The Experts below are selected from a list of 5142 Experts worldwide ranked by ideXlab platform
Eiichi Uchio - One of the best experts on this subject based on the ideXlab platform.
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evaluation of a silver amplified Immunochromatography kit for adenoviral conjunctivitis
Journal of Medical Virology, 2019Co-Authors: Tsuguto Fujimoto, Tomoko Tsukaharakawamura, Nozomu Hanaoka, Eiichi Uchio, Masami Konagaya, Masaaki Kobayashi, Hisashi Nakagawa, Hiroshi Hatano, Hisatoshi KanekoAbstract:OBJECTIVE To compare and evaluate the sensitivity of a newly developed silver-amplified Immunochromatography (SAI) kit with various Immunochromatography (IC) kits for adenoviruses based on the detection limit (copies/test). METHODS An SAI kit and four ophthalmic IC kits were evaluated. The detection limits of the five kits were determined using the limiting dilution method for 15 conjunctivitis-associated adenoviruses (adenoviruses 1, 2, 3, 4, 5, 7, 8, 11, 37, 53, 54, 56, 64, 81, and 85). The detection limits were presented as numerical values as determined by real-time polymerase chain reaction (PCR). RESULTS The detection limit of the SAI kit for the adenovirus types ranged from 1.0 × 103 -5.0 × 10 4 copies/test (geometric mean, 4.7 × 10 3 ). SAI had a 10-250-fold lower detection limit than the four IC kits for all adenoviruses studied. There were also differences in detection limits among the adenovirus types for each kit. DISCUSSION The detection limit of the SAI kit was drastically reduced because the silver-amplification reaction increased the color development sensitivity. The results revealed the high sensitivity of SAI for detecting adenoviruses and suggested its usefulness for conjunctivitis examination.
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Evaluation of adenovirus amplified detection of immunochromatographic test using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis
Graefe's Archive for Clinical and Experimental Ophthalmology, 2019Co-Authors: Hironori Migita, Tomohiro Ueno, Yusuke Saeki, Nozomu Hanaoka, Tsuguto Fujimoto, Tomoko Tsukahara-kawamura, Eiichi UchioAbstract:Background We evaluated a novel silver amplification Immunochromatography test for rapid detection of adenovirus (AdV) antigen equipped with an automated reader system using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis. Methods Two kinds of immunochromatographic (IC) kits, a conventional IC kit for conjunctival scrapings (control kit) and an IC kit using tears including conjunctival exudate collected by pressing a filter paper strip on the conjunctiva (test kit), were tested on 90 patients who attended Migita Eye Clinic with suspected adenoviral conjunctivitis. The results of the test kits were automatically obtained by a specific reader, which was based on silver amplification Immunochromatography system, in 15 min. The detection of AdV was confirmed by real-time polymerase chain reaction (PCR) method, and typing was performed by direct sequencing. Comparative dilution assay was carried out with the two kits, using AdV type 3 and type 54 strains. Results The sensitivity of the control kit and test kit was 89.8% and 98.3%, respectively. The specificity of both kits was 100%. A significant difference in the sensitivities of the two IC kits against PCR positivity was observed ( P
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evaluation of adenovirus amplified detection of immunochromatographic test using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis
Graefes Archive for Clinical and Experimental Ophthalmology, 2019Co-Authors: Hironori Migita, Tomohiro Ueno, Tomoko Tsukaharakawamura, Yusuke Saeki, Nozomu Hanaoka, Tsuguto Fujimoto, Eiichi UchioAbstract:Background We evaluated a novel silver amplification Immunochromatography test for rapid detection of adenovirus (AdV) antigen equipped with an automated reader system using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis.
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Evaluation of a silver‐amplified Immunochromatography kit for adenoviral conjunctivitis
Journal of medical virology, 2019Co-Authors: Tsuguto Fujimoto, Nozomu Hanaoka, Eiichi Uchio, Tomoko Tsukahara-kawamura, Masami Konagaya, Masaaki Kobayashi, Hisashi Nakagawa, Hiroshi Hatano, Hisatoshi KanekoAbstract:Objective To compare and evaluate the sensitivity of a newly developed silver-amplified Immunochromatography (SAI) kit with various Immunochromatography (IC) kits for adenoviruses based on the detection limit (copies/test). Methods An SAI kit and four ophthalmic IC kits were evaluated. The detection limits of the five kits were determined using the limiting dilution method for 15 conjunctivitis-associated adenoviruses (adenoviruses 1, 2, 3, 4, 5, 7, 8, 11, 37, 53, 54, 56, 64, 81, and 85). The detection limits were presented as numerical values as determined by real-time polymerase chain reaction (PCR). Results The detection limit of the SAI kit for the adenovirus types ranged from 1.0 × 103 -5.0 × 10 4 copies/test (geometric mean, 4.7 × 10 3 ). SAI had a 10-250-fold lower detection limit than the four IC kits for all adenoviruses studied. There were also differences in detection limits among the adenovirus types for each kit. Discussion The detection limit of the SAI kit was drastically reduced because the silver-amplification reaction increased the color development sensitivity. The results revealed the high sensitivity of SAI for detecting adenoviruses and suggested its usefulness for conjunctivitis examination.
Tsuguto Fujimoto - One of the best experts on this subject based on the ideXlab platform.
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evaluation of a silver amplified Immunochromatography kit for adenoviral conjunctivitis
Journal of Medical Virology, 2019Co-Authors: Tsuguto Fujimoto, Tomoko Tsukaharakawamura, Nozomu Hanaoka, Eiichi Uchio, Masami Konagaya, Masaaki Kobayashi, Hisashi Nakagawa, Hiroshi Hatano, Hisatoshi KanekoAbstract:OBJECTIVE To compare and evaluate the sensitivity of a newly developed silver-amplified Immunochromatography (SAI) kit with various Immunochromatography (IC) kits for adenoviruses based on the detection limit (copies/test). METHODS An SAI kit and four ophthalmic IC kits were evaluated. The detection limits of the five kits were determined using the limiting dilution method for 15 conjunctivitis-associated adenoviruses (adenoviruses 1, 2, 3, 4, 5, 7, 8, 11, 37, 53, 54, 56, 64, 81, and 85). The detection limits were presented as numerical values as determined by real-time polymerase chain reaction (PCR). RESULTS The detection limit of the SAI kit for the adenovirus types ranged from 1.0 × 103 -5.0 × 10 4 copies/test (geometric mean, 4.7 × 10 3 ). SAI had a 10-250-fold lower detection limit than the four IC kits for all adenoviruses studied. There were also differences in detection limits among the adenovirus types for each kit. DISCUSSION The detection limit of the SAI kit was drastically reduced because the silver-amplification reaction increased the color development sensitivity. The results revealed the high sensitivity of SAI for detecting adenoviruses and suggested its usefulness for conjunctivitis examination.
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Evaluation of adenovirus amplified detection of immunochromatographic test using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis
Graefe's Archive for Clinical and Experimental Ophthalmology, 2019Co-Authors: Hironori Migita, Tomohiro Ueno, Yusuke Saeki, Nozomu Hanaoka, Tsuguto Fujimoto, Tomoko Tsukahara-kawamura, Eiichi UchioAbstract:Background We evaluated a novel silver amplification Immunochromatography test for rapid detection of adenovirus (AdV) antigen equipped with an automated reader system using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis. Methods Two kinds of immunochromatographic (IC) kits, a conventional IC kit for conjunctival scrapings (control kit) and an IC kit using tears including conjunctival exudate collected by pressing a filter paper strip on the conjunctiva (test kit), were tested on 90 patients who attended Migita Eye Clinic with suspected adenoviral conjunctivitis. The results of the test kits were automatically obtained by a specific reader, which was based on silver amplification Immunochromatography system, in 15 min. The detection of AdV was confirmed by real-time polymerase chain reaction (PCR) method, and typing was performed by direct sequencing. Comparative dilution assay was carried out with the two kits, using AdV type 3 and type 54 strains. Results The sensitivity of the control kit and test kit was 89.8% and 98.3%, respectively. The specificity of both kits was 100%. A significant difference in the sensitivities of the two IC kits against PCR positivity was observed ( P
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evaluation of adenovirus amplified detection of immunochromatographic test using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis
Graefes Archive for Clinical and Experimental Ophthalmology, 2019Co-Authors: Hironori Migita, Tomohiro Ueno, Tomoko Tsukaharakawamura, Yusuke Saeki, Nozomu Hanaoka, Tsuguto Fujimoto, Eiichi UchioAbstract:Background We evaluated a novel silver amplification Immunochromatography test for rapid detection of adenovirus (AdV) antigen equipped with an automated reader system using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis.
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Evaluation of a silver‐amplified Immunochromatography kit for adenoviral conjunctivitis
Journal of medical virology, 2019Co-Authors: Tsuguto Fujimoto, Nozomu Hanaoka, Eiichi Uchio, Tomoko Tsukahara-kawamura, Masami Konagaya, Masaaki Kobayashi, Hisashi Nakagawa, Hiroshi Hatano, Hisatoshi KanekoAbstract:Objective To compare and evaluate the sensitivity of a newly developed silver-amplified Immunochromatography (SAI) kit with various Immunochromatography (IC) kits for adenoviruses based on the detection limit (copies/test). Methods An SAI kit and four ophthalmic IC kits were evaluated. The detection limits of the five kits were determined using the limiting dilution method for 15 conjunctivitis-associated adenoviruses (adenoviruses 1, 2, 3, 4, 5, 7, 8, 11, 37, 53, 54, 56, 64, 81, and 85). The detection limits were presented as numerical values as determined by real-time polymerase chain reaction (PCR). Results The detection limit of the SAI kit for the adenovirus types ranged from 1.0 × 103 -5.0 × 10 4 copies/test (geometric mean, 4.7 × 10 3 ). SAI had a 10-250-fold lower detection limit than the four IC kits for all adenoviruses studied. There were also differences in detection limits among the adenovirus types for each kit. Discussion The detection limit of the SAI kit was drastically reduced because the silver-amplification reaction increased the color development sensitivity. The results revealed the high sensitivity of SAI for detecting adenoviruses and suggested its usefulness for conjunctivitis examination.
Nozomu Hanaoka - One of the best experts on this subject based on the ideXlab platform.
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evaluation of a silver amplified Immunochromatography kit for adenoviral conjunctivitis
Journal of Medical Virology, 2019Co-Authors: Tsuguto Fujimoto, Tomoko Tsukaharakawamura, Nozomu Hanaoka, Eiichi Uchio, Masami Konagaya, Masaaki Kobayashi, Hisashi Nakagawa, Hiroshi Hatano, Hisatoshi KanekoAbstract:OBJECTIVE To compare and evaluate the sensitivity of a newly developed silver-amplified Immunochromatography (SAI) kit with various Immunochromatography (IC) kits for adenoviruses based on the detection limit (copies/test). METHODS An SAI kit and four ophthalmic IC kits were evaluated. The detection limits of the five kits were determined using the limiting dilution method for 15 conjunctivitis-associated adenoviruses (adenoviruses 1, 2, 3, 4, 5, 7, 8, 11, 37, 53, 54, 56, 64, 81, and 85). The detection limits were presented as numerical values as determined by real-time polymerase chain reaction (PCR). RESULTS The detection limit of the SAI kit for the adenovirus types ranged from 1.0 × 103 -5.0 × 10 4 copies/test (geometric mean, 4.7 × 10 3 ). SAI had a 10-250-fold lower detection limit than the four IC kits for all adenoviruses studied. There were also differences in detection limits among the adenovirus types for each kit. DISCUSSION The detection limit of the SAI kit was drastically reduced because the silver-amplification reaction increased the color development sensitivity. The results revealed the high sensitivity of SAI for detecting adenoviruses and suggested its usefulness for conjunctivitis examination.
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Evaluation of adenovirus amplified detection of immunochromatographic test using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis
Graefe's Archive for Clinical and Experimental Ophthalmology, 2019Co-Authors: Hironori Migita, Tomohiro Ueno, Yusuke Saeki, Nozomu Hanaoka, Tsuguto Fujimoto, Tomoko Tsukahara-kawamura, Eiichi UchioAbstract:Background We evaluated a novel silver amplification Immunochromatography test for rapid detection of adenovirus (AdV) antigen equipped with an automated reader system using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis. Methods Two kinds of immunochromatographic (IC) kits, a conventional IC kit for conjunctival scrapings (control kit) and an IC kit using tears including conjunctival exudate collected by pressing a filter paper strip on the conjunctiva (test kit), were tested on 90 patients who attended Migita Eye Clinic with suspected adenoviral conjunctivitis. The results of the test kits were automatically obtained by a specific reader, which was based on silver amplification Immunochromatography system, in 15 min. The detection of AdV was confirmed by real-time polymerase chain reaction (PCR) method, and typing was performed by direct sequencing. Comparative dilution assay was carried out with the two kits, using AdV type 3 and type 54 strains. Results The sensitivity of the control kit and test kit was 89.8% and 98.3%, respectively. The specificity of both kits was 100%. A significant difference in the sensitivities of the two IC kits against PCR positivity was observed ( P
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evaluation of adenovirus amplified detection of immunochromatographic test using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis
Graefes Archive for Clinical and Experimental Ophthalmology, 2019Co-Authors: Hironori Migita, Tomohiro Ueno, Tomoko Tsukaharakawamura, Yusuke Saeki, Nozomu Hanaoka, Tsuguto Fujimoto, Eiichi UchioAbstract:Background We evaluated a novel silver amplification Immunochromatography test for rapid detection of adenovirus (AdV) antigen equipped with an automated reader system using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis.
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Evaluation of a silver‐amplified Immunochromatography kit for adenoviral conjunctivitis
Journal of medical virology, 2019Co-Authors: Tsuguto Fujimoto, Nozomu Hanaoka, Eiichi Uchio, Tomoko Tsukahara-kawamura, Masami Konagaya, Masaaki Kobayashi, Hisashi Nakagawa, Hiroshi Hatano, Hisatoshi KanekoAbstract:Objective To compare and evaluate the sensitivity of a newly developed silver-amplified Immunochromatography (SAI) kit with various Immunochromatography (IC) kits for adenoviruses based on the detection limit (copies/test). Methods An SAI kit and four ophthalmic IC kits were evaluated. The detection limits of the five kits were determined using the limiting dilution method for 15 conjunctivitis-associated adenoviruses (adenoviruses 1, 2, 3, 4, 5, 7, 8, 11, 37, 53, 54, 56, 64, 81, and 85). The detection limits were presented as numerical values as determined by real-time polymerase chain reaction (PCR). Results The detection limit of the SAI kit for the adenovirus types ranged from 1.0 × 103 -5.0 × 10 4 copies/test (geometric mean, 4.7 × 10 3 ). SAI had a 10-250-fold lower detection limit than the four IC kits for all adenoviruses studied. There were also differences in detection limits among the adenovirus types for each kit. Discussion The detection limit of the SAI kit was drastically reduced because the silver-amplification reaction increased the color development sensitivity. The results revealed the high sensitivity of SAI for detecting adenoviruses and suggested its usefulness for conjunctivitis examination.
Hironori Migita - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of adenovirus amplified detection of immunochromatographic test using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis
Graefe's Archive for Clinical and Experimental Ophthalmology, 2019Co-Authors: Hironori Migita, Tomohiro Ueno, Yusuke Saeki, Nozomu Hanaoka, Tsuguto Fujimoto, Tomoko Tsukahara-kawamura, Eiichi UchioAbstract:Background We evaluated a novel silver amplification Immunochromatography test for rapid detection of adenovirus (AdV) antigen equipped with an automated reader system using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis. Methods Two kinds of immunochromatographic (IC) kits, a conventional IC kit for conjunctival scrapings (control kit) and an IC kit using tears including conjunctival exudate collected by pressing a filter paper strip on the conjunctiva (test kit), were tested on 90 patients who attended Migita Eye Clinic with suspected adenoviral conjunctivitis. The results of the test kits were automatically obtained by a specific reader, which was based on silver amplification Immunochromatography system, in 15 min. The detection of AdV was confirmed by real-time polymerase chain reaction (PCR) method, and typing was performed by direct sequencing. Comparative dilution assay was carried out with the two kits, using AdV type 3 and type 54 strains. Results The sensitivity of the control kit and test kit was 89.8% and 98.3%, respectively. The specificity of both kits was 100%. A significant difference in the sensitivities of the two IC kits against PCR positivity was observed ( P
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evaluation of adenovirus amplified detection of immunochromatographic test using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis
Graefes Archive for Clinical and Experimental Ophthalmology, 2019Co-Authors: Hironori Migita, Tomohiro Ueno, Tomoko Tsukaharakawamura, Yusuke Saeki, Nozomu Hanaoka, Tsuguto Fujimoto, Eiichi UchioAbstract:Background We evaluated a novel silver amplification Immunochromatography test for rapid detection of adenovirus (AdV) antigen equipped with an automated reader system using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis.
Tomoko Tsukaharakawamura - One of the best experts on this subject based on the ideXlab platform.
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evaluation of a silver amplified Immunochromatography kit for adenoviral conjunctivitis
Journal of Medical Virology, 2019Co-Authors: Tsuguto Fujimoto, Tomoko Tsukaharakawamura, Nozomu Hanaoka, Eiichi Uchio, Masami Konagaya, Masaaki Kobayashi, Hisashi Nakagawa, Hiroshi Hatano, Hisatoshi KanekoAbstract:OBJECTIVE To compare and evaluate the sensitivity of a newly developed silver-amplified Immunochromatography (SAI) kit with various Immunochromatography (IC) kits for adenoviruses based on the detection limit (copies/test). METHODS An SAI kit and four ophthalmic IC kits were evaluated. The detection limits of the five kits were determined using the limiting dilution method for 15 conjunctivitis-associated adenoviruses (adenoviruses 1, 2, 3, 4, 5, 7, 8, 11, 37, 53, 54, 56, 64, 81, and 85). The detection limits were presented as numerical values as determined by real-time polymerase chain reaction (PCR). RESULTS The detection limit of the SAI kit for the adenovirus types ranged from 1.0 × 103 -5.0 × 10 4 copies/test (geometric mean, 4.7 × 10 3 ). SAI had a 10-250-fold lower detection limit than the four IC kits for all adenoviruses studied. There were also differences in detection limits among the adenovirus types for each kit. DISCUSSION The detection limit of the SAI kit was drastically reduced because the silver-amplification reaction increased the color development sensitivity. The results revealed the high sensitivity of SAI for detecting adenoviruses and suggested its usefulness for conjunctivitis examination.
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evaluation of adenovirus amplified detection of immunochromatographic test using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis
Graefes Archive for Clinical and Experimental Ophthalmology, 2019Co-Authors: Hironori Migita, Tomohiro Ueno, Tomoko Tsukaharakawamura, Yusuke Saeki, Nozomu Hanaoka, Tsuguto Fujimoto, Eiichi UchioAbstract:Background We evaluated a novel silver amplification Immunochromatography test for rapid detection of adenovirus (AdV) antigen equipped with an automated reader system using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis.
