduce nitrogen losses. Our meta-analysis conducted on 58 experiments showed that Tannins are generally ineffective at improving zootechnical performances but a shift in N excretion was observed, urinary N being reduced in favor of faecal N. However, Hydrolysable Tannins and the effect of Tannins addition before ensiling have been little studied to date, unlike condensed Tannins. This thesis thus aimed at testing the following hypotheses : i) Hydrolysable Tannin extracts can reduce proteolysis both in grass-based silage and rumen, ii) Hydrolysable Tannin extract added before ensiling can improve nitrogen use efficiency in lactating dairy cows. The first experimental results showed that Hydrolysable Tannin extracts were effective at reducing ammonia-nitrogen content of silages suggesting a reduction of proteolysis. NH3-N proportion was reduced by 12 to 18% with oak Tannin and up to 16% with chestnut Tannin. Tannins also decreased ruminal nitrogen degradability of grass silage during enzymatic in vitro trial and in an artificial rumen. Proteolysis reduction thanks to oak Tannin extract linearly increased with Tannin dose in silage. The best dose range for oak and chestnut Tannin extracts in silage seems to be around 30 g/kg of dry matter (DM) of forage. From 50g/kg DM, Tannins showed a detrimental effect on in vitro organic matter digestibility. The second part of the work revealed that oak Tannins extract (added at 26g/kg DM in grass before ensiling) had no effect on nitrogen use efficiency of lactating dairy cows. However, a shift from urine to faecal nitrogen was observed in this trial in presence of oak Tannins. This strategy can thus be adopted to decrease the environmental impact of ruminant protein feeding. This experiment also documented the use of the “nitrogen isotopic discrimination” proxy to compare nitrogen use efficiency of two contrasting diets. The results indicated that the proxy would specifically sign the N partitioning at the metabolic level rather than the overall NUE, the latter also being impacted by digestive processes. The greatest interest of Tannins would thus lie in their positive impact on environment preservation. The addition of Tannin before ensiling seemed pointless in our conditions as compared to direct feeding given that the benefit from protecting proteins in silo did not persist in the rumen. The influence of pH on stability of Hydrolysable Tannin-protein complexes seems contradictory to literature data on condensed Tannins. The specificity of Tannin-protein complexes to both Tannin and protein structures is a great challenge in the understanding of Tannin impacts and the development of Tannin applications in ruminant feeding
Tsutomu Hatano - One of the best experts on this subject based on the ideXlab platform.
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barricyclin d1 a dimeric ellagiTannin with a macrocyclic structure and accompanying Tannins from barringtonia racemosa
Bioscience Biotechnology and Biochemistry, 2021Co-Authors: Shinji Yoshikawa, Tsutomu Hatano, Lih Geeng Chen, Morio Yoshimura, Yoshiaki Amakura, Shoko TaniguchiAbstract:Our examination of high molecular weight polyphenolic constituents in the leaves of Barringtonia racemosa of the family Lecythidaceae uncovered 5 previously undescribed ellagiTannins. One, barringtin M1 (1), among them was a Hydrolysable Tannin monomer, while remaining 4, barringtins D1 (2), D2 (3), D3 (4), and barricyclin D1 (5), were all dimers. Barricyclin D1 had a first macrocyclic structure formed from casuarictin (6) and tellimagrandin I (7), and the other ellagiTannins had structures related to 5. Two additional known phenolics, valoneic acid dilactone (8) and schimawalin A (9), were also isolated from the leaves. These results suggested that the leaves of B. racemosa are a natural resource rich in Hydrolysable Tannin oligomers.
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coriariin m a trimeric Hydrolysable Tannin with dehydrodigalloyl and valoneoyl groups as linking units and accompanying dimeric Hydrolysable Tannins from coriaria japonica
Phytochemistry, 2018Co-Authors: Yuuki Shimozu, Takayasu Hirai, Tsutomu HatanoAbstract:Abstract Three oligomeric Hydrolysable Tannins, coriariins K, L, and M, which were previously undescribed, together with five known Hydrolysable Tannins were isolated from dried leaves of Coriaria japonica. Their structures were determined based on 1D and 2D NMR spectroscopy, HR-ESI-MS, and ECD spectroscopy experiments. Among the isolated compounds, coriariin M has a unique trimer structure where both dehydrodigalloyl and valoneoyl group linkages were found between the Hydrolysable Tannin monomers. Dimeric Hydrolysable Tannins coriariins K and L, having a dehydrodigalloyl group as the linking unit, were structurally related to coriariin A, the main Hydrolysable Tannin of this plant species. Additionally, the complexation of the eight Hydrolysable Tannins isolated in this study with bovine serum albumin (BSA) to form water-soluble macromolecules was analyzed using native polyacrylamide gel electrophoresis (PAGE). A comparison of the behaviors of the oligomeric Hydrolysable Tannins suggested the participation of the hexahydroxydiphenoyl group and the importance of the molecular sizes of the Hydrolysable Tannins in the formation of macromolecules.
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two macrocyclic Hydrolysable Tannin dimers from eugenia uniflora
Phytochemistry, 1997Co-Authors: Saori Nishimoto, Ling-ling Yang, Takashi Yoshida, Tsutomu Hatano, Takuo OkudaAbstract:Abstract Eugeniflorins D1 and D2 new Hydrolysable Tannin dimers, were isolated, together with four known polyphenols, from Eugenia uniflora leaves. Their macrocyclic structures were elucidated from spectral and chemical evidence.
