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Jose Galberto Martins Da Costa - One of the best experts on this subject based on the ideXlab platform.
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HPLC-DAD analysis and antifungal effect of Hyptis martiusii Benth (Lamiaceae) against Candida strains
Wolters Kluwer Medknow Publications, 2019Co-Authors: Janaína Esmeraldo Rocha, Irwin Rose Alencar De Menezes, Jose Galberto Martins Da Costa, Henrique Douglas Melo Coutinho, Cícero Roberto Nascimento Saraiva, Antonia Thassya Lucas Dos Santos, Antônio Judson Targino Machado, João Tavares Calixto Júnior, Jaime Ribeiro-filho, Aracelio Viana ColaresAbstract:Objective: To evaluate the anti Candida activity of Hyptis martiusii decoction and its major compound, caffeic acid alone or in the presence of fluconazole, as well as their cytotoxic effect. Methods: The decoction was characterized using high performance liquid chromatography coupled with diode array detector. For the antifungal activity, the minimum inhibitory concentration (MIC) and the potential effect of the decoction with the fluconazole were evaluated by microdilution method using 96-well microtiter trays. The osmotic fragility test was performed using erythrocytes under saline stress. All tests were performed in triplicate. Results: The chemical characterization of the decoction was performed by high performance liquid chromatography and revealed the presence of seven compounds, including caffeic acid as major constituent. The antifungal tests demonstrated that both decoction (DHm) and caffeic acid obtained from Hyptis martiusii presented MIC and MFC ≥ 4096 μg/mL against Candida albicans and Candida tropicalis strains. However, in the presence of fluconazole, DHm and caffeic acid presented IC50 of 2.60 and 2.53 μg/mL respectively, demonstrating significant synergistic effects against Candida strains. The modulator activity of DHm might be due to the presence of caffeic acid. Moreover, DHm and caffeic acid did not cause significant hemolytic effects, indicating that they present low cytotoxicity. Conclusions: These data indicate that DHm potentiates the activity of the fluconazole, without enhancement of the toxicity, encouraging further toxicological, pharmacological and phytochemical studies to provide consistent evidence of the potential of this plant to be used in drug development
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gastroprotective and ulcer healing effects of essential oil of Hyptis martiusii benth lamiaceae
PLOS ONE, 2014Co-Authors: Germana Freire Rocha Caldas, Alisson Rodrigo Da Silva Oliveira, Alice Valenca Araujo, Dafne Carolina Alves Quixabeira, Jacinto Da Costa Silvaneto, Joao Henrique Costasilva, Irwin Rose Alencar De Menezes, Fabiano Ferreira, Ana Cristina Lima Leite, Jose Galberto Martins Da CostaAbstract:Hyptis martiusii Benth. is an aromatic plant found in abundance in northeastern Brazil that is used in ethnomedicine to treat gastric disorders. The aim of this study was to elucidate the mechanisms of action involved in the gastroprotection of the essential oil of Hyptis martiusii (EOHM) and to evaluate its healing capacity. Wistar rats were exposed to different protocols and subsequently were treated with 1% Tween-80 aqueous solution (negative control), pantoprazole, carbenoxolone, N-acetylcysteine (depending on the specificity of each model) or EOHM. The antisecretory activity (basal or stimulated) was determined using the pyloric ligature method. The gastroprotective action of nitric oxide and sulphydryl groups (–SH groups), as well as the quantification of adherent mucus and the levels of malondialdehyde and –SH groups in gastric mucosa, were evaluated using ethanol-induced gastric ulcer model. The healing ability was evaluated using the acetic acid-induced gastric ulcer model and histological and immunohistochemical analysis (HE, PAS and PCNA). EOHM (400 mg/kg) reduced the volume and acidity of gastric secretion stimulated by histamine and pentagastrin. The gastroprotective effect of EOHM involves the participation of endogenous sulfhydryl groups. EOHM increased mucus production (54.8%), reduced levels of MDA (72.5%) and prevented the depletion of –SH groups (73.8%) in the gastric mucosa. The treatment with EOHM reduced in 70.3% the gastric lesion area, promoting significant regeneration of the gastric mucosa, as confirmed by histological analysis and analysis of proliferating cell nuclear antigen. The results show that gastroprotective effect of EOHM is mediated by cytoprotective and antioxidant mechanisms and by their antisecretory activity, and suggest that the essential oil of Hyptis martiusii is a promising candidate for the treatment of gastric ulcers.
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antiulcerogenic activity of the essential oil of Hyptis martiusii benth lamiaceae
Journal of Ethnopharmacology, 2011Co-Authors: Germana Freire Rocha Caldas, Jose Galberto Martins Da Costa, Iggor Macedo Do Amaral Costa, Juciene Bezerra Rodrigues Da Silva, Rafaella Farias Da Nobrega, Fabiola Fernandes Galvao Rodrigues, Almir Goncalves WanderleyAbstract:Abstract Ethnopharmacological relevance Hyptis martiusii (Lamiaceae), popularly known as “cidreira-do-mato” or “cidreira-brava”, grows in abundance in the Northeast region of Brazil, where its leaves are traditionally used in folk medicine in the treatment of intestinal and stomach diseases. The aim of this study was to characterize the chemical constituents and to evaluate the anti-ulcerogenic activity of the essential oil of the leaves of Hyptis martiusii (EOHM) in in vivo models of experimental ulcers in rodents. Materials and methods EOHM was obtained by hydrodistillation and analyzed by gas chromatography–mass spectrometry (GC–MS). Acute gastric ulcer was induced using absolute ethanol, HCl/ethanol and indomethacin. The volume, pH and total acidity of gastric secretion were determined by the pyloric ligature method and gastrointestinal motility using gastric emptying and intestinal transit. Results Chemical analysis revealed the presence of 24 components that account for 92.13% of the essential oil of dried leaves, indicating the occurrence of mono and sesquiterpenes. Oral administration of EOHM (100, 200 and 400 mg/kg) inhibited ethanol-, HCl/ethanol- and indomethacin-induced ulcers. In the case of pylorus ligature, the oil reduced the volume of gastric juices and total acidity, and increased gastric pH. The EOHM reduced the rate of gastric emptying with only the highest doses, but did not show any effect on intestinal transit at any of the three doses. Conclusions The results indicate that the essential oil of leaves of Hyptis martiusii has an antiulcerogenic activity, as evidenced by its significant inhibition of the formation of ulcers in various models. This effect could be related to an increase of gastric mucosal defensive factors. Further pharmacological studies are being undertaken in order to provide more precise elucidation of the action mechanism involved in this activity.
Hideshi Inoue - One of the best experts on this subject based on the ideXlab platform.
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a new bis seco abietane diterpenoid from Hyptis crenata pohl ex benth
ChemInform, 2015Co-Authors: Haruhiko Fukaya, Takahisa Nakane, Akihito Takano, Shigeru Takahashi, Yuji Takahashi, Hideshi InoueAbstract:The new bis-seco-abietane diterpenoid named Hyptisolide A (I) is isolated from the methanolic extracts of the aereal parts of Hyptis crenata Pohl ex Benth.
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a new bis seco abietane diterpenoid from Hyptis crenata pohl ex benth
Organic Letters, 2014Co-Authors: Haruhiko Fukaya, Takahisa Nakane, Akihito Takano, Shigeru Takahashi, Yuji Takahashi, Hideshi InoueAbstract:A new, highly oxidized, bis-seco-abietane diterpenoid named Hyptisolide A (1) was isolated from Hyptis crenata Pohl ex Benth. Its structure and stereochemistry were elucidated on the basis of data obtained by HRESIMS, NMR, and X-ray diffraction analyses, and its absolute configuration was determined with vibrational circular dichroism spectroscopy. By reporter gene assay, 1 was demonstrated to induce cAMP-responsive element-dependent transcription in Neuro2A cells.
Germana Freire Rocha Caldas - One of the best experts on this subject based on the ideXlab platform.
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gastroprotective and ulcer healing effects of essential oil of Hyptis martiusii benth lamiaceae
PLOS ONE, 2014Co-Authors: Germana Freire Rocha Caldas, Alisson Rodrigo Da Silva Oliveira, Alice Valenca Araujo, Dafne Carolina Alves Quixabeira, Jacinto Da Costa Silvaneto, Joao Henrique Costasilva, Irwin Rose Alencar De Menezes, Fabiano Ferreira, Ana Cristina Lima Leite, Jose Galberto Martins Da CostaAbstract:Hyptis martiusii Benth. is an aromatic plant found in abundance in northeastern Brazil that is used in ethnomedicine to treat gastric disorders. The aim of this study was to elucidate the mechanisms of action involved in the gastroprotection of the essential oil of Hyptis martiusii (EOHM) and to evaluate its healing capacity. Wistar rats were exposed to different protocols and subsequently were treated with 1% Tween-80 aqueous solution (negative control), pantoprazole, carbenoxolone, N-acetylcysteine (depending on the specificity of each model) or EOHM. The antisecretory activity (basal or stimulated) was determined using the pyloric ligature method. The gastroprotective action of nitric oxide and sulphydryl groups (–SH groups), as well as the quantification of adherent mucus and the levels of malondialdehyde and –SH groups in gastric mucosa, were evaluated using ethanol-induced gastric ulcer model. The healing ability was evaluated using the acetic acid-induced gastric ulcer model and histological and immunohistochemical analysis (HE, PAS and PCNA). EOHM (400 mg/kg) reduced the volume and acidity of gastric secretion stimulated by histamine and pentagastrin. The gastroprotective effect of EOHM involves the participation of endogenous sulfhydryl groups. EOHM increased mucus production (54.8%), reduced levels of MDA (72.5%) and prevented the depletion of –SH groups (73.8%) in the gastric mucosa. The treatment with EOHM reduced in 70.3% the gastric lesion area, promoting significant regeneration of the gastric mucosa, as confirmed by histological analysis and analysis of proliferating cell nuclear antigen. The results show that gastroprotective effect of EOHM is mediated by cytoprotective and antioxidant mechanisms and by their antisecretory activity, and suggest that the essential oil of Hyptis martiusii is a promising candidate for the treatment of gastric ulcers.
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antiulcerogenic activity of the essential oil of Hyptis martiusii benth lamiaceae
Journal of Ethnopharmacology, 2011Co-Authors: Germana Freire Rocha Caldas, Jose Galberto Martins Da Costa, Iggor Macedo Do Amaral Costa, Juciene Bezerra Rodrigues Da Silva, Rafaella Farias Da Nobrega, Fabiola Fernandes Galvao Rodrigues, Almir Goncalves WanderleyAbstract:Abstract Ethnopharmacological relevance Hyptis martiusii (Lamiaceae), popularly known as “cidreira-do-mato” or “cidreira-brava”, grows in abundance in the Northeast region of Brazil, where its leaves are traditionally used in folk medicine in the treatment of intestinal and stomach diseases. The aim of this study was to characterize the chemical constituents and to evaluate the anti-ulcerogenic activity of the essential oil of the leaves of Hyptis martiusii (EOHM) in in vivo models of experimental ulcers in rodents. Materials and methods EOHM was obtained by hydrodistillation and analyzed by gas chromatography–mass spectrometry (GC–MS). Acute gastric ulcer was induced using absolute ethanol, HCl/ethanol and indomethacin. The volume, pH and total acidity of gastric secretion were determined by the pyloric ligature method and gastrointestinal motility using gastric emptying and intestinal transit. Results Chemical analysis revealed the presence of 24 components that account for 92.13% of the essential oil of dried leaves, indicating the occurrence of mono and sesquiterpenes. Oral administration of EOHM (100, 200 and 400 mg/kg) inhibited ethanol-, HCl/ethanol- and indomethacin-induced ulcers. In the case of pylorus ligature, the oil reduced the volume of gastric juices and total acidity, and increased gastric pH. The EOHM reduced the rate of gastric emptying with only the highest doses, but did not show any effect on intestinal transit at any of the three doses. Conclusions The results indicate that the essential oil of leaves of Hyptis martiusii has an antiulcerogenic activity, as evidenced by its significant inhibition of the formation of ulcers in various models. This effect could be related to an increase of gastric mucosal defensive factors. Further pharmacological studies are being undertaken in order to provide more precise elucidation of the action mechanism involved in this activity.
Haruhiko Fukaya - One of the best experts on this subject based on the ideXlab platform.
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a new bis seco abietane diterpenoid from Hyptis crenata pohl ex benth
ChemInform, 2015Co-Authors: Haruhiko Fukaya, Takahisa Nakane, Akihito Takano, Shigeru Takahashi, Yuji Takahashi, Hideshi InoueAbstract:The new bis-seco-abietane diterpenoid named Hyptisolide A (I) is isolated from the methanolic extracts of the aereal parts of Hyptis crenata Pohl ex Benth.
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a new bis seco abietane diterpenoid from Hyptis crenata pohl ex benth
Organic Letters, 2014Co-Authors: Haruhiko Fukaya, Takahisa Nakane, Akihito Takano, Shigeru Takahashi, Yuji Takahashi, Hideshi InoueAbstract:A new, highly oxidized, bis-seco-abietane diterpenoid named Hyptisolide A (1) was isolated from Hyptis crenata Pohl ex Benth. Its structure and stereochemistry were elucidated on the basis of data obtained by HRESIMS, NMR, and X-ray diffraction analyses, and its absolute configuration was determined with vibrational circular dichroism spectroscopy. By reporter gene assay, 1 was demonstrated to induce cAMP-responsive element-dependent transcription in Neuro2A cells.
Almir Goncalves Wanderley - One of the best experts on this subject based on the ideXlab platform.
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antiulcerogenic activity of the essential oil of Hyptis martiusii benth lamiaceae
Journal of Ethnopharmacology, 2011Co-Authors: Germana Freire Rocha Caldas, Jose Galberto Martins Da Costa, Iggor Macedo Do Amaral Costa, Juciene Bezerra Rodrigues Da Silva, Rafaella Farias Da Nobrega, Fabiola Fernandes Galvao Rodrigues, Almir Goncalves WanderleyAbstract:Abstract Ethnopharmacological relevance Hyptis martiusii (Lamiaceae), popularly known as “cidreira-do-mato” or “cidreira-brava”, grows in abundance in the Northeast region of Brazil, where its leaves are traditionally used in folk medicine in the treatment of intestinal and stomach diseases. The aim of this study was to characterize the chemical constituents and to evaluate the anti-ulcerogenic activity of the essential oil of the leaves of Hyptis martiusii (EOHM) in in vivo models of experimental ulcers in rodents. Materials and methods EOHM was obtained by hydrodistillation and analyzed by gas chromatography–mass spectrometry (GC–MS). Acute gastric ulcer was induced using absolute ethanol, HCl/ethanol and indomethacin. The volume, pH and total acidity of gastric secretion were determined by the pyloric ligature method and gastrointestinal motility using gastric emptying and intestinal transit. Results Chemical analysis revealed the presence of 24 components that account for 92.13% of the essential oil of dried leaves, indicating the occurrence of mono and sesquiterpenes. Oral administration of EOHM (100, 200 and 400 mg/kg) inhibited ethanol-, HCl/ethanol- and indomethacin-induced ulcers. In the case of pylorus ligature, the oil reduced the volume of gastric juices and total acidity, and increased gastric pH. The EOHM reduced the rate of gastric emptying with only the highest doses, but did not show any effect on intestinal transit at any of the three doses. Conclusions The results indicate that the essential oil of leaves of Hyptis martiusii has an antiulcerogenic activity, as evidenced by its significant inhibition of the formation of ulcers in various models. This effect could be related to an increase of gastric mucosal defensive factors. Further pharmacological studies are being undertaken in order to provide more precise elucidation of the action mechanism involved in this activity.