The Experts below are selected from a list of 285 Experts worldwide ranked by ideXlab platform
William H. Barth - One of the best experts on this subject based on the ideXlab platform.
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the significance of Hyperkeratosis parakeratosis on otherwise normal papanicolaou smears
American Journal of Obstetrics and Gynecology, 2002Co-Authors: Christopher M. Zahn, Kevin L. Hall, Wendy A Askew, William H. BarthAbstract:Abstract Objective: The purpose of this study was to determine whether Hyperkeratosis/parakeratosis found on an otherwise normal Papanicolaou smear was associated with abnormalities after comprehensive evaluation. Study Design: We conducted a retrospective cohort study of 566 women identified with Hyperkeratosis/parakeratosis on Papanicolaou smears from a 7-year period. All women underwent repeat Papanicolaou smear, colposcopy, and biopsy as indicated. Statistical analysis included χ 2 and Fisher exact tests as appropriate. Results: Final abnormal diagnoses included human papillomavirus (HPV) changes (17%), mild dysplasia (5%), and moderate/severe dysplasia (0.4%). We found no carcinomas. Women younger than age 50 years were more likely to have an abnormality (34% vs 19%, relative risk 1.3, 95% CI 1.1-1.4, P P Conclusion: Hyperkeratosis/parakeratosis on an otherwise normal Papanicolaou smear is associated with low-grade changes, particularly among reproductive-age women. (Am J Obstet Gynecol 2002;187:997-1001.)
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The significance of Hyperkeratosis/parakeratosis on otherwise normal Papanicolaou smears
American journal of obstetrics and gynecology, 2002Co-Authors: Christopher M. Zahn, A.wendy Askew, Kevin L. Hall, William H. BarthAbstract:Abstract Objective: The purpose of this study was to determine whether Hyperkeratosis/parakeratosis found on an otherwise normal Papanicolaou smear was associated with abnormalities after comprehensive evaluation. Study Design: We conducted a retrospective cohort study of 566 women identified with Hyperkeratosis/parakeratosis on Papanicolaou smears from a 7-year period. All women underwent repeat Papanicolaou smear, colposcopy, and biopsy as indicated. Statistical analysis included χ 2 and Fisher exact tests as appropriate. Results: Final abnormal diagnoses included human papillomavirus (HPV) changes (17%), mild dysplasia (5%), and moderate/severe dysplasia (0.4%). We found no carcinomas. Women younger than age 50 years were more likely to have an abnormality (34% vs 19%, relative risk 1.3, 95% CI 1.1-1.4, P P Conclusion: Hyperkeratosis/parakeratosis on an otherwise normal Papanicolaou smear is associated with low-grade changes, particularly among reproductive-age women. (Am J Obstet Gynecol 2002;187:997-1001.)
Christopher M. Zahn - One of the best experts on this subject based on the ideXlab platform.
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the significance of Hyperkeratosis parakeratosis on otherwise normal papanicolaou smears
American Journal of Obstetrics and Gynecology, 2002Co-Authors: Christopher M. Zahn, Kevin L. Hall, Wendy A Askew, William H. BarthAbstract:Abstract Objective: The purpose of this study was to determine whether Hyperkeratosis/parakeratosis found on an otherwise normal Papanicolaou smear was associated with abnormalities after comprehensive evaluation. Study Design: We conducted a retrospective cohort study of 566 women identified with Hyperkeratosis/parakeratosis on Papanicolaou smears from a 7-year period. All women underwent repeat Papanicolaou smear, colposcopy, and biopsy as indicated. Statistical analysis included χ 2 and Fisher exact tests as appropriate. Results: Final abnormal diagnoses included human papillomavirus (HPV) changes (17%), mild dysplasia (5%), and moderate/severe dysplasia (0.4%). We found no carcinomas. Women younger than age 50 years were more likely to have an abnormality (34% vs 19%, relative risk 1.3, 95% CI 1.1-1.4, P P Conclusion: Hyperkeratosis/parakeratosis on an otherwise normal Papanicolaou smear is associated with low-grade changes, particularly among reproductive-age women. (Am J Obstet Gynecol 2002;187:997-1001.)
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The significance of Hyperkeratosis/parakeratosis on otherwise normal Papanicolaou smears
American journal of obstetrics and gynecology, 2002Co-Authors: Christopher M. Zahn, A.wendy Askew, Kevin L. Hall, William H. BarthAbstract:Abstract Objective: The purpose of this study was to determine whether Hyperkeratosis/parakeratosis found on an otherwise normal Papanicolaou smear was associated with abnormalities after comprehensive evaluation. Study Design: We conducted a retrospective cohort study of 566 women identified with Hyperkeratosis/parakeratosis on Papanicolaou smears from a 7-year period. All women underwent repeat Papanicolaou smear, colposcopy, and biopsy as indicated. Statistical analysis included χ 2 and Fisher exact tests as appropriate. Results: Final abnormal diagnoses included human papillomavirus (HPV) changes (17%), mild dysplasia (5%), and moderate/severe dysplasia (0.4%). We found no carcinomas. Women younger than age 50 years were more likely to have an abnormality (34% vs 19%, relative risk 1.3, 95% CI 1.1-1.4, P P Conclusion: Hyperkeratosis/parakeratosis on an otherwise normal Papanicolaou smear is associated with low-grade changes, particularly among reproductive-age women. (Am J Obstet Gynecol 2002;187:997-1001.)
Kevin L. Hall - One of the best experts on this subject based on the ideXlab platform.
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the significance of Hyperkeratosis parakeratosis on otherwise normal papanicolaou smears
American Journal of Obstetrics and Gynecology, 2002Co-Authors: Christopher M. Zahn, Kevin L. Hall, Wendy A Askew, William H. BarthAbstract:Abstract Objective: The purpose of this study was to determine whether Hyperkeratosis/parakeratosis found on an otherwise normal Papanicolaou smear was associated with abnormalities after comprehensive evaluation. Study Design: We conducted a retrospective cohort study of 566 women identified with Hyperkeratosis/parakeratosis on Papanicolaou smears from a 7-year period. All women underwent repeat Papanicolaou smear, colposcopy, and biopsy as indicated. Statistical analysis included χ 2 and Fisher exact tests as appropriate. Results: Final abnormal diagnoses included human papillomavirus (HPV) changes (17%), mild dysplasia (5%), and moderate/severe dysplasia (0.4%). We found no carcinomas. Women younger than age 50 years were more likely to have an abnormality (34% vs 19%, relative risk 1.3, 95% CI 1.1-1.4, P P Conclusion: Hyperkeratosis/parakeratosis on an otherwise normal Papanicolaou smear is associated with low-grade changes, particularly among reproductive-age women. (Am J Obstet Gynecol 2002;187:997-1001.)
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The significance of Hyperkeratosis/parakeratosis on otherwise normal Papanicolaou smears
American journal of obstetrics and gynecology, 2002Co-Authors: Christopher M. Zahn, A.wendy Askew, Kevin L. Hall, William H. BarthAbstract:Abstract Objective: The purpose of this study was to determine whether Hyperkeratosis/parakeratosis found on an otherwise normal Papanicolaou smear was associated with abnormalities after comprehensive evaluation. Study Design: We conducted a retrospective cohort study of 566 women identified with Hyperkeratosis/parakeratosis on Papanicolaou smears from a 7-year period. All women underwent repeat Papanicolaou smear, colposcopy, and biopsy as indicated. Statistical analysis included χ 2 and Fisher exact tests as appropriate. Results: Final abnormal diagnoses included human papillomavirus (HPV) changes (17%), mild dysplasia (5%), and moderate/severe dysplasia (0.4%). We found no carcinomas. Women younger than age 50 years were more likely to have an abnormality (34% vs 19%, relative risk 1.3, 95% CI 1.1-1.4, P P Conclusion: Hyperkeratosis/parakeratosis on an otherwise normal Papanicolaou smear is associated with low-grade changes, particularly among reproductive-age women. (Am J Obstet Gynecol 2002;187:997-1001.)
Volker Kromker - One of the best experts on this subject based on the ideXlab platform.
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the association between teat end Hyperkeratosis and teat canal microbial load in lactating dairy cattle
Veterinary Microbiology, 2012Co-Authors: Janhendrik Paduch, Elmar Mohr, Volker KromkerAbstract:Most pathogens that cause bovine mastitis invade the udder lumen through the teat canal. Amino acids and intercellular lipids may support microbial colonisation of the teat canal epithelium by pathogenic microorganisms. The aim of this study was to investigate the association between teat end Hyperkeratosis, which is induced by machine milking, and teat canal microbial load. Contralateral teats, which differed in teat end Hyperkeratosis scores, were identified in a split-udder experiment. The teat canal's microbial load was evaluated using the wet and dry swab technique. Staphylococcus (S.) aureus, Streptococcus (Sc.) uberis, Escherichia (E.) coli and other coliforms were detected by agar plate cultures. The positive detection of E. coli and the log10-transformed E. coli load of a teat canal were significantly associated with the teat end Hyperkeratosis score (P < 0.05). There were significant differences with respect to positive findings for E. coli, as well as the microbial load of E. coli and Sc. uberis, between the less-calloused and the more-calloused teat of a pair. For S. aureus, no significant associations between Hyperkeratosis score and teat canal microbial load were detected. In general, a teat with a highly calloused teat end had an increased teat canal microbial load compared with the contralateral teat, characterised by a lower callosity. The results of the present study indicate that the environmental pathogen load is associated with teat end Hyperkeratosis. Further research is needed to identify factors that may affect teat canal microbial load in lactating dairy cattle.
S J Bale - One of the best experts on this subject based on the ideXlab platform.
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Clinical heterogeneity in epidermolytic Hyperkeratosis.
Archives of Dermatology, 1994Co-Authors: J J Digiovanna, S J BaleAbstract:Background and Design: Epidermolytic Hyperkeratosis (EHK) is a rare autosomal dominant disorder of cornification. While different clinical presentations of EHK have been described, the distinctions have not been clear. We have examined 52 patients with EHK from 21 families in an effort to define and characterize the specific clinical features of this disorder. Results: We found that several features were useful for separating patients with EHK into clinical groups. The most distinctive characteristic was presence vs absence of severe palmoplantar Hyperkeratosis. Twenty-nine patients in six families had this finding and were grouped into ''PS types'' (those with severe palm/sole Hyperkeratosis). The remaining 23 patients (from 15 families) were classified as ''NPS types'' (those without severe palm/ sole Hyperkeratosis). We identified three distinct PS types and three distinct NPS types. The classification was always found to be consistent in all affected family members. In those families in which mutations were defined, keratin 1 mutations were identified in the PS types and keratin 10 mutations in the NPS types. Conclusions: We were able to classify our cohort of 52 patients with EHK from 21 families into distinct types. There was a correlation between presence or absence of severe palm/sole Hyperkeratosis and the specific keratin involved. (Arch Dermatol. 1994;130:1026-1035)
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Mutations in the H1 and 1A Domains in the Keratin 1 Gene in Epidermolytic Hyperkeratosis
Journal of Investigative Dermatology, 1994Co-Authors: Jun-mo Yang, S J Bale, J J Digiovanna, Peter M. Steinert, Constantin C. Chipev, Lyuben N. Marekov, John G. ComptonAbstract:In the autosomal dominant disorder epidermolytic Hyperkeratosis, the structural integrity of the keratin intermediate filaments is altered in the suprabasal layers of the epidermis. We and others have used genetic linkage studies and mutation analysis to establish that single amino acid substitutions in either the keratin 1 or keratin 10 chains can cause epidermolytic Hyperkeratosis. However, a larger database of mutations is required to better understand the relationship between specific mutations in these keratin chains and their effect on keratin filament structure. A larger database will also provide a catalog that may be useful for genetic counseling purposes. In this paper, we report the identification of three new mutations of the keratin 1 chain of epidermolytic Hyperkeratosis probands in highly conserved residues in the H1 or beginning of the 1A rod domain segments. These correspond to regions involved in molecular overlaps between neighboring molecules in keratin filaments. Using an in vitro assay, synthetic peptides bearing these substitutions show diminished capacity to disassemble preformed filaments in vitro in comparison to the wild type peptides. Moreover, analyses of all mutations in epidermolytic Hyperkeratosis known to date demonstrate remarkable clustering in the molecular overlap region. We conclude that non-conservative substitutions in the overlap region are likely to interfere with normal keratin filament structure and function, leading to pathology.
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Epidermolytic Hyperkeratosis: Applied Molecular Genetics
Journal of Investigative Dermatology, 1994Co-Authors: J J Digiovanna, S J BaleAbstract:Epidermolytic Hyperkeratosis is an autosomal dominant ichthyosis characterized by blistering, especially at birth and during childhood, and Hyperkeratosis. Epidermolytic Hyperkeratosis presents striking clinical heterogeneity, particularly between families. Several avenues of research have implicated an abnormality of epidermal differentiation in the pathogenesis of this disease. In a three-generation family with 20 affected individuals, we tested a variety of candidate loci and identified linkage to the type II keratin region on chromosome 12. Further investigation revealed a mutation in the H1 subdomain of the keratin 1 gene as the cause of EHK in this family. Because keratin 10 is the co-expressed partner of keratin 1, it was not surprising when abnormalities in keratin 10 were found in other families with EHK. We have examined 52 patients from 21 families and have identified at least six clinical phenotypes. The most useful distinguishing feature was the presence or absence of severe hyper keratosis of the palms and soles. We and others are continuing to search for and characterize mutations in keratin 1 and 10 in patients with epidermolytic Hyperkeratosis. Correlation of the clinical disease types with the specific mutations should lead to a better understanding of the relationship between keratin structure and function in normal and diseased epidermis.
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Epidermolytic Hyperkeratosis.
Seminars in dermatology, 1993Co-Authors: S J Bale, J G Compton, J J DigiovannaAbstract:Epidermolytic Hyperkeratosis (EHK) is a congenital autosomal dominant ichthyosis. The disorder is characterized by blistering, especially at birth and during childhood, and Hyperkeratosis. EHK presents striking clinical heterogeneity, particularly regarding extent of body surface involvement, quality of scale, presence or absence of erythroderma, and palmar/plantar involvement. Histological examination of hematoxylin-eosin stained sections of EHK skin show a tremendously thickened stratum corneum and vacuolar degeneration of the upper epidermis, leading to the term "epidermolytic Hyperkeratosis". Genetic studies have revealed that defects in either keratin 1 or keratin 10 are responsible for the disorder in several patients, and provide a means for prenatal diagnosis in at-risk pregnancies in these families. An understanding of the relationship of keratin structure to its function in vivo in human epidermis may lead to the development of novel therapies for EHK.
