The Experts below are selected from a list of 26784813 Experts worldwide ranked by ideXlab platform
David B. Archer - One of the best experts on this subject based on the ideXlab platform.
-
Development of an unmarked gene deletion system for the filamentous fungi [i]Aspergillus niger[/i] and [i]Talaromyces versatilis[/i]
Applied and Environmental Microbiology, 2014Co-Authors: Stephane Delmas, Augustina Llanos, Jean-luc Parrou, Matthew Kokolski, Steven T. Pullan, Lee Shunburne, David B. ArcherAbstract:In this article, we present a method to delete genes in filamentous fungi that allows recycling of the selection marker and is efficient in a nonhomologous end-joining (NHEJ)-proficient strain. We exemplify the approach I?By deletion of the gene encoding the transcriptional regulator XlnR in the fungus Aspergillus niger. To show the efficiency and advantages of the method, we deleted 8 other genes and constructed a douI?Ble mutant in this species. Moreover, we showed that the same principle also functions in a different genus of filamentous fungus (Talaromyces versatilis, I?Basionym Penicillium funiculosum). This technique will increase the versatility of the toolI?Boxes for genome manipulation of model and industrially relevant fungi.
Stephane Delmas - One of the best experts on this subject based on the ideXlab platform.
-
Development of an unmarked gene deletion system for the filamentous fungi [i]Aspergillus niger[/i] and [i]Talaromyces versatilis[/i]
Applied and Environmental Microbiology, 2014Co-Authors: Stephane Delmas, Augustina Llanos, Jean-luc Parrou, Matthew Kokolski, Steven T. Pullan, Lee Shunburne, David B. ArcherAbstract:In this article, we present a method to delete genes in filamentous fungi that allows recycling of the selection marker and is efficient in a nonhomologous end-joining (NHEJ)-proficient strain. We exemplify the approach I?By deletion of the gene encoding the transcriptional regulator XlnR in the fungus Aspergillus niger. To show the efficiency and advantages of the method, we deleted 8 other genes and constructed a douI?Ble mutant in this species. Moreover, we showed that the same principle also functions in a different genus of filamentous fungus (Talaromyces versatilis, I?Basionym Penicillium funiculosum). This technique will increase the versatility of the toolI?Boxes for genome manipulation of model and industrially relevant fungi.
Hariwan Zikri Ibrahim - One of the best experts on this subject based on the ideXlab platform.
-
(i;j)- -I-i-Open Sets and (i;j)- -I-i-Almost Continuous Functions in Ideal I?Bitopological Spaces
2014Co-Authors: Hariwan Zikri IbrahimAbstract:In this paper we introduce and study the notion of (i, j)-β-I-i-open sets in ideal I?Bitopological spaces and investigate some of their properties. Also we present and study (i, j)-β-I-i-continuous and (i, j)-β-I- i-almost continuous functions. Furthermore, we oI?Btain I?Basic properties and preservation theorems of (i, j)-β-I- i-continuous and (i, j)-β-I-i-almost continuous.
-
(i,j)-β-I-i-Open Sets and (i,j)-β-I-i-Almost Continuous Functions in Ideal I?Bitopological Spaces
Universal Journal of Applied Mathematics, 2014Co-Authors: Hariwan Zikri IbrahimAbstract:In this paper we introduce and study the notion of (i, j)-β-I-i-open sets in ideal I?Bitopological spaces and investigate some of their properties. Also we present and study (i, j)-β-I-i-continuous and (i, j)-β-Ii- almost continuous functions. Furthermore, we oI?Btain I?Basic properties and preservation theorems of (i, j)-β-I-i-continuous and (i, j)-β-I-i-almost continuous.
Matthew Kokolski - One of the best experts on this subject based on the ideXlab platform.
-
Development of an unmarked gene deletion system for the filamentous fungi [i]Aspergillus niger[/i] and [i]Talaromyces versatilis[/i]
Applied and Environmental Microbiology, 2014Co-Authors: Stephane Delmas, Augustina Llanos, Jean-luc Parrou, Matthew Kokolski, Steven T. Pullan, Lee Shunburne, David B. ArcherAbstract:In this article, we present a method to delete genes in filamentous fungi that allows recycling of the selection marker and is efficient in a nonhomologous end-joining (NHEJ)-proficient strain. We exemplify the approach I?By deletion of the gene encoding the transcriptional regulator XlnR in the fungus Aspergillus niger. To show the efficiency and advantages of the method, we deleted 8 other genes and constructed a douI?Ble mutant in this species. Moreover, we showed that the same principle also functions in a different genus of filamentous fungus (Talaromyces versatilis, I?Basionym Penicillium funiculosum). This technique will increase the versatility of the toolI?Boxes for genome manipulation of model and industrially relevant fungi.
Jean-luc Parrou - One of the best experts on this subject based on the ideXlab platform.
-
Development of an unmarked gene deletion system for the filamentous fungi [i]Aspergillus niger[/i] and [i]Talaromyces versatilis[/i]
Applied and Environmental Microbiology, 2014Co-Authors: Stephane Delmas, Augustina Llanos, Jean-luc Parrou, Matthew Kokolski, Steven T. Pullan, Lee Shunburne, David B. ArcherAbstract:In this article, we present a method to delete genes in filamentous fungi that allows recycling of the selection marker and is efficient in a nonhomologous end-joining (NHEJ)-proficient strain. We exemplify the approach I?By deletion of the gene encoding the transcriptional regulator XlnR in the fungus Aspergillus niger. To show the efficiency and advantages of the method, we deleted 8 other genes and constructed a douI?Ble mutant in this species. Moreover, we showed that the same principle also functions in a different genus of filamentous fungus (Talaromyces versatilis, I?Basionym Penicillium funiculosum). This technique will increase the versatility of the toolI?Boxes for genome manipulation of model and industrially relevant fungi.
