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Marc Stadler - One of the best experts on this subject based on the ideXlab platform.
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Discovery of a new species of the Hypoxylon rubiginosum complex from Iran and antagonistic activities of Hypoxylon spp. against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in dual culture.
MycoKeys, 2020Co-Authors: Mohammad Javad Pourmoghaddam, Frank Surup, Christopher Lambert, Seyed Akbar Khodaparast, Irmgard Krisai-greilhuber, Hermann Voglmayr, Marc StadlerAbstract:During a survey of xylarialean fungi in Northern Iran, several specimens that showed affinities to the Hypoxylon rubiginosum complex were collected and cultured. A comparison of their morphological characters, combined with a chemotaxonomic study based on high performance liquid chromatography, coupled with diode array detection and mass spectrometry (HPLC-DAD/MS) and a multi-locus phylogeny based on ITS, LSU, rbp2 and tub2 DNA sequences, revealed a new species here described as Hypoxylon guilanense. In addition, Hypoxylon rubiginosum sensu stricto was also encountered. Concurrently, an endophytic isolate of the latter species showed strong antagonistic activities against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in a dual culture assay in our laboratory. Therefore, we decided to test the new Iranian fungi for antagonistic activities against the pathogen, along with several cultures of other Hypoxylon species that are related to H. rubiginosum. Our results suggest that the antagonistic effects of Hypoxylon spp. against Hym. fraxineus are widespread and that they are due to the production of antifungal phomopsidin derivatives in the presence of the pathogen.
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hypomontagnella hypoxylaceae a new genus segregated from Hypoxylon by a polyphasic taxonomic approach
Mycological Progress, 2019Co-Authors: Christopher Lambert, Adriana I Hladki, Lucile Wendt, Marc StadlerAbstract:During a survey of xylarialean Sordariomycetes in North-western Argentina, several collections representing a new species morphologically resembling the pantropical Hypoxylon monticulosum was encountered. They were studied by a combination of morphological and chemotaxonomic studies along with a multi-locus phylogeny of Hypoxylon and related genera of the Hypoxylaceae based on a combined ITS, LSU, TUB2, and RBP2 dataset. The phylogenetic tree revealed that DNA sequences of the new fungus clustered together with Hypoxylon monticulosum and Hypoxylon submonticulosum in a separate clade. In addition, all representatives of this clade produced the antifungal polyketides of the sporothriolide type in standardized submerged cultures. Furthermore, a comparison of the morphology of this group of species formerly placed in Hypoxylon, including H. austrobahiense and H. rubigineoareolatum, also revealed certain morphological similarities. They are therefore accommodated in the new genus Hypomontagnella, with Hypomontagnella monticulosa as type species, and the new Argentine fungus is described as Hypomontagnella barbarensis.
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volatiles from the hypoxylaceous fungi Hypoxylon griseobrunneum and Hypoxylon macrocarpum
Beilstein Journal of Organic Chemistry, 2018Co-Authors: Jan Rinkel, Marc Stadler, Alexander Babczyk, Tao Wang, Jeroen S DickschatAbstract:The volatiles emitted by the ascomycetes Hypoxylon griseobrunneum and Hypoxylon macrocarpum (Hypoxylaceae, Xylariales) were collected by use of a closed-loop stripping apparatus (CLSA) and analysed by GC–MS. The main compound class of both species were polysubstituted benzene derivatives. Their structures could only be unambiguously determined by comparison to all isomers with different substitution patterns. The substitution pattern of the main compound from H. griseobrunneum, the new natural product 2,4,5-trimethylanisole, was explainable by a polyketide biosynthesis mechanism that was supported by a feeding experiment with (methyl-2H3)methionine.
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new species and reports of Hypoxylon from argentina recognized by a polyphasic approach
Mycological Progress, 2016Co-Authors: Eric Kuhnert, Christopher Lambert, Adriana I Hladki, Andrea I Romero, Marc StadlerAbstract:A preliminary account of Hypoxylon species (Xylariaceae) from the hitherto widely unexplored “Yungas” mountain forests of Northwest Argentina is presented. Two new species are described based on extensive morphological, molecular (ITS region of rDNA, partial β-tubulin gene) and chemotaxonomic data. Hypoxylon spegazzinianum is close to H. erythrostroma, but differs by larger ascospores and a virgariella-like asexual morph. Hypoxylon calileguense resembles H. subgilvum when growing on wood, but can be distinguished by larger ascospores and a fawn to brick stromatal surface colour. Stromata found on bark have affinities to H. pelliculosum, but differ in their stromatal surface colour and conspicuous amyloid apical apparatus. In addition, nine taxa of Hypoxylon are reported for Argentina for the first time, and some details on their asexual state and stromatal secondary metabolites are reported. An updated dichotomous key for Hypoxylon species from Argentina is provided.
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botryane noreudesmane and abietane terpenoids from the ascomycete Hypoxylon rickii
Phytochemistry, 2015Co-Authors: Eric Kuhnert, Frank Surup, Vincent Wiebach, Steffen Bernecker, Marc StadlerAbstract:In the course of our screening for new bioactive natural products, a culture of Hypoxylon rickii, a xylariaceous ascomycete collected from the Caribbean island Martinique, was identified as extraordinary prolific producer of secondary metabolites. Ten metabolites of terpenoid origin were isolated from submerged cultures of this species by preparative HPLC. Their structures were elucidated using spectral techniques including 2D NMR and HRESIMS. Three of the compounds were elucidated as new botryanes (1–3) along with three known ones, i.e. (3aS)-3a,5,5,8-tetramethyl-3,3a,4,5-tetrahydro-1H-cyclopenta[de]isochromen-1-one (4), (3aS,8R)-3a,5,5,8-tetramethyl-3,3a,4,5,7,8-hexahydro-1H-cyclopenta[de]isochromen-1-one (5) and botryenanol (6). Further three new sesquiterpenoids featured a 14-noreudesmane-type skeleton and were named hypoxylan A–C (7–9); the diterpenoid rickitin A (10) contains an abietane-type backbone. Compounds 1, 2, 3, 7, and 10 showed cytotoxic effects against murine cells.
Eric Kuhnert - One of the best experts on this subject based on the ideXlab platform.
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observations on texas Hypoxylons including two new Hypoxylon species and widespread environmental isolates of the h croceum complex identified by a polyphasic approach
Mycologia, 2019Co-Authors: Kevin Becker, Gerald F. Bills, Christopher Lambert, Eric KuhnertAbstract:Two new species and a new combination of Hypoxylon from Texas were identified and described based on morphological, multigene phylogenetic (ITS [nuc rDNA internal transcribed spacer region ITS1-5.8...
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new species and reports of Hypoxylon from argentina recognized by a polyphasic approach
Mycological Progress, 2016Co-Authors: Eric Kuhnert, Christopher Lambert, Adriana I Hladki, Andrea I Romero, Marc StadlerAbstract:A preliminary account of Hypoxylon species (Xylariaceae) from the hitherto widely unexplored “Yungas” mountain forests of Northwest Argentina is presented. Two new species are described based on extensive morphological, molecular (ITS region of rDNA, partial β-tubulin gene) and chemotaxonomic data. Hypoxylon spegazzinianum is close to H. erythrostroma, but differs by larger ascospores and a virgariella-like asexual morph. Hypoxylon calileguense resembles H. subgilvum when growing on wood, but can be distinguished by larger ascospores and a fawn to brick stromatal surface colour. Stromata found on bark have affinities to H. pelliculosum, but differ in their stromatal surface colour and conspicuous amyloid apical apparatus. In addition, nine taxa of Hypoxylon are reported for Argentina for the first time, and some details on their asexual state and stromatal secondary metabolites are reported. An updated dichotomous key for Hypoxylon species from Argentina is provided.
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botryane noreudesmane and abietane terpenoids from the ascomycete Hypoxylon rickii
Phytochemistry, 2015Co-Authors: Eric Kuhnert, Frank Surup, Vincent Wiebach, Steffen Bernecker, Marc StadlerAbstract:In the course of our screening for new bioactive natural products, a culture of Hypoxylon rickii, a xylariaceous ascomycete collected from the Caribbean island Martinique, was identified as extraordinary prolific producer of secondary metabolites. Ten metabolites of terpenoid origin were isolated from submerged cultures of this species by preparative HPLC. Their structures were elucidated using spectral techniques including 2D NMR and HRESIMS. Three of the compounds were elucidated as new botryanes (1–3) along with three known ones, i.e. (3aS)-3a,5,5,8-tetramethyl-3,3a,4,5-tetrahydro-1H-cyclopenta[de]isochromen-1-one (4), (3aS,8R)-3a,5,5,8-tetramethyl-3,3a,4,5,7,8-hexahydro-1H-cyclopenta[de]isochromen-1-one (5) and botryenanol (6). Further three new sesquiterpenoids featured a 14-noreudesmane-type skeleton and were named hypoxylan A–C (7–9); the diterpenoid rickitin A (10) contains an abietane-type backbone. Compounds 1, 2, 3, 7, and 10 showed cytotoxic effects against murine cells.
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Lenormandins A—G, new azaphilones from Hypoxylon lenormandii and Hypoxylon jaklitschii sp. nov., recognised by chemotaxonomic data
Fungal Diversity, 2015Co-Authors: Eric Kuhnert, Frank Surup, Christopher Lambert, Adriana I Hladki, Andrea I Romero, Kevin D Hyde, Marc StadlerAbstract:Seven new azaphilone pigments named lenormandins A–G were isolated from stromata (fruiting bodies) of the xylariaceous fungus, Hypoxylon lenormandii using preparative High Performance Liquid Chromatography (HPLC) and their structures were elucidated by NMR spectroscopy, high resolution mass spectrometry and other spectral methods. Their occurrence in herbarium specimens (including various type materials collected in the 19th and early 20th century) and several fresh collections was studied by analytical HPLC with diode array and mass spectrometric detection (HPLC-DAD/MS), revealing that this group of pigments is specific for Hypoxylon lenormandii from various geographic regions and otherwise only occurs in closely related taxa, i.e. H. sublenormandii and an undescribed fungus from Sri Lanka, for which we propose the name H. jaklitschii . The status of the new species was established by using molecular phylogenetic data based on ITS and partial beta-tubulin sequences, and by detailed morphological studies.
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lenormandins a g new azaphilones from Hypoxylon lenormandii and Hypoxylon jaklitschii sp nov recognised by chemotaxonomic data
Fungal Diversity, 2015Co-Authors: Eric Kuhnert, Frank Surup, Christopher Lambert, Adriana I Hladki, Andrea I Romero, Kevin D Hyde, Marc StadlerAbstract:Seven new azaphilone pigments named lenormandins A–G were isolated from stromata (fruiting bodies) of the xylariaceous fungus, Hypoxylon lenormandii using preparative High Performance Liquid Chromatography (HPLC) and their structures were elucidated by NMR spectroscopy, high resolution mass spectrometry and other spectral methods. Their occurrence in herbarium specimens (including various type materials collected in the 19th and early 20th century) and several fresh collections was studied by analytical HPLC with diode array and mass spectrometric detection (HPLC-DAD/MS), revealing that this group of pigments is specific for Hypoxylon lenormandii from various geographic regions and otherwise only occurs in closely related taxa, i.e. H. sublenormandii and an undescribed fungus from Sri Lanka, for which we propose the name H. jaklitschii. The status of the new species was established by using molecular phylogenetic data based on ITS and partial beta-tubulin sequences, and by detailed morphological studies.
Yoshinori Asakawa - One of the best experts on this subject based on the ideXlab platform.
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changes in secondary metabolism during stromatal ontogeny of Hypoxylon fragiforme
Fungal Biology, 2006Co-Authors: Marc Stadler, Dang Ngoc Quang, Toshihiro Hashimoto, Ayumi Tomita, Yoshinori AsakawaAbstract:Stromata of Hypoxylon fragiforme were studied during the vegetation period by hplc profiling, revealing changes in the composition during stromatal development. Cytochalasin H and two new cytochalasins named fragiformins A-B were identified as major constituents of the young, maturing stromata, whereas mature, ascogenous material yielded large amounts of mitorubrin-type azaphilones. The above compounds, further cytochalasins from Xylariaceae and other fungi, and additional azaphilones of the mitorubrin type were assayed for their nematicidal effects against Caenorhabditis elegans and their antimicrobial activities against Bacillus subtilis, Yarrowia lipolytica, and various filamentous fungi. The results confirmed data in the literature on broad-spectrum non-selective activities of azaphilones and cytochalasins in biological systems. Most interestingly, laboratory cultures of the above Hypoxylon spp. mainly produced dihydroisocoumarin derivatives and were found devoid of mitorubrins and cytochalasins. These rather drastic changes in the secondary metabolism of H. fragiforme and the above biological activities are discussed in relation to the possible biological functions of secondary metabolites (extrolites) in the Hypoxyloideae.
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antimicrobial azaphilones from the fungus Hypoxylon multiforme
Planta Medica, 2005Co-Authors: Dang Ngoc Quang, Marc Stadler, Toshihiro Hashimoto, Niko S Radulovic, Yoshinori AsakawaAbstract:Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima, Japan; Bayer Health CareAG, PH-R and D-R-EU-ET1, Wuppertal, Germany; Department of Chemistry, Faculty of Sciences andMathematics, University of Nis, Nis, Yugoslavia; Faculty of Chemistry, Hanoi University of Education,Hanoi, Viet Nam; Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho,Tokushima 770-8514, Japan; Bayer Health Care AG, PH-R and D-R-EU-ET1, P.O. Box 101709, 42096Wuppertal, Germany Abstract: Four new azaphilones named multiformins A - D together with a known compound 4:5:4′:5′-tetrahydroxy-1:1′-binaphthyl were isolated from the methanolic stromatal extract of the xylaria-ceousascomycete Hypoxylon multiforme. Their absolute structures were characterised by 2D-NMR, UV, IR, massand CD spectroscopy. Multiformins A - D showed strong and apparently non-selective antimicrobialactivity. © Georg Thieme Verlag KG Stuttgart. Author Keywords: Antimicrobial; Azaphilone; Fungus; Hypoxylon multiforme; Multiformin Index Keywords: 4:5:4':5' tetrahydroxy 1:1' binaphthyl; antiinfective agent; azaphilone; cholesterol estertransfer protein; methanol; multiformin a; multiformin b; multiformin c; multiformin d; unclassified drug;analytic method; antimicrobial activity; article; Ascomycetes; drug isolation; enzyme inhibition; fungusgrowth; Hypoxylon multiforme; proton nuclear magnetic resonance; structure analysis; Anti-InfectiveAgents; Benzopyrans; Gram-Negative Bacteria; Mitosporic Fungi; Nuclear Magnetic Resonance,Biomolecular; Pigments, Biological; Staphylococcus aureus; Xylariales; AnnuloHypoxylon multiforme;Ascomycota; Fungi; Xylaria Year: 2005 Source title: Planta Medica Volume: 71 Issue: 11 Page : 1058-1062 Cited by: 10 Link: Scorpus Link Chemicals/CAS: methanol, 67-56-1; Anti-Infective Agents; azaphilone; Benzopyrans; Pigments, Biological Correspondence Address: Asakawa, Y.; Faculty of Pharmaceutical Sciences, Tokushima Bunri University,Yamashiro-cho, Tokushima 770-8514, Japan; email: asakawa@ph.bunri-u.ac.jp ISSN: 320943 CODEN: PLMEA DOI: 10.1055/s-2005-873129 PubMed ID: 16320209 Language of Original Document: English
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cohaerins a and b azaphilones from the fungus Hypoxylon cohaerens and comparison of hplc based metabolite profiles in Hypoxylon sect annulata
Phytochemistry, 2005Co-Authors: Dang Ngoc Quang, Marc Stadler, Toshihiro Hashimoto, Yoko Nomura, Hartmund Wollweber, Veronika Hellwig, Jacques Fournier, Yoshinori AsakawaAbstract:Azaphilones, named cohaerins A and B were isolated from stromata of the xylariaceous ascomycete Hypoxylon cohaerens. Their absolute structures were determined by spectroscopic methods (2D NMR, MS, IR, UV CD), and subsequently confirmed by acetylation. Stromatal metabolite profiles of several taxa of Hypoxylon sect. Annulata were also generated using analytical HPLC with diode array and MS detection. The cohaerins were neither found in other Hypoxylon spp., nor in other Xylariaceae. However, they were present even in holotype material of H. cohaerens, collected over 200 years ago. The binaphthalene BNT was also omnipresent in sect Annulata, and its derivatives, the benzo[j]fluoranthenes daldinone A and truncatone, as well as presumably related compounds. These fungi were found devoid of other types of azaphilone pigments of the Xylariaceae, such as mitorubrins and daldinins, the latter of which are widespread in certain groups of Hypoxylon sect. Hypoxylon. Hence, chemotaxonomic data largely support the current generic concept. The original source of truncatone was identified as Hypoxylon annulatum.
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cyclic azaphilones daldinins e and f from the ascomycete fungus Hypoxylon fuscum xylariaceae
Phytochemistry, 2004Co-Authors: Dang Ngoc Quang, Marc Stadler, Toshihiro Hashimoto, Masami Tanaka, Yoshinori AsakawaAbstract:Abstract Phytochemical examination of the methanolic extract of fruit bodies of the Xylariaceous ascomycete fungus Hypoxylon fuscum led to the isolation of two azaphilone derivatives named daldinins E and F together with two known compounds daldinin C and 4,5,4′,5′-tetrahydroxy-1:1′-binaphthyl using a combination of reversed phase HPLC and high performance gel permeation chromatography. Their structures were determined by 2D NMR, MS, IR, UV, and CD spectroscopy. Their antioxidative activities were also estimated by an indication of a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging effect. The chemosystematics of Hypoxylon is discussed.
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occurrence of a high concentration of spider pheromones in the ascomycete fungus Hypoxylon truncatum
Journal of Natural Products, 2003Co-Authors: Dang Ngoc Quang, Toshihiro Hashimoto, Masao Toyota, Yoshinori AsakawaAbstract:A large amount of sex pheromones of the European spider Linyphia triangularis, 3R-hydroxybutyric acid (1), its dimer 3R-(3R-hydroxybutyryloxy)butyric acid (2), and trimer 3R-[3R-(3R-hydroxybutyryloxy)butyryloxy]butyric acid (3) were isolated from the EtOAc extract of the Japanese inedible mushroom Hypoxylon truncatum.
Christopher Lambert - One of the best experts on this subject based on the ideXlab platform.
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Discovery of a new species of the Hypoxylon rubiginosum complex from Iran and antagonistic activities of Hypoxylon spp. against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in dual culture.
MycoKeys, 2020Co-Authors: Mohammad Javad Pourmoghaddam, Frank Surup, Christopher Lambert, Seyed Akbar Khodaparast, Irmgard Krisai-greilhuber, Hermann Voglmayr, Marc StadlerAbstract:During a survey of xylarialean fungi in Northern Iran, several specimens that showed affinities to the Hypoxylon rubiginosum complex were collected and cultured. A comparison of their morphological characters, combined with a chemotaxonomic study based on high performance liquid chromatography, coupled with diode array detection and mass spectrometry (HPLC-DAD/MS) and a multi-locus phylogeny based on ITS, LSU, rbp2 and tub2 DNA sequences, revealed a new species here described as Hypoxylon guilanense. In addition, Hypoxylon rubiginosum sensu stricto was also encountered. Concurrently, an endophytic isolate of the latter species showed strong antagonistic activities against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in a dual culture assay in our laboratory. Therefore, we decided to test the new Iranian fungi for antagonistic activities against the pathogen, along with several cultures of other Hypoxylon species that are related to H. rubiginosum. Our results suggest that the antagonistic effects of Hypoxylon spp. against Hym. fraxineus are widespread and that they are due to the production of antifungal phomopsidin derivatives in the presence of the pathogen.
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observations on texas Hypoxylons including two new Hypoxylon species and widespread environmental isolates of the h croceum complex identified by a polyphasic approach
Mycologia, 2019Co-Authors: Kevin Becker, Gerald F. Bills, Christopher Lambert, Eric KuhnertAbstract:Two new species and a new combination of Hypoxylon from Texas were identified and described based on morphological, multigene phylogenetic (ITS [nuc rDNA internal transcribed spacer region ITS1-5.8...
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hypomontagnella hypoxylaceae a new genus segregated from Hypoxylon by a polyphasic taxonomic approach
Mycological Progress, 2019Co-Authors: Christopher Lambert, Adriana I Hladki, Lucile Wendt, Marc StadlerAbstract:During a survey of xylarialean Sordariomycetes in North-western Argentina, several collections representing a new species morphologically resembling the pantropical Hypoxylon monticulosum was encountered. They were studied by a combination of morphological and chemotaxonomic studies along with a multi-locus phylogeny of Hypoxylon and related genera of the Hypoxylaceae based on a combined ITS, LSU, TUB2, and RBP2 dataset. The phylogenetic tree revealed that DNA sequences of the new fungus clustered together with Hypoxylon monticulosum and Hypoxylon submonticulosum in a separate clade. In addition, all representatives of this clade produced the antifungal polyketides of the sporothriolide type in standardized submerged cultures. Furthermore, a comparison of the morphology of this group of species formerly placed in Hypoxylon, including H. austrobahiense and H. rubigineoareolatum, also revealed certain morphological similarities. They are therefore accommodated in the new genus Hypomontagnella, with Hypomontagnella monticulosa as type species, and the new Argentine fungus is described as Hypomontagnella barbarensis.
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new species and reports of Hypoxylon from argentina recognized by a polyphasic approach
Mycological Progress, 2016Co-Authors: Eric Kuhnert, Christopher Lambert, Adriana I Hladki, Andrea I Romero, Marc StadlerAbstract:A preliminary account of Hypoxylon species (Xylariaceae) from the hitherto widely unexplored “Yungas” mountain forests of Northwest Argentina is presented. Two new species are described based on extensive morphological, molecular (ITS region of rDNA, partial β-tubulin gene) and chemotaxonomic data. Hypoxylon spegazzinianum is close to H. erythrostroma, but differs by larger ascospores and a virgariella-like asexual morph. Hypoxylon calileguense resembles H. subgilvum when growing on wood, but can be distinguished by larger ascospores and a fawn to brick stromatal surface colour. Stromata found on bark have affinities to H. pelliculosum, but differ in their stromatal surface colour and conspicuous amyloid apical apparatus. In addition, nine taxa of Hypoxylon are reported for Argentina for the first time, and some details on their asexual state and stromatal secondary metabolites are reported. An updated dichotomous key for Hypoxylon species from Argentina is provided.
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Lenormandins A—G, new azaphilones from Hypoxylon lenormandii and Hypoxylon jaklitschii sp. nov., recognised by chemotaxonomic data
Fungal Diversity, 2015Co-Authors: Eric Kuhnert, Frank Surup, Christopher Lambert, Adriana I Hladki, Andrea I Romero, Kevin D Hyde, Marc StadlerAbstract:Seven new azaphilone pigments named lenormandins A–G were isolated from stromata (fruiting bodies) of the xylariaceous fungus, Hypoxylon lenormandii using preparative High Performance Liquid Chromatography (HPLC) and their structures were elucidated by NMR spectroscopy, high resolution mass spectrometry and other spectral methods. Their occurrence in herbarium specimens (including various type materials collected in the 19th and early 20th century) and several fresh collections was studied by analytical HPLC with diode array and mass spectrometric detection (HPLC-DAD/MS), revealing that this group of pigments is specific for Hypoxylon lenormandii from various geographic regions and otherwise only occurs in closely related taxa, i.e. H. sublenormandii and an undescribed fungus from Sri Lanka, for which we propose the name H. jaklitschii . The status of the new species was established by using molecular phylogenetic data based on ITS and partial beta-tubulin sequences, and by detailed morphological studies.
Andrea I Romero - One of the best experts on this subject based on the ideXlab platform.
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new species and reports of Hypoxylon from argentina recognized by a polyphasic approach
Mycological Progress, 2016Co-Authors: Eric Kuhnert, Christopher Lambert, Adriana I Hladki, Andrea I Romero, Marc StadlerAbstract:A preliminary account of Hypoxylon species (Xylariaceae) from the hitherto widely unexplored “Yungas” mountain forests of Northwest Argentina is presented. Two new species are described based on extensive morphological, molecular (ITS region of rDNA, partial β-tubulin gene) and chemotaxonomic data. Hypoxylon spegazzinianum is close to H. erythrostroma, but differs by larger ascospores and a virgariella-like asexual morph. Hypoxylon calileguense resembles H. subgilvum when growing on wood, but can be distinguished by larger ascospores and a fawn to brick stromatal surface colour. Stromata found on bark have affinities to H. pelliculosum, but differ in their stromatal surface colour and conspicuous amyloid apical apparatus. In addition, nine taxa of Hypoxylon are reported for Argentina for the first time, and some details on their asexual state and stromatal secondary metabolites are reported. An updated dichotomous key for Hypoxylon species from Argentina is provided.
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Lenormandins A—G, new azaphilones from Hypoxylon lenormandii and Hypoxylon jaklitschii sp. nov., recognised by chemotaxonomic data
Fungal Diversity, 2015Co-Authors: Eric Kuhnert, Frank Surup, Christopher Lambert, Adriana I Hladki, Andrea I Romero, Kevin D Hyde, Marc StadlerAbstract:Seven new azaphilone pigments named lenormandins A–G were isolated from stromata (fruiting bodies) of the xylariaceous fungus, Hypoxylon lenormandii using preparative High Performance Liquid Chromatography (HPLC) and their structures were elucidated by NMR spectroscopy, high resolution mass spectrometry and other spectral methods. Their occurrence in herbarium specimens (including various type materials collected in the 19th and early 20th century) and several fresh collections was studied by analytical HPLC with diode array and mass spectrometric detection (HPLC-DAD/MS), revealing that this group of pigments is specific for Hypoxylon lenormandii from various geographic regions and otherwise only occurs in closely related taxa, i.e. H. sublenormandii and an undescribed fungus from Sri Lanka, for which we propose the name H. jaklitschii . The status of the new species was established by using molecular phylogenetic data based on ITS and partial beta-tubulin sequences, and by detailed morphological studies.
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lenormandins a g new azaphilones from Hypoxylon lenormandii and Hypoxylon jaklitschii sp nov recognised by chemotaxonomic data
Fungal Diversity, 2015Co-Authors: Eric Kuhnert, Frank Surup, Christopher Lambert, Adriana I Hladki, Andrea I Romero, Kevin D Hyde, Marc StadlerAbstract:Seven new azaphilone pigments named lenormandins A–G were isolated from stromata (fruiting bodies) of the xylariaceous fungus, Hypoxylon lenormandii using preparative High Performance Liquid Chromatography (HPLC) and their structures were elucidated by NMR spectroscopy, high resolution mass spectrometry and other spectral methods. Their occurrence in herbarium specimens (including various type materials collected in the 19th and early 20th century) and several fresh collections was studied by analytical HPLC with diode array and mass spectrometric detection (HPLC-DAD/MS), revealing that this group of pigments is specific for Hypoxylon lenormandii from various geographic regions and otherwise only occurs in closely related taxa, i.e. H. sublenormandii and an undescribed fungus from Sri Lanka, for which we propose the name H. jaklitschii. The status of the new species was established by using molecular phylogenetic data based on ITS and partial beta-tubulin sequences, and by detailed morphological studies.
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novedades para los generos annuloHypoxylon e Hypoxylon ascomycota xylariaceae en la republica argentina
Darwiniana, 2009Co-Authors: Adriana I Hladki, Andrea I RomeroAbstract:Hladki, A. I. & A. I. Romero. 2009. Novelties for the genera AnnuloHypoxylon and Hypoxylon (Ascomy- cota, Xylariaceae) from Argentina. Darwiniana 47(2): 278-288. Two new varieties, AnnuloHypoxylon moriforme var. macrosporum and Hypoxylon investiens var. magnisporum are proposed; AnnuloHypoxylon nitens, Hypoxylon crocopeplum, H. subrutilum and H. rubiginosum var. microsporum are described as new records from Argentina. A dichotomous key to hypoxyloid taxa so far known from Argentina is presented.
