The Experts below are selected from a list of 63 Experts worldwide ranked by ideXlab platform
M. Rolando - One of the best experts on this subject based on the ideXlab platform.
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forecasting of Hydrophilic Contact Lens tolerance by means of tear ferning test
Graefes Archive for Clinical and Experimental Ophthalmology, 1998Co-Authors: Luigi Ravazzoni, Carlo Ghini, Angelo Macrì, M. RolandoAbstract:· Background: The ability of the tear ferning test to predict future Hydrophilic Contact Lens tolerance was studied. · Methods: The tear ferning test (TFT) was performed on one randomly chosen eye of each of the 116 subjects who came to our Contact Lens clinic for Hydrophilic Contact Lens application. The TFT was performed at the time of enrollment (T0) and then 1 month (T1), 3 months (T2) and 6 months (T3) after Contact Lens fitting. The specificity and the sensitivity of the TFT in identifying future Contact Lens tolerance was then studied. The statistical significance of the differences in behavior through the study period among the subjects with different pre-fitting TFT results was evaluated by means of survival curves. · Results: When only type I ferning was considered as a marker of good tear film conditions, the ability of the TFT to forecast Contact Lens tolerance had 78.95% sensitivity, 78.35% specificity and 78.45% diagnostic precision. The TFT showed sensitivity of 100%, specificity of 86.6% and diagnostic precision of 97.4% when performed after 1 month of Contact Lens wearing (T1). Survival curve analysis showed a statistically significant difference in behavior between the group of subjects with pre-fitting ferning type I and the other three groups (P<0.001). · Conclusion: The TFT appears to have good sensitivity and specific for prediction of Contact Lens tolerance in a clinical setting.
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Forecasting of Hydrophilic Contact Lens tolerance by means of tear ferning test
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 1998Co-Authors: Luigi Ravazzoni, Carlo Ghini, Angelo Macrì, M. RolandoAbstract:The ability of the tear ferning test to predict future Hydrophilic Contact Lens tolerance was studied. The tear ferning test (TFT) was performed on one randomly chosen eye of each of the 116 subjects who came to our Contact Lens clinic for Hydrophilic Contact Lens application. The TFT was performed at the time of enrollment (TO) and then 1 month (T1), 3 months (T2) and 6 months (T3) after Contact Lens fitting. The specificity and the sensitivity of the TFT in identifying future Contact Lens tolerance was then studied. The statistical significance of the differences in behavior through the study period among the subjects with different pre-fitting TFT results was evaluated by means of survival curves. When only type I ferning was considered as a marker of good tear film conditions, the ability of the TFT to forecast Contact Lens tolerance had 78.95% sensitivity, 78.35% specificity and 78.45% diagnostic precision. The TFT showed sensitivity of 100%, specificity of 86.6% and diagnostic precision of 97.4% when performed after 1 month of Contact Lens wearing (T1). Survival curve analysis showed a statistically significant difference in behavior between the group of subjects with pre-fitting ferning type I and the other three groups (P < 0.001). The TFT appears to have good sensitivity and specific for prediction of Contact Lens tolerance in a clinical setting.
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Forecasting of Hydrophilic Contact Lens tolerance by means of tear ferning test
Graefe's Archive for Clinical and Experimental Ophthalmology, 1998Co-Authors: Luigi Ravazzoni, Carlo Ghini, Angelo Macrì, M. RolandoAbstract:· Background: The ability of the tear ferning test to predict future Hydrophilic Contact Lens tolerance was studied. · Methods: The tear ferning test (TFT) was performed on one randomly chosen eye of each of the 116 subjects who came to our Contact Lens clinic for Hydrophilic Contact Lens application. The TFT was performed at the time of enrollment (T0) and then 1 month (T1), 3 months (T2) and 6 months (T3) after Contact Lens fitting. The specificity and the sensitivity of the TFT in identifying future Contact Lens tolerance was then studied. The statistical significance of the differences in behavior through the study period among the subjects with different pre-fitting TFT results was evaluated by means of survival curves. · Results: When only type I ferning was considered as a marker of good tear film conditions, the ability of the TFT to forecast Contact Lens tolerance had 78.95% sensitivity, 78.35% specificity and 78.45% diagnostic precision. The TFT showed sensitivity of 100%, specificity of 86.6% and diagnostic precision of 97.4% when performed after 1 month of Contact Lens wearing (T1). Survival curve analysis showed a statistically significant difference in behavior between the group of subjects with pre-fitting ferning type I and the other three groups ( P
Luigi Ravazzoni - One of the best experts on this subject based on the ideXlab platform.
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forecasting of Hydrophilic Contact Lens tolerance by means of tear ferning test
Graefes Archive for Clinical and Experimental Ophthalmology, 1998Co-Authors: Luigi Ravazzoni, Carlo Ghini, Angelo Macrì, M. RolandoAbstract:· Background: The ability of the tear ferning test to predict future Hydrophilic Contact Lens tolerance was studied. · Methods: The tear ferning test (TFT) was performed on one randomly chosen eye of each of the 116 subjects who came to our Contact Lens clinic for Hydrophilic Contact Lens application. The TFT was performed at the time of enrollment (T0) and then 1 month (T1), 3 months (T2) and 6 months (T3) after Contact Lens fitting. The specificity and the sensitivity of the TFT in identifying future Contact Lens tolerance was then studied. The statistical significance of the differences in behavior through the study period among the subjects with different pre-fitting TFT results was evaluated by means of survival curves. · Results: When only type I ferning was considered as a marker of good tear film conditions, the ability of the TFT to forecast Contact Lens tolerance had 78.95% sensitivity, 78.35% specificity and 78.45% diagnostic precision. The TFT showed sensitivity of 100%, specificity of 86.6% and diagnostic precision of 97.4% when performed after 1 month of Contact Lens wearing (T1). Survival curve analysis showed a statistically significant difference in behavior between the group of subjects with pre-fitting ferning type I and the other three groups (P<0.001). · Conclusion: The TFT appears to have good sensitivity and specific for prediction of Contact Lens tolerance in a clinical setting.
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Forecasting of Hydrophilic Contact Lens tolerance by means of tear ferning test
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 1998Co-Authors: Luigi Ravazzoni, Carlo Ghini, Angelo Macrì, M. RolandoAbstract:The ability of the tear ferning test to predict future Hydrophilic Contact Lens tolerance was studied. The tear ferning test (TFT) was performed on one randomly chosen eye of each of the 116 subjects who came to our Contact Lens clinic for Hydrophilic Contact Lens application. The TFT was performed at the time of enrollment (TO) and then 1 month (T1), 3 months (T2) and 6 months (T3) after Contact Lens fitting. The specificity and the sensitivity of the TFT in identifying future Contact Lens tolerance was then studied. The statistical significance of the differences in behavior through the study period among the subjects with different pre-fitting TFT results was evaluated by means of survival curves. When only type I ferning was considered as a marker of good tear film conditions, the ability of the TFT to forecast Contact Lens tolerance had 78.95% sensitivity, 78.35% specificity and 78.45% diagnostic precision. The TFT showed sensitivity of 100%, specificity of 86.6% and diagnostic precision of 97.4% when performed after 1 month of Contact Lens wearing (T1). Survival curve analysis showed a statistically significant difference in behavior between the group of subjects with pre-fitting ferning type I and the other three groups (P < 0.001). The TFT appears to have good sensitivity and specific for prediction of Contact Lens tolerance in a clinical setting.
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Forecasting of Hydrophilic Contact Lens tolerance by means of tear ferning test
Graefe's Archive for Clinical and Experimental Ophthalmology, 1998Co-Authors: Luigi Ravazzoni, Carlo Ghini, Angelo Macrì, M. RolandoAbstract:· Background: The ability of the tear ferning test to predict future Hydrophilic Contact Lens tolerance was studied. · Methods: The tear ferning test (TFT) was performed on one randomly chosen eye of each of the 116 subjects who came to our Contact Lens clinic for Hydrophilic Contact Lens application. The TFT was performed at the time of enrollment (T0) and then 1 month (T1), 3 months (T2) and 6 months (T3) after Contact Lens fitting. The specificity and the sensitivity of the TFT in identifying future Contact Lens tolerance was then studied. The statistical significance of the differences in behavior through the study period among the subjects with different pre-fitting TFT results was evaluated by means of survival curves. · Results: When only type I ferning was considered as a marker of good tear film conditions, the ability of the TFT to forecast Contact Lens tolerance had 78.95% sensitivity, 78.35% specificity and 78.45% diagnostic precision. The TFT showed sensitivity of 100%, specificity of 86.6% and diagnostic precision of 97.4% when performed after 1 month of Contact Lens wearing (T1). Survival curve analysis showed a statistically significant difference in behavior between the group of subjects with pre-fitting ferning type I and the other three groups ( P
Jerry Rapp - One of the best experts on this subject based on the ideXlab platform.
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Protein-lipid interaction on the surface of a Hydrophilic Contact Lens in vitro.
Current eye research, 1997Co-Authors: Andrew R. Bontempo, Jerry RappAbstract:To investigate the mechanism of protein-lipid interactions responsible for biofilm formation on the surface of Hydrophilic Contact Lenses in vitro. New, never-worn Hydrophilic Contact Lenses were individually incubated in a protein-only, lipid-only, or combination protein-lipid artificial tear solution for 24 hours at 37 degrees C with constant stirring. Deposited lipids were removed with a methanol based extraction procedure, separated using high-performance, thin layer chromatography and quantitatively analyzed densitometrically. Deposited proteins were extracted with 4M urea, separated using gel electrophoresis and quantitatively analyzed densitometrically. The presence of protein in the artificial tear solution has a profound effect on the nature of lipid deposition for each group of Hydrophilic Lens, whereas the presence of lipid has a significant effect on the nature of protein deposition for only a group IV Lens. In addition, the presence of lipid deposits on a group IV Lens decreases the adsorption of lysozyme, while the presence of protein deposits reduces the amount of total lipid adhering to a group II Lens. Protein adsorption on a group IV Lens renders the Lens surface less Hydrophilic and, thereby, more susceptible to lipid deposition, which in turn increases surface hydrophobicity and inhibits additional protein deposition. For a group II Lens, positively charged protein competes with and replaces some of the polar lipids attached to the Lens. Thus, the interaction of protein and lipid on a Lens surface most prone to a particular contaminant apparently makes it less likely for that contaminant to bind.
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protein lipid interaction on the surface of a Hydrophilic Contact Lens in vitro
Current Eye Research, 1997Co-Authors: Andrew R. Bontempo, Jerry RappAbstract:PURPOSE. To investigate the mechanism of protein-lipid interactions responsible for biofilm formation on the surface of Hydrophilic Contact Lenses in vitro. METHODS. New, never-worn Hydrophilic Contact Lenses were individually incubated in a protein-only, lipid-only, or combination protein-lipid artificial tear solution for 24 hours at 37°C with constant stirring. Deposited lipids were removed with a methanol based extraction procedure, separated using high-performance, thin layer chromatography and quantitatively analyzed densitometrically. Deposited proteins were extracted with 4M urea, separated using gel electrophoresis and quantitatively analyzed densitometrically. RESULTS. The presence of protein in the artificial tear solution has a profound effect on the nature of lipid deposition for each group of Hydrophilic Lens, whereas the presence of lipid has a significant effect on the nature of protein deposition for only a group IV Lens. In addition, the presence of lipid deposits on a group IV Lens decr...
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The efficacy of Hydrophilic Contact Lens cleaning systems in removing protein deposits.
The CLAO journal : official publication of the Contact Lens Association of Ophthalmologists Inc, 1993Co-Authors: Jean Jung, Jerry RappAbstract:New, never-worn, individual Hydrophilic Contact Lenses were incubated in an artificial tear solution (containing lysozyme, albumin, lactoferrin, and glycoprotein) for 24 hours at 37 degrees C with constant stirring. These Lenses were then cleaned following the manufacturer's instructions with one of six commercial cleaning systems: AOSEPT, CONSEPT, Oxysept, ReNu, Opti-Free and thermal disinfection in conjunction with the Allergan Enzymatic Contact Lens Cleaner. The protein remaining on each Lens after cleaning was removed and then quantified by the Bio-Rad Protein Assay. High resolution gel electrophoresis was used to assess the individual protein profile patterns. We found that only one-third to one-half of the protein deposited on a Lens is removed by the above cleaning systems. Of the proteins in the artificial tear solution only lysozyme is removed by cleaning, while lactoferrin, albumin, and glycoprotein tend to remain on the Lens. Since many of the complications experienced by Contact Lens wearers are thought to be related to protein deposits on their Lenses, our results suggest the need for more effective Contact Lens cleaning solutions.
Angelo Macrì - One of the best experts on this subject based on the ideXlab platform.
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forecasting of Hydrophilic Contact Lens tolerance by means of tear ferning test
Graefes Archive for Clinical and Experimental Ophthalmology, 1998Co-Authors: Luigi Ravazzoni, Carlo Ghini, Angelo Macrì, M. RolandoAbstract:· Background: The ability of the tear ferning test to predict future Hydrophilic Contact Lens tolerance was studied. · Methods: The tear ferning test (TFT) was performed on one randomly chosen eye of each of the 116 subjects who came to our Contact Lens clinic for Hydrophilic Contact Lens application. The TFT was performed at the time of enrollment (T0) and then 1 month (T1), 3 months (T2) and 6 months (T3) after Contact Lens fitting. The specificity and the sensitivity of the TFT in identifying future Contact Lens tolerance was then studied. The statistical significance of the differences in behavior through the study period among the subjects with different pre-fitting TFT results was evaluated by means of survival curves. · Results: When only type I ferning was considered as a marker of good tear film conditions, the ability of the TFT to forecast Contact Lens tolerance had 78.95% sensitivity, 78.35% specificity and 78.45% diagnostic precision. The TFT showed sensitivity of 100%, specificity of 86.6% and diagnostic precision of 97.4% when performed after 1 month of Contact Lens wearing (T1). Survival curve analysis showed a statistically significant difference in behavior between the group of subjects with pre-fitting ferning type I and the other three groups (P<0.001). · Conclusion: The TFT appears to have good sensitivity and specific for prediction of Contact Lens tolerance in a clinical setting.
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Forecasting of Hydrophilic Contact Lens tolerance by means of tear ferning test
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 1998Co-Authors: Luigi Ravazzoni, Carlo Ghini, Angelo Macrì, M. RolandoAbstract:The ability of the tear ferning test to predict future Hydrophilic Contact Lens tolerance was studied. The tear ferning test (TFT) was performed on one randomly chosen eye of each of the 116 subjects who came to our Contact Lens clinic for Hydrophilic Contact Lens application. The TFT was performed at the time of enrollment (TO) and then 1 month (T1), 3 months (T2) and 6 months (T3) after Contact Lens fitting. The specificity and the sensitivity of the TFT in identifying future Contact Lens tolerance was then studied. The statistical significance of the differences in behavior through the study period among the subjects with different pre-fitting TFT results was evaluated by means of survival curves. When only type I ferning was considered as a marker of good tear film conditions, the ability of the TFT to forecast Contact Lens tolerance had 78.95% sensitivity, 78.35% specificity and 78.45% diagnostic precision. The TFT showed sensitivity of 100%, specificity of 86.6% and diagnostic precision of 97.4% when performed after 1 month of Contact Lens wearing (T1). Survival curve analysis showed a statistically significant difference in behavior between the group of subjects with pre-fitting ferning type I and the other three groups (P < 0.001). The TFT appears to have good sensitivity and specific for prediction of Contact Lens tolerance in a clinical setting.
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Forecasting of Hydrophilic Contact Lens tolerance by means of tear ferning test
Graefe's Archive for Clinical and Experimental Ophthalmology, 1998Co-Authors: Luigi Ravazzoni, Carlo Ghini, Angelo Macrì, M. RolandoAbstract:· Background: The ability of the tear ferning test to predict future Hydrophilic Contact Lens tolerance was studied. · Methods: The tear ferning test (TFT) was performed on one randomly chosen eye of each of the 116 subjects who came to our Contact Lens clinic for Hydrophilic Contact Lens application. The TFT was performed at the time of enrollment (T0) and then 1 month (T1), 3 months (T2) and 6 months (T3) after Contact Lens fitting. The specificity and the sensitivity of the TFT in identifying future Contact Lens tolerance was then studied. The statistical significance of the differences in behavior through the study period among the subjects with different pre-fitting TFT results was evaluated by means of survival curves. · Results: When only type I ferning was considered as a marker of good tear film conditions, the ability of the TFT to forecast Contact Lens tolerance had 78.95% sensitivity, 78.35% specificity and 78.45% diagnostic precision. The TFT showed sensitivity of 100%, specificity of 86.6% and diagnostic precision of 97.4% when performed after 1 month of Contact Lens wearing (T1). Survival curve analysis showed a statistically significant difference in behavior between the group of subjects with pre-fitting ferning type I and the other three groups ( P
Carlo Ghini - One of the best experts on this subject based on the ideXlab platform.
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forecasting of Hydrophilic Contact Lens tolerance by means of tear ferning test
Graefes Archive for Clinical and Experimental Ophthalmology, 1998Co-Authors: Luigi Ravazzoni, Carlo Ghini, Angelo Macrì, M. RolandoAbstract:· Background: The ability of the tear ferning test to predict future Hydrophilic Contact Lens tolerance was studied. · Methods: The tear ferning test (TFT) was performed on one randomly chosen eye of each of the 116 subjects who came to our Contact Lens clinic for Hydrophilic Contact Lens application. The TFT was performed at the time of enrollment (T0) and then 1 month (T1), 3 months (T2) and 6 months (T3) after Contact Lens fitting. The specificity and the sensitivity of the TFT in identifying future Contact Lens tolerance was then studied. The statistical significance of the differences in behavior through the study period among the subjects with different pre-fitting TFT results was evaluated by means of survival curves. · Results: When only type I ferning was considered as a marker of good tear film conditions, the ability of the TFT to forecast Contact Lens tolerance had 78.95% sensitivity, 78.35% specificity and 78.45% diagnostic precision. The TFT showed sensitivity of 100%, specificity of 86.6% and diagnostic precision of 97.4% when performed after 1 month of Contact Lens wearing (T1). Survival curve analysis showed a statistically significant difference in behavior between the group of subjects with pre-fitting ferning type I and the other three groups (P<0.001). · Conclusion: The TFT appears to have good sensitivity and specific for prediction of Contact Lens tolerance in a clinical setting.
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Forecasting of Hydrophilic Contact Lens tolerance by means of tear ferning test
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 1998Co-Authors: Luigi Ravazzoni, Carlo Ghini, Angelo Macrì, M. RolandoAbstract:The ability of the tear ferning test to predict future Hydrophilic Contact Lens tolerance was studied. The tear ferning test (TFT) was performed on one randomly chosen eye of each of the 116 subjects who came to our Contact Lens clinic for Hydrophilic Contact Lens application. The TFT was performed at the time of enrollment (TO) and then 1 month (T1), 3 months (T2) and 6 months (T3) after Contact Lens fitting. The specificity and the sensitivity of the TFT in identifying future Contact Lens tolerance was then studied. The statistical significance of the differences in behavior through the study period among the subjects with different pre-fitting TFT results was evaluated by means of survival curves. When only type I ferning was considered as a marker of good tear film conditions, the ability of the TFT to forecast Contact Lens tolerance had 78.95% sensitivity, 78.35% specificity and 78.45% diagnostic precision. The TFT showed sensitivity of 100%, specificity of 86.6% and diagnostic precision of 97.4% when performed after 1 month of Contact Lens wearing (T1). Survival curve analysis showed a statistically significant difference in behavior between the group of subjects with pre-fitting ferning type I and the other three groups (P < 0.001). The TFT appears to have good sensitivity and specific for prediction of Contact Lens tolerance in a clinical setting.
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Forecasting of Hydrophilic Contact Lens tolerance by means of tear ferning test
Graefe's Archive for Clinical and Experimental Ophthalmology, 1998Co-Authors: Luigi Ravazzoni, Carlo Ghini, Angelo Macrì, M. RolandoAbstract:· Background: The ability of the tear ferning test to predict future Hydrophilic Contact Lens tolerance was studied. · Methods: The tear ferning test (TFT) was performed on one randomly chosen eye of each of the 116 subjects who came to our Contact Lens clinic for Hydrophilic Contact Lens application. The TFT was performed at the time of enrollment (T0) and then 1 month (T1), 3 months (T2) and 6 months (T3) after Contact Lens fitting. The specificity and the sensitivity of the TFT in identifying future Contact Lens tolerance was then studied. The statistical significance of the differences in behavior through the study period among the subjects with different pre-fitting TFT results was evaluated by means of survival curves. · Results: When only type I ferning was considered as a marker of good tear film conditions, the ability of the TFT to forecast Contact Lens tolerance had 78.95% sensitivity, 78.35% specificity and 78.45% diagnostic precision. The TFT showed sensitivity of 100%, specificity of 86.6% and diagnostic precision of 97.4% when performed after 1 month of Contact Lens wearing (T1). Survival curve analysis showed a statistically significant difference in behavior between the group of subjects with pre-fitting ferning type I and the other three groups ( P
