The Experts below are selected from a list of 90051 Experts worldwide ranked by ideXlab platform
Xin-huai Zhao - One of the best experts on this subject based on the ideXlab platform.
-
Article In Vitro Proliferation and Anti-Apoptosis of the Papain-Generated Casein and Soy Protein Hydrolysates
2016Co-Authors: Towards Osteoblastic Cells, Xiao-wen Pan, Xin-huai ZhaoAbstract:Abstract: Casein and soy protein were digested by papain to three degrees of hydrolysis (DH) 7.3%–13.3%, to obtain respective six casein and soy protein Hydrolysates, aiming to clarify their in vitro proliferation and anti-apoptosis towards a human osteoblastic cell line (hFOB1.19 cells). Six casein and soy protein Hydrolysates at five levels (0.01–0.2 mg/mL) mostly showed proliferation as positive 17β-estradiol did, because they conferred the osteoblasts with cell viability of 100%–114 % and 104%–123%, respectively. The Hydrolysates of higher DH values had stronger proliferation. Casein and soy protein Hydrolysates of the highest DH values altered cell cycle progression, and enhanced cell proportion of S-phase from 50.5 % to 56.5 % and 60.5%. The two also antagonized etoposide- and NaF-induced osteoblast apoptosis. In apoptotic prevention, apoptotic cells were decreased from 31.6 % to 22.6 % and 15.6 % (etoposide treatment), or from 19.5 % to 17.7 % and 12.4 % (NaF treatment), respectively. In apoptotic reversal, soy protein hydrolysate decreased apoptotic cells from 13.3 % to 11.7 % (etoposide treatment), or from 14.5 % to 11.0 % (NaF treatment), but casein hydrolysate showed no reversal effect. It is concluded that the Hydrolysates of two kinds had estradiol-like action on the osteoblasts, and soy protein Hydrolysates had stronge
-
The Cooperative Effect of Genistein and Protein Hydrolysates on the Proliferation and Survival of Osteoblastic Cells (hFOB 1.19)
MDPI AG, 2016Co-Authors: Shuo Wang, Xin-huai ZhaoAbstract:Chum salmon skin gelatin, de-isoflavoned soy protein, and casein were hydrolyzed at two degrees of hydrolysis. Genistein, the prepared Hydrolysates, and genistein-hydrolysate combinations were assessed for their proliferative and anti-apoptotic effects on human osteoblasts (hFOB 1.19) to clarify potential cooperative effects between genistein and these Hydrolysates in these two activities. Genistein at 2.5 μg/L demonstrated the highest proliferative activity, while the higher dose of genistein inhibited cell growth. All Hydrolysates promoted osteoblast proliferation by increasing cell viability to 102.9%–131.1%. Regarding etoposide- or NaF-induced osteoblast apoptosis, these Hydrolysates at 0.05 g/L showed both preventive and therapeutic effects against apoptosis. In the mode of apoptotic prevention, the Hydrolysates decreased apoptotic cells from 32.9% to 15.2%–23.7% (etoposide treatment) or from 23.6% to 14.3%–19.6% (NaF treatment). In the mode of apoptotic rescue, the Hydrolysates lessened the extent of apoptotic cells from 15.9% to 13.0%–15.3% (etoposide treatment) or from 13.3% to 10.9%–12.7% (NaF treatment). Gelatin Hydrolysates showed the highest activities among all Hydrolysates in all cases. All investigated combinations (especially the genistein-gelatin hydrolysate combination) had stronger proliferation, apoptotic prevention, and rescue than genistein itself or their counterpart Hydrolysates alone, suggesting that genistein cooperated with these Hydrolysates, rendering greater activities in osteoblast proliferation and anti-apoptosis
-
In Vitro Proliferation and Anti-Apoptosis of the Papain-Generated Casein and Soy Protein Hydrolysates towards Osteoblastic Cells (hFOB1.19)
International journal of molecular sciences, 2015Co-Authors: Xiao-wen Pan, Xin-huai ZhaoAbstract:Casein and soy protein were digested by papain to three degrees of hydrolysis (DH) 7.3%-13.3%, to obtain respective six casein and soy protein Hydrolysates, aiming to clarify their in vitro proliferation and anti-apoptosis towards a human osteoblastic cell line (hFOB1.19 cells). Six casein and soy protein Hydrolysates at five levels (0.01-0.2 mg/mL) mostly showed proliferation as positive 17β-estradiol did, because they conferred the osteoblasts with cell viability of 100%-114% and 104%-123%, respectively. The Hydrolysates of higher DH values had stronger proliferation. Casein and soy protein Hydrolysates of the highest DH values altered cell cycle progression, and enhanced cell proportion of S-phase from 50.5% to 56.5% and 60.5%. The two also antagonized etoposide- and NaF-induced osteoblast apoptosis. In apoptotic prevention, apoptotic cells were decreased from 31.6% to 22.6% and 15.6% (etoposide treatment), or from 19.5% to 17.7% and 12.4% (NaF treatment), respectively. In apoptotic reversal, soy protein hydrolysate decreased apoptotic cells from 13.3% to 11.7% (etoposide treatment), or from 14.5% to 11.0% (NaF treatment), but casein hydrolysate showed no reversal effect. It is concluded that the Hydrolysates of two kinds had estradiol-like action on the osteoblasts, and soy protein Hydrolysates had stronger proliferation and anti-apoptosis on the osteoblasts than casein Hydrolysates.
-
In Vitro Calcium‐Chelating and Platelet Anti‐Aggregation Activities of Soy Protein Hydrolysate Modified by the Alcalase‐Catalyzed Plastein Reaction
Journal of Food Biochemistry, 2014Co-Authors: Mei‐ling Zhang, Xin-huai ZhaoAbstract:Soy protein hydrolysate (SPH) (1 mg/mL), generated by alcalase, had a calcium-chelating activity of 41 mg/g peptide. The alcalase-catalyzed plastein reaction of SPH was carried out in different media: ethanol–water, methanol–water and water. The effects of SPH and of resulting modified Hydrolysates on calcium-chelating activities and inhibition of calcium precipitation and platelet aggregation were assessed in vitro. The optimum ethanol concentration, alcalase level and reaction temperature for the plastein reaction was 56.8% v/v, 5.26 kU/g peptide and 33.1C, respectively. The modified Hydrolysates had better calcium-chelating activities and stronger inhibition of calcium precipitation and platelet aggregation than SPH. The modified hydrolysate with the highest calcium-chelating activity showed the strongest inhibitory effects on platelet aggregation and calcium precipitation. The plastein reaction enhances the in vitro calcium-chelating and platelet anti-aggregation activities of SPH. Practical Applications In this study, an alcalase-catalyzed plastein reaction carried out in three media (ethanol–water, methanol–water and water) was used to modify the in vitro calcium-chelating and platelet anti-aggregation activities of an alcalase-generated soy protein hydrolysate. The results revealed that the modified Hydrolysates of higher calcium-chelating activity had stronger inhibitory effects on platelet aggregation and calcium precipitation. The results of this study provide further insight on the enzymatic synthesis of bioactive peptides of higher activity using the plastein reaction.
Venkateswar L Rao - One of the best experts on this subject based on the ideXlab platform.
-
xylitol production from corn fiber and sugarcane bagasse Hydrolysates by candida tropicalis
Bioresource Technology, 2006Co-Authors: Sreenivas R Rao, Ch Pavana Jyothi, R S Prakasham, P N Sarma, Venkateswar L RaoAbstract:A natural isolate, Candida tropicalis was tested for xylitol production from corn fiber and sugarcane bagasse Hydrolysates. Fermentation of corn fiber and sugarcane bagasse hydrolysate showed xylose uptake and xylitol production, though these were very low, even after hydrolysate neutralization and treatments with activated charcoal and ion exchange resins. Initial xylitol production was found to be 0.43 g/g and 0.45 g/g of xylose utilised with corn fiber and sugarcane bagasse hydrolysate respectively. One of the critical factors for low xylitol production was the presence of inhibitors in these Hydrolysates. To simulate influence of hemicellulosic sugar composition on xylitol yield, three different combinations of mixed sugar control experiments, without the presence of any inhibitors, have been performed and the strain produced 0.63 g/g, 0.68 g/g and 0.72 g/g of xylose respectively. To improve yeast growth and xylitol production with these Hydrolysates, which contain inhibitors, the cells were adapted by sub culturing in the hydrolysate containing medium for 25 cycles. After adaptation the organism produced more xylitol 0.58 g/g and 0.65 g/g of xylose with corn fiber hydrolysate and sugarcane bagasse hydrolysate respectively.
M C Gomezguillen - One of the best experts on this subject based on the ideXlab platform.
-
squid gelatin Hydrolysates with antihypertensive anticancer and antioxidant activity
Food Research International, 2011Co-Authors: A Aleman, M C Gomezguillen, E Perezsantin, S Bordenavejuchereau, I Arnaudin, P MonteroAbstract:Abstract Gelatin obtained from giant squid (Dosidicus gigas) inner and outer tunics was hydrolyzed by seven commercial proteases (Protamex, Trypsin, Neutrase, Savinase, NS37005, Esperase and Alcalase) to produce bioactive Hydrolysates. The Alcalase hydrolysate was the most potent angiotensin-converting enzyme (ACE) inhibitor (IC50 = 0.34 mg/mL) while the Esperase hydrolysate showed the highest cytotoxic effect on cancer cells, with IC50 values of 0.13 and 0.10 mg/mL for MCF-7 (human breast carcinoma) and U87 (glioma) cell lines, respectively. The radical scavenging capacity of gelatin increased approximately 3-fold for Protamex, Neutrase and NS37005 Hydrolysates and between 7 and 10-fold for Trypsin, Savinase, Esperase and Alcalase Hydrolysates. Trypsin, Savinase, Esperase and Alcalase Hydrolysates had a metal chelating capacity above 80% whereas Protamex, Neutrase and NS37005 Hydrolysates registered less than 25%. The antioxidant activity measured by FRAP (ferric ion reducing power) was largely unaffected by the enzyme used, increasing approximately 2-fold for all Hydrolysates. The most active Hydrolysates (Alcalase and Esperase) were comprised mostly of peptides with molecular weights ranging from 500 to 1400 Da, however, a clear relationship between bioactive properties and molecular weight distribution of all the Hydrolysates was not fully established.
-
antioxidant and functional properties of gelatin Hydrolysates obtained from skin of sole and squid
Food Chemistry, 2009Co-Authors: B Gimenez, P Montero, A Aleman, M C GomezguillenAbstract:Abstract Antioxidant and functional properties were evaluated for gelatin Hydrolysates obtained from sole and squid skin gelatin by Alcalase, with a degree of hydrolysis of ∼35% and ∼50%, respectively. Both Hydrolysates mainly consisted of peptides below 6.5 kDa, together with peptidic material from around 16 to 6.5 kDa. Moreover, the squid hydrolysate showed a peptide band of around 26 kDa. Antioxidant properties of both gelatins were highly increased by hydrolysis, especially ABTS and metal chelating abilities. The squid hydrolysate showed the highest antioxidant capacity by FRAP, ABTS and metal chelating assays in spite of the lower content in hydrophobic amino acids. Both gelatin Hydrolysates had a good solubility (over 95%). The emulsifying activity index (EAI) decreased with increasing concentration. Conversely, the foam expansion increased with increasing concentration. However, both foam and emulsion stabilities were not apparently affected by the concentration of hydrolysate. In the case of the sole hydrolysate, which showed a lower degree Pro and Lys hydroxylation, foam stability was very poor, and 50% of foam expansion was lost after 5 min at all concentrations.
Fereidoon Shahidi - One of the best experts on this subject based on the ideXlab platform.
-
functionalities and antioxidant properties of protein Hydrolysates from the muscle of ornate threadfin bream treated with pepsin from skipjack tuna
Food Chemistry, 2011Co-Authors: Sitthipong Nalinanon, Soottawat Benjakul, Hideki Kishimura, Fereidoon ShahidiAbstract:Functional properties and antioxidant activities of protein Hydrolysates prepared from ornate threadfin bream (Nemipterus hexodon) muscle, using skipjack tuna pepsin, with different degrees of hydrolysis (DH: 10%, 20% and 30%), were evaluated. Emulsifying and foaming properties of Hydrolysates were governed by their DH and concentrations used. Hydrolysates with 20% DH had the highest scavenging activities for ABTS and DPPH radicals. However, chelating activity of Hydrolysates for ferrous ion increased as DH increased. Size exclusion chromatography of the hydrolysate with 20% DH using Sephadex G-25 revealed that antioxidative peptides with molecular weight of approximately 1.3 kDa exhibited the highest ABTS radical-scavenging activity. In vitro simulated gastrointestinal digestion indicated that ABTS radical-scavenging activity of the antioxidative peptides was not affected by pepsin hydrolysis, whilst further digestion by pancreatin enhanced the activity. Therefore, protein hydrolysate from the muscle of ornate threadfin bream produced by skipjack tuna pepsin can be used as a promising source of functional peptides with antioxidant properties.
-
antioxidant activity and water holding capacity of canola protein Hydrolysates
Food Chemistry, 2008Co-Authors: Nichole Cumby, Ying Zhong, M Naczk, Fereidoon ShahidiAbstract:Abstract Canola protein Hydrolysates were prepared using commercial enzymes, namely Alcalase, an endo -peptidase and Flavourzyme with both endo - and exo -peptidase activities. The Hydrolysates so prepared were effective as antioxidants in model systems, mainly by scavenging of free radicals and acting as reducing agents. This effect was concentration-dependent and also influenced by the type of enzyme employed in the process. The hydrolysate prepared using flavourzyme showed the highest antioxidant activity among all samples, whereas the Hydrolysates prepared by combination of Alcalase and Flavourzyme did not differ significantly ( P > 0.05) in antioxidant effectiveness from that produced by Alcalase alone. The Hydrolysates were also found to be effective in enhancing water-holding capacity and cooking yield in a meat model system. Their capability in improving the cooking yield of meat was in the order of Flavourzyme Hydrolysates > combination Hydrolysates > Alcalase Hydrolysates. These results suggest that canola protein Hydrolysates can be useful in terms of their functionality and as functional food ingredients and that their composition determines their functional properties and thus their potential application in the food and feed industries.
Namhyun Chung - One of the best experts on this subject based on the ideXlab platform.
-
Influence of mixed protein Hydrolysates on the growth and viability of chinese hamster ovary cells
Journal of the Korean Society for Applied Biological Chemistry, 2009Co-Authors: Ji Young Lee, Yong Kwon Lee, Bok-hwan Chun, Jang Han Lee, Jason Ahn, Namhyun ChungAbstract:Plant-derived protein Hydrolysates have been considered promising substitutes for serum in mammalian cell growth cultures. We selected a few growth promoting protein Hydrolysates (Broadbean, Soy F, and Soy P) and made mixture compositions with those Hydrolysates, which considerably improved the growth and viability of Chinese hamster ovary (CHO) cells. We also investigated the effects of both individual ultrafiltered fraction of protein Hydrolysates and mixture composition with the fractionated Hydrolysates, and found that some of the mixture compositions containing fractionated hydrolysate with a small fraction of a high-molecular-weight hydrolysate supported a cumulative cell count equal to or slightly greater than that observed in the control media containing unfractionated Hydrolysates. It is believed that such medium composition is beneficial for downstream processing of protein production due to a cleaner broth. We conclude that CHO cell growth and viability may be improved in media containing a mixture of unfractionated or fractionated plant protein Hydrolysates compared with media containing a single hydrolysate.
-
Influence of soy protein Hydrolysates on the differentiation progress of human keratinocytes in culture
Journal of the Korean Society for Applied Biological Chemistry, 2009Co-Authors: Yong Kwon Lee, Bok-hwan Chun, Namhyun ChungAbstract:To evaluate the effect of non-animal protein Hydrolysates on keratinocyte differentiation, commercially-available soy protein Hydrolysates were employed as feed supplements for serum-free culture medium in the absence or presence of bovine pituitary extract (BPE). Supplementation of keratinocyte culture medium containing BPE with Soy Hydrolysate (1 g/L) or Bacto Soytone (1 or 2 g/L) inhibited the mRNA and protein expression levels of early and late differentiation markers, and also helped cells to maintain a small size, an indication of an undifferentiated state. These results show that the supplementation of protein Hydrolysates might be helpful to delay keratinocytes differentiation.
-
Usability of size-excluded fractions of soy protein Hydrolysates for growth and viability of Chinese hamster ovary cells in protein-free suspension culture.
Bioresource technology, 2006Co-Authors: Bok-hwan Chun, Jong Hwang Kim, Hojoung Lee, Namhyun ChungAbstract:To investigate the effect of size-excluded fraction of non-animal protein hydrolysate on growth, viability and longevity of Chinese hamster ovary (CHO) cells, several commercially available protein Hydrolysates were evaluated as a feed supplement to chemically-defined protein-free suspension culture. Soy protein Hydrolysates showed better supporting capability for cell growth and viability than the other types of Hydrolysates. Maximal cell growth was not affected greatly by size exclusion of some soy Hydrolysates such as bacto soytone and soy Hydrolysates. CHO cells supplemented with size-excluded fractions of the two Hydrolysates showed viable cell density and viability almost equal to those with their crude Hydrolysates, although soy Hydrolysates showed a little better performance. This suggested that the size-excluded hydrolysate fractions of some soy hydrolysate might be a potential culture medium additive to achieve better downstream operation in a large-scale production as well as enhanced productivity.
