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Xie Shouqi - One of the best experts on this subject based on the ideXlab platform.
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dietary supplementation of geotrichum candidum improves growth gut microbiota immune related Gene expression and disease resistance in gibel carp cas iii carassius auratus gibelio
Fish & Shellfish Immunology, 2020Co-Authors: Huda Noorul, Liu Haokun, Jin Junyan, Zhu Xiaoming, Han Dong, Yang Yunxia, Xie ShouqiAbstract:Abstract Geotrichum candidum is a filamentous fungus mostly used in the dairy industry for cheese ripening and flavoring. The current study was designed to evaluate the potential probiotics effect of dietary supplementation of G. candidum on growth, digestive enzymes activities, gut microbiota and Immune-Related Gene expression and disease resistance in Gibel carp CAS Ⅲ (Carassius auratus gibelio) against A. hydrophila infections. For this purpose, 420 healthy fish (initial body weight: 7.09 ± 0.02g) were randomly divided into 7 feeding groups in triplicates of 20 fish in each tank. Seven isocaloric and isonitrogenous diets were prepared, supplemented with 6 different doses of G. candidum viz; C: 0, T1:106, T2:107, T3:108, T4:109, T5:1010, T6:1011 CFU/kg diet and fed to fish twice a day for 60 days. Results showed significantly higher weight gain (WG) and feed efficiency (FE) in groups fed 106 and 108 CFU/kg diet compared to the control (P All G. candidum fed groups showed higher intestinal α-amylase activity (P After challenge test immune response expressed numerically higher survival rate (P > 0.05) and significantly higher (P 0.05) lysozyme activity, and Alanine Transaminase activity (ALT) in groups fed 106 and 108 CFU/kg G. candidum supplemented diet. The expression of immune related Gene (IL-I β, TNF- α, HSP70 and TLR-2) in liver of the fish were significantly affected (P
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Dietary supplementation of Geotrichum candidum improves growth, gut microbiota, Immune-Related Gene expression and disease resistance in gibel carp CAS III (Carassius auratus gibelio)
'Elsevier BV', 2020Co-Authors: Noor-ul Huda, Liu Haokun, Jin Junyan, Zhu Xiaoming, Han Dong, Yang Yunxia, Xie ShouqiAbstract:Geotrichum candidum is a filamentous fungus mostly used in the dairy industry for cheese ripening and flavoring. The current study was designed to evaluate the potential probiotics effect of dietary supplementation of G. candidum on growth, digestive enzymes activities, gut microbiota and Immune-Related Gene expression and disease resistance in Gibel carp CAS III (Carassius auratus gibelio) against A. hydrophila infections. For this purpose, 420 healthy fish (initial body weight: 7.09 +/- 0.02g) were randomly divided into 7 feeding groups in triplicates of 20 fish in each tank. Seven isocaloric and isonitrogenous diets were prepared, supplemented with 6 different doses of G. candidum viz; C: 0, T1:10(6), T2:10(7), T3:10(8), T4:10(9), T5:10(10), T6:10(11) CFU/kg diet and fed to fish twice a day for 60 days. Results showed significantly higher weight gain (WG) and feed efficiency (FE) in groups fed 10(6) and 10(8) CFU/kg diet compared to the control (P < 0.05). All G. candidum fed groups showed higher intestinal a-amylase activity (P < 0.05) while no difference in lipase and trypsin activity compared to the control group. Before challenge test, gut microbial diversity analysis revealed relatively more abundance of bacteria belonging to phylum Proteobacteria, Actinobacteria, Firmicutes and Bacteriodetes in group fed G. candidum supplemented diet and higher percentage of pathogenic bacteria belonging to order Aeromonadales and Vibrionales in control post-challenge. After challenge test immune response expressed numerically higher survival rate (P > 0.05) and significantly higher (P 0.05) lysozyme activity, and Alanine Transaminase activity (ALT) in groups fed 10(6) and 10(8) CFU/kg G. candidum supplemented diet. The expression of immune related Gene (IL-I beta, TNF- alpha, HSP70 and TLR-2) in liver of the fish were significantly affected (P < 0.05) by supplementation of G. candidum post bacterial challenge. The results showed that G. candidum is a potential probiotic as it could improve feed utilization, immunity and cause no harmful effects on growth of gibel carp at higher supplementation levels. The productive results can be achieved by using the lower dose as 10(6)-10(8) CFU/kg diet
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Dietary supplementation of Geotrichum candidum improves growth, gut microbiota, Immune-Related Gene expression and disease resistance in gibel carp CAS III (Carassius auratus gibelio)
'Elsevier BV', 2020Co-Authors: Noor-ul Huda, Liu Haokun, Jin Junyan, Zhu Xiaoming, Han Dong, Yang Yunxia, Xie ShouqiAbstract:Geotrichum candidum is a filamentous fungus mostly used in the dairy industry for cheese ripening and flavoring. The current study was designed to evaluate the potential probiotics effect of dietary supplementation of G. candidum on growth, digestive enzymes activities, gut microbiota and Immune-Related Gene expression and disease resistance in Gibel carp CAS III (Carassius auratus gibelio) against A. hydrophila infections. For this purpose, 420 healthy fish (initial body weight: 7.09 +/- 0.02g) were randomly divided into 7 feeding groups in triplicates of 20 fish in each tank. Seven isocaloric and isonitrogenous diets were prepared, supplemented with 6 different doses of G. candidum viz; C: 0, T1:10(6), T2:10(7), T3:10(8), T4:10(9), T5:10(10), T6:10(11) CFU/kg diet and fed to fish twice a day for 60 days. Results showed significantly higher weight gain (WG) and feed efficiency (FE) in groups fed 10(6) and 10(8) CFU/kg diet compared to the control (P < 0.05). All G. candidum fed groups showed higher intestinal a-amylase activity (P < 0.05) while no difference in lipase and trypsin activity compared to the control group. Before challenge test, gut microbial diversity analysis revealed relatively more abundance of bacteria belonging to phylum Proteobacteria, Actinobacteria, Firmicutes and Bacteriodetes in group fed G. candidum supplemented diet and higher percentage of pathogenic bacteria belonging to order Aeromonadales and Vibrionales in control post-challenge. After challenge test immune response expressed numerically higher survival rate (P > 0.05) and significantly higher (P < 0.05) Respiratory Burst Activity(RBA), Immunoglobulin M level (IgM), Aspartate Transaminase activity (AST) and improved (P > 0.05) lysozyme activity, and Alanine Transaminase activity (ALT) in groups fed 10(6) and 10(8) CFU/kg G. candidum supplemented diet. The expression of immune related Gene (IL-I beta, TNF- alpha, HSP70 and TLR-2) in liver of the fish were significantly affected (P < 0.05) by supplementation of G. candidum post bacterial challenge. The results showed that G. candidum is a potential probiotic as it could improve feed utilization, immunity and cause no harmful effects on growth of gibel carp at higher supplementation levels. The productive results can be achieved by using the lower dose as 10(6)-10(8) CFU/kg diet.
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Effects of dietary Arthrospira platensis supplementation on the growth performance, antioxidation and immune related -Gene expression in yellow catfish (Pelteobagrus fulvidraco)
'Elsevier BV', 2020Co-Authors: Liu Cui, Liu Haokun, Jin Junyan, Han Dong, Yang Yunxia, Xie Shouqi, Zhu XiaomingAbstract:This study evaluated the effects of five isonitrogenous (405 g crude protein kg(-1) diet) and isolipidic (85 g crude lipid kg(-1) diet) diets containing 0, 5, 10, 20 and 40 g kg(-1) Arthrospira platensis on the growth performance, antioxidation and immune related-Gene expression of yellow catfish (Pelteobagrus fulvidraco ). A completely randomized experimental design was developed with five treatments and three replicates. Apparent healthy and similar size fish (n = 225, 20.87 +/- 0.06 g) were randomly selected, batch weighed and stocked into fifteen 150 L fiber glass tanks at a density of 15 fish per tank. The experiment lasted for 56 days. After the growth trial, bacterial challenge test was conducted. The results showed that no obvious difference in feed intake and growth performance were found among groups (P 0.05). After A. hydrophila challenge, supplementing 40 g kg(-1) dietary A. platensis could obviously increase the level of plasma IgM, C3 and GPX (P < 0.05). A. platensis supplemented diet could decrease the level of plasma MDA and increase the activity of plasma lysozyme (LZM) (P < 0.05). Transcriptional levels of SOD , CAT , GPX , il-1 beta, il-8 , il-10 and lys Genes in spleen and kidney after challenge were significantly up-regulated in 40 g kg(-1) dietary A. platensis group compared with the control group catfish (P < 0.05). Taken together, A. platensis can be introduced as a functional additive in yellow catfish diets. Inclusion of 40 g kg(-1) dietary A. platensis was found to be a suitable level to enhance the ability of antioxidant and immunity as well as no negative effects on fish growth
Angeles M Esteban - One of the best experts on this subject based on the ideXlab platform.
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enrichment of gilthead seabream sparus aurata l diet with palm fruit extracts and probiotics effects on skin mucosal immunity
Fish & Shellfish Immunology, 2016Co-Authors: Rebeca Cerezuela, Francisco A Guardiola, Alberto Cuesta, Angeles M EstebanAbstract:Fish skin mucus contains numerous immune substances still poorly studied. To date, there are no studies regarding the possible influence of dietary supplements on such important substances. In the present work, a commercial diet used as control diet was enriched with: 1) probiotic Shewanella putrefaciens (Pdp11 diet, 10(9) cfu g(-1)); 2) probiotic Bacillus sp. (Bacillus diet, 10(9) ufc g(-1)); 3) aqueous date palm fruits extracts (DPE diet, 4%), and 4) a combination of Pdp11 + Bacillus sp + aqueous DPE (Mix diet). After 2 and 4 weeks of the feeding trial, enzymatic activities (proteases, antiproteases and peroxidases), IgM levels and terminal carbohydrates abundance were determined in skin mucus. In addition, the expression of certain immune related Genes was evaluated in the skin. Our results demonstrated the significant alteration of the terminal carbohydrate abundance in skin mucus. Carbohydrates more affected by experimental diets were N-acetyl-galactosamine, N-acetyl-glucosamine, galactose, mannose, glucose and fucose. IgM, peroxidase activity and protease were also significantly higher in fish fed enriched diets. For last, an important up-regulation on the immune related Gene studied on the skin was also detected. Present findings provide robust evidence that fish skin mucosal immunity can be improved by the diet.
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effects of date palm fruit extracts on skin mucosal immunity immune related Genes expression and growth performance of common carp cyprinus carpio fry
Fish & Shellfish Immunology, 2015Co-Authors: Seyed Hossein Hoseinifar, Mohsen Khalili, Rudabeh Rufchaei, Mojtaba Raeisi, Marzieh Attar, Hector Cordero, Angeles M EstebanAbstract:The aim of this study was to investigate the effects of date palm fruit extracts (DPFE) on skin mucosal immunity, immune related Genes expression and growth performance of fry common carp (Cyprinus carpio). One hundred and twenty specimens (4.06 ± 0.13 g) were supplied and allocated into six aquaria; specimens in three aquaria were fed non-supplemented diet (control) while the fish in the other 3 aquaria were fed with DPFE at 200 ml kg(-1). At the end of feeding trial (8 weeks) skin mucus immune parameters (total immunoglobulins, lysozyme, protease and alkaline phosphatase activity) and immune related Gene expression (tumor necrosis factor α [tnfa], lysozyme [ly] and interleukin-1-beta, [il1b]) in the head-kidney were studied. The results revealed that feeding carp fry with 200 ml kg(-1) DPFE remarkably elevated the three skin mucus immune parameters tested (P 0.05) compared to control fish (fed control diet). Furthermore, growth performance parameters were significantly improved in fry fed DPFE (P < 0.05). More studies are needed to understand different aspects of DPFE administration in fry mucosal immunity.
Heiko Vogel - One of the best experts on this subject based on the ideXlab platform.
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bacterial feeding induces changes in immune related Gene expression and has trans Generational impacts in the cabbage looper trichoplusia ni
Frontiers in Zoology, 2009Co-Authors: Dalial Freitak, David G Heckel, Heiko VogelAbstract:Background Poly- and oligophagous insects are able to feed on various host plants with a wide range of defense strategies. However, diverse food plants are also inhabited by microbiota differing in quality and quantity, posing a potential challenge for immune system mediated homeostasis in the herbivore. Recent studies highlight the complex interactions between environmentally encountered microorganisms and herbivorous insects, pointing to a potential adaptational alteration of the insects' physiology. We performed a differential Gene expression analysis in whole larvae and eggs laid by parents grown on different diets to identify potential novel Genes related to elevated microbial content in the caterpillars' food.
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bacterial feeding induces changes in immune related Gene expression and has trans Generational impacts in the cabbage looper trichoplusia ni
Frontiers in Zoology, 2009Co-Authors: Dalial Freitak, David G Heckel, Heiko VogelAbstract:Poly- and oligophagous insects are able to feed on various host plants with a wide range of defense strategies. However, diverse food plants are also inhabited by microbiota differing in quality and quantity, posing a potential challenge for immune system mediated homeostasis in the herbivore. Recent studies highlight the complex interactions between environmentally encountered microorganisms and herbivorous insects, pointing to a potential adaptational alteration of the insects' physiology. We performed a differential Gene expression analysis in whole larvae and eggs laid by parents grown on different diets to identify potential novel Genes related to elevated microbial content in the caterpillars' food. We used GeneFishing, a novel differential display method, to study the effects of dietary bacteria on the General Gene expression in different life stages and tissues of the cabbage looper (Trichoplusia ni). We were able to visualize several hundred transcripts on agarose gels, one fifth of which were differentially expressed between treatments. The largest number of differentially expressed Genes was found in defense-related processes (13) and in recognition and metabolism (16). 21 Genes were picked out and further tested for differential Gene expression by an independent method (qRT-PCR) in various tissues of larvae grown on bacterial and bacteria-free diet, and also in adults. We detected a number of Genes indicative of an altered physiological status of the insect, depending on the diet, developmental stage and tissue. Changes in immune status are accompanied by specific changes in the transcript levels of Genes connected to metabolism and homeostasis of the organism. Our findings show that larval feeding on bacteria-rich diet leads to substantial Gene expression changes, potentially resulting in a reorganization of the insects' metabolism to maintain organismal homeostasis, not only in the larval but also in the adult stage. Furthermore, differences in Gene expression levels can also be seen in the next Generation, strongly influenced by parental diet.
Zhu Xiaoming - One of the best experts on this subject based on the ideXlab platform.
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dietary supplementation of geotrichum candidum improves growth gut microbiota immune related Gene expression and disease resistance in gibel carp cas iii carassius auratus gibelio
Fish & Shellfish Immunology, 2020Co-Authors: Huda Noorul, Liu Haokun, Jin Junyan, Zhu Xiaoming, Han Dong, Yang Yunxia, Xie ShouqiAbstract:Abstract Geotrichum candidum is a filamentous fungus mostly used in the dairy industry for cheese ripening and flavoring. The current study was designed to evaluate the potential probiotics effect of dietary supplementation of G. candidum on growth, digestive enzymes activities, gut microbiota and Immune-Related Gene expression and disease resistance in Gibel carp CAS Ⅲ (Carassius auratus gibelio) against A. hydrophila infections. For this purpose, 420 healthy fish (initial body weight: 7.09 ± 0.02g) were randomly divided into 7 feeding groups in triplicates of 20 fish in each tank. Seven isocaloric and isonitrogenous diets were prepared, supplemented with 6 different doses of G. candidum viz; C: 0, T1:106, T2:107, T3:108, T4:109, T5:1010, T6:1011 CFU/kg diet and fed to fish twice a day for 60 days. Results showed significantly higher weight gain (WG) and feed efficiency (FE) in groups fed 106 and 108 CFU/kg diet compared to the control (P All G. candidum fed groups showed higher intestinal α-amylase activity (P After challenge test immune response expressed numerically higher survival rate (P > 0.05) and significantly higher (P 0.05) lysozyme activity, and Alanine Transaminase activity (ALT) in groups fed 106 and 108 CFU/kg G. candidum supplemented diet. The expression of immune related Gene (IL-I β, TNF- α, HSP70 and TLR-2) in liver of the fish were significantly affected (P
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Dietary supplementation of Geotrichum candidum improves growth, gut microbiota, Immune-Related Gene expression and disease resistance in gibel carp CAS III (Carassius auratus gibelio)
'Elsevier BV', 2020Co-Authors: Noor-ul Huda, Liu Haokun, Jin Junyan, Zhu Xiaoming, Han Dong, Yang Yunxia, Xie ShouqiAbstract:Geotrichum candidum is a filamentous fungus mostly used in the dairy industry for cheese ripening and flavoring. The current study was designed to evaluate the potential probiotics effect of dietary supplementation of G. candidum on growth, digestive enzymes activities, gut microbiota and Immune-Related Gene expression and disease resistance in Gibel carp CAS III (Carassius auratus gibelio) against A. hydrophila infections. For this purpose, 420 healthy fish (initial body weight: 7.09 +/- 0.02g) were randomly divided into 7 feeding groups in triplicates of 20 fish in each tank. Seven isocaloric and isonitrogenous diets were prepared, supplemented with 6 different doses of G. candidum viz; C: 0, T1:10(6), T2:10(7), T3:10(8), T4:10(9), T5:10(10), T6:10(11) CFU/kg diet and fed to fish twice a day for 60 days. Results showed significantly higher weight gain (WG) and feed efficiency (FE) in groups fed 10(6) and 10(8) CFU/kg diet compared to the control (P < 0.05). All G. candidum fed groups showed higher intestinal a-amylase activity (P < 0.05) while no difference in lipase and trypsin activity compared to the control group. Before challenge test, gut microbial diversity analysis revealed relatively more abundance of bacteria belonging to phylum Proteobacteria, Actinobacteria, Firmicutes and Bacteriodetes in group fed G. candidum supplemented diet and higher percentage of pathogenic bacteria belonging to order Aeromonadales and Vibrionales in control post-challenge. After challenge test immune response expressed numerically higher survival rate (P > 0.05) and significantly higher (P 0.05) lysozyme activity, and Alanine Transaminase activity (ALT) in groups fed 10(6) and 10(8) CFU/kg G. candidum supplemented diet. The expression of immune related Gene (IL-I beta, TNF- alpha, HSP70 and TLR-2) in liver of the fish were significantly affected (P < 0.05) by supplementation of G. candidum post bacterial challenge. The results showed that G. candidum is a potential probiotic as it could improve feed utilization, immunity and cause no harmful effects on growth of gibel carp at higher supplementation levels. The productive results can be achieved by using the lower dose as 10(6)-10(8) CFU/kg diet
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Dietary supplementation of Geotrichum candidum improves growth, gut microbiota, Immune-Related Gene expression and disease resistance in gibel carp CAS III (Carassius auratus gibelio)
'Elsevier BV', 2020Co-Authors: Noor-ul Huda, Liu Haokun, Jin Junyan, Zhu Xiaoming, Han Dong, Yang Yunxia, Xie ShouqiAbstract:Geotrichum candidum is a filamentous fungus mostly used in the dairy industry for cheese ripening and flavoring. The current study was designed to evaluate the potential probiotics effect of dietary supplementation of G. candidum on growth, digestive enzymes activities, gut microbiota and Immune-Related Gene expression and disease resistance in Gibel carp CAS III (Carassius auratus gibelio) against A. hydrophila infections. For this purpose, 420 healthy fish (initial body weight: 7.09 +/- 0.02g) were randomly divided into 7 feeding groups in triplicates of 20 fish in each tank. Seven isocaloric and isonitrogenous diets were prepared, supplemented with 6 different doses of G. candidum viz; C: 0, T1:10(6), T2:10(7), T3:10(8), T4:10(9), T5:10(10), T6:10(11) CFU/kg diet and fed to fish twice a day for 60 days. Results showed significantly higher weight gain (WG) and feed efficiency (FE) in groups fed 10(6) and 10(8) CFU/kg diet compared to the control (P < 0.05). All G. candidum fed groups showed higher intestinal a-amylase activity (P < 0.05) while no difference in lipase and trypsin activity compared to the control group. Before challenge test, gut microbial diversity analysis revealed relatively more abundance of bacteria belonging to phylum Proteobacteria, Actinobacteria, Firmicutes and Bacteriodetes in group fed G. candidum supplemented diet and higher percentage of pathogenic bacteria belonging to order Aeromonadales and Vibrionales in control post-challenge. After challenge test immune response expressed numerically higher survival rate (P > 0.05) and significantly higher (P < 0.05) Respiratory Burst Activity(RBA), Immunoglobulin M level (IgM), Aspartate Transaminase activity (AST) and improved (P > 0.05) lysozyme activity, and Alanine Transaminase activity (ALT) in groups fed 10(6) and 10(8) CFU/kg G. candidum supplemented diet. The expression of immune related Gene (IL-I beta, TNF- alpha, HSP70 and TLR-2) in liver of the fish were significantly affected (P < 0.05) by supplementation of G. candidum post bacterial challenge. The results showed that G. candidum is a potential probiotic as it could improve feed utilization, immunity and cause no harmful effects on growth of gibel carp at higher supplementation levels. The productive results can be achieved by using the lower dose as 10(6)-10(8) CFU/kg diet.
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Effects of dietary Arthrospira platensis supplementation on the growth performance, antioxidation and immune related -Gene expression in yellow catfish (Pelteobagrus fulvidraco)
'Elsevier BV', 2020Co-Authors: Liu Cui, Liu Haokun, Jin Junyan, Han Dong, Yang Yunxia, Xie Shouqi, Zhu XiaomingAbstract:This study evaluated the effects of five isonitrogenous (405 g crude protein kg(-1) diet) and isolipidic (85 g crude lipid kg(-1) diet) diets containing 0, 5, 10, 20 and 40 g kg(-1) Arthrospira platensis on the growth performance, antioxidation and immune related-Gene expression of yellow catfish (Pelteobagrus fulvidraco ). A completely randomized experimental design was developed with five treatments and three replicates. Apparent healthy and similar size fish (n = 225, 20.87 +/- 0.06 g) were randomly selected, batch weighed and stocked into fifteen 150 L fiber glass tanks at a density of 15 fish per tank. The experiment lasted for 56 days. After the growth trial, bacterial challenge test was conducted. The results showed that no obvious difference in feed intake and growth performance were found among groups (P 0.05). After A. hydrophila challenge, supplementing 40 g kg(-1) dietary A. platensis could obviously increase the level of plasma IgM, C3 and GPX (P < 0.05). A. platensis supplemented diet could decrease the level of plasma MDA and increase the activity of plasma lysozyme (LZM) (P < 0.05). Transcriptional levels of SOD , CAT , GPX , il-1 beta, il-8 , il-10 and lys Genes in spleen and kidney after challenge were significantly up-regulated in 40 g kg(-1) dietary A. platensis group compared with the control group catfish (P < 0.05). Taken together, A. platensis can be introduced as a functional additive in yellow catfish diets. Inclusion of 40 g kg(-1) dietary A. platensis was found to be a suitable level to enhance the ability of antioxidant and immunity as well as no negative effects on fish growth
Liu Haokun - One of the best experts on this subject based on the ideXlab platform.
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dietary supplementation of geotrichum candidum improves growth gut microbiota immune related Gene expression and disease resistance in gibel carp cas iii carassius auratus gibelio
Fish & Shellfish Immunology, 2020Co-Authors: Huda Noorul, Liu Haokun, Jin Junyan, Zhu Xiaoming, Han Dong, Yang Yunxia, Xie ShouqiAbstract:Abstract Geotrichum candidum is a filamentous fungus mostly used in the dairy industry for cheese ripening and flavoring. The current study was designed to evaluate the potential probiotics effect of dietary supplementation of G. candidum on growth, digestive enzymes activities, gut microbiota and Immune-Related Gene expression and disease resistance in Gibel carp CAS Ⅲ (Carassius auratus gibelio) against A. hydrophila infections. For this purpose, 420 healthy fish (initial body weight: 7.09 ± 0.02g) were randomly divided into 7 feeding groups in triplicates of 20 fish in each tank. Seven isocaloric and isonitrogenous diets were prepared, supplemented with 6 different doses of G. candidum viz; C: 0, T1:106, T2:107, T3:108, T4:109, T5:1010, T6:1011 CFU/kg diet and fed to fish twice a day for 60 days. Results showed significantly higher weight gain (WG) and feed efficiency (FE) in groups fed 106 and 108 CFU/kg diet compared to the control (P All G. candidum fed groups showed higher intestinal α-amylase activity (P After challenge test immune response expressed numerically higher survival rate (P > 0.05) and significantly higher (P 0.05) lysozyme activity, and Alanine Transaminase activity (ALT) in groups fed 106 and 108 CFU/kg G. candidum supplemented diet. The expression of immune related Gene (IL-I β, TNF- α, HSP70 and TLR-2) in liver of the fish were significantly affected (P
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Dietary supplementation of Geotrichum candidum improves growth, gut microbiota, Immune-Related Gene expression and disease resistance in gibel carp CAS III (Carassius auratus gibelio)
'Elsevier BV', 2020Co-Authors: Noor-ul Huda, Liu Haokun, Jin Junyan, Zhu Xiaoming, Han Dong, Yang Yunxia, Xie ShouqiAbstract:Geotrichum candidum is a filamentous fungus mostly used in the dairy industry for cheese ripening and flavoring. The current study was designed to evaluate the potential probiotics effect of dietary supplementation of G. candidum on growth, digestive enzymes activities, gut microbiota and Immune-Related Gene expression and disease resistance in Gibel carp CAS III (Carassius auratus gibelio) against A. hydrophila infections. For this purpose, 420 healthy fish (initial body weight: 7.09 +/- 0.02g) were randomly divided into 7 feeding groups in triplicates of 20 fish in each tank. Seven isocaloric and isonitrogenous diets were prepared, supplemented with 6 different doses of G. candidum viz; C: 0, T1:10(6), T2:10(7), T3:10(8), T4:10(9), T5:10(10), T6:10(11) CFU/kg diet and fed to fish twice a day for 60 days. Results showed significantly higher weight gain (WG) and feed efficiency (FE) in groups fed 10(6) and 10(8) CFU/kg diet compared to the control (P < 0.05). All G. candidum fed groups showed higher intestinal a-amylase activity (P < 0.05) while no difference in lipase and trypsin activity compared to the control group. Before challenge test, gut microbial diversity analysis revealed relatively more abundance of bacteria belonging to phylum Proteobacteria, Actinobacteria, Firmicutes and Bacteriodetes in group fed G. candidum supplemented diet and higher percentage of pathogenic bacteria belonging to order Aeromonadales and Vibrionales in control post-challenge. After challenge test immune response expressed numerically higher survival rate (P > 0.05) and significantly higher (P 0.05) lysozyme activity, and Alanine Transaminase activity (ALT) in groups fed 10(6) and 10(8) CFU/kg G. candidum supplemented diet. The expression of immune related Gene (IL-I beta, TNF- alpha, HSP70 and TLR-2) in liver of the fish were significantly affected (P < 0.05) by supplementation of G. candidum post bacterial challenge. The results showed that G. candidum is a potential probiotic as it could improve feed utilization, immunity and cause no harmful effects on growth of gibel carp at higher supplementation levels. The productive results can be achieved by using the lower dose as 10(6)-10(8) CFU/kg diet
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Dietary supplementation of Geotrichum candidum improves growth, gut microbiota, Immune-Related Gene expression and disease resistance in gibel carp CAS III (Carassius auratus gibelio)
'Elsevier BV', 2020Co-Authors: Noor-ul Huda, Liu Haokun, Jin Junyan, Zhu Xiaoming, Han Dong, Yang Yunxia, Xie ShouqiAbstract:Geotrichum candidum is a filamentous fungus mostly used in the dairy industry for cheese ripening and flavoring. The current study was designed to evaluate the potential probiotics effect of dietary supplementation of G. candidum on growth, digestive enzymes activities, gut microbiota and Immune-Related Gene expression and disease resistance in Gibel carp CAS III (Carassius auratus gibelio) against A. hydrophila infections. For this purpose, 420 healthy fish (initial body weight: 7.09 +/- 0.02g) were randomly divided into 7 feeding groups in triplicates of 20 fish in each tank. Seven isocaloric and isonitrogenous diets were prepared, supplemented with 6 different doses of G. candidum viz; C: 0, T1:10(6), T2:10(7), T3:10(8), T4:10(9), T5:10(10), T6:10(11) CFU/kg diet and fed to fish twice a day for 60 days. Results showed significantly higher weight gain (WG) and feed efficiency (FE) in groups fed 10(6) and 10(8) CFU/kg diet compared to the control (P < 0.05). All G. candidum fed groups showed higher intestinal a-amylase activity (P < 0.05) while no difference in lipase and trypsin activity compared to the control group. Before challenge test, gut microbial diversity analysis revealed relatively more abundance of bacteria belonging to phylum Proteobacteria, Actinobacteria, Firmicutes and Bacteriodetes in group fed G. candidum supplemented diet and higher percentage of pathogenic bacteria belonging to order Aeromonadales and Vibrionales in control post-challenge. After challenge test immune response expressed numerically higher survival rate (P > 0.05) and significantly higher (P < 0.05) Respiratory Burst Activity(RBA), Immunoglobulin M level (IgM), Aspartate Transaminase activity (AST) and improved (P > 0.05) lysozyme activity, and Alanine Transaminase activity (ALT) in groups fed 10(6) and 10(8) CFU/kg G. candidum supplemented diet. The expression of immune related Gene (IL-I beta, TNF- alpha, HSP70 and TLR-2) in liver of the fish were significantly affected (P < 0.05) by supplementation of G. candidum post bacterial challenge. The results showed that G. candidum is a potential probiotic as it could improve feed utilization, immunity and cause no harmful effects on growth of gibel carp at higher supplementation levels. The productive results can be achieved by using the lower dose as 10(6)-10(8) CFU/kg diet.
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Effects of dietary Arthrospira platensis supplementation on the growth performance, antioxidation and immune related -Gene expression in yellow catfish (Pelteobagrus fulvidraco)
'Elsevier BV', 2020Co-Authors: Liu Cui, Liu Haokun, Jin Junyan, Han Dong, Yang Yunxia, Xie Shouqi, Zhu XiaomingAbstract:This study evaluated the effects of five isonitrogenous (405 g crude protein kg(-1) diet) and isolipidic (85 g crude lipid kg(-1) diet) diets containing 0, 5, 10, 20 and 40 g kg(-1) Arthrospira platensis on the growth performance, antioxidation and immune related-Gene expression of yellow catfish (Pelteobagrus fulvidraco ). A completely randomized experimental design was developed with five treatments and three replicates. Apparent healthy and similar size fish (n = 225, 20.87 +/- 0.06 g) were randomly selected, batch weighed and stocked into fifteen 150 L fiber glass tanks at a density of 15 fish per tank. The experiment lasted for 56 days. After the growth trial, bacterial challenge test was conducted. The results showed that no obvious difference in feed intake and growth performance were found among groups (P 0.05). After A. hydrophila challenge, supplementing 40 g kg(-1) dietary A. platensis could obviously increase the level of plasma IgM, C3 and GPX (P < 0.05). A. platensis supplemented diet could decrease the level of plasma MDA and increase the activity of plasma lysozyme (LZM) (P < 0.05). Transcriptional levels of SOD , CAT , GPX , il-1 beta, il-8 , il-10 and lys Genes in spleen and kidney after challenge were significantly up-regulated in 40 g kg(-1) dietary A. platensis group compared with the control group catfish (P < 0.05). Taken together, A. platensis can be introduced as a functional additive in yellow catfish diets. Inclusion of 40 g kg(-1) dietary A. platensis was found to be a suitable level to enhance the ability of antioxidant and immunity as well as no negative effects on fish growth
