The Experts below are selected from a list of 165 Experts worldwide ranked by ideXlab platform
Ralph E Schrohenloher - One of the best experts on this subject based on the ideXlab platform.
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expression of rheumatoid factor Idiotypes 17 109 6b6 6 and 4c9 in the sera of pima indians
Autoimmunity, 1994Co-Authors: Anne Davidson, Ralph E Schrohenloher, Harold Keiser, Antonio Del Puente, Peter H Bennett, William J KoopmanAbstract:This study was undertaken to determine whether the expression of 17.109, 6B6.6 and 4C9 rhematoid factor (RF) Idiotypes is predictive of the development of rheumatoid arthritis (RA) and whether the RF response is idiotypically restricted in an inbred population of Pima Indians who have a genetic predisposition for the disease.Serial sera were obtained from 25 subjects who developed RA and 25 RF-positive subjects who did not develop RA over the course of a longitudinal community health survey. RF titers and titers of the RF-associated Idiotypes 17.109, 6B6.6 and 4C9 were determined by ELISA, and the relationship between 6B6.6 and 4C9 was analyzed by cross-absorption studies.Expression of the three RF-associated Idiotypes was found in both the subjects who developed RA and those who did not. The amount of idiotype expressed was variable, but a few subjects in both groups had high levels indicative of an oligoclonal RF response. Reactivity with 6B6.6 and 4C9 antiIdiotypes overlapped, with 4C9 appearing to mar...
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shared Idiotypes in mesangial deposits in iga nephropathy are not disease specific
Kidney International, 1993Co-Authors: Warmold A L Van Den Wall Bake, Jan A Bruijn, Mary Ann Accavitti, Peggy A Crowleynowick, Ralph E SchrohenloherAbstract:Shared Idiotypes in mesangial deposits in IgA nephropathy are not disease-specific. The antigenic specificity of the mesangial IgA in IgA nephropathy (IgAN) remains unknown. Because shared antigenic specificities may be reflected in the usage of shared Idiotypes, we prepared five monoclonal anti-idiotypic antibodies (MoAbs) specific for the mesangial IgA eluted from the kidney of an IgAN patient. All five MoAbs reacted with the same idiotype, which proved to be of a public nature. Although the idiotype could be identified in the mesangial deposits of the majority of IgAN patients studied, it was not specific for the disease because it was also found in the glomerular deposits of other types of glomerulonephritis. The idiotype was also expressed in polyethylene glycol precipitates of sera and in pokeweed mitogen-induced plasma cells from both IgAN patients and healthy controls. The conclusion that no disease-specific Idiotypes are present in the renal eluate was further supported by the failure to produce polyclonal anti-idiotypic antibodies by immunizing a rabbit with the eluted mesangial IgA. Our results support the concept that mesangial IgA deposits in IgAN are of a polyclonal nature.
John H Vaughan - One of the best experts on this subject based on the ideXlab platform.
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occurrence of two germline related rheumatoid factor Idiotypes in rheumatoid arthritis and in non rheumatoid seropositive individuals
Clinical and Experimental Immunology, 2008Co-Authors: T Kouri, J Crowley, K Aho, T Palosuo, H Isomaki, R Essen, M Heliovaara, Dennis A Carson, John H VaughanAbstract:Human rheumatoid factor (RF) paraproteins express two distinct light chain cross-reactive Idiotypes defined by the monoclonal antibodies 17.109 and 6B6.6. These germline gene-related cross-reactive Idiotypes are both carried on VK3 light chains and are each present on about one-third of IgM RF paraproteins. We assessed the degree to which these Idiotypes are represented in polyclonal RFs. We used rheumatoid arthritis (RA) and non-RA RF-positive sera selected from a large cross-sectional population study (the Mini-Finland Health Survey), and sera from a community-based follow-up study of recent-onset RA patients from Heinola, Finland. In the Mini-Finland Health Survey, elevated levels of the 17.109 RF idiotype were seen in sera of 13% of the RA and 19% of the non-RA group; 6B6.6 RF was seen in 26% of the RA and 28% of the non-RA group. In sera of the Heinola follow-up study, 17.109 RF was seen in 12% initially, but in only 3% at 8 years. Similarly, 6B6.6 RF was detected in 25% initially, but in only 7% at 8 years. Ten sera positive for RF prior to the onset of clinical RA were identified from individuals of a second large population study from Finland (North Karelia project); two of these sera exhibited the 6B6.6 idiotype; none exhibited the 17.109 idiotype. The data are consistent with the concept that these germline gene-related cross-reactive RF Idiotypes occur frequently in the polyclonal RF of non-RA as well as RA sera, and that in RA the Idiotypes may sometimes be reduced or lost as a consequence of somatic diversification of the RF through somatic mutation, usage of new germline genes, or both.
Nigel H. Russell - One of the best experts on this subject based on the ideXlab platform.
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Generation of anti‐idiotype immune responses following vaccination with idiotype‐protein pulsed dendritic cells in myeloma
British journal of haematology, 1999Co-Authors: G.m. Cull, L Durrant, C Stainer, A P Haynes, Nigel H. RussellAbstract:Myeloma cells produce immunoglobulin which is unique to the malignant clone and presents antigenic determinants, or Idiotypes, which may function as a tumour-specific antigen. The availability of significant quantities of idiotype protein in the serum makes immunotherapeutic strategies utilizing this protein to generate an anti-idiotype immune response an attractive prospect. We treated two patients with advanced refractory myeloma with a series of four vaccinations using autologous idiotype-protein pulsed dendritic cells combined with adjuvant GM-CSF. The vaccinations were well tolerated with a mild fever post-vaccination in one patient. An idiotype-specific T-cell proliferative response developed in both patients. This T-cell response was associated with the production of γ-interferon, indicating a TH-1-like response. Furthermore, one patient developed anti-idiotype IgM antibodies. However, no idiotype-specific cytotoxic T-cell response could be demonstrated. Further investigation is warranted to define the optimal conditions for dendritic cell culture and priming to maximize the anti-tumour immune response.
William J Koopman - One of the best experts on this subject based on the ideXlab platform.
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expression of rheumatoid factor Idiotypes 17 109 6b6 6 and 4c9 in the sera of pima indians
Autoimmunity, 1994Co-Authors: Anne Davidson, Ralph E Schrohenloher, Harold Keiser, Antonio Del Puente, Peter H Bennett, William J KoopmanAbstract:This study was undertaken to determine whether the expression of 17.109, 6B6.6 and 4C9 rhematoid factor (RF) Idiotypes is predictive of the development of rheumatoid arthritis (RA) and whether the RF response is idiotypically restricted in an inbred population of Pima Indians who have a genetic predisposition for the disease.Serial sera were obtained from 25 subjects who developed RA and 25 RF-positive subjects who did not develop RA over the course of a longitudinal community health survey. RF titers and titers of the RF-associated Idiotypes 17.109, 6B6.6 and 4C9 were determined by ELISA, and the relationship between 6B6.6 and 4C9 was analyzed by cross-absorption studies.Expression of the three RF-associated Idiotypes was found in both the subjects who developed RA and those who did not. The amount of idiotype expressed was variable, but a few subjects in both groups had high levels indicative of an oligoclonal RF response. Reactivity with 6B6.6 and 4C9 antiIdiotypes overlapped, with 4C9 appearing to mar...
Yehuda Shoenfeld - One of the best experts on this subject based on the ideXlab platform.
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anti dna Idiotypes from induction of disease to novel therapeutical approaches
Immunology Letters, 2005Co-Authors: Yehuda ShoenfeldAbstract:Intravenous polyclonal immunoglobulins (IVIG) were used as a therapy of autoimmune diseases and especially in conventional therapy resistance cases of SLE. The main mechanism by which IVIG exert its effect in autoimmunityentails the existence of specific anti-idiotypic antibodies in the compound against itiotypes of autoantibodies. Pathogenic Idiotypes of autoantibodies were employed in the past to induce experimental autoimmune diseases via active immunization. Specific IVIG (sIVIG) was constructed from a commercial IVIG absorption on a column of pathogenic idiotype and elution of the bound following immunoglobulins. The sIVIG was found to be more effective than IVIG in suppressing murine SLE. Peptides fished out from a library via the sIVIG were found to be effective in replacing the Idiotypes of anti-DNA antibodies. sIVIG absorbed on a Id peptides column, were found to be as effective in experimental murine SLE as sIVIG generated on an Id column. The "fished" Id peptides enable the commercial production of sIVIG. In parallel peptides derived from a pathogenic idiotype were also employed for therapeutical purposes in human phase I, II studies and suppressed SLE following active immunization. Thus, the idiotypic network proposed by Jeme in 1974 led to at least two novel therapeutic avenues in autoimmunity, by and large and in SLE specifically.
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Idiotypes in medicine autoimmunity infection and cancer
1997Co-Authors: Yehuda Shoenfeld, Ronald C Kennedy, Soldano FerroneAbstract:Part 1 General section: anti-Idiotypes as vaccines - theoretical considerations anti-Idiotypes x-ray crystallographic studies of the idiotypic cascade the structure and thermodynamics of antibody-protein antigen interactions. Part 2 Autoantibody section: Idiotypes and anti-Idiotypes cross-reactive Idiotypes in human lupus families Idiotypes and anti-DNA antibodies Idiotypes of anti-sm antibodies Idiotypes of anti-ro and anti-la Idiotypes of autoantibodies in myasthenia gravis Idiotypes of ANCA Ig genes and idiotype of anti-mitochondrial antibodies in primary biliary cirrhosis Idiotypes of anti-phospholipid antibodies anti-idiotypic antibodies against human insulin autoantibodies (IAA) molecular and cellular aspects of human rheumatoid factor production and Idiotypes Idiotypes of anti-thyroglobulin antibodies Idiotypes of human platelet-specific antibodies Idiotypes of platelet associated antibodies (autoimmune thrombocytopenia) Idiotypes of natural autoantibodies and monoclonal gammopathies Idiotypes and anti-Idiotypes in allergy anti-Idiotypes to autoantibodies in therapeutic preparations of normal polyspecific human IgG (intravenous immunoglobulin, IVIg) manipulation of autoantibody Idiotypes in autoimmune diseases. Part 3 Infection section: introduction anti-idiotypic studies of the glycoprotine B(gB,UL55) complex of human cytomegalovirus - mimicry of a neutralizing epitope and development of diagnostics anti-idiotype antibody in HIV-1-infected thrombocytopenic patients an electron microscopic view of the geometry and dynamics of idiotype-anti-idiotype interactions probing human antibody diversity in HIV infection anti-idiotype mimics of "pseudomonas aeruginosa" surface polysaccharides modulation of the immune response to simian virus 40 large tumor antigen via idiotype-anti-idiotype interactions the perpetual network theory and its putative applications - 21 years later an anti-idiotypic antibody as a functional mirror image of a viral antigen anti-idiotype to bluetongue virus VP7 antigen - potential diagnostic reagent and vaccine an idiotype vaccine against gram-negative bacterial sepsis yeast killer toxin-like antibodies in experimental and clinical candidiasis anti-idiotypic antibodies and idiotypic T cells selections in HIV infection - 1 - Idiotypes of HIV specific antibodies as disease markers in AIDS and their potential as immunotherapeutic agents, 2 - T-cell repertoire and Idiotypes in HIV infection probimg human polysaccharide-specific antibody repertoires using anti-Idiotypes anti-idiotypic antibodies in HIV-infected patients to a neutralizing mab and its CDRs (complementarity determining regions) Epstein-Barr virus-induced autoimmunity through anti-idiotype studies. (Part contents)
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Idiotypes and Anti-Idiotypic Antibodies
Autoantibodies, 1996Co-Authors: Mahmoud Abu-shakra, Dan Buskila, Yehuda ShoenfeldAbstract:Publisher Summary This chapter discusses the Idiotypes and anti-idiotypic antibodies. Intensive efforts to standardize the assay, define associations with disease or clinical presentation, and characterize antigenic and biological properties of these antibodies are included. Idiotypic dysregulation have a significant role in the pathogenesis of autoimmune diseases. Various experimental models of autoimmune diseases including systemic lupus erythematosus (SLE), antiphospholipid syndrome and Wegener's granulomatosis (WG) were induced in naive mice following idiotypic immunization. Idiotypes, are the antigenic determinants of immunoglobulin molecules that are located in the variable region of the antibodies. High levels of pathogenic Idiotypes detected in the sera of patients with autoimmune diseases include the pathogenic anti-DNA idiotype 16/6 found in the sera of patients with active SLE. Under normal conditions, Idiotypes and their antiidiotypic antibodies have a major role in regulating the immune response to self and foreign antigens.
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Anti-Idiotypes and their application under autoimmune, neoplastic, and infectious conditions
International archives of allergy and immunology, 1994Co-Authors: Yehuda Shoenfeld, Soldano Ferrone, Howard Amital, Ronald C KennedyAbstract:Idiotypes and anti-Idiotypes are becoming widely used in various experimental applications in multiple fields of research. Administration of anti-Idiotypes has been attempted in order to manipulate immune reactions affecting autoimmune, neoplastic, and infectious processes. Immunization with anti-Idiotypes and Idiotypes may change the autoantibody expression in autoimmune diseases. In certain experimental models administration of anti-Idiotypes and Idiotypes may even propagate the induction of autoimmune-like disorders. Internal image bearing anti-Idiotypes may provide an effective tool in the combat of neoplastic and infectious diseases. Such anti-Idiotypes may resemble the structure of neoplastic cell antigen or mimic the composition of a contagious viral antigen. This review will deal in detail with the various aspects of idiotype and anti-idiotype immunomanipulation.
