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Mathias Kolle - One of the best experts on this subject based on the ideXlab platform.

  • luminescent surfaces with tailored angular emission for compact dark field imaging devices
    Nature Photonics, 2020
    Co-Authors: Cecile A C Chazot, Sara Nagelberg, Christopher J Rowlands, Maik R J Scherer, Igor Coropceanu, Kurt Broderick, Yunjo Kim, Moungi G Bawendi, Mathias Kolle
    Abstract:

    Dark-field microscopy is a standard imaging technique widely employed in biology that provides high image contrast for a broad range of unstained specimens1. Unlike bright-field microscopy, it accentuates high spatial frequencies and can therefore be used to emphasize and resolve small features. However, the use of dark-field microscopy for reliable analysis of blood cells, bacteria, algae and other marine organisms often requires specialized, bulky microscope systems, as well as expensive additional components, such as dark-field-compatible objectives or condensers2,3. Here, we propose to simplify and downsize dark-field microscopy equipment by generating the high-angle Illumination Cone required for dark-field microscopy directly within the sample substrate. We introduce a luminescent photonic substrate with a controlled angular emission profile and demonstrate its ability to generate high-contrast dark-field images of micrometre-sized living organisms using standard optical microscopy equipment. This new type of substrate forms the basis for miniaturized lab-on-chip dark-field imaging devices that are compatible with simple and compact light microscopes. A luminescent photonic substrate with a controlled angular emission profile is introduced and its ability to generate high-contrast dark-field images of micrometre-sized living organisms is demonstrated using standard optical microscopy equipment.

Cecile A C Chazot - One of the best experts on this subject based on the ideXlab platform.

  • luminescent surfaces with tailored angular emission for compact dark field imaging devices
    Nature Photonics, 2020
    Co-Authors: Cecile A C Chazot, Sara Nagelberg, Christopher J Rowlands, Maik R J Scherer, Igor Coropceanu, Kurt Broderick, Yunjo Kim, Moungi G Bawendi, Mathias Kolle
    Abstract:

    Dark-field microscopy is a standard imaging technique widely employed in biology that provides high image contrast for a broad range of unstained specimens1. Unlike bright-field microscopy, it accentuates high spatial frequencies and can therefore be used to emphasize and resolve small features. However, the use of dark-field microscopy for reliable analysis of blood cells, bacteria, algae and other marine organisms often requires specialized, bulky microscope systems, as well as expensive additional components, such as dark-field-compatible objectives or condensers2,3. Here, we propose to simplify and downsize dark-field microscopy equipment by generating the high-angle Illumination Cone required for dark-field microscopy directly within the sample substrate. We introduce a luminescent photonic substrate with a controlled angular emission profile and demonstrate its ability to generate high-contrast dark-field images of micrometre-sized living organisms using standard optical microscopy equipment. This new type of substrate forms the basis for miniaturized lab-on-chip dark-field imaging devices that are compatible with simple and compact light microscopes. A luminescent photonic substrate with a controlled angular emission profile is introduced and its ability to generate high-contrast dark-field images of micrometre-sized living organisms is demonstrated using standard optical microscopy equipment.

Yunjo Kim - One of the best experts on this subject based on the ideXlab platform.

  • luminescent surfaces with tailored angular emission for compact dark field imaging devices
    Nature Photonics, 2020
    Co-Authors: Cecile A C Chazot, Sara Nagelberg, Christopher J Rowlands, Maik R J Scherer, Igor Coropceanu, Kurt Broderick, Yunjo Kim, Moungi G Bawendi, Mathias Kolle
    Abstract:

    Dark-field microscopy is a standard imaging technique widely employed in biology that provides high image contrast for a broad range of unstained specimens1. Unlike bright-field microscopy, it accentuates high spatial frequencies and can therefore be used to emphasize and resolve small features. However, the use of dark-field microscopy for reliable analysis of blood cells, bacteria, algae and other marine organisms often requires specialized, bulky microscope systems, as well as expensive additional components, such as dark-field-compatible objectives or condensers2,3. Here, we propose to simplify and downsize dark-field microscopy equipment by generating the high-angle Illumination Cone required for dark-field microscopy directly within the sample substrate. We introduce a luminescent photonic substrate with a controlled angular emission profile and demonstrate its ability to generate high-contrast dark-field images of micrometre-sized living organisms using standard optical microscopy equipment. This new type of substrate forms the basis for miniaturized lab-on-chip dark-field imaging devices that are compatible with simple and compact light microscopes. A luminescent photonic substrate with a controlled angular emission profile is introduced and its ability to generate high-contrast dark-field images of micrometre-sized living organisms is demonstrated using standard optical microscopy equipment.

Sara Nagelberg - One of the best experts on this subject based on the ideXlab platform.

  • luminescent surfaces with tailored angular emission for compact dark field imaging devices
    Nature Photonics, 2020
    Co-Authors: Cecile A C Chazot, Sara Nagelberg, Christopher J Rowlands, Maik R J Scherer, Igor Coropceanu, Kurt Broderick, Yunjo Kim, Moungi G Bawendi, Mathias Kolle
    Abstract:

    Dark-field microscopy is a standard imaging technique widely employed in biology that provides high image contrast for a broad range of unstained specimens1. Unlike bright-field microscopy, it accentuates high spatial frequencies and can therefore be used to emphasize and resolve small features. However, the use of dark-field microscopy for reliable analysis of blood cells, bacteria, algae and other marine organisms often requires specialized, bulky microscope systems, as well as expensive additional components, such as dark-field-compatible objectives or condensers2,3. Here, we propose to simplify and downsize dark-field microscopy equipment by generating the high-angle Illumination Cone required for dark-field microscopy directly within the sample substrate. We introduce a luminescent photonic substrate with a controlled angular emission profile and demonstrate its ability to generate high-contrast dark-field images of micrometre-sized living organisms using standard optical microscopy equipment. This new type of substrate forms the basis for miniaturized lab-on-chip dark-field imaging devices that are compatible with simple and compact light microscopes. A luminescent photonic substrate with a controlled angular emission profile is introduced and its ability to generate high-contrast dark-field images of micrometre-sized living organisms is demonstrated using standard optical microscopy equipment.

Moungi G Bawendi - One of the best experts on this subject based on the ideXlab platform.

  • luminescent surfaces with tailored angular emission for compact dark field imaging devices
    Nature Photonics, 2020
    Co-Authors: Cecile A C Chazot, Sara Nagelberg, Christopher J Rowlands, Maik R J Scherer, Igor Coropceanu, Kurt Broderick, Yunjo Kim, Moungi G Bawendi, Mathias Kolle
    Abstract:

    Dark-field microscopy is a standard imaging technique widely employed in biology that provides high image contrast for a broad range of unstained specimens1. Unlike bright-field microscopy, it accentuates high spatial frequencies and can therefore be used to emphasize and resolve small features. However, the use of dark-field microscopy for reliable analysis of blood cells, bacteria, algae and other marine organisms often requires specialized, bulky microscope systems, as well as expensive additional components, such as dark-field-compatible objectives or condensers2,3. Here, we propose to simplify and downsize dark-field microscopy equipment by generating the high-angle Illumination Cone required for dark-field microscopy directly within the sample substrate. We introduce a luminescent photonic substrate with a controlled angular emission profile and demonstrate its ability to generate high-contrast dark-field images of micrometre-sized living organisms using standard optical microscopy equipment. This new type of substrate forms the basis for miniaturized lab-on-chip dark-field imaging devices that are compatible with simple and compact light microscopes. A luminescent photonic substrate with a controlled angular emission profile is introduced and its ability to generate high-contrast dark-field images of micrometre-sized living organisms is demonstrated using standard optical microscopy equipment.