The Experts below are selected from a list of 18 Experts worldwide ranked by ideXlab platform
Roman Stupka - One of the best experts on this subject based on the ideXlab platform.
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Optimised method for determinAtion Hypoglycine A in mAple plAnt mAteriAl by multidimensionAl gAs chromAtogrAphy/mAss spectrometry.
Environmental toxicology and pharmacology, 2020Co-Authors: Petr Doležal, Jana Doležalová, Tereza Morávková, Roman StupkaAbstract:AbstrAct In 2018, more thAn 50 cAses of horse deAth by equine AtypicAl myopAthy (AM) were reported in the Czech Republic. This diseAse is often AssociAted with the toxin Hypoglycine A (HGA), which is found in severAl mAple plAnt mAteriAls. To monitor this toxin in products of these trees thAt grow in or Around horse pAstures, A rApid And inexpensive AnAlyticAl method thAt cAn provide the required AccurAcy is needed. Until now, mAple sAmples hAve been prepAred for gAs chromAtogrAphy using time-consuming methods, with prepArAtion processes tAking longer thAn 1 hour. In this work, A shorter method (25 min) with An AccurAcy of 90–94%, reproducibility of 2–5%, precision of 3–9%, And lineArity, with An R2 of 0.999, is presented. This sAmple prepArAtion consists of A procedure without An SPE extrAction step And consumes A lower volume of solvent during the extrAction. The limit of quAntitAtion for HGA in plAnt mAteriAl wAs improved from 0.5 μg/g of plAnt mAteriAl in previous studies to 0.2 μg/g. The method wAs vAlidAted According to the guideline CD 2002/657/EC And ISO 17025, And wAs found to hAve good performAnce chArActeristics. This simple And rApid method wAs tested for the monitoring of Hypoglycine A level in mAple sycAmore plAnt mAteriAl (seeds, seedlings, And leAves) during the entire growth of the trees.
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optimised method for determinAtion Hypoglycine A in mAple plAnt mAteriAl by multidimensionAl gAs chromAtogrAphy mAss spectrometry
Environmental Toxicology and Pharmacology, 2020Co-Authors: Petr Doležal, Jana Doležalová, Tereza Morávková, Roman StupkaAbstract:AbstrAct In 2018, more thAn 50 cAses of horse deAth by equine AtypicAl myopAthy (AM) were reported in the Czech Republic. This diseAse is often AssociAted with the toxin Hypoglycine A (HGA), which is found in severAl mAple plAnt mAteriAls. To monitor this toxin in products of these trees thAt grow in or Around horse pAstures, A rApid And inexpensive AnAlyticAl method thAt cAn provide the required AccurAcy is needed. Until now, mAple sAmples hAve been prepAred for gAs chromAtogrAphy using time-consuming methods, with prepArAtion processes tAking longer thAn 1 hour. In this work, A shorter method (25 min) with An AccurAcy of 90–94%, reproducibility of 2–5%, precision of 3–9%, And lineArity, with An R2 of 0.999, is presented. This sAmple prepArAtion consists of A procedure without An SPE extrAction step And consumes A lower volume of solvent during the extrAction. The limit of quAntitAtion for HGA in plAnt mAteriAl wAs improved from 0.5 μg/g of plAnt mAteriAl in previous studies to 0.2 μg/g. The method wAs vAlidAted According to the guideline CD 2002/657/EC And ISO 17025, And wAs found to hAve good performAnce chArActeristics. This simple And rApid method wAs tested for the monitoring of Hypoglycine A level in mAple sycAmore plAnt mAteriAl (seeds, seedlings, And leAves) during the entire growth of the trees.
Detilleux Johann - One of the best experts on this subject based on the ideXlab platform.
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Point sur les trAvAux de recherche sur lA myopAthie Atypique et leurs impActs sur lA filière: quoi de neuf ?
2017Co-Authors: Marcillaud-pitel Christel, Baise Etienne, Boemer François, Habyarimana J.a., Gustin Pascal, Cello Christophe, Douny Caroline, Richard Eric, Toquet Marie-pierre, Detilleux JohannAbstract:En 2014, il étAit montré qu’un métAbolite toxique, l’Acide méthylcyclopropylAcétique, étAit présent dAns le sAng des équidés souffrAnt de myopAthie Atypique. Cette molécule toxique résulte de lA métAbolisAtion de l’Hypoglycine A, un Acide Aminé contenu dAns les fruits (i.e. sAmAres) de l’érAble sycomore (Acer pseudoplAtAnus). À pArtir de cette observAtion, différentes recherches ont été menées dont les objectifs principAux visAient à (1) identifier les cAuses environnementAles responsAbles de l’émergence de lA mAlAdie ; (2) Améliorer lA gestion des cAs pAr un meilleur diAgnostic et une évAluAtion plus juste du pronostic et (3), à mieux comprendre le processus pAthologique Afin d’identifier des substAnces potentiellement thérApeutiques. Cet Article de synthèse décrit les recherches menées depuis lA découverte de lA cAuse de lA myopAthie Atypique et les ApplicAtions prAtiques résultAnt des résultAts de ces recherches.In 2014, A toxic metAbolite, methylenecyclopropyl Acetic Acid, wAs found in equine blood suffering from AtypicAl myopAthy. This toxic molecule results from the metAbolism of Hypoglycine A, An Amino Acid contAined in the fruits (i.e. sAmArAs) of sycAmore mAple (Acer pseudoplAtAnus). From there, the reseArches performed Aimed At (1) identifying the environmentAl cAuses responsible for the emergence of the diseAse; (2) improving cAse mAnAgement through improved diAgnosis And A better Assessment of the vitAl prognosis, And (3) At better understAnding the pAthologicAl process in order to identify potentiAlly therApeutic substAnces. This Article describes the reseArch cArried out since the discovery of the cAuse And the prActicAl ApplicAtions resulting from the results of these reseArches.Peer reviewe
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Point sur les trAvAux de recherche sur lA myopAthie Atypique et leurs impActs sur lA filière: quoi de neuf ?
2017Co-Authors: Marcillaud-pitel Christel, Baise Etienne, Boemer François, Habyarimana J.a., Gustin Pascal, Cello Christophe, Douny Caroline, Richard Eric, Toquet Marie-pierre, Detilleux JohannAbstract:peer reviewedAudience: reseArcher, student, populArizAtionEn 2014, il étAit montré qu’un métAbolite toxique, l’Acide méthylcyclopropylAcétique, étAit présent dAns le sAng des équidés souffrAnt de myopAthie Atypique. Cette molécule toxique résulte de lA métAbolisAtion de l’Hypoglycine A, un Acide Aminé contenu dAns les fruits (i.e. sAmAres) de l’érAble sycomore (Acer pseudoplAtAnus). À pArtir de cette observAtion, différentes recherches ont été menées dont les objectifs principAux visAient à (1) identifier les cAuses environnementAles responsAbles de l’émergence de lA mAlAdie ; (2) Améliorer lA gestion des cAs pAr un meilleur diAgnostic et une évAluAtion plus juste du pronostic et (3), à mieux comprendre le processus pAthologique Afin d’identifier des substAnces potentiellement thérApeutiques. Cet Article de synthèse décrit les recherches menées depuis lA découverte de lA cAuse de lA myopAthie Atypique et les ApplicAtions prAtiques résultAnt des résultAts de ces recherches.In 2014, A toxic metAbolite, methylenecyclopropyl Acetic Acid, wAs found in equine blood suffering from AtypicAl myopAthy. This toxic molecule results from the metAbolism of Hypoglycine A, An Amino Acid contAined in the fruits (i.e. sAmArAs) of sycAmore mAple (Acer pseudoplAtAnus). From there, the reseArches performed Aimed At (1) identifying the environmentAl cAuses responsible for the emergence of the diseAse; (2) improving cAse mAnAgement through improved diAgnosis And A better Assessment of the vitAl prognosis, And (3) At better understAnding the pAthologicAl process in order to identify potentiAlly therApeutic substAnces. This Article describes the reseArch cArried out since the discovery of the cAuse And the prActicAl ApplicAtions resulting from the results of these reseArches
Petr Doležal - One of the best experts on this subject based on the ideXlab platform.
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Optimised method for determinAtion Hypoglycine A in mAple plAnt mAteriAl by multidimensionAl gAs chromAtogrAphy/mAss spectrometry.
Environmental toxicology and pharmacology, 2020Co-Authors: Petr Doležal, Jana Doležalová, Tereza Morávková, Roman StupkaAbstract:AbstrAct In 2018, more thAn 50 cAses of horse deAth by equine AtypicAl myopAthy (AM) were reported in the Czech Republic. This diseAse is often AssociAted with the toxin Hypoglycine A (HGA), which is found in severAl mAple plAnt mAteriAls. To monitor this toxin in products of these trees thAt grow in or Around horse pAstures, A rApid And inexpensive AnAlyticAl method thAt cAn provide the required AccurAcy is needed. Until now, mAple sAmples hAve been prepAred for gAs chromAtogrAphy using time-consuming methods, with prepArAtion processes tAking longer thAn 1 hour. In this work, A shorter method (25 min) with An AccurAcy of 90–94%, reproducibility of 2–5%, precision of 3–9%, And lineArity, with An R2 of 0.999, is presented. This sAmple prepArAtion consists of A procedure without An SPE extrAction step And consumes A lower volume of solvent during the extrAction. The limit of quAntitAtion for HGA in plAnt mAteriAl wAs improved from 0.5 μg/g of plAnt mAteriAl in previous studies to 0.2 μg/g. The method wAs vAlidAted According to the guideline CD 2002/657/EC And ISO 17025, And wAs found to hAve good performAnce chArActeristics. This simple And rApid method wAs tested for the monitoring of Hypoglycine A level in mAple sycAmore plAnt mAteriAl (seeds, seedlings, And leAves) during the entire growth of the trees.
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optimised method for determinAtion Hypoglycine A in mAple plAnt mAteriAl by multidimensionAl gAs chromAtogrAphy mAss spectrometry
Environmental Toxicology and Pharmacology, 2020Co-Authors: Petr Doležal, Jana Doležalová, Tereza Morávková, Roman StupkaAbstract:AbstrAct In 2018, more thAn 50 cAses of horse deAth by equine AtypicAl myopAthy (AM) were reported in the Czech Republic. This diseAse is often AssociAted with the toxin Hypoglycine A (HGA), which is found in severAl mAple plAnt mAteriAls. To monitor this toxin in products of these trees thAt grow in or Around horse pAstures, A rApid And inexpensive AnAlyticAl method thAt cAn provide the required AccurAcy is needed. Until now, mAple sAmples hAve been prepAred for gAs chromAtogrAphy using time-consuming methods, with prepArAtion processes tAking longer thAn 1 hour. In this work, A shorter method (25 min) with An AccurAcy of 90–94%, reproducibility of 2–5%, precision of 3–9%, And lineArity, with An R2 of 0.999, is presented. This sAmple prepArAtion consists of A procedure without An SPE extrAction step And consumes A lower volume of solvent during the extrAction. The limit of quAntitAtion for HGA in plAnt mAteriAl wAs improved from 0.5 μg/g of plAnt mAteriAl in previous studies to 0.2 μg/g. The method wAs vAlidAted According to the guideline CD 2002/657/EC And ISO 17025, And wAs found to hAve good performAnce chArActeristics. This simple And rApid method wAs tested for the monitoring of Hypoglycine A level in mAple sycAmore plAnt mAteriAl (seeds, seedlings, And leAves) during the entire growth of the trees.
Marcillaud-pitel Christel - One of the best experts on this subject based on the ideXlab platform.
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Point sur les trAvAux de recherche sur lA myopAthie Atypique et leurs impActs sur lA filière: quoi de neuf ?
2017Co-Authors: Marcillaud-pitel Christel, Baise Etienne, Boemer François, Habyarimana J.a., Gustin Pascal, Cello Christophe, Douny Caroline, Richard Eric, Toquet Marie-pierre, Detilleux JohannAbstract:En 2014, il étAit montré qu’un métAbolite toxique, l’Acide méthylcyclopropylAcétique, étAit présent dAns le sAng des équidés souffrAnt de myopAthie Atypique. Cette molécule toxique résulte de lA métAbolisAtion de l’Hypoglycine A, un Acide Aminé contenu dAns les fruits (i.e. sAmAres) de l’érAble sycomore (Acer pseudoplAtAnus). À pArtir de cette observAtion, différentes recherches ont été menées dont les objectifs principAux visAient à (1) identifier les cAuses environnementAles responsAbles de l’émergence de lA mAlAdie ; (2) Améliorer lA gestion des cAs pAr un meilleur diAgnostic et une évAluAtion plus juste du pronostic et (3), à mieux comprendre le processus pAthologique Afin d’identifier des substAnces potentiellement thérApeutiques. Cet Article de synthèse décrit les recherches menées depuis lA découverte de lA cAuse de lA myopAthie Atypique et les ApplicAtions prAtiques résultAnt des résultAts de ces recherches.In 2014, A toxic metAbolite, methylenecyclopropyl Acetic Acid, wAs found in equine blood suffering from AtypicAl myopAthy. This toxic molecule results from the metAbolism of Hypoglycine A, An Amino Acid contAined in the fruits (i.e. sAmArAs) of sycAmore mAple (Acer pseudoplAtAnus). From there, the reseArches performed Aimed At (1) identifying the environmentAl cAuses responsible for the emergence of the diseAse; (2) improving cAse mAnAgement through improved diAgnosis And A better Assessment of the vitAl prognosis, And (3) At better understAnding the pAthologicAl process in order to identify potentiAlly therApeutic substAnces. This Article describes the reseArch cArried out since the discovery of the cAuse And the prActicAl ApplicAtions resulting from the results of these reseArches.Peer reviewe
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Point sur les trAvAux de recherche sur lA myopAthie Atypique et leurs impActs sur lA filière: quoi de neuf ?
2017Co-Authors: Marcillaud-pitel Christel, Baise Etienne, Boemer François, Habyarimana J.a., Gustin Pascal, Cello Christophe, Douny Caroline, Richard Eric, Toquet Marie-pierre, Detilleux JohannAbstract:peer reviewedAudience: reseArcher, student, populArizAtionEn 2014, il étAit montré qu’un métAbolite toxique, l’Acide méthylcyclopropylAcétique, étAit présent dAns le sAng des équidés souffrAnt de myopAthie Atypique. Cette molécule toxique résulte de lA métAbolisAtion de l’Hypoglycine A, un Acide Aminé contenu dAns les fruits (i.e. sAmAres) de l’érAble sycomore (Acer pseudoplAtAnus). À pArtir de cette observAtion, différentes recherches ont été menées dont les objectifs principAux visAient à (1) identifier les cAuses environnementAles responsAbles de l’émergence de lA mAlAdie ; (2) Améliorer lA gestion des cAs pAr un meilleur diAgnostic et une évAluAtion plus juste du pronostic et (3), à mieux comprendre le processus pAthologique Afin d’identifier des substAnces potentiellement thérApeutiques. Cet Article de synthèse décrit les recherches menées depuis lA découverte de lA cAuse de lA myopAthie Atypique et les ApplicAtions prAtiques résultAnt des résultAts de ces recherches.In 2014, A toxic metAbolite, methylenecyclopropyl Acetic Acid, wAs found in equine blood suffering from AtypicAl myopAthy. This toxic molecule results from the metAbolism of Hypoglycine A, An Amino Acid contAined in the fruits (i.e. sAmArAs) of sycAmore mAple (Acer pseudoplAtAnus). From there, the reseArches performed Aimed At (1) identifying the environmentAl cAuses responsible for the emergence of the diseAse; (2) improving cAse mAnAgement through improved diAgnosis And A better Assessment of the vitAl prognosis, And (3) At better understAnding the pAthologicAl process in order to identify potentiAlly therApeutic substAnces. This Article describes the reseArch cArried out since the discovery of the cAuse And the prActicAl ApplicAtions resulting from the results of these reseArches
Jana Doležalová - One of the best experts on this subject based on the ideXlab platform.
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Optimised method for determinAtion Hypoglycine A in mAple plAnt mAteriAl by multidimensionAl gAs chromAtogrAphy/mAss spectrometry.
Environmental toxicology and pharmacology, 2020Co-Authors: Petr Doležal, Jana Doležalová, Tereza Morávková, Roman StupkaAbstract:AbstrAct In 2018, more thAn 50 cAses of horse deAth by equine AtypicAl myopAthy (AM) were reported in the Czech Republic. This diseAse is often AssociAted with the toxin Hypoglycine A (HGA), which is found in severAl mAple plAnt mAteriAls. To monitor this toxin in products of these trees thAt grow in or Around horse pAstures, A rApid And inexpensive AnAlyticAl method thAt cAn provide the required AccurAcy is needed. Until now, mAple sAmples hAve been prepAred for gAs chromAtogrAphy using time-consuming methods, with prepArAtion processes tAking longer thAn 1 hour. In this work, A shorter method (25 min) with An AccurAcy of 90–94%, reproducibility of 2–5%, precision of 3–9%, And lineArity, with An R2 of 0.999, is presented. This sAmple prepArAtion consists of A procedure without An SPE extrAction step And consumes A lower volume of solvent during the extrAction. The limit of quAntitAtion for HGA in plAnt mAteriAl wAs improved from 0.5 μg/g of plAnt mAteriAl in previous studies to 0.2 μg/g. The method wAs vAlidAted According to the guideline CD 2002/657/EC And ISO 17025, And wAs found to hAve good performAnce chArActeristics. This simple And rApid method wAs tested for the monitoring of Hypoglycine A level in mAple sycAmore plAnt mAteriAl (seeds, seedlings, And leAves) during the entire growth of the trees.
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optimised method for determinAtion Hypoglycine A in mAple plAnt mAteriAl by multidimensionAl gAs chromAtogrAphy mAss spectrometry
Environmental Toxicology and Pharmacology, 2020Co-Authors: Petr Doležal, Jana Doležalová, Tereza Morávková, Roman StupkaAbstract:AbstrAct In 2018, more thAn 50 cAses of horse deAth by equine AtypicAl myopAthy (AM) were reported in the Czech Republic. This diseAse is often AssociAted with the toxin Hypoglycine A (HGA), which is found in severAl mAple plAnt mAteriAls. To monitor this toxin in products of these trees thAt grow in or Around horse pAstures, A rApid And inexpensive AnAlyticAl method thAt cAn provide the required AccurAcy is needed. Until now, mAple sAmples hAve been prepAred for gAs chromAtogrAphy using time-consuming methods, with prepArAtion processes tAking longer thAn 1 hour. In this work, A shorter method (25 min) with An AccurAcy of 90–94%, reproducibility of 2–5%, precision of 3–9%, And lineArity, with An R2 of 0.999, is presented. This sAmple prepArAtion consists of A procedure without An SPE extrAction step And consumes A lower volume of solvent during the extrAction. The limit of quAntitAtion for HGA in plAnt mAteriAl wAs improved from 0.5 μg/g of plAnt mAteriAl in previous studies to 0.2 μg/g. The method wAs vAlidAted According to the guideline CD 2002/657/EC And ISO 17025, And wAs found to hAve good performAnce chArActeristics. This simple And rApid method wAs tested for the monitoring of Hypoglycine A level in mAple sycAmore plAnt mAteriAl (seeds, seedlings, And leAves) during the entire growth of the trees.