Image Analysis System

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Peter Baker - One of the best experts on this subject based on the ideXlab platform.

  • Disaggregation of colonies of Microcystis (Cyanobacteria): efficiency of two techniques assessed using an Image Analysis System
    Journal of Applied Phycology, 2004
    Co-Authors: Catherine Bernard, Paul Monis, Peter Baker
    Abstract:

    Disaggregation of colonies of Microcystis (Cyanobacteria) into smalleraggregates or single cells is a usefulprocess for improving the accuracy andprecision of cell abundance estimates innatural populations. An Image AnalysisSystem was therefore used to assess theefficiency of two techniques,heating/vortexing and grinding, fordisaggregating fixed and live colonies. Eight environmental samples, consisting ofeither colonial or non-colonial strains,were tested in time course experimentsusing each technique by analysing thenumber and total area of the objects (i.e.,cells, clumps of cells, colonies) acquired. The results show that i) Image Analysis isan efficient tool to compare the efficiencyof disaggregation techniques on colonies ofcyanobacteria, ii) the grinding techniqueis more efficient in disaggregating alltypes of Microcystis colony, withoutsignificant loss, and iii) the heatingtechnique does not work for all types ofcolonies, in particular, compact coloniesfixed with Lugol's iodine solution.

B. C. Fellström - One of the best experts on this subject based on the ideXlab platform.

  • Effects of angiopeptin on transplant arteriosclerosis in the rat
    Transplant International, 1995
    Co-Authors: M. L. Akyürek, A. Wanders, M. Aurivillius, E. Larsson, K. Funa, B. C. Fellström
    Abstract:

    The influence of the somatostatin analogue angiopeptin on transplant arteriosclerosis was investigated using two aortic transplantation rat models. One was characterized by ischemia/reperfusion-induced changes in syngeneic transplants while immunologically induced changes dominated in the other allogeneic model. Angiopeptin, 100 μg/kg per day, was administered continuously until the sacrifice of the rats after 8 weeks. No additional immunosuppression was used in either model. An Image Analysis System was used to quantify the intimal and medial thicknesses of the grafts. In the syngeneic grafts, the intimal thickness was less than 50% of that of control grafts ( P

Catherine Bernard - One of the best experts on this subject based on the ideXlab platform.

  • Disaggregation of colonies of Microcystis (Cyanobacteria): efficiency of two techniques assessed using an Image Analysis System
    Journal of Applied Phycology, 2004
    Co-Authors: Catherine Bernard, Paul Monis, Peter Baker
    Abstract:

    Disaggregation of colonies of Microcystis (Cyanobacteria) into smalleraggregates or single cells is a usefulprocess for improving the accuracy andprecision of cell abundance estimates innatural populations. An Image AnalysisSystem was therefore used to assess theefficiency of two techniques,heating/vortexing and grinding, fordisaggregating fixed and live colonies. Eight environmental samples, consisting ofeither colonial or non-colonial strains,were tested in time course experimentsusing each technique by analysing thenumber and total area of the objects (i.e.,cells, clumps of cells, colonies) acquired. The results show that i) Image Analysis isan efficient tool to compare the efficiencyof disaggregation techniques on colonies ofcyanobacteria, ii) the grinding techniqueis more efficient in disaggregating alltypes of Microcystis colony, withoutsignificant loss, and iii) the heatingtechnique does not work for all types ofcolonies, in particular, compact coloniesfixed with Lugol's iodine solution.

Rickard Öste - One of the best experts on this subject based on the ideXlab platform.

  • a low cost video technique for colour measurement of potato chips
    Lwt - Food Science and Technology, 1999
    Co-Authors: Sandra Segnini, Petr Dejmek, Rickard Öste
    Abstract:

    Abstract A computer-based video Image Analysis System has been developed to quantify the colour of potato chips in L * a * b * colour space. This System is not sensitive to light intensity, and the light reflection effect from the undulating surface of the chips was not significant to the colour measurement. The presence of undesirable spots on the chip surface was also quantified. The sensitivity of the technique to separate ‘colours’ correlated well with the capability of the human eye. The technique shows encouraging potential for use in studying the colour and appearance of other kinds of products.

C Doyle - One of the best experts on this subject based on the ideXlab platform.

  • direct morphological comparison of vacuum plasma sprayed and detonation gun sprayed hydroxyapatite coatings for orthopaedic applications
    Biomaterials, 1999
    Co-Authors: H C Gledhill, I G Turner, C Doyle
    Abstract:

    Abstract Hydroxyapatite coatings on titanium substrates were produced using two thermal spray techniques—vacuum plasma spraying and detonation gun spraying. X-ray diffraction was used to compare crystallinity and residual stresses in the coatings. Porosity was measured using optical microscopy in conjunction with an Image Analysis System. Scanning electron microscopy and surface roughness measurements were used to characterise the surface morphologies of the coatings. The vacuum plasma sprayed coatings were found to have a lower residual stress, a higher crystallinity and a higher level of porosity than the detonation gun coatings. It is concluded that consideration needs to be given to the significance of such variations within the clinical context.