The Experts below are selected from a list of 66 Experts worldwide ranked by ideXlab platform
Toyofumi F. Chen-yoshikawa - One of the best experts on this subject based on the ideXlab platform.
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Immune Function monitoring in lung transplantation using adenosine triphosphate production: time trends and relationship to postoperative infection
Surgery Today, 2017Co-Authors: Mamoru Takahashi, Akihiro Ohsumi, Keiji Ohata, Takeshi Kondo, Hideki Motoyama, Kyoko Hijiya, Akihiro Aoyama, Hiroshi Date, Toyofumi F. Chen-yoshikawaAbstract:Purpose The ImmuKnow (IK) assay is a comprehensive Immune Function Test that involves measuring adenosine triphosphate produced by the cluster of differentiation 4+ T lymphocytes in peripheral blood. The aim of this study was to analyze the time trends of IK values and assess the relationship between IK values and infections in lung transplants. Methods We prospectively collected 178 blood samples from 22 deceased-donor lung transplant (DDLT) recipients and 17 living-donor lobar lung transplant (LDLLT) recipients. A surveillance IK assay was performed postoperatively, then after 1 week and 1, 3, 6, and 12 months. Results Time trends of IK values in stable recipients peaked 1 week after DDLT (477 ± 247 ATP ng/ml), and 1 month after LDLLT (433 ± 134 ng/ml), followed by a gradual decline over 1 year. The mean IK values in infections were significantly lower than those in the stable state (119 vs 312 ATP ng/ml, p = 0.0002). Conclusions IK values increased sharply after lung transplantation and then decreased gradually over time in the first year, suggesting a natural history of Immune Function. IK values were also significantly reduced during infections. These results may provide new insights into the utility of Immune monitoring after lung transplantation.
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Immune Function monitoring in lung transplantation using adenosine triphosphate production: time trends and relationship to postoperative infection.
Surgery Today, 2016Co-Authors: Mamoru Takahashi, Akihiro Ohsumi, Keiji Ohata, Takeshi Kondo, Hideki Motoyama, Kyoko Hijiya, Akihiro Aoyama, Hiroshi Date, Toyofumi F. Chen-yoshikawaAbstract:The ImmuKnow (IK) assay is a comprehensive Immune Function Test that involves measuring adenosine triphosphate produced by the cluster of differentiation 4+ T lymphocytes in peripheral blood. The aim of this study was to analyze the time trends of IK values and assess the relationship between IK values and infections in lung transplants. We prospectively collected 178 blood samples from 22 deceased-donor lung transplant (DDLT) recipients and 17 living-donor lobar lung transplant (LDLLT) recipients. A surveillance IK assay was performed postoperatively, then after 1 week and 1, 3, 6, and 12 months. Time trends of IK values in stable recipients peaked 1 week after DDLT (477 ± 247 ATP ng/ml), and 1 month after LDLLT (433 ± 134 ng/ml), followed by a gradual decline over 1 year. The mean IK values in infections were significantly lower than those in the stable state (119 vs 312 ATP ng/ml, p = 0.0002). IK values increased sharply after lung transplantation and then decreased gradually over time in the first year, suggesting a natural history of Immune Function. IK values were also significantly reduced during infections. These results may provide new insights into the utility of Immune monitoring after lung transplantation.
Donald M. Jensen - One of the best experts on this subject based on the ideXlab platform.
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use of Immune Function Test in monitoring immunosuppression in liver transplant recipients
Clinical Transplantation, 2012Co-Authors: Helen S. Te, K. Dasgupta, Rohit Satoskar, Smruti R. Mohanty, Nancy Reau, James Michael Millis, Donald M. JensenAbstract:Immune Function Test (Immuknow TM ) is a measure of cell- mediated immunity based on peripheral CD4 + T cell adenosine triphosphate activity (desired range, 225-525 ng/mL). We evaluated the role of Immune Function Test (IFT) in monitoring and adjustment of immunosuppression in orthotopic liver transplant (OLT) recipients. A total of 289 IFTs were obtained from 171 patients from March 2007 to June 2008. Graft/patient status was classified as stable, serious infection, or malignancy. IFT levels were analyzed with duration of follow-up after OLT, graft/patient status, and the presence of hepatitis C (HCV) infection. The mean age was 54 ± 14 yr, with 62% men. The median follow-up was 65 (2-249) months. Mean IFT levels were significantly lower in patients who were <24 months than in those 24 months post- OLT (220 ± 19.5 vs. 257 ± 11.3 ng/mL, p = 0.03). Clinically stable patients had higher IFT levels than those with serious infection or malignancy (254 ± 11.1 vs. 162.5 ± 23.9, p < 0.001). HCV-infected patients had lower IFT levels than uninfected patients (206.7 ± 15.7 vs. 273 ± 12.0 ng/mL, p < 0.001). Immunosuppression was reduced in 58 patients with IFT levels <225 ng/mL, and 90% maintained stable graft Function after a median follow-up of 22 (1-39) months. IFT may be a useful tool in monitoring and lowering of immunosuppression in long- term OLT recipients.
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Use of Immune Function Test in monitoring immunosuppression in liver transplant recipients.
Clinical Transplantation, 2012Co-Authors: Helen S. Te, K. Dasgupta, Rohit Satoskar, Smruti R. Mohanty, Nancy Reau, James Michael Millis, Donald M. JensenAbstract:Immune Function Test (Immuknow TM ) is a measure of cell- mediated immunity based on peripheral CD4 + T cell adenosine triphosphate activity (desired range, 225-525 ng/mL). We evaluated the role of Immune Function Test (IFT) in monitoring and adjustment of immunosuppression in orthotopic liver transplant (OLT) recipients. A total of 289 IFTs were obtained from 171 patients from March 2007 to June 2008. Graft/patient status was classified as stable, serious infection, or malignancy. IFT levels were analyzed with duration of follow-up after OLT, graft/patient status, and the presence of hepatitis C (HCV) infection. The mean age was 54 ± 14 yr, with 62% men. The median follow-up was 65 (2-249) months. Mean IFT levels were significantly lower in patients who were
Albert D. M. E. Osterhaus - One of the best experts on this subject based on the ideXlab platform.
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Host resistance to rat cytomegalovirus (RCMV) and Immune Function in adult PVG rats fed herring from the contaminated Baltic Sea
Archives of Toxicology, 1996Co-Authors: Peter S. Ross, Henk Van Loveren, Rik L. De Swart, Helen Van Der Vliet, Arja De Klerk, Helga H. Timmerman, Rob Van Binnendijk, Abraham Brouwer, Albert D. M. E. OsterhausAbstract:The immunotoxic potential of many classes of environmental contaminants has been well established in laboratory studies, with much attention being focussed on aryl hydrocarbon ( Ah )-receptor binding polychlorinated biphenyl (PCB), polychlorinated dibenzo- p -dioxin (PCDD), and polychlorinated dibenzofuran (PCDF) congeners. In a semi-field study, we previously showed that harbour seals ( Phoca vitulina ) fed herring from the contaminated Baltic Sea had lower natural killer cell activity, T-lymphocyte Functionality and delayed-type hypersensitivity responses than seals fed herring from the relatively uncontaminated Atlantic Ocean. While ethical and practical constraints preclude in-depth studies in seals, specific reagents and a wider array of Immune Function Tests allow such studies in laboratory rats. We therefore carried out a feeding study in rats aimed at extending our observations of contaminant-induced immunosuppression in harbour seals. The same two herring batches used in the seal study were freeze-dried, supplemented and fed to female adult PVG rats for a period of 4 12 months. Daily contaminant intakes of 2,3,7,8-tetrachlorodibenzo- p -dioxin (TCDD) toxic equivalents (TEQ) were estimated to be 0.3 ng/kg body weight and 1.6 ng/kg in the Atlantic and Baltic groups, respectively. At the end of the feeding experiment, no contaminant-related changes in spleen CD^+ _4/CD^+ _8 cellularity, natural killer cell activity, or mitogen-induced proliferative responses of thymus or spleen cells could be detected. However, total thymocyte numbers and thymus CD^+ _4/CD^+ _8 ratios were reduced in the Baltic group. A novel model was established to assess the specific T-cell response to rat cytomegalovirus (RCMV). When applied to the feeding study, no differences between the Atlantic and Baltic groups in the RCMV-induced proliferative T-lymphocyte responses could be detected, but virus titres in salivary glands of infected rats of the Baltic Sea group were higher. These elevated RCMV titres and changes in thymus cellularity suggest that the dietary exposure to low levels of contaminants may have been immunotoxic at a level which our Immune Function Test could not otherwise detect. While the herring diet per se appeared to have an effect on several Immune Function parameters, lower plasma thyroid hormone levels in the Baltic Sea group of rats confirmed that exposure to the environmental mixture of contaminants led to adverse PHAH-related health effects.
Mamoru Takahashi - One of the best experts on this subject based on the ideXlab platform.
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Immune Function monitoring in lung transplantation using adenosine triphosphate production: time trends and relationship to postoperative infection
Surgery Today, 2017Co-Authors: Mamoru Takahashi, Akihiro Ohsumi, Keiji Ohata, Takeshi Kondo, Hideki Motoyama, Kyoko Hijiya, Akihiro Aoyama, Hiroshi Date, Toyofumi F. Chen-yoshikawaAbstract:Purpose The ImmuKnow (IK) assay is a comprehensive Immune Function Test that involves measuring adenosine triphosphate produced by the cluster of differentiation 4+ T lymphocytes in peripheral blood. The aim of this study was to analyze the time trends of IK values and assess the relationship between IK values and infections in lung transplants. Methods We prospectively collected 178 blood samples from 22 deceased-donor lung transplant (DDLT) recipients and 17 living-donor lobar lung transplant (LDLLT) recipients. A surveillance IK assay was performed postoperatively, then after 1 week and 1, 3, 6, and 12 months. Results Time trends of IK values in stable recipients peaked 1 week after DDLT (477 ± 247 ATP ng/ml), and 1 month after LDLLT (433 ± 134 ng/ml), followed by a gradual decline over 1 year. The mean IK values in infections were significantly lower than those in the stable state (119 vs 312 ATP ng/ml, p = 0.0002). Conclusions IK values increased sharply after lung transplantation and then decreased gradually over time in the first year, suggesting a natural history of Immune Function. IK values were also significantly reduced during infections. These results may provide new insights into the utility of Immune monitoring after lung transplantation.
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Immune Function monitoring in lung transplantation using adenosine triphosphate production: time trends and relationship to postoperative infection.
Surgery Today, 2016Co-Authors: Mamoru Takahashi, Akihiro Ohsumi, Keiji Ohata, Takeshi Kondo, Hideki Motoyama, Kyoko Hijiya, Akihiro Aoyama, Hiroshi Date, Toyofumi F. Chen-yoshikawaAbstract:The ImmuKnow (IK) assay is a comprehensive Immune Function Test that involves measuring adenosine triphosphate produced by the cluster of differentiation 4+ T lymphocytes in peripheral blood. The aim of this study was to analyze the time trends of IK values and assess the relationship between IK values and infections in lung transplants. We prospectively collected 178 blood samples from 22 deceased-donor lung transplant (DDLT) recipients and 17 living-donor lobar lung transplant (LDLLT) recipients. A surveillance IK assay was performed postoperatively, then after 1 week and 1, 3, 6, and 12 months. Time trends of IK values in stable recipients peaked 1 week after DDLT (477 ± 247 ATP ng/ml), and 1 month after LDLLT (433 ± 134 ng/ml), followed by a gradual decline over 1 year. The mean IK values in infections were significantly lower than those in the stable state (119 vs 312 ATP ng/ml, p = 0.0002). IK values increased sharply after lung transplantation and then decreased gradually over time in the first year, suggesting a natural history of Immune Function. IK values were also significantly reduced during infections. These results may provide new insights into the utility of Immune monitoring after lung transplantation.
Yao Wang - One of the best experts on this subject based on the ideXlab platform.
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application of Immune Function Test to evaluate effect of cytokine induced autologous killer cell infusion for elderly patients with hematological malignancies
Journal of Experimental Hematology, 2010Co-Authors: Bo Yang, Xuechun Lu, Yao WangAbstract:: The aim of study was to explore the efficacy of cytokine induced autologous killer (CIK) cell infusion as an Immune therapy for elderly patients with hematological malignancies. Peripheral blood mononuclear cells (PBMNC) were isolated from 20 elderly patients with hematological malignancies, and then augmented by priming with human recombinant interferon gamma (rhIFN-γ) followed by human recombinant interleukin 2 (rhIL-2) and monoclonal antibody (mAb) against CD3. The obtained autologous CIK cells [(2-3)×10(9)] were infused back to individual patients, then followed by subcutaneous injection of IL-2 at single daily dose of 1×10(6) U for 10 consecutive days. The regimen was repeated every 4 weeks and total 136 cycles of CIK cells transfusion were completed. The changes in cellular Immune Function, tumor-related biological parameters, imaging characteristics, the condition of remission, quality of life (QOL) and survival were assessed. The results indicated that 14 patients received 8 cycles of CIK cells infusion, and 4 cycles were completed in 6 patients. No adverse reaction was observed in all patients. The percentages of CD3+, CD3+CD8+ and CD3+CD56+ cells increased significantly (p<0.05), and serum levels of β2-microglobulin and LDH markedly decreased (p<0.05) after autologous CIK cells transfusion. The tumor-related symptoms were relieved, QOL obviously improved (p<0.01) in all patients. Complete remission was seen in 11 patients, and partial remission was observed in 7 patients. It is concluded that the autologous CIK cell infusion can improve immunity in elderly patients of hematological malignancies and displays its effectiveness and safety for elderly patients.
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Application of Immune Function Test to evaluate effect of cytokine-induced autologous killer cell infusion for elderly patients with hematological malignancies
Journal of Experimental Hematology, 2010Co-Authors: Bo Yang, Xuechun Lu, Yao WangAbstract:: The aim of study was to explore the efficacy of cytokine induced autologous killer (CIK) cell infusion as an Immune therapy for elderly patients with hematological malignancies. Peripheral blood mononuclear cells (PBMNC) were isolated from 20 elderly patients with hematological malignancies, and then augmented by priming with human recombinant interferon gamma (rhIFN-γ) followed by human recombinant interleukin 2 (rhIL-2) and monoclonal antibody (mAb) against CD3. The obtained autologous CIK cells [(2-3)×10(9)] were infused back to individual patients, then followed by subcutaneous injection of IL-2 at single daily dose of 1×10(6) U for 10 consecutive days. The regimen was repeated every 4 weeks and total 136 cycles of CIK cells transfusion were completed. The changes in cellular Immune Function, tumor-related biological parameters, imaging characteristics, the condition of remission, quality of life (QOL) and survival were assessed. The results indicated that 14 patients received 8 cycles of CIK cells infusion, and 4 cycles were completed in 6 patients. No adverse reaction was observed in all patients. The percentages of CD3+, CD3+CD8+ and CD3+CD56+ cells increased significantly (p
